]> git.donarmstrong.com Git - mothur.git/blobdiff - chimeracheckcommand.cpp
changes while testing
[mothur.git] / chimeracheckcommand.cpp
index de6f438d81f7fcd62e111d97a02e87d63aad1e66..31a36f73c6d0339d775dd6a1a4efa8e523c0f14f 100644 (file)
  */
 
 #include "chimeracheckcommand.h"
+#include "referencedb.h"
 
-//***************************************************************************************************************
+//**********************************************************************************************************************
+vector<string> ChimeraCheckCommand::setParameters(){   
+       try {
+               CommandParameter ptemplate("reference", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(ptemplate);
+               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","chimera",false,true,true); parameters.push_back(pfasta);
+               CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none","",false,false,true); parameters.push_back(pname);
+               CommandParameter psvg("svg", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(psvg);
+               CommandParameter pincrement("increment", "Number", "", "10", "", "", "","",false,false); parameters.push_back(pincrement);
+               CommandParameter pksize("ksize", "Number", "", "7", "", "", "","",false,false); parameters.push_back(pksize);
+               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false,true); parameters.push_back(pprocessors);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+               CommandParameter psave("save", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(psave);
 
+               vector<string> myArray;
+               for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
+               return myArray;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraCheckCommand", "setParameters");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+string ChimeraCheckCommand::getHelpString(){   
+       try {
+               string helpString = "";
+               helpString += "The chimera.check command reads a fastafile and referencefile and outputs potentially chimeric sequences.\n";
+               helpString += "This command was created using the algorythms described in CHIMERA_CHECK version 2.7 written by Niels Larsen. \n";
+               helpString += "The chimera.check command parameters are fasta, reference, processors, ksize, increment, svg and name.\n";
+               helpString += "The fasta parameter allows you to enter the fasta file containing your potentially chimeric sequences, and is required unless you have a valid current fasta file. \n";
+               helpString += "You may enter multiple fasta files by separating their names with dashes. ie. fasta=abrecovery.fasta-amzon.fasta \n";
+               helpString += "The reference parameter allows you to enter a reference file containing known non-chimeric sequences, and is required. \n";
+               helpString += "The processors parameter allows you to specify how many processors you would like to use.  The default is 1. \n";
+#ifdef USE_MPI
+               helpString += "When using MPI, the processors parameter is set to the number of MPI processes running. \n";
+#endif
+               helpString += "The increment parameter allows you to specify how far you move each window while finding chimeric sequences, default is 10.\n";
+               helpString += "The ksize parameter allows you to input kmersize, default is 7. \n";
+               helpString += "The svg parameter allows you to specify whether or not you would like a svg file outputted for each query sequence, default is False.\n";
+               helpString += "The name parameter allows you to enter a file containing names of sequences you would like .svg files for.\n";
+               helpString += "You may enter multiple name files by separating their names with dashes. ie. fasta=abrecovery.svg.names-amzon.svg.names \n";
+               helpString += "If the save parameter is set to true the reference sequences will be saved in memory, to clear them later you can use the clear.memory command. Default=f.";
+               helpString += "The chimera.check command should be in the following format: \n";
+               helpString += "chimera.check(fasta=yourFastaFile, reference=yourTemplateFile, processors=yourProcessors, ksize=yourKmerSize) \n";
+               helpString += "Example: chimera.check(fasta=AD.fasta, reference=core_set_aligned,imputed.fasta, processors=4, ksize=8) \n";
+               helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n";       
+               return helpString;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraCheckCommand", "getHelpString");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+string ChimeraCheckCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "chimera") {  pattern = "[filename],chimeracheck.chimeras"; } 
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "ChimeraCheckCommand", "getOutputPattern");
+        exit(1);
+    }
+}
+//**********************************************************************************************************************
+ChimeraCheckCommand::ChimeraCheckCommand(){    
+       try {
+               abort = true; calledHelp = true;
+               setParameters();
+               vector<string> tempOutNames;
+               outputTypes["chimera"] = tempOutNames;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraCheckCommand", "ChimeraCheckCommand");
+               exit(1);
+       }
+}
+//***************************************************************************************************************
 ChimeraCheckCommand::ChimeraCheckCommand(string option)  {
        try {
-               abort = false;
+               abort = false; calledHelp = false;  
+               ReferenceDB* rdb = ReferenceDB::getInstance();
                
                //allow user to run help
-               if(option == "help") { help(); abort = true; }
+               if(option == "help") { help(); abort = true; calledHelp = true; }
+               else if(option == "citation") { citation(); abort = true; calledHelp = true;}
                
                else {
-                       //valid paramters for this command
-                       string Array[] =  {"fasta","processors","increment","template","ksize","svg", "name","outputdir","inputdir" };
-                       vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+                       vector<string> myArray = setParameters();
                        
                        OptionParser parser(option);
                        map<string,string> parameters = parser.getParameters();
@@ -34,54 +116,95 @@ ChimeraCheckCommand::ChimeraCheckCommand(string option)  {
                                if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
                        }
                        
+                       vector<string> tempOutNames;
+                       outputTypes["chimera"] = tempOutNames;
+               
                        //if the user changes the input directory command factory will send this info to us in the output parameter 
                        string inputDir = validParameter.validFile(parameters, "inputdir", false);              
                        if (inputDir == "not found"){   inputDir = "";          }
                        else {
-                               it = parameters.find("template");
+                               it = parameters.find("reference");
                                //user has given a template file
                                if(it != parameters.end()){ 
-                                       string path = hasPath(it->second);
+                                       string path = m->hasPath(it->second);
                                        //if the user has not given a path then, add inputdir. else leave path alone.
-                                       if (path == "") {       parameters["template"] = inputDir + it->second;         }
+                                       if (path == "") {       parameters["reference"] = inputDir + it->second;                }
                                }
                        }
                        
                        //check for required parameters
                        fastafile = validParameter.validFile(parameters, "fasta", false);
-                       if (fastafile == "not found") { fastafile = ""; m->mothurOut("fasta is a required parameter for the chimera.check command."); m->mothurOutEndLine(); abort = true;  }
-                       else { 
-                               splitAtDash(fastafile, fastaFileNames);
+                       if (fastafile == "not found") {                                 
+                               //if there is a current fasta file, use it
+                               string filename = m->getFastaFile(); 
+                               if (filename != "") { fastaFileNames.push_back(filename); m->mothurOut("Using " + filename + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
+                               else {  m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
+                       }else { 
+                               m->splitAtDash(fastafile, fastaFileNames);
                                
                                //go through files and make sure they are good, if not, then disregard them
                                for (int i = 0; i < fastaFileNames.size(); i++) {
-                                       if (inputDir != "") {
-                                               string path = hasPath(fastaFileNames[i]);
-                                               //if the user has not given a path then, add inputdir. else leave path alone.
-                                               if (path == "") {       fastaFileNames[i] = inputDir + fastaFileNames[i];               }
-                                       }
-       
-                                       int ableToOpen;
-                                       ifstream in;
                                        
-                                       ableToOpen = openInputFile(fastaFileNames[i], in, "noerror");
-                               
-                                       //if you can't open it, try default location
-                                       if (ableToOpen == 1) {
-                                               if (m->getDefaultPath() != "") { //default path is set
-                                                       string tryPath = m->getDefaultPath() + getSimpleName(fastaFileNames[i]);
-                                                       m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
-                                                       ableToOpen = openInputFile(tryPath, in, "noerror");
-                                                       fastaFileNames[i] = tryPath;
+                                       bool ignore = false;
+                                       if (fastaFileNames[i] == "current") { 
+                                               fastaFileNames[i] = m->getFastaFile(); 
+                                               if (fastaFileNames[i] != "") {  m->mothurOut("Using " + fastaFileNames[i] + " as input file for the fasta parameter where you had given current."); m->mothurOutEndLine(); }
+                                               else {  
+                                                       m->mothurOut("You have no current fastafile, ignoring current."); m->mothurOutEndLine(); ignore=true; 
+                                                       //erase from file list
+                                                       fastaFileNames.erase(fastaFileNames.begin()+i);
+                                                       i--;
                                                }
                                        }
-                                       in.close();
                                        
-                                       if (ableToOpen == 1) { 
-                                               m->mothurOut("Unable to open " + fastaFileNames[i] +". It will be disregarded."); m->mothurOutEndLine(); 
-                                               //erase from file list
-                                               fastaFileNames.erase(fastaFileNames.begin()+i);
-                                               i--;
+                                       if (!ignore) {
+                                       
+                                       
+                                               if (inputDir != "") {
+                                                       string path = m->hasPath(fastaFileNames[i]);
+                                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                                       if (path == "") {       fastaFileNames[i] = inputDir + fastaFileNames[i];               }
+                                               }
+               
+                                               int ableToOpen;
+                                               ifstream in;
+                                               
+                                               ableToOpen = m->openInputFile(fastaFileNames[i], in, "noerror");
+                                       
+                                               //if you can't open it, try default location
+                                               if (ableToOpen == 1) {
+                                                       if (m->getDefaultPath() != "") { //default path is set
+                                                               string tryPath = m->getDefaultPath() + m->getSimpleName(fastaFileNames[i]);
+                                                               m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
+                                                               ifstream in2;
+                                                               ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                               in2.close();
+                                                               fastaFileNames[i] = tryPath;
+                                                       }
+                                               }
+                                               
+                                               //if you can't open it, try default location
+                                               if (ableToOpen == 1) {
+                                                       if (m->getOutputDir() != "") { //default path is set
+                                                               string tryPath = m->getOutputDir() + m->getSimpleName(fastaFileNames[i]);
+                                                               m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+                                                               ifstream in2;
+                                                               ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                               in2.close();
+                                                               fastaFileNames[i] = tryPath;
+                                                       }
+                                               }
+                                               
+                                               in.close();
+                                               
+                                               if (ableToOpen == 1) { 
+                                                       m->mothurOut("Unable to open " + fastaFileNames[i] +". It will be disregarded."); m->mothurOutEndLine(); 
+                                                       //erase from file list
+                                                       fastaFileNames.erase(fastaFileNames.begin()+i);
+                                                       i--;
+                                               }else {
+                                                       m->setFastaFile(fastaFileNames[i]);
+                                               }
                                        }
                                }
                                
@@ -91,47 +214,75 @@ ChimeraCheckCommand::ChimeraCheckCommand(string option)  {
                        
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
                        outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
-
-                       templatefile = validParameter.validFile(parameters, "template", true);
-                       if (templatefile == "not open") { abort = true; }
-                       else if (templatefile == "not found") { templatefile = "";  m->mothurOut("template is a required parameter for the chimera.check command."); m->mothurOutEndLine(); abort = true;  }    
                        
                        namefile = validParameter.validFile(parameters, "name", false);
                        if (namefile == "not found") { namefile = ""; }
                        else { 
-                               splitAtDash(namefile, nameFileNames);
+                               m->splitAtDash(namefile, nameFileNames);
                                
                                //go through files and make sure they are good, if not, then disregard them
                                for (int i = 0; i < nameFileNames.size(); i++) {
-                                       if (inputDir != "") {
-                                               string path = hasPath(nameFileNames[i]);
-                                               //if the user has not given a path then, add inputdir. else leave path alone.
-                                               if (path == "") {       nameFileNames[i] = inputDir + nameFileNames[i];         }
-                                       }
-       
-                                       int ableToOpen;
-                                       ifstream in;
                                        
-                                       ableToOpen = openInputFile(nameFileNames[i], in, "noerror");
-                               
-                                       //if you can't open it, try default location
-                                       if (ableToOpen == 1) {
-                                               if (m->getDefaultPath() != "") { //default path is set
-                                                       string tryPath = m->getDefaultPath() + getSimpleName(nameFileNames[i]);
-                                                       m->mothurOut("Unable to open " + nameFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
-                                                       ableToOpen = openInputFile(tryPath, in, "noerror");
-                                                       nameFileNames[i] = tryPath;
+                                       bool ignore = false;
+                                       if (nameFileNames[i] == "current") { 
+                                               nameFileNames[i] = m->getNameFile(); 
+                                               if (nameFileNames[i] != "") {  m->mothurOut("Using " + nameFileNames[i] + " as input file for the name parameter where you had given current."); m->mothurOutEndLine(); }
+                                               else {  
+                                                       m->mothurOut("You have no current namefile, ignoring current."); m->mothurOutEndLine(); ignore=true; 
+                                                       //erase from file list
+                                                       nameFileNames.erase(nameFileNames.begin()+i);
+                                                       i--;
                                                }
                                        }
-                                       in.close();
                                        
-                                       if (ableToOpen == 1) { 
-                                               m->mothurOut("Unable to open " + nameFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine(); 
-                                               //erase from file list
-                                               nameFileNames.erase(nameFileNames.begin()+i);
-                                               i--;
-                                       }
+                                       if (!ignore) {
                                        
+                                               if (inputDir != "") {
+                                                       string path = m->hasPath(nameFileNames[i]);
+                                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                                       if (path == "") {       nameFileNames[i] = inputDir + nameFileNames[i];         }
+                                               }
+               
+                                               int ableToOpen;
+                                               ifstream in;
+                                               
+                                               ableToOpen = m->openInputFile(nameFileNames[i], in, "noerror");
+                                       
+                                               //if you can't open it, try default location
+                                               if (ableToOpen == 1) {
+                                                       if (m->getDefaultPath() != "") { //default path is set
+                                                               string tryPath = m->getDefaultPath() + m->getSimpleName(nameFileNames[i]);
+                                                               m->mothurOut("Unable to open " + nameFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
+                                                               ifstream in2;
+                                                               ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                               in2.close();
+                                                               nameFileNames[i] = tryPath;
+                                                       }
+                                               }
+                                               
+                                               //if you can't open it, try default location
+                                               if (ableToOpen == 1) {
+                                                       if (m->getOutputDir() != "") { //default path is set
+                                                               string tryPath = m->getOutputDir() + m->getSimpleName(nameFileNames[i]);
+                                                               m->mothurOut("Unable to open " + nameFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+                                                               ifstream in2;
+                                                               ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+                                                               in2.close();
+                                                               nameFileNames[i] = tryPath;
+                                                       }
+                                               }
+                                               
+                                               in.close();
+                                               
+                                               if (ableToOpen == 1) { 
+                                                       m->mothurOut("Unable to open " + nameFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine(); 
+                                                       //erase from file list
+                                                       nameFileNames.erase(nameFileNames.begin()+i);
+                                                       i--;
+                                               }else {
+                                                       m->setNameFile(nameFileNames[i]);
+                                               }
+                                       }
                                }
                                
                                //make sure there is at least one valid file left
@@ -143,18 +294,41 @@ ChimeraCheckCommand::ChimeraCheckCommand(string option)  {
                                }
                        }
 
-                       string temp = validParameter.validFile(parameters, "processors", false);                if (temp == "not found") { temp = "1"; }
-                       convert(temp, processors);
+                       string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = m->getProcessors();      }
+                       m->setProcessors(temp);
+                       m->mothurConvert(temp, processors);
+                       
+                       temp = validParameter.validFile(parameters, "save", false);                     if (temp == "not found"){       temp = "f";                             }
+                       save = m->isTrue(temp); 
+                       rdb->save = save; 
+                       if (save) { //clear out old references
+                               rdb->clearMemory();     
+                       }
+                       
+                       //this has to go after save so that if the user sets save=t and provides no reference we abort
+                       templatefile = validParameter.validFile(parameters, "reference", true);
+                       if (templatefile == "not found") { 
+                               //check for saved reference sequences
+                               if (rdb->referenceSeqs.size() != 0) {
+                                       templatefile = "saved";
+                               }else {
+                                       m->mothurOut("[ERROR]: You don't have any saved reference sequences and the reference parameter is a required."); 
+                                       m->mothurOutEndLine();
+                                       abort = true; 
+                               }
+                       }else if (templatefile == "not open") { abort = true; } 
+                       else {  if (save) {     rdb->setSavedReference(templatefile);   }       }
+                       
                        
                        temp = validParameter.validFile(parameters, "ksize", false);                    if (temp == "not found") { temp = "7"; }
-                       convert(temp, ksize);
+                       m->mothurConvert(temp, ksize);
                        
                        temp = validParameter.validFile(parameters, "svg", false);                              if (temp == "not found") { temp = "F"; }
-                       svg = isTrue(temp);
+                       svg = m->isTrue(temp);
                        if (nameFileNames.size() != 0) { svg = true; }
                        
                        temp = validParameter.validFile(parameters, "increment", false);                if (temp == "not found") { temp = "10"; }
-                       convert(temp, increment);                       
+                       m->mothurConvert(temp, increment);                      
                }
        }
        catch(exception& e) {
@@ -162,47 +336,12 @@ ChimeraCheckCommand::ChimeraCheckCommand(string option)  {
                exit(1);
        }
 }
-//**********************************************************************************************************************
-
-void ChimeraCheckCommand::help(){
-       try {
-       
-               m->mothurOut("The chimera.check command reads a fastafile and templatefile and outputs potentially chimeric sequences.\n");
-               m->mothurOut("This command was created using the algorythms described in CHIMERA_CHECK version 2.7 written by Niels Larsen. \n");
-               m->mothurOut("The chimera.check command parameters are fasta, template, processors, ksize, increment, svg and name.\n");
-               m->mothurOut("The fasta parameter allows you to enter the fasta file containing your potentially chimeric sequences, and is required. \n");
-               m->mothurOut("You may enter multiple fasta files by separating their names with dashes. ie. fasta=abrecovery.fasta-amzon.fasta \n");
-               m->mothurOut("The template parameter allows you to enter a template file containing known non-chimeric sequences, and is required. \n");
-               m->mothurOut("The processors parameter allows you to specify how many processors you would like to use.  The default is 1. \n");
-               #ifdef USE_MPI
-               m->mothurOut("When using MPI, the processors parameter is set to the number of MPI processes running. \n");
-               #endif
-               m->mothurOut("The increment parameter allows you to specify how far you move each window while finding chimeric sequences, default is 10.\n");
-               m->mothurOut("The ksize parameter allows you to input kmersize, default is 7. \n");
-               m->mothurOut("The svg parameter allows you to specify whether or not you would like a svg file outputted for each query sequence, default is False.\n");
-               m->mothurOut("The name parameter allows you to enter a file containing names of sequences you would like .svg files for.\n");
-               m->mothurOut("You may enter multiple name files by separating their names with dashes. ie. fasta=abrecovery.svg.names-amzon.svg.names \n");
-               m->mothurOut("The chimera.check command should be in the following format: \n");
-               m->mothurOut("chimera.check(fasta=yourFastaFile, template=yourTemplateFile, processors=yourProcessors, ksize=yourKmerSize) \n");
-               m->mothurOut("Example: chimera.check(fasta=AD.fasta, template=core_set_aligned,imputed.fasta, processors=4, ksize=8) \n");
-               m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");     
-       }
-       catch(exception& e) {
-               m->errorOut(e, "ChimeraCheckCommand", "help");
-               exit(1);
-       }
-}
-
-//***************************************************************************************************************
-
-ChimeraCheckCommand::~ChimeraCheckCommand(){   /*      do nothing      */      }
-
 //***************************************************************************************************************
 
 int ChimeraCheckCommand::execute(){
        try{
                
-               if (abort == true) { return 0; }
+               if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
                for (int i = 0; i < fastaFileNames.size(); i++) {
                                
@@ -217,14 +356,17 @@ int ChimeraCheckCommand::execute(){
 
                        if (m->control_pressed) { delete chimera;       return 0;       }
                        
-                       string outputFileName = outputDir + getRootName(getSimpleName(fastaFileNames[i]))  + "chimeracheck.chimeras";
-                       outputNames.push_back(outputFileName);
+                       if (outputDir == "") { outputDir = m->hasPath(fastaFileNames[i]);  }//if user entered a file with a path then preserve it
+            map<string, string> variables;
+            variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[i]));
+                       string outputFileName = getOutputFileName("chimera", variables);
+                       outputNames.push_back(outputFileName); outputTypes["chimera"].push_back(outputFileName);
                        
                #ifdef USE_MPI
                
-                               int pid, end, numSeqsPerProcessor; 
+                               int pid, numSeqsPerProcessor; 
                                int tag = 2001;
-                               vector<unsigned long int> MPIPos;
+                               vector<unsigned long long> MPIPos;
                                
                                MPI_Status status; 
                                MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
@@ -245,10 +387,10 @@ int ChimeraCheckCommand::execute(){
                                MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI);  //comm, filename, mode, info, filepointer
                                MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI);
                                
-                               if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);  for (int j = 0; j < outputNames.size(); j++) {    remove(outputNames[j].c_str()); } delete chimera; return 0;  }
+                               if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);  for (int j = 0; j < outputNames.size(); j++) {    m->mothurRemove(outputNames[j]);        } outputTypes.clear(); delete chimera; return 0;  }
                                
                                if (pid == 0) { //you are the root process 
-                                       MPIPos = setFilePosFasta(fastaFileNames[i], numSeqs); //fills MPIPos, returns numSeqs
+                                       MPIPos = m->setFilePosFasta(fastaFileNames[i], numSeqs); //fills MPIPos, returns numSeqs
                                        
                                        //send file positions to all processes
                                        for(int j = 1; j < processors; j++) { 
@@ -265,12 +407,12 @@ int ChimeraCheckCommand::execute(){
                                        //align your part
                                        driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, MPIPos);
                                        
-                                       if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);  for (int j = 0; j < outputNames.size(); j++) {    remove(outputNames[j].c_str()); }   delete chimera; return 0;  }
+                                       if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);  for (int j = 0; j < outputNames.size(); j++) {    m->mothurRemove(outputNames[j]);        }   outputTypes.clear(); delete chimera; return 0;  }
                                        
                                        //wait on chidren
                                        for(int j = 1; j < processors; j++) { 
-                                               char buf[4];
-                                               MPI_Recv(buf, 4, MPI_CHAR, j, tag, MPI_COMM_WORLD, &status); 
+                                               char buf[5];
+                                               MPI_Recv(buf, 5, MPI_CHAR, j, tag, MPI_COMM_WORLD, &status); 
                                        }
                                }else{ //you are a child process
                                        MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
@@ -285,12 +427,12 @@ int ChimeraCheckCommand::execute(){
                                        //align your part
                                        driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, MPIPos);
                                        
-                                       if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
+                                       if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   for (int j = 0; j < outputNames.size(); j++) {   m->mothurRemove(outputNames[j]);        }  outputTypes.clear(); delete chimera; return 0;  }
                                        
                                        //tell parent you are done.
-                                       char buf[4];
+                                       char buf[5];
                                        strcpy(buf, "done"); 
-                                       MPI_Send(buf, 4, MPI_CHAR, 0, tag, MPI_COMM_WORLD);
+                                       MPI_Send(buf, 5, MPI_CHAR, 0, tag, MPI_COMM_WORLD);
                                }
                                
                                //close files 
@@ -299,18 +441,20 @@ int ChimeraCheckCommand::execute(){
                                MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
                #else
                        
-                       vector<unsigned long int> positions = divideFile(fastaFileNames[i], processors);
-                               
-                       for (int s = 0; s < (positions.size()-1); s++) {
-                               lines.push_back(new linePair(positions[s], positions[(s+1)]));
-                       }       
+                       
                        
                        //break up file
-                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+                               vector<unsigned long long> positions = m->divideFile(fastaFileNames[i], processors);
+                       
+                               for (int s = 0; s < (positions.size()-1); s++) {
+                                       lines.push_back(new linePair(positions[s], positions[(s+1)]));
+                               }       
+                       
                                if(processors == 1){
                                        numSeqs = driver(lines[0], outputFileName, fastaFileNames[i]);
                                        
-                                       if (m->control_pressed) { for (int j = 0; j < outputNames.size(); j++) {        remove(outputNames[j].c_str()); } for (int j = 0; j < lines.size(); j++) {  delete lines[j];  }  lines.clear(); delete chimera; return 0; }
+                                       if (m->control_pressed) { for (int j = 0; j < outputNames.size(); j++) {        m->mothurRemove(outputNames[j]);        } for (int j = 0; j < lines.size(); j++) {  delete lines[j];  } outputTypes.clear();  lines.clear(); delete chimera; return 0; }
                                                                        
                                }else{
                                        processIDS.resize(0);
@@ -321,12 +465,12 @@ int ChimeraCheckCommand::execute(){
                                                
                                        //append output files
                                        for(int j=1;j<processors;j++){
-                                               appendFiles((outputFileName + toString(processIDS[j]) + ".temp"), outputFileName);
-                                               remove((outputFileName + toString(processIDS[j]) + ".temp").c_str());
+                                               m->appendFiles((outputFileName + toString(processIDS[j]) + ".temp"), outputFileName);
+                                               m->mothurRemove((outputFileName + toString(processIDS[j]) + ".temp"));
                                        }
                                        
                                        if (m->control_pressed) { 
-                                               for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } 
+                                               for (int j = 0; j < outputNames.size(); j++) {  m->mothurRemove(outputNames[j]);        } outputTypes.clear();
                                                for (int j = 0; j < lines.size(); j++) {  delete lines[j];  }  lines.clear();
                                                delete chimera;
                                                return 0;
@@ -334,9 +478,10 @@ int ChimeraCheckCommand::execute(){
                                }
 
                        #else
+                               lines.push_back(new linePair(0, 1000));
                                numSeqs = driver(lines[0], outputFileName, fastaFileNames[i]);
                                
-                               if (m->control_pressed) { for (int j = 0; j < lines.size(); j++) {  delete lines[j];  }  lines.clear(); for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } delete chimera; return 0; }
+                               if (m->control_pressed) { for (int j = 0; j < lines.size(); j++) {  delete lines[j];  }  lines.clear(); for (int j = 0; j < outputNames.size(); j++) {  m->mothurRemove(outputNames[j]);        } outputTypes.clear(); delete chimera; return 0; }
                        #endif
                #endif          
                        delete chimera;
@@ -365,12 +510,12 @@ int ChimeraCheckCommand::execute(){
 int ChimeraCheckCommand::driver(linePair* filePos, string outputFName, string filename){
        try {
                ofstream out;
-               openOutputFile(outputFName, out);
+               m->openOutputFile(outputFName, out);
                
                ofstream out2;
                
                ifstream inFASTA;
-               openInputFile(filename, inFASTA);
+               m->openInputFile(filename, inFASTA);
 
                inFASTA.seekg(filePos->start);
 
@@ -381,7 +526,7 @@ int ChimeraCheckCommand::driver(linePair* filePos, string outputFName, string fi
 
                        if (m->control_pressed) {       return 1;       }
                
-                       Sequence* candidateSeq = new Sequence(inFASTA);  gobble(inFASTA);
+                       Sequence* candidateSeq = new Sequence(inFASTA);  m->gobble(inFASTA);
                                
                        if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
                                //find chimeras
@@ -394,14 +539,18 @@ int ChimeraCheckCommand::driver(linePair* filePos, string outputFName, string fi
                        }
                        delete candidateSeq;
                        
-                       unsigned long int pos = inFASTA.tellg();
-                       if ((pos == -1) || (pos >= filePos->end)) { break; }
+                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+                               unsigned long long pos = inFASTA.tellg();
+                               if ((pos == -1) || (pos >= filePos->end)) { break; }
+                       #else
+                               if (inFASTA.eof()) { break; }
+                       #endif
                        
                        //report progress
-                       if((count) % 100 == 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
+                       if((count) % 100 == 0){ m->mothurOutJustToScreen("Processing sequence: " + toString(count) + "\n");             }
                }
                //report progress
-               if((count) % 100 != 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
+               if((count) % 100 != 0){ m->mothurOutJustToScreen("Processing sequence: " + toString(count) + "\n");     }
                
                out.close();
                inFASTA.close();
@@ -415,7 +564,7 @@ int ChimeraCheckCommand::driver(linePair* filePos, string outputFName, string fi
 }
 //**********************************************************************************************************************
 #ifdef USE_MPI
-int ChimeraCheckCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& outMPI, vector<unsigned long int>& MPIPos){
+int ChimeraCheckCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& outMPI, vector<unsigned long long>& MPIPos){
        try {
                MPI_File outAccMPI;
                MPI_Status status; 
@@ -437,7 +586,7 @@ int ChimeraCheckCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File
                        istringstream iss (tempBuf,istringstream::in);
                        delete buf4;
 
-                       Sequence* candidateSeq = new Sequence(iss);  gobble(iss);
+                       Sequence* candidateSeq = new Sequence(iss);  m->gobble(iss);
                                
                        if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
                                //find chimeras
@@ -449,10 +598,10 @@ int ChimeraCheckCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File
                        delete candidateSeq;
                        
                        //report progress
-                       if((i+1) % 100 == 0){  cout << "Processing sequence: " << (i+1) << endl;        m->mothurOutJustToLog("Processing sequence: " + toString(i+1) + "\n");          }
+                       if((i+1) % 100 == 0){  cout << "Processing sequence: " << (i+1) << endl;                }
                }
                //report progress
-               if(num % 100 != 0){             cout << "Processing sequence: " << num << endl; m->mothurOutJustToLog("Processing sequence: " + toString(num) + "\n");  }
+               if(num % 100 != 0){             cout << "Processing sequence: " << num << endl;         }
                
                return 0;
        }
@@ -467,7 +616,7 @@ int ChimeraCheckCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File
 
 int ChimeraCheckCommand::createProcesses(string outputFileName, string filename) {
        try {
-#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
                int process = 0;
                int num = 0;
                
@@ -484,12 +633,16 @@ int ChimeraCheckCommand::createProcesses(string outputFileName, string filename)
                                //pass numSeqs to parent
                                ofstream out;
                                string tempFile = outputFileName + toString(getpid()) + ".num.temp";
-                               openOutputFile(tempFile, out);
+                               m->openOutputFile(tempFile, out);
                                out << num << endl;
                                out.close();
                                
                                exit(0);
-                       }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); }
+                       }else { 
+                               m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); 
+                               for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+                               exit(0);
+                       }
                }
                
                //force parent to wait until all the processes are done
@@ -501,9 +654,9 @@ int ChimeraCheckCommand::createProcesses(string outputFileName, string filename)
                for (int i = 0; i < processIDS.size(); i++) {
                        ifstream in;
                        string tempFile =  outputFileName + toString(processIDS[i]) + ".num.temp";
-                       openInputFile(tempFile, in);
+                       m->openInputFile(tempFile, in);
                        if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; }
-                       in.close(); remove(tempFile.c_str());
+                       in.close(); m->mothurRemove(tempFile);
                }
                
                return num;