]> git.donarmstrong.com Git - mothur.git/blobdiff - chopseqscommand.cpp
added modify names parameter to set.dir
[mothur.git] / chopseqscommand.cpp
index 2eabbc1099c40cab04f6de929014b326fddab6d4..96d7ae641b8b956694fe5ad12b9671c0279b302c 100644 (file)
 
 #include "chopseqscommand.h"
 #include "sequence.hpp"
+#include "removeseqscommand.h"
 
 //**********************************************************************************************************************
-
+vector<string> ChopSeqsCommand::setParameters(){       
+       try {
+               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","fasta",false,true,true); parameters.push_back(pfasta);
+        CommandParameter pname("name", "InputTypes", "", "", "NameCount", "none", "none","name",false,false,true); parameters.push_back(pname);
+        CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup", "none", "none","count",false,false,true); parameters.push_back(pcount);
+               CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup", "none", "none","group",false,false,true); parameters.push_back(pgroup);
+               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false,true); parameters.push_back(pprocessors);
+        CommandParameter pnumbases("numbases", "Number", "", "0", "", "", "","",false,true,true); parameters.push_back(pnumbases);
+               CommandParameter pcountgaps("countgaps", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(pcountgaps);
+               CommandParameter pshort("short", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(pshort);
+               CommandParameter pkeep("keep", "Multiple", "front-back", "front", "", "", "","",false,false); parameters.push_back(pkeep);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+               
+               vector<string> myArray;
+               for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
+               return myArray;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChopSeqsCommand", "setParameters");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+string ChopSeqsCommand::getHelpString(){       
+       try {
+               string helpString = "";
+               helpString += "The chop.seqs command reads a fasta file and outputs a .chop.fasta containing the trimmed sequences. Note: If a sequence is completely 'chopped', an accnos file will be created with the names of the sequences removed. \n";
+               helpString += "The chop.seqs command parameters are fasta, name, group, count, numbases, countgaps and keep. fasta is required unless you have a valid current fasta file. numbases is required.\n";
+               helpString += "The chop.seqs command should be in the following format: chop.seqs(fasta=yourFasta, numbases=yourNum, keep=yourKeep).\n";
+        helpString += "If you provide a name, group or count file any sequences removed from the fasta file will also be removed from those files.\n";
+               helpString += "The numbases parameter allows you to specify the number of bases you want to keep.\n";
+               helpString += "The keep parameter allows you to specify whether you want to keep the front or the back of your sequence, default=front.\n";
+               helpString += "The countgaps parameter allows you to specify whether you want to count gaps as bases, default=false.\n";
+               helpString += "The short parameter allows you to specify you want to keep sequences that are too short to chop, default=false.\n";
+               helpString += "The processors parameter allows you to specify how many processors you would like to use.  The default is 1. \n";
+        helpString += "For example, if you ran chop.seqs with numbases=200 and short=t, if a sequence had 100 bases mothur would keep the sequence rather than eliminate it.\n";
+               helpString += "Example chop.seqs(fasta=amazon.fasta, numbases=200, keep=front).\n";
+               helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n";
+               return helpString;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChopSeqsCommand", "getHelpString");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+string ChopSeqsCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "fasta") {  pattern = "[filename],chop.fasta"; }
+        else if (type == "name") {  pattern = "[filename],chop.names"; }
+        else if (type == "group") {  pattern = "[filename],chop.groups"; }
+        else if (type == "count") {  pattern = "[filename],chop.count_table"; } 
+        else if (type == "accnos") {  pattern = "[filename],chop.accnos"; } 
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "ChopSeqsCommand", "getOutputPattern");
+        exit(1);
+    }
+}
+//**********************************************************************************************************************
+ChopSeqsCommand::ChopSeqsCommand(){    
+       try {
+               abort = true; calledHelp = true; 
+               setParameters();
+               vector<string> tempOutNames;
+               outputTypes["fasta"] = tempOutNames;
+               outputTypes["accnos"] = tempOutNames;
+        outputTypes["name"] = tempOutNames;
+        outputTypes["group"] = tempOutNames;
+        outputTypes["count"] = tempOutNames;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChopSeqsCommand", "ChopSeqsCommand");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
 ChopSeqsCommand::ChopSeqsCommand(string option)  {
        try {
-               abort = false;
+               abort = false; calledHelp = false;   
                
                //allow user to run help
-               if(option == "help") { help(); abort = true; }
+               if(option == "help") { help(); abort = true; calledHelp = true; }
+               else if(option == "citation") { citation(); abort = true; calledHelp = true;}
                
                else {
-                       //valid paramters for this command
-                       string Array[] =  {"fasta","numbases","countgaps","keep","outputdir","inputdir"};
-                       vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+                       vector<string> myArray = setParameters();
                        
                        OptionParser parser(option);
                        map<string,string> parameters = parser.getParameters();
@@ -35,9 +117,14 @@ ChopSeqsCommand::ChopSeqsCommand(string option)  {
                                if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
                        }
                        
-                       //if the user changes the output directory command factory will send this info to us in the output parameter 
-                       outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = "";         }
-                       
+                       //initialize outputTypes
+                       vector<string> tempOutNames;
+                       outputTypes["fasta"] = tempOutNames;
+                       outputTypes["accnos"] = tempOutNames;
+            outputTypes["name"] = tempOutNames;
+            outputTypes["group"] = tempOutNames;
+            outputTypes["count"] = tempOutNames;
+               
                        //if the user changes the input directory command factory will send this info to us in the output parameter 
                        string inputDir = validParameter.validFile(parameters, "inputdir", false);              
                        if (inputDir == "not found"){   inputDir = "";          }
@@ -46,22 +133,83 @@ ChopSeqsCommand::ChopSeqsCommand(string option)  {
                                it = parameters.find("fasta");
                                //user has given a template file
                                if(it != parameters.end()){ 
-                                       path = hasPath(it->second);
+                                       path = m->hasPath(it->second);
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
                                }
+                
+                it = parameters.find("name");
+                               //user has given a template file
+                               if(it != parameters.end()){
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["name"] = inputDir + it->second;             }
+                               }
+                
+                it = parameters.find("group");
+                               //user has given a template file
+                               if(it != parameters.end()){
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["group"] = inputDir + it->second;            }
+                               }
+                
+                it = parameters.find("count");
+                               //user has given a template file
+                               if(it != parameters.end()){
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["count"] = inputDir + it->second;            }
+                               }
                        }
 
                        //check for required parameters
                        fastafile = validParameter.validFile(parameters, "fasta", true);
                        if (fastafile == "not open") { abort = true; }
-                       else if (fastafile == "not found") {  m->mothurOut("You must provide a fasta file."); m->mothurOutEndLine(); abort = true; }    
+                       else if (fastafile == "not found") {                            //if there is a current fasta file, use it
+                               fastafile = m->getFastaFile(); 
+                               if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
+                               else {  m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
+                       }else { m->setFastaFile(fastafile); }   
+                       
+                       namefile = validParameter.validFile(parameters, "name", true);
+                       if (namefile == "not open") { namefile = ""; abort = true; }
+                       else if (namefile == "not found") { namefile = ""; }
+                       else { m->setNameFile(namefile); }
+                       
+                       groupfile = validParameter.validFile(parameters, "group", true);
+                       if (groupfile == "not open") { groupfile = ""; abort = true; }
+                       else if (groupfile == "not found") { groupfile = ""; }
+                       else { m->setGroupFile(groupfile); }
+                               
+            countfile = validParameter.validFile(parameters, "count", true);
+                       if (countfile == "not open") { countfile = ""; abort = true; }
+                       else if (countfile == "not found") { countfile = "";  }
+                       else { m->setCountTableFile(countfile); }
+            
+            if ((namefile != "") && (countfile != "")) {
+                m->mothurOut("[ERROR]: you may only use one of the following: name or count."); m->mothurOutEndLine(); abort = true;
+            }
+                       
+            if ((groupfile != "") && (countfile != "")) {
+                m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true;
+            }
+
+                       //if the user changes the output directory command factory will send this info to us in the output parameter 
+                       outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
                        
                        string temp = validParameter.validFile(parameters, "numbases", false);  if (temp == "not found") { temp = "0"; } 
-                       convert(temp, numbases);   
+                       m->mothurConvert(temp, numbases);   
                        
+            temp = validParameter.validFile(parameters, "processors", false);  if (temp == "not found"){       temp = m->getProcessors();      }
+                       m->setProcessors(temp);
+                       m->mothurConvert(temp, processors);
+            
                        temp = validParameter.validFile(parameters, "countgaps", false);        if (temp == "not found") { temp = "f"; } 
-                       countGaps = isTrue(temp);   
+                       countGaps = m->isTrue(temp);  
+                       
+                       temp = validParameter.validFile(parameters, "short", false);    if (temp == "not found") { temp = "f"; } 
+                       Short = m->isTrue(temp);   
                
                        keep = validParameter.validFile(parameters, "keep", false);             if (keep == "not found") { keep = "front"; } 
                                
@@ -76,49 +224,303 @@ ChopSeqsCommand::ChopSeqsCommand(string option)  {
 }
 //**********************************************************************************************************************
 
-void ChopSeqsCommand::help(){
+int ChopSeqsCommand::execute(){
        try {
-               m->mothurOut("The chop.seqs command reads a fasta file and outputs a .chop.fasta containing the trimmed sequences.\n");
-               m->mothurOut("The chop.seqs command parameters are fasta, numbases, countgaps and keep. fasta and numbases are required required.\n");
-               m->mothurOut("The chop.seqs command should be in the following format: chop.seqs(fasta=yourFasta, numbases=yourNum, keep=yourKeep).\n");
-               m->mothurOut("The numbases parameter allows you to specify the number of bases you want to keep.\n");
-               m->mothurOut("The keep parameter allows you to specify whether you want to keep the front or the back of your sequence, default=front.\n");
-               m->mothurOut("The countgaps parameter allows you to specify whether you want to count gaps as bases, default=false.\n");
-               m->mothurOut("Example chop.seqs(fasta=amazon.fasta, numbases=200, keep=front).\n");
-               m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n");
+               
+               if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
+               
+        map<string, string> variables;
+        string thisOutputDir = outputDir;
+               if (outputDir == "") {  thisOutputDir += m->hasPath(fastafile);  }
+        variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(fastafile));
+        string outputFileName = getOutputFileName("fasta", variables);
+        outputNames.push_back(outputFileName); outputTypes["fasta"].push_back(outputFileName);
+        string outputFileNameAccnos = getOutputFileName("accnos", variables);        
+        
+        vector<unsigned long long> positions; 
+        vector<linePair> lines;
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+        positions = m->divideFile(fastafile, processors);
+        for (int i = 0; i < (positions.size()-1); i++) {       lines.push_back(linePair(positions[i], positions[(i+1)]));      }
+#else
+        int numSeqs = 0;
+        positions = m->setFilePosFasta(fastafile, numSeqs); 
+        if (positions.size() < processors) { processors = positions.size(); }
+               
+        //figure out how many sequences you have to process
+        int numSeqsPerProcessor = numSeqs / processors;
+        for (int i = 0; i < processors; i++) {
+            int startIndex =  i * numSeqsPerProcessor;
+            if(i == (processors - 1)){ numSeqsPerProcessor = numSeqs - i * numSeqsPerProcessor;        }
+            lines.push_back(linePair(positions[startIndex], numSeqsPerProcessor));
+        }
+#endif
+        
+        bool wroteAccnos = false;
+        if(processors == 1) {   wroteAccnos = driver(lines[0], fastafile, outputFileName, outputFileNameAccnos);        }
+        else                {   wroteAccnos = createProcesses(lines, fastafile, outputFileName, outputFileNameAccnos);  }
+        
+        if (m->control_pressed) {  return 0; }
+               
+        if (wroteAccnos) {
+            outputNames.push_back(outputFileNameAccnos); outputTypes["accnos"].push_back(outputFileNameAccnos);
+            
+             //use remove.seqs to create new name, group and count file
+            if ((countfile != "") || (namefile != "") || (groupfile != "")) {
+                string inputString = "accnos=" + outputFileNameAccnos;
+                
+                if (countfile != "") {  inputString += ", count=" + countfile;  }
+                else{
+                    if (namefile != "") {  inputString += ", name=" + namefile;  }
+                    if (groupfile != "") {  inputString += ", group=" + groupfile;  }
+                }
+                
+                m->mothurOut("/******************************************/"); m->mothurOutEndLine();
+                m->mothurOut("Running command: remove.seqs(" + inputString + ")"); m->mothurOutEndLine();
+                m->mothurCalling = true;
+                
+                Command* removeCommand = new RemoveSeqsCommand(inputString);
+                removeCommand->execute();
+                
+                map<string, vector<string> > filenames = removeCommand->getOutputFiles();
+                
+                delete removeCommand;
+                m->mothurCalling = false;
+                m->mothurOut("/******************************************/"); m->mothurOutEndLine();
+                
+                if (groupfile != "") {
+                    thisOutputDir = outputDir;
+                    if (outputDir == "") {  thisOutputDir += m->hasPath(groupfile);  }
+                    variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(groupfile));
+                    string outGroup = getOutputFileName("group", variables);
+                    m->renameFile(filenames["group"][0], outGroup);
+                    outputNames.push_back(outGroup); outputTypes["group"].push_back(outGroup);
+                }
+                
+                if (namefile != "") {
+                    thisOutputDir = outputDir;
+                    if (outputDir == "") {  thisOutputDir += m->hasPath(namefile);  }
+                    variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(namefile));
+                    string outName = getOutputFileName("name", variables);
+                    m->renameFile(filenames["name"][0], outName);
+                    outputNames.push_back(outName); outputTypes["name"].push_back(outName);
+                }
+                
+                if (countfile != "") {
+                    thisOutputDir = outputDir;
+                    if (outputDir == "") {  thisOutputDir += m->hasPath(countfile);  }
+                    variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile));
+                    string outCount = getOutputFileName("count", variables);
+                    m->renameFile(filenames["count"][0], outCount);
+                    outputNames.push_back(outCount); outputTypes["count"].push_back(outCount);
+                }
+            }
+        }
+               else {  m->mothurRemove(outputFileNameAccnos);  }
+               
+               //set fasta file as new current fastafile
+               string current = "";
+               itTypes = outputTypes.find("fasta");
+               if (itTypes != outputTypes.end()) {
+                       if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
+               }
+        
+               if (wroteAccnos) { //set accnos file as new current accnosfile
+                       itTypes = outputTypes.find("accnos");
+                       if (itTypes != outputTypes.end()) {
+                               if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setAccnosFile(current); }
+                       }
+            
+            itTypes = outputTypes.find("name");
+            if (itTypes != outputTypes.end()) {
+                if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setNameFile(current); }
+            }
+            
+            itTypes = outputTypes.find("group");
+            if (itTypes != outputTypes.end()) {
+                if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setGroupFile(current); }
+            }
+            
+            itTypes = outputTypes.find("count");
+            if (itTypes != outputTypes.end()) {
+                if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setCountTableFile(current); }
+            }
+               }
+               
+        m->mothurOutEndLine();
+               m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+               for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
+               m->mothurOutEndLine();
+               
+               return 0;               
        }
+
        catch(exception& e) {
-               m->errorOut(e, "ChopSeqsCommand", "help");
+               m->errorOut(e, "ChopSeqsCommand", "execute");
                exit(1);
        }
 }
-
-//**********************************************************************************************************************
-
-int ChopSeqsCommand::execute(){
+/**************************************************************************************************/
+bool ChopSeqsCommand::createProcesses(vector<linePair> lines, string filename, string outFasta, string outAccnos) {
        try {
+               int process = 1;
+               bool wroteAccnos = false;
+               vector<int> processIDS;
+        vector<string> nonBlankAccnosFiles;
+               
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+               
+               //loop through and create all the processes you want
+               while (process != processors) {
+                       int pid = fork();
+                       
+                       if (pid > 0) {
+                               processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
+                               process++;
+                       }else if (pid == 0){
+                               wroteAccnos = driver(lines[process], filename, outFasta + toString(getpid()) + ".temp", outAccnos + toString(getpid()) + ".temp");
+                               
+                               //pass numSeqs to parent
+                               ofstream out;
+                               string tempFile = fastafile + toString(getpid()) + ".bool.temp";
+                               m->openOutputFile(tempFile, out);
+                               out << wroteAccnos << endl;                             
+                               out.close();
+                               
+                               exit(0);
+                       }else { 
+                               m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); 
+                               for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+                               exit(0);
+                       }
+               }
                
-               if (abort == true) { return 0; }
+               //do your part
+               wroteAccnos = driver(lines[0], filename, outFasta, outAccnos);
+        
+               //force parent to wait until all the processes are done
+               for (int i=0;i<processIDS.size();i++) { 
+                       int temp = processIDS[i];
+                       wait(&temp);
+               }
                
-               string outputFileName = outputDir + getRootName(getSimpleName(fastafile)) + "chop.fasta";
-               string outputFileNameAccnos = outputDir + getRootName(getSimpleName(fastafile)) + "chop.accnos";
+        
+               if (wroteAccnos) { nonBlankAccnosFiles.push_back(outAccnos); }
+               else { m->mothurRemove(outAccnos); } //remove so other files can be renamed to it
+        
+               //parent reads in and combine Filter info
+               for (int i = 0; i < processIDS.size(); i++) {
+                       string tempFilename = fastafile + toString(processIDS[i]) + ".bool.temp";
+                       ifstream in;
+                       m->openInputFile(tempFilename, in);
+                       
+                       bool temp;
+                       in >> temp; m->gobble(in); 
+            if (temp) { wroteAccnos = temp; nonBlankAccnosFiles.push_back(outAccnos + toString(processIDS[i]) + ".temp");  }
+                       else { m->mothurRemove((outAccnos + toString(processIDS[i]) + ".temp"));  }
+            
+                       in.close();
+                       m->mothurRemove(tempFilename);
+               }
+#else
+               //////////////////////////////////////////////////////////////////////////////////////////////////////
+               //Windows version shared memory, so be careful when passing variables through the seqSumData struct. 
+               //Above fork() will clone, so memory is separate, but that's not the case with windows, 
+               //Taking advantage of shared memory to allow both threads to add info to vectors.
+               //////////////////////////////////////////////////////////////////////////////////////////////////////
                
-               ofstream out;
-               openOutputFile(outputFileName, out);
+               vector<chopData*> pDataArray; 
+               DWORD   dwThreadIdArray[processors-1];
+               HANDLE  hThreadArray[processors-1]; 
                
-               ofstream outAcc;
-               openOutputFile(outputFileNameAccnos, outAcc);
+               //Create processor worker threads.
+               for( int i=0; i<processors-1; i++ ){
+            
+            string extension = "";
+            if (i != 0) { extension = toString(i) + ".temp"; processIDS.push_back(i); }
+                       // Allocate memory for thread data.
+                       chopData* tempChop = new chopData(filename, (outFasta+extension), (outAccnos+extension), m, lines[i].start, lines[i].end, keep, countGaps, numbases, Short);
+                       pDataArray.push_back(tempChop);
+                       
+                       //MyChopThreadFunction is in header. It must be global or static to work with the threads.
+                       //default security attributes, thread function name, argument to thread function, use default creation flags, returns the thread identifier
+                       hThreadArray[i] = CreateThread(NULL, 0, MyChopThreadFunction, pDataArray[i], 0, &dwThreadIdArray[i]);   
+               }
                
-               ifstream in;
-               openInputFile(fastafile, in);
+        //do your part
+               wroteAccnos = driver(lines[processors-1], filename, (outFasta + toString(processors-1) + ".temp"), (outAccnos + toString(processors-1) + ".temp"));
+        processIDS.push_back(processors-1);
+        
+               //Wait until all threads have terminated.
+               WaitForMultipleObjects(processors-1, hThreadArray, TRUE, INFINITE);
                
-               bool wroteAccnos = false;
+        if (wroteAccnos) { nonBlankAccnosFiles.push_back(outAccnos); }
+               else { m->mothurRemove(outAccnos); } //remove so other files can be renamed to it
+
+               //Close all thread handles and free memory allocations.
+               for(int i=0; i < pDataArray.size(); i++){
+            if (pDataArray[i]->wroteAccnos) { wroteAccnos = pDataArray[i]->wroteAccnos; nonBlankAccnosFiles.push_back(outAccnos + toString(processIDS[i]) + ".temp");  }
+                       else { m->mothurRemove((outAccnos + toString(processIDS[i]) + ".temp"));  }
+            //check to make sure the process finished
+            if (pDataArray[i]->count != pDataArray[i]->end) {
+                m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end) + " sequences assigned to it, quitting. \n"); m->control_pressed = true; 
+            }
+                       CloseHandle(hThreadArray[i]);
+                       delete pDataArray[i];
+               }
+#endif         
+                
+               for (int i = 0; i < processIDS.size(); i++) {
+                       m->appendFiles((outFasta + toString(processIDS[i]) + ".temp"), outFasta);
+                       m->mothurRemove((outFasta + toString(processIDS[i]) + ".temp"));
+               }
                
-               while (!in.eof()) {
+        if (nonBlankAccnosFiles.size() != 0) { 
+                       m->renameFile(nonBlankAccnosFiles[0], outAccnos);
                        
-                       Sequence seq(in);
+                       for (int h=1; h < nonBlankAccnosFiles.size(); h++) {
+                               m->appendFiles(nonBlankAccnosFiles[h], outAccnos);
+                               m->mothurRemove(nonBlankAccnosFiles[h]);
+                       }
+               }else { //recreate the accnosfile if needed
+                       ofstream out;
+                       m->openOutputFile(outAccnos, out);
+                       out.close();
+               }
+
+               return wroteAccnos;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChopSeqsCommand", "createProcesses");
+               exit(1);
+       }
+}
+/**************************************************************************************/
+bool ChopSeqsCommand::driver(linePair filePos, string filename, string outFasta, string outAccnos) {   
+       try {
+               
+               ofstream out;
+               m->openOutputFile(outFasta, out);
+        
+        ofstream outAcc;
+               m->openOutputFile(outAccnos, outAcc);
+        
+               ifstream in;
+               m->openInputFile(filename, in);
+        
+               in.seekg(filePos.start);
+        
+               bool done = false;
+        bool wroteAccnos = false;
+               int count = 0;
+        
+               while (!done) {
+            
+                       if (m->control_pressed) { in.close(); out.close(); return 1; }
+            
+                       Sequence seq(in); m->gobble(in);
                        
-                       if (m->control_pressed) { in.close(); out.close(); outAcc.close(); remove(outputFileName.c_str()); remove(outputFileNameAccnos.c_str()); return 0;  }
+                       if (m->control_pressed) {  in.close(); out.close(); outAcc.close(); m->mothurRemove(outFasta); m->mothurRemove(outAccnos); return 0;  }
                        
                        if (seq.getName() != "") {
                                string newSeqString = getChopped(seq);
@@ -130,26 +532,31 @@ int ChopSeqsCommand::execute(){
                                        outAcc << seq.getName() << endl;
                                        wroteAccnos = true;
                                }
+                count++;
                        }
+                       
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+            unsigned long long pos = in.tellg();
+            if ((pos == -1) || (pos >= filePos.end)) { break; }
+#else
+            if (in.eof()) { break; }
+#endif
+            //report progress
+                       if((count) % 1000 == 0){        m->mothurOut(toString(count)); m->mothurOutEndLine();           }
+                       
                }
-               in.close();
-               out.close();
-               outAcc.close();
-               
-               m->mothurOutEndLine();
-               m->mothurOut("Output File Name: "); m->mothurOutEndLine();
-               m->mothurOut(outputFileName); m->mothurOutEndLine();    
-               
-               if (wroteAccnos) { m->mothurOut(outputFileNameAccnos); m->mothurOutEndLine();  }
-               else {  remove(outputFileNameAccnos.c_str());  }
+               //report progress
+               if((count) % 1000 != 0){        m->mothurOut(toString(count)); m->mothurOutEndLine();           }
+
                
