seqsummarycommand.cpp

   1 /*
   2  *  seqcoordcommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by Pat Schloss on 5/30/09.
   6  *  Copyright 2009 Patrick D. Schloss. All rights reserved.
   7  *
   8  */
   9
  10 #include "seqsummarycommand.h"
  11 #include "sequence.hpp"
  12
  13 //**********************************************************************************************************************
  14 vector<string> SeqSummaryCommand::setParameters(){
  15         try {
  16                 CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfasta);
  17                 CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
  18                 CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors);
  19                 CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
  20                 CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
  21
  22                 vector<string> myArray;
  23                 for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
  24                 return myArray;
  25         }
  26         catch(exception& e) {
  27                 m->errorOut(e, "SeqSummaryCommand", "setParameters");
  28                 exit(1);
  29         }
  30 }
  31 //**********************************************************************************************************************
  32 string SeqSummaryCommand::getHelpString(){
  33         try {
  34                 string helpString = "";
  35                 helpString += "The summary.seqs command reads a fastafile and summarizes the sequences.\n";
  36                 helpString += "The summary.seqs command parameters are fasta, name and processors, fasta is required, unless you have a valid current fasta file.\n";
  37                 helpString += "The name parameter allows you to enter a name file associated with your fasta file. \n";
  38                 helpString += "The summary.seqs command should be in the following format: \n";
  39                 helpString += "summary.seqs(fasta=yourFastaFile, processors=2) \n";
  40                 helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n";
  41                 return helpString;
  42         }
  43         catch(exception& e) {
  44                 m->errorOut(e, "SeqSummaryCommand", "getHelpString");
  45                 exit(1);
  46         }
  47 }
  48
  49 //**********************************************************************************************************************
  50 SeqSummaryCommand::SeqSummaryCommand(){
  51         try {
  52                 abort = true; calledHelp = true;
  53                 setParameters();
  54                 vector<string> tempOutNames;
  55                 outputTypes["summary"] = tempOutNames;
  56         }
  57         catch(exception& e) {
  58                 m->errorOut(e, "SeqSummaryCommand", "SeqSummaryCommand");
  59                 exit(1);
  60         }
  61 }
  62 //***************************************************************************************************************
  63
  64 SeqSummaryCommand::SeqSummaryCommand(string option)  {
  65         try {
  66                 abort = false; calledHelp = false;
  67
  68                 //allow user to run help
  69                 if(option == "help") { help(); abort = true; calledHelp = true; }
  70                 else if(option == "citation") { citation(); abort = true; calledHelp = true;}
  71
  72                 else {
  73                         vector<string> myArray = setParameters();
  74
  75                         OptionParser parser(option);
  76                         map<string,string> parameters = parser.getParameters();
  77
  78                         ValidParameters validParameter("summary.seqs");
  79                         map<string,string>::iterator it;
  80
  81                         //check to make sure all parameters are valid for command
  82                         for (it = parameters.begin(); it != parameters.end(); it++) {
  83                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  84                         }
  85
  86                         //if the user changes the input directory command factory will send this info to us in the output parameter
  87                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  88                         if (inputDir == "not found"){   inputDir = "";          }
  89                         else {
  90                                 string path;
  91                                 it = parameters.find("fasta");
  92                                 //user has given a template file
  93                                 if(it != parameters.end()){
  94                                         path = m->hasPath(it->second);
  95                                         //if the user has not given a path then, add inputdir. else leave path alone.
  96                                         if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
  97                                 }
  98
  99                                 it = parameters.find("name");
 100                                 //user has given a template file
 101                                 if(it != parameters.end()){
 102                                         path = m->hasPath(it->second);
 103                                         //if the user has not given a path then, add inputdir. else leave path alone.
