/**************************************************************************************************/
-AlignmentDB::AlignmentDB(string fastaFileName, string method, int kmerSize, int gapOpen, int gapExtend, int match, int misMatch){ // This assumes that the template database is in fasta format, may
+AlignmentDB::AlignmentDB(string fastaFileName, string s, int kmerSize, float gapOpen, float gapExtend, float match, float misMatch){ // This assumes that the template database is in fasta format, may
try { // need to alter this in the future?
+ m = MothurOut::getInstance();
longest = 0;
+ method = s;
+ bool needToGenerate = true;
- ifstream fastaFile;
- openInputFile(fastaFileName, fastaFile);
+ m->mothurOutEndLine();
+ m->mothurOut("Reading in the " + fastaFileName + " template sequences...\t"); cout.flush();
- mothurOutEndLine();
- mothurOut("Reading in the " + fastaFileName + " template sequences...\t"); cout.flush();
+ #ifdef USE_MPI
+ int pid, processors;
+ vector<unsigned long int> positions;
- while (!fastaFile.eof()) {
- Sequence temp(fastaFile);
-
- templateSequences.push_back(temp);
+ MPI_Status status;
+ MPI_File inMPI;
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+ MPI_Comm_size(MPI_COMM_WORLD, &processors);
+ int tag = 2001;
+
+ char inFileName[1024];
+ strcpy(inFileName, fastaFileName.c_str());
+
+ MPI_File_open(MPI_COMM_WORLD, inFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
- //save longest base
- if (temp.getUnaligned().length() > longest) { longest = temp.getUnaligned().length(); }
+ if (pid == 0) {
+ positions = m->setFilePosFasta(fastaFileName, numSeqs); //fills MPIPos, returns numSeqs
+
+ //send file positions to all processes
+ for(int i = 1; i < processors; i++) {
+ MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
+ MPI_Send(&positions[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
+ }
+ }else{
+ MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
+ positions.resize(numSeqs+1);
+ MPI_Recv(&positions[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
+ }
+
+ //read file
+ for(int i=0;i<numSeqs;i++){
+
+ if (m->control_pressed) { templateSequences.clear(); break; }
+
+ //read next sequence
+ int length = positions[i+1] - positions[i];
+ char* buf4 = new char[length];
- gobble(fastaFile);
- }
+ MPI_File_read_at(inMPI, positions[i], buf4, length, MPI_CHAR, &status);
- numSeqs = templateSequences.size();
+ string tempBuf = buf4;
+ if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
+ delete buf4;
+
+ istringstream iss (tempBuf,istringstream::in);
+ Sequence temp(iss);
+ if (temp.getName() != "") {
+ templateSequences.push_back(temp);
+ //save longest base
+ if (temp.getUnaligned().length() >= longest) { longest = temp.getUnaligned().length()+1; }
+ }
+ }
+
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
+
+ MPI_File_close(&inMPI);
+
+ #else
+ ifstream fastaFile;
+ m->openInputFile(fastaFileName, fastaFile);
+
+ while (!fastaFile.eof()) {
+ Sequence temp(fastaFile); m->gobble(fastaFile);
+
+ if (m->control_pressed) { templateSequences.clear(); break; }
+
+ if (temp.getName() != "") {
+ templateSequences.push_back(temp);
+ //save longest base
+ if (temp.getUnaligned().length() >= longest) { longest = (temp.getUnaligned().length()+1); }
+ }
+ }
fastaFile.close();
+ #endif
+
+ numSeqs = templateSequences.size();
//all of this is elsewhere already!
- mothurOut("DONE.");
- mothurOutEndLine(); cout.flush();
+ m->mothurOut("DONE.");
+ m->mothurOutEndLine(); cout.flush();
//in case you delete the seqs and then ask for them
emptySequence = Sequence();
emptySequence.setUnaligned("XXXXXXXXXXXXXXXXXXXXXXXXXXXXX");
emptySequence.setAligned("XXXXXXXXXXXXXXXXXXXXXXXXXXXXX");
- bool needToGenerate = true;
+
string kmerDBName;
if(method == "kmer") {
search = new KmerDB(fastaFileName, kmerSize);
- kmerDBName = fastaFileName.substr(0,fastaFileName.find_last_of(".")+1) + char('0'+ kmerSize) + "mer";
- ifstream kmerFileTest(kmerDBName.c_str());
-
- if(kmerFileTest){ needToGenerate = false; }
+ #ifdef USE_MPI
+ #else
+ kmerDBName = fastaFileName.substr(0,fastaFileName.find_last_of(".")+1) + char('0'+ kmerSize) + "mer";
+ ifstream kmerFileTest(kmerDBName.c_str());
+
+ if(kmerFileTest){
+ bool GoodFile = m->checkReleaseVersion(kmerFileTest, m->getVersion());
+ if (GoodFile) { needToGenerate = false; }
+ }
+ #endif
}
else if(method == "suffix") { search = new SuffixDB(numSeqs); }
- else if(method == "blast") { search = new BlastDB(gapOpen, gapExtend, match, misMatch); }
+ else if(method == "blast") { search = new BlastDB(fastaFileName.substr(0,fastaFileName.find_last_of(".")+1), gapOpen, gapExtend, match, misMatch); }
else {
- mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8.");
- mothurOutEndLine();
+ m->mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8.");
+ m->mothurOutEndLine();
search = new KmerDB(fastaFileName, 8);
}
- if (needToGenerate) {
-
- //add sequences to search
- for (int i = 0; i < templateSequences.size(); i++) {
- search->addSequence(templateSequences[i]);
+ if (!(m->control_pressed)) {
+ if (needToGenerate) {
+ //add sequences to search
+ for (int i = 0; i < templateSequences.size(); i++) {
+ search->addSequence(templateSequences[i]);
+
+ if (m->control_pressed) { templateSequences.clear(); break; }
+ }
+
+ if (m->control_pressed) { templateSequences.clear(); }
+
+ search->generateDB();
+
+ }else if ((method == "kmer") && (!needToGenerate)) {
+ ifstream kmerFileTest(kmerDBName.c_str());
+ search->readKmerDB(kmerFileTest);
}
- search->generateDB();
-
- }else if ((method == "kmer") && (!needToGenerate)) {
- ifstream kmerFileTest(kmerDBName.c_str());
- search->readKmerDB(kmerFileTest);
+
+ search->setNumSeqs(numSeqs);
}
+ }
+ catch(exception& e) {
+ m->errorOut(e, "AlignmentDB", "AlignmentDB");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
+AlignmentDB::AlignmentDB(string s){
+ try {
+ m = MothurOut::getInstance();
+ method = s;
+
+ if(method == "suffix") { search = new SuffixDB(); }
+ else if(method == "blast") { search = new BlastDB(); }
+ else { search = new KmerDB(); }
+
+
+ //in case you delete the seqs and then ask for them
+ emptySequence = Sequence();
+ emptySequence.setName("no_match");
+ emptySequence.setUnaligned("XXXXXXXXXXXXXXXXXXXXXXXXXXXXX");
+ emptySequence.setAligned("XXXXXXXXXXXXXXXXXXXXXXXXXXXXX");
- search->setNumSeqs(numSeqs);
}
catch(exception& e) {
- errorOut(e, "AlignmentDB", "AlignmentDB");
+ m->errorOut(e, "AlignmentDB", "AlignmentDB");
exit(1);
}
}
}
catch(exception& e) {
- errorOut(e, "AlignmentDB", "findClosestSequence");
+ m->errorOut(e, "AlignmentDB", "findClosestSequence");
exit(1);
}
}
+
+
projects / mothur.git / blobdiff