-               m->mothurOutEndLine();
+               in.close();
+        out.close();
+        outAcc.close();
                
-               return 0;               
+               return wroteAccnos;
        }
-
        catch(exception& e) {
-               m->errorOut(e, "ChopSeqsCommand", "execute");
+               m->errorOut(e, "ChopSeqsCommand", "driver");
                exit(1);
        }
 }
@@ -171,7 +578,7 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        
                                        for (int i = 0; i < temp.length(); i++) {
                                                //eliminate N's
-                                               if (toupper(temp[i]) == 'N') { temp[i] == '.'; }
+                                               if (toupper(temp[i]) == 'N') { temp[i] = '.'; }
                                                
                                                numBasesCounted++; 
                                                
@@ -179,10 +586,11 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        }
                                        
                                        if (stopSpot == 0) { temp = ""; }
-                                       else {  temp = temp.substr(0, stopSpot);  }
+                                       else {  temp = temp.substr(0, stopSpot+1);  }
                                                        
-                               }else { temp = ""; } //sequence too short
-                               
+                               }else { 
+                                       if (!Short) { temp = ""; } //sequence too short
+                               }
                        }else { //you are keeping the back
                                int tempLength = temp.length();
                                if (tempLength > numbases) { //you have enough bases to remove some
@@ -192,7 +600,7 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        
                                        for (int i = (temp.length()-1); i >= 0; i--) {
                                                //eliminate N's
-                                               if (toupper(temp[i]) == 'N') { temp[i] == '.'; }
+                                               if (toupper(temp[i]) == 'N') { temp[i] = '.'; }
                                                
                                                numBasesCounted++; 
 
@@ -201,8 +609,9 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                
                                        if (stopSpot == 0) { temp = ""; }
                                        else {  temp = temp.substr(stopSpot+1);  }
+                               }else { 
+                                       if (!Short) { temp = ""; } //sequence too short
                                }
-                               else {  temp = ""; } //sequence too short
                        }
 
                }else{
@@ -219,7 +628,7 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        for (int i = 0; i < temp.length(); i++) {
                                                //eliminate N's
                                                if (toupper(temp[i]) == 'N') { 
-                                                       temp[i] == '.'; 
+                                                       temp[i] = '.'; 
                                                        tempLength--;
                                                        if (tempLength < numbases) { stopSpot = 0; break; }
                                                }
@@ -230,10 +639,11 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        }
                                        
                                        if (stopSpot == 0) { temp = ""; }
-                                       else {  temp = temp.substr(0, stopSpot);  }
+                                       else {  temp = temp.substr(0, stopSpot+1);  }
                                                        
-                               }else { temp = ""; } //sequence too short
-                               
+                               }else { 
+                                       if (!Short) { temp = ""; } //sequence too short
+                               }                               
                        }else { //you are keeping the back
                                int tempLength = tempUnaligned.length();
                                if (tempLength > numbases) { //you have enough bases to remove some
@@ -244,7 +654,7 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        for (int i = (temp.length()-1); i >= 0; i--) {
                                                //eliminate N's
                                                if (toupper(temp[i]) == 'N') { 
-                                                       temp[i] == '.'; 
+                                                       temp[i] = '.'; 
                                                        tempLength--;
                                                        if (tempLength < numbases) { stopSpot = 0; break; }
                                                }
@@ -255,9 +665,10 @@ string ChopSeqsCommand::getChopped(Sequence seq) {
                                        }
                                
                                        if (stopSpot == 0) { temp = ""; }
-                                       else {  temp = temp.substr(stopSpot+1);  }
+                                       else {  temp = temp.substr(stopSpot);  }
+                               }else { 
+                                       if (!Short) { temp = ""; } //sequence too short
                                }
-                               else {  temp = ""; } //sequence too short
                        }
                }