 104                                         if (path == "") {       parameters["name"] = inputDir + it->second;             }
 105                                 }
 106                         }
 107
 108                         //initialize outputTypes
 109                         vector<string> tempOutNames;
 110                         outputTypes["summary"] = tempOutNames;
 111
 112                         //check for required parameters
 113                         fastafile = validParameter.validFile(parameters, "fasta", true);
 114                         if (fastafile == "not open") { abort = true; }
 115                         else if (fastafile == "not found") {
 116                                 fastafile = m->getFastaFile();
 117                                 if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
 118                                 else {  m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
 119                         }
 120
 121                         namefile = validParameter.validFile(parameters, "name", true);
 122                         if (namefile == "not open") { namefile = ""; abort = true; }
 123                         else if (namefile == "not found") { namefile = "";  }
 124
 125                         //if the user changes the output directory command factory will send this info to us in the output parameter
 126                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){
 127                                 outputDir = "";
 128                                 outputDir += m->hasPath(fastafile); //if user entered a file with a path then preserve it
 129                         }
 130
 131                         string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = m->getProcessors();      }
 132                         m->setProcessors(temp);
 133                         convert(temp, processors);
 134
 135
 136                 }
 137         }
 138         catch(exception& e) {
 139                 m->errorOut(e, "SeqSummaryCommand", "SeqSummaryCommand");
 140                 exit(1);
 141         }
 142 }
 143 //***************************************************************************************************************
 144
 145 int SeqSummaryCommand::execute(){
 146         try{
 147
 148                 if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
 149
 150                 //set current fasta to fastafile
 151                 m->setFastaFile(fastafile);
 152
 153                 string summaryFile = outputDir + m->getSimpleName(fastafile) + ".summary";
 154
 155                 int numSeqs = 0;
 156
 157                 vector<int> startPosition;
 158                 vector<int> endPosition;
 159                 vector<int> seqLength;
 160                 vector<int> ambigBases;
 161                 vector<int> longHomoPolymer;
 162
 163                 if (namefile != "") { nameMap = m->readNames(namefile); }
 164
 165                 if (m->control_pressed) { return 0; }
 166
 167 #ifdef USE_MPI
 168                                 int pid, numSeqsPerProcessor;
 169                                 int tag = 2001;
 170                                 int startTag = 1; int endTag = 2; int lengthTag = 3; int baseTag = 4; int lhomoTag = 5;
 171                                 int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY;
 172                                 vector<unsigned long int> MPIPos;
 173
 174                                 MPI_Status status;
 175                                 MPI_Status statusOut;
 176                                 MPI_File inMPI;
 177                                 MPI_File outMPI;
 178                                 MPI_Comm_size(MPI_COMM_WORLD, &processors);
 179                                 MPI_Comm_rank(MPI_COMM_WORLD, &pid);
 180
 181                                 char tempFileName[1024];
 182                                 strcpy(tempFileName, fastafile.c_str());
 183
 184                                 char sumFileName[1024];
 185                                 strcpy(sumFileName, summaryFile.c_str());
 186
 187                                 MPI_File_open(MPI_COMM_WORLD, tempFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI);  //comm, filename, mode, info, filepointer
 188                                 MPI_File_open(MPI_COMM_WORLD, sumFileName, outMode, MPI_INFO_NULL, &outMPI);
 189
 190                                 if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI); return 0;  }
 191
 192                                 if (pid == 0) { //you are the root process
 193                                                 //print header
 194                                                 string outputString = "seqname\tstart\tend\tnbases\tambigs\tpolymer\tnumSeqs\n";
 195                                                 int length = outputString.length();
 196                                                 char* buf2 = new char[length];
 197                                                 memcpy(buf2, outputString.c_str(), length);
 198
 199                                                 MPI_File_write_shared(outMPI, buf2, length, MPI_CHAR, &statusOut);
 200                                                 delete buf2;
 201
 202                                                 MPIPos = m->setFilePosFasta(fastafile, numSeqs); //fills MPIPos, returns numSeqs
 203
 204                                                 for(int i = 1; i < processors; i++) {
 205                                                         MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
 206                                                         MPI_Send(&MPIPos[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
 207                                                 }
 208
 209                                                 //figure out how many sequences you have to do
 210                                                 numSeqsPerProcessor = numSeqs / processors;
 211                                                 int startIndex =  pid * numSeqsPerProcessor;
 212                                                 if(pid == (processors - 1)){    numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor;      }
 213
 214                                                 //do your part
 215                                                 MPICreateSummary(startIndex, numSeqsPerProcessor, startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, inMPI, outMPI, MPIPos);
 216
 217                                 }else { //i am the child process
 218
 219                                         MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
 220                                         MPIPos.resize(numSeqs+1);
 221                                         MPI_Recv(&MPIPos[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
 222
 223                                         //figure out how many sequences you have to align
 224                                         numSeqsPerProcessor = numSeqs / processors;
 225                                         int startIndex =  pid * numSeqsPerProcessor;
 226                                         if(pid == (processors - 1)){    numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor;      }
 227
 228                                         //do your part
 229                                         MPICreateSummary(startIndex, numSeqsPerProcessor, startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, inMPI, outMPI, MPIPos);
 230                                 }
 231
 232                                 MPI_File_close(&inMPI);
 233                                 MPI_File_close(&outMPI);
 234                                 MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
 235
 236                                 if (pid == 0) {
 237                                         //get the info from the child processes
 238                                         for(int i = 1; i < processors; i++) {
 239                                                 int size;
 240                                                 MPI_Recv(&size, 1, MPI_INT, i, tag, MPI_COMM_WORLD, