]> git.donarmstrong.com Git - mothur.git/blobdiff - chimerapintailcommand.cpp
testing 1.14.0
[mothur.git] / chimerapintailcommand.cpp
index 8dffd3a222565f39f6b029e96ce65afd6c47a4e4..b355f6da6ac5839a054d4503e4dbb4f43237c23a 100644 (file)
 #include "chimerapintailcommand.h"
 #include "pintail.h"
 
+//**********************************************************************************************************************
+vector<string> ChimeraPintailCommand::getValidParameters(){    
+       try {
+               string AlignArray[] =  {"fasta","filter","processors","window" ,"increment","template","conservation","quantile","mask","outputdir","inputdir"};
+               vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
+               return myArray;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraPintailCommand", "getValidParameters");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+ChimeraPintailCommand::ChimeraPintailCommand(){        
+       try {
+               vector<string> tempOutNames;
+               outputTypes["chimera"] = tempOutNames;
+               outputTypes["accnos"] = tempOutNames;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraPintailCommand", "ChimeraPintailCommand");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+vector<string> ChimeraPintailCommand::getRequiredParameters(){ 
+       try {
+               string AlignArray[] =  {"template","fasta"};
+               vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
+               return myArray;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraPintailCommand", "getRequiredParameters");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+vector<string> ChimeraPintailCommand::getRequiredFiles(){      
+       try {
+               vector<string> myArray;
+               return myArray;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraPintailCommand", "getRequiredFiles");
+               exit(1);
+       }
+}
 //***************************************************************************************************************
-
 ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
        try {
                abort = false;
@@ -27,7 +73,7 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                        OptionParser parser(option);
                        map<string,string> parameters = parser.getParameters();
                        
-                       ValidParameters validParameter;
+                       ValidParameters validParameter("chimera.pintail");
                        map<string,string>::iterator it;
                        
                        //check to make sure all parameters are valid for command
@@ -35,6 +81,10 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                                if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
                        }
                        
+                       vector<string> tempOutNames;
+                       outputTypes["chimera"] = tempOutNames;
+                       outputTypes["accnos"] = tempOutNames;
+               
                        //if the user changes the input directory command factory will send this info to us in the output parameter 
                        inputDir = validParameter.validFile(parameters, "inputdir", false);             
                        if (inputDir == "not found"){   inputDir = "";          }
@@ -43,7 +93,7 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                                it = parameters.find("template");
                                //user has given a template file
                                if(it != parameters.end()){ 
-                                       path = hasPath(it->second);
+                                       path = m->hasPath(it->second);
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["template"] = inputDir + it->second;         }
                                }
@@ -51,7 +101,7 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                                it = parameters.find("conservation");
                                //user has given a template file
                                if(it != parameters.end()){ 
-                                       path = hasPath(it->second);
+                                       path = m->hasPath(it->second);
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["conservation"] = inputDir + it->second;             }
                                }
@@ -59,7 +109,7 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                                it = parameters.find("quantile");
                                //user has given a template file
                                if(it != parameters.end()){ 
-                                       path = hasPath(it->second);
+                                       path = m->hasPath(it->second);
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["quantile"] = inputDir + it->second;         }
                                }
@@ -70,12 +120,12 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                        fastafile = validParameter.validFile(parameters, "fasta", false);
                        if (fastafile == "not found") { fastafile = ""; m->mothurOut("fasta is a required parameter for the chimera.pintail command."); m->mothurOutEndLine(); abort = true;  }
                        else { 
-                               splitAtDash(fastafile, fastaFileNames);
+                               m->splitAtDash(fastafile, fastaFileNames);
                                
                                //go through files and make sure they are good, if not, then disregard them
                                for (int i = 0; i < fastaFileNames.size(); i++) {
                                        if (inputDir != "") {
-                                               string path = hasPath(fastaFileNames[i]);
+                                               string path = m->hasPath(fastaFileNames[i]);
                                                //if the user has not given a path then, add inputdir. else leave path alone.
                                                if (path == "") {       fastaFileNames[i] = inputDir + fastaFileNames[i];               }
                                        }
@@ -83,30 +133,35 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                                        int ableToOpen;
                                        ifstream in;
                                        
-                                       #ifdef USE_MPI  
-                                               int pid;
-                                               MPI_Comm_size(MPI_COMM_WORLD, &processors); //set processors to the number of mpi processes running
-                                               MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+                                       ableToOpen = m->openInputFile(fastaFileNames[i], in, "noerror");
                                
-                                               if (pid == 0) {
-                                       #endif
-
-                                       ableToOpen = openInputFile(fastaFileNames[i], in);
-                                       in.close();
+                                       //if you can't open it, try default location
+                                       if (ableToOpen == 1) {
+                                               if (m->getDefaultPath() != "") { //default path is set
+                                                       string tryPath = m->getDefaultPath() + m->getSimpleName(fastaFileNames[i]);
+                                                       m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
+                                                       ifstream in2;
+                                                       ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                       in2.close();
+                                                       fastaFileNames[i] = tryPath;
+                                               }
+                                       }
                                        
-                                       #ifdef USE_MPI  
-                                                       for (int j = 1; j < processors; j++) {
-                                                               MPI_Send(&ableToOpen, 1, MPI_INT, j, 2001, MPI_COMM_WORLD); 
-                                                       }
-                                               }else{
-                                                       MPI_Status status;
-                                                       MPI_Recv(&ableToOpen, 1, MPI_INT, 0, 2001, MPI_COMM_WORLD, &status);
+                                       if (ableToOpen == 1) {
+                                               if (m->getOutputDir() != "") { //default path is set
+                                                       string tryPath = m->getOutputDir() + m->getSimpleName(fastaFileNames[i]);
+                                                       m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+                                                       ifstream in2;
+                                                       ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                       in2.close();
+                                                       fastaFileNames[i] = tryPath;
                                                }
-                                               
-                                       #endif
+                                       }
 
+                                       in.close();
+                                       
                                        if (ableToOpen == 1) { 
-                                               m->mothurOut(fastaFileNames[i] + " will be disregarded."); m->mothurOutEndLine(); 
+                                               m->mothurOut("Unable to open " + fastaFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine(); 
                                                //erase from file list
                                                fastaFileNames.erase(fastaFileNames.begin()+i);
                                                i--;
@@ -119,7 +174,7 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                        
                        string temp;
                        temp = validParameter.validFile(parameters, "filter", false);                   if (temp == "not found") { temp = "F"; }
-                       filter = isTrue(temp);
+                       filter = m->isTrue(temp);
                        
                        temp = validParameter.validFile(parameters, "processors", false);               if (temp == "not found") { temp = "1"; }
                        convert(temp, processors);
@@ -134,23 +189,46 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                        if (maskfile == "not found") { maskfile = "";  }        
                        else if (maskfile != "default")  { 
                                if (inputDir != "") {
-                                       string path = hasPath(maskfile);
+                                       string path = m->hasPath(maskfile);
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       maskfile = inputDir + maskfile;         }
                                }
 
                                ifstream in;
-                               int     ableToOpen = openInputFile(maskfile, in);
-                               if (ableToOpen == 1) { abort = true; }
+                               int     ableToOpen = m->openInputFile(maskfile, in, "no error");
+                               if (ableToOpen == 1) { 
+                                       if (m->getDefaultPath() != "") { //default path is set
+                                                       string tryPath = m->getDefaultPath() + m->getSimpleName(maskfile);
+                                                       m->mothurOut("Unable to open " + maskfile + ". Trying default " + tryPath); m->mothurOutEndLine();
+                                                       ifstream in2;
+                                                       ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                       in2.close();
+                                                       maskfile = tryPath;
+                                       }
+                               }
+                               
+                               if (ableToOpen == 1) {
+                                               if (m->getOutputDir() != "") { //default path is set
+                                                       string tryPath = m->getOutputDir() + m->getSimpleName(maskfile);
+                                                       m->mothurOut("Unable to open " + maskfile + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+                                                       ifstream in2;
+                                                       ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                       in2.close();
+                                                       maskfile = tryPath;
+                                               }
+                               }
+                               
                                in.close();
+                                       
+                               if (ableToOpen == 1) { 
+                                               m->mothurOut("Unable to open " + maskfile + "."); m->mothurOutEndLine(); 
+                                               abort = true;
+                               }
                        }
 
                        
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
-                       outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  
-                               outputDir = ""; 
-                               outputDir += hasPath(fastafile); //if user entered a file with a path then preserve it  
-                       }
+                       outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
                
                        templatefile = validParameter.validFile(parameters, "template", true);
                        if (templatefile == "not open") { abort = true; }
@@ -161,14 +239,28 @@ ChimeraPintailCommand::ChimeraPintailCommand(string option)  {
                        else if (consfile == "not found") { 
                                consfile = "";  
                                //check for consfile
-                               string tempConsFile = getRootName(inputDir + getSimpleName(templatefile)) + "freq";
+                               string tempConsFile = m->getRootName(inputDir + m->getSimpleName(templatefile)) + "freq";
                                ifstream FileTest(tempConsFile.c_str());
-                               if(FileTest){   m->mothurOut("I found " + tempConsFile + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  consfile = tempConsFile;  FileTest.close();     }
+                               if(FileTest){   
+                                       bool GoodFile = m->checkReleaseVersion(FileTest, m->getVersion());
+                                       if (GoodFile) {  
+                                               m->mothurOut("I found " + tempConsFile + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  consfile = tempConsFile;  FileTest.close();     
+                                       }
+                               }else {
+                                       string tempConsFile = m->getDefaultPath() + m->getRootName(m->getSimpleName(templatefile)) + "freq";
+                                       ifstream FileTest2(tempConsFile.c_str());
+                                       if(FileTest2){  
+                                               bool GoodFile = m->checkReleaseVersion(FileTest2, m->getVersion());
+                                               if (GoodFile) {  
+                                                       m->mothurOut("I found " + tempConsFile + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  consfile = tempConsFile;  FileTest2.close();    
+                                               }
+                                       }
+                               }
                        }       
                        
                        quanfile = validParameter.validFile(parameters, "quantile", true);
                        if (quanfile == "not open") { abort = true; }
-                       else if (quanfile == "not found") { quanfile = "";  }
+                       else if (quanfile == "not found") { quanfile = ""; }
                }
        }
        catch(exception& e) {
@@ -231,27 +323,53 @@ int ChimeraPintailCommand::execute(){
                        //check for quantile to save the time
                        string tempQuan = "";
                        if ((!filter) && (maskfile == "")) {
-                               tempQuan = inputDir + getRootName(getSimpleName(templatefile)) + "pintail.quan";
+                               tempQuan = inputDir + m->getRootName(m->getSimpleName(templatefile)) + "pintail.quan";
                        }else if ((!filter) && (maskfile != "")) { 
-                               tempQuan = inputDir + getRootName(getSimpleName(templatefile)) + "pintail.masked.quan";
+                               tempQuan = inputDir + m->getRootName(m->getSimpleName(templatefile)) + "pintail.masked.quan";
                        }else if ((filter) && (maskfile != "")) { 
-                               tempQuan = inputDir + getRootName(getSimpleName(templatefile)) + "pintail.filtered." + getSimpleName(getRootName(fastaFileNames[s])) + "masked.quan";
+                               tempQuan = inputDir + m->getRootName(m->getSimpleName(templatefile)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "masked.quan";
                        }else if ((filter) && (maskfile == "")) { 
-                               tempQuan = inputDir + getRootName(getSimpleName(templatefile)) + "pintail.filtered." + getSimpleName(getRootName(fastaFileNames[s])) + "quan";
+                               tempQuan = inputDir + m->getRootName(m->getSimpleName(templatefile)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "quan";
                        }
                        
                        ifstream FileTest(tempQuan.c_str());
-                       if(FileTest){   m->mothurOut("I found " + tempQuan + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  quanfile = tempQuan;  FileTest.close();     }
+                       if(FileTest){   
+                               bool GoodFile = m->checkReleaseVersion(FileTest, m->getVersion());
+                               if (GoodFile) {  
+                                       m->mothurOut("I found " + tempQuan + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  quanfile = tempQuan;  FileTest.close();     
+                               }
+                       }else {
+                               string tryPath = m->getDefaultPath();
+                               string tempQuan = "";
+                               if ((!filter) && (maskfile == "")) {
+                                       tempQuan = tryPath + m->getRootName(m->getSimpleName(templatefile)) + "pintail.quan";
+                               }else if ((!filter) && (maskfile != "")) { 
+                                       tempQuan = tryPath + m->getRootName(m->getSimpleName(templatefile)) + "pintail.masked.quan";
+                               }else if ((filter) && (maskfile != "")) { 
+                                       tempQuan = tryPath + m->getRootName(m->getSimpleName(templatefile)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "masked.quan";
+                               }else if ((filter) && (maskfile == "")) { 
+                                       tempQuan = tryPath + m->getRootName(m->getSimpleName(templatefile)) + "pintail.filtered." + m->getSimpleName(m->getRootName(fastaFileNames[s])) + "quan";
+                               }
+                               
+                               ifstream FileTest2(tempQuan.c_str());
+                               if(FileTest2){  
+                                       bool GoodFile = m->checkReleaseVersion(FileTest2, m->getVersion());
+                                       if (GoodFile) {  
+                                               m->mothurOut("I found " + tempQuan + " in your input file directory. I will use it to save time."); m->mothurOutEndLine();  quanfile = tempQuan;  FileTest2.close();    
+                                       }
+                               }
+                       }
                        
                        chimera = new Pintail(fastaFileNames[s], templatefile, filter, processors, maskfile, consfile, quanfile, window, increment, outputDir);
                        
+                       if (outputDir == "") { outputDir = m->hasPath(fastaFileNames[s]);  }//if user entered a file with a path then preserve it
                        string outputFileName, accnosFileName;
                        if (maskfile != "") {
-                               outputFileName = outputDir + getRootName(getSimpleName(fastaFileNames[s])) + maskfile + ".pintail.chimeras";
-                               accnosFileName = outputDir + getRootName(getSimpleName(fastaFileNames[s])) + maskfile + ".pintail.accnos";
+                               outputFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + m->getSimpleName(m->getRootName(maskfile)) + ".pintail.chimeras";
+                               accnosFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + m->getSimpleName(m->getRootName(maskfile)) + ".pintail.accnos";
                        }else {
-                               outputFileName = outputDir + getRootName(getSimpleName(fastaFileNames[s]))  + "pintail.chimeras";
-                               accnosFileName = outputDir + getRootName(getSimpleName(fastaFileNames[s]))  + "pintail.accnos";
+                               outputFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s]))  + "pintail.chimeras";
+                               accnosFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s]))  + "pintail.accnos";
                        }
                        
                        if (m->control_pressed) { delete chimera; for (int j = 0; j < outputNames.size(); j++) {        remove(outputNames[j].c_str()); }  return 0;    }
@@ -266,7 +384,7 @@ int ChimeraPintailCommand::execute(){
                #ifdef USE_MPI
                        int pid, end, numSeqsPerProcessor; 
                                int tag = 2001;
-                               vector<long> MPIPos;
+                               vector<unsigned long int> MPIPos;
                                
                                MPI_Status status; 
                                MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
@@ -292,11 +410,11 @@ int ChimeraPintailCommand::execute(){
                                MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI);
                                MPI_File_open(MPI_COMM_WORLD, outAccnosFilename, outMode, MPI_INFO_NULL, &outMPIAccnos);
                                
-                               if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
+                               if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {      remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
 
                                if (pid == 0) { //you are the root process 
                                                                
-                                       MPIPos = setFilePosFasta(fastaFileNames[s], numSeqs); //fills MPIPos, returns numSeqs
+                                       MPIPos = m->setFilePosFasta(fastaFileNames[s], numSeqs); //fills MPIPos, returns numSeqs
                                        
                                        //send file positions to all processes
                                        for(int i = 1; i < processors; i++) { 
@@ -312,7 +430,7 @@ int ChimeraPintailCommand::execute(){
                                        //do your part
                                        driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, MPIPos);
                                        
-                                       if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  remove(outputFileName.c_str());  remove(accnosFileName.c_str());  for (int j = 0; j < outputNames.size(); j++) { remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
+                                       if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  remove(outputFileName.c_str());  remove(accnosFileName.c_str());  for (int j = 0; j < outputNames.size(); j++) {    remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
                                        
                                }else{ //you are a child process
                                        MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
@@ -327,7 +445,7 @@ int ChimeraPintailCommand::execute(){
                                        //do your part
                                        driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, MPIPos);
                                        
-                                       if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
+                                       if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {      remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
                                }
                                
                                //close files 
@@ -336,77 +454,44 @@ int ChimeraPintailCommand::execute(){
                                MPI_File_close(&outMPIAccnos);
                                MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
                #else
-               
+                       vector<unsigned long int> positions = m->divideFile(fastaFileNames[s], processors);
+                               
+                       for (int i = 0; i < (positions.size()-1); i++) {
+                               lines.push_back(new linePair(positions[i], positions[(i+1)]));
+                       }       
+                       
                        //break up file
                        #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
                                if(processors == 1){
-                                       ifstream inFASTA;
-                                       openInputFile(fastaFileNames[s], inFASTA);
-                                       getNumSeqs(inFASTA, numSeqs);
-                                       inFASTA.close();
-                                       
-                                       lines.push_back(new linePair(0, numSeqs));
-                                       
-                                       driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
+               
+                                       numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
                                        
-                                       if (m->control_pressed) { 
-                                               remove(outputFileName.c_str()); 
-                                               remove(accnosFileName.c_str());
-                                               for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } 
-                                               for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
-                                               delete chimera;
-                                               return 0;
-                                       }
+                                       if (m->control_pressed) { outputTypes.clear(); remove(outputFileName.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
                                        
                                }else{
-                                       vector<unsigned long int> positions;
                                        processIDS.resize(0);
                                        
-                                       ifstream inFASTA;
-                                       openInputFile(fastaFileNames[s], inFASTA);
-                                       
-                                       string input;
-                                       while(!inFASTA.eof()){
-                                               input = getline(inFASTA);
-                                               if (input.length() != 0) {
-                                                       if(input[0] == '>'){    unsigned long int pos = inFASTA.tellg(); positions.push_back(pos - input.length() - 1); }
-                                               }
-                                       }
-                                       inFASTA.close();
-                                       
-                                       numSeqs = positions.size();
-                                       
-                                       int numSeqsPerProcessor = numSeqs / processors;
-                                       
-                                       for (int i = 0; i < processors; i++) {
-                                               unsigned long int startPos = positions[ i * numSeqsPerProcessor ];
-                                               if(i == processors - 1){
-                                                       numSeqsPerProcessor = numSeqs - i * numSeqsPerProcessor;
-                                               }
-                                               lines.push_back(new linePair(startPos, numSeqsPerProcessor));
-                                       }
-                                       
-                                       createProcesses(outputFileName, fastaFileNames[s], accnosFileName); 
+                                       numSeqs = createProcesses(outputFileName, fastaFileNames[s], accnosFileName); 
                                
                                        rename((outputFileName + toString(processIDS[0]) + ".temp").c_str(), outputFileName.c_str());
                                        rename((accnosFileName + toString(processIDS[0]) + ".temp").c_str(), accnosFileName.c_str());
                                                
                                        //append output files
                                        for(int i=1;i<processors;i++){
-                                               appendFiles((outputFileName + toString(processIDS[i]) + ".temp"), outputFileName);
+                                               m->appendFiles((outputFileName + toString(processIDS[i]) + ".temp"), outputFileName);
                                                remove((outputFileName + toString(processIDS[i]) + ".temp").c_str());
                                        }
                                        
                                        //append output files
                                        for(int i=1;i<processors;i++){
-                                               appendFiles((accnosFileName + toString(processIDS[i]) + ".temp"), accnosFileName);
+                                               m->appendFiles((accnosFileName + toString(processIDS[i]) + ".temp"), accnosFileName);
                                                remove((accnosFileName + toString(processIDS[i]) + ".temp").c_str());
                                        }
                                                                                
                                        if (m->control_pressed) { 
                                                remove(outputFileName.c_str()); 
                                                remove(accnosFileName.c_str());
-                                               for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } 
+                                               for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } outputTypes.clear();
                                                for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
                                                delete chimera;
                                                return 0;
@@ -414,22 +499,9 @@ int ChimeraPintailCommand::execute(){
                                }
 
                        #else
-                               ifstream inFASTA;
-                               openInputFile(fastaFileNames[s], inFASTA);
-                               getNumSeqs(inFASTA, numSeqs);
-                               inFASTA.close();
-                               lines.push_back(new linePair(0, numSeqs));
+                               numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
                                
-                               driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
-                               
-                               if (m->control_pressed) { 
-                                               remove(outputFileName.c_str()); 
-                                               remove(accnosFileName.c_str());
-                                               for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } 
-                                               for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
-                                               delete chimera;
-                                               return 0;
-                               }
+                               if (m->control_pressed) { outputTypes.clear(); remove(outputFileName.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
                        #endif
                        
                #endif  
@@ -437,8 +509,8 @@ int ChimeraPintailCommand::execute(){
                        delete chimera;
                        for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
                        
-                       outputNames.push_back(outputFileName);
-                       outputNames.push_back(accnosFileName); 
+                       outputNames.push_back(outputFileName); outputTypes["chimera"].push_back(outputFileName);
+                       outputNames.push_back(accnosFileName); outputTypes["accnos"].push_back(accnosFileName);
                        
                        m->mothurOutEndLine();
                        m->mothurOutEndLine(); m->mothurOut("It took " + toString(time(NULL) - start) + " secs to check " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
@@ -459,24 +531,27 @@ int ChimeraPintailCommand::execute(){
 }
 //**********************************************************************************************************************
 
-int ChimeraPintailCommand::driver(linePair* line, string outputFName, string filename, string accnos){
+int ChimeraPintailCommand::driver(linePair* filePos, string outputFName, string filename, string accnos){
        try {
                ofstream out;
-               openOutputFile(outputFName, out);
+               m->openOutputFile(outputFName, out);
                
                ofstream out2;
-               openOutputFile(accnos, out2);
+               m->openOutputFile(accnos, out2);
                
                ifstream inFASTA;
-               openInputFile(filename, inFASTA);
+               m->openInputFile(filename, inFASTA);
 
-               inFASTA.seekg(line->start);
-               
-               for(int i=0;i<line->numSeqs;i++){
-               
+               inFASTA.seekg(filePos->start);
+
+               bool done = false;
+               int count = 0;
+       
+               while (!done) {
+                               
                        if (m->control_pressed) {       return 1;       }
                
-                       Sequence* candidateSeq = new Sequence(inFASTA);  gobble(inFASTA);
+                       Sequence* candidateSeq = new Sequence(inFASTA);  m->gobble(inFASTA);
                                
                        if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
                                
@@ -491,20 +566,28 @@ int ChimeraPintailCommand::driver(linePair* line, string outputFName, string fil
                                        //print results
                                        chimera->print(out, out2);
                                }
+                               count++;
                        }
                        delete candidateSeq;
                        
+                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+                               unsigned long int pos = inFASTA.tellg();
+                               if ((pos == -1) || (pos >= filePos->end)) { break; }
+                       #else
+                               if (inFASTA.eof()) { break; }
+                       #endif
+                       
                        //report progress
-                       if((i+1) % 100 == 0){   m->mothurOut("Processing sequence: " + toString(i+1)); m->mothurOutEndLine();           }
+                       if((count) % 100 == 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
                }
                //report progress
-               if((line->numSeqs) % 100 != 0){ m->mothurOut("Processing sequence: " + toString(line->numSeqs)); m->mothurOutEndLine();         }
+               if((count) % 100 != 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
                
                out.close();
                out2.close();
                inFASTA.close();
                                
-               return 0;
+               return count;
        }
        catch(exception& e) {
                m->errorOut(e, "ChimeraPintailCommand", "driver");
@@ -513,7 +596,7 @@ int ChimeraPintailCommand::driver(linePair* line, string outputFName, string fil
 }
 //**********************************************************************************************************************
 #ifdef USE_MPI
-int ChimeraPintailCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& outMPI, MPI_File& outAccMPI, vector<long>& MPIPos){
+int ChimeraPintailCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& outMPI, MPI_File& outAccMPI, vector<unsigned long int>& MPIPos){
        try {
                                
                MPI_Status status; 
@@ -535,7 +618,7 @@ int ChimeraPintailCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_Fi
                        istringstream iss (tempBuf,istringstream::in);
                        delete buf4;
 
-                       Sequence* candidateSeq = new Sequence(iss);  gobble(iss);
+                       Sequence* candidateSeq = new Sequence(iss);  m->gobble(iss);
                                
                        if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
                                
@@ -575,7 +658,7 @@ int ChimeraPintailCommand::createProcesses(string outputFileName, string filenam
        try {
 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
                int process = 0;
-               //              processIDS.resize(0);
+               int num = 0;
                
                //loop through and create all the processes you want
                while (process != processors) {
@@ -585,7 +668,15 @@ int ChimeraPintailCommand::createProcesses(string outputFileName, string filenam
                                processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
                                process++;
                        }else if (pid == 0){
-                               driver(lines[process], outputFileName + toString(getpid()) + ".temp", filename, accnos + toString(getpid()) + ".temp");
+                               num = driver(lines[process], outputFileName + toString(getpid()) + ".temp", filename, accnos + toString(getpid()) + ".temp");
+                               
+                               //pass numSeqs to parent
+                               ofstream out;
+                               string tempFile = outputFileName + toString(getpid()) + ".num.temp";
+                               m->openOutputFile(tempFile, out);
+                               out << num << endl;
+                               out.close();
+
                                exit(0);
                        }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); }
                }
@@ -596,7 +687,15 @@ int ChimeraPintailCommand::createProcesses(string outputFileName, string filenam
                        wait(&temp);
                }
                
-               return 0;
+               for (int i = 0; i < processIDS.size(); i++) {
+                       ifstream in;
+                       string tempFile =  outputFileName + toString(processIDS[i]) + ".num.temp";
+                       m->openInputFile(tempFile, in);
+                       if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; }
+                       in.close(); remove(tempFile.c_str());
+               }
+               
+               return num;
 #endif         
        }
        catch(exception& e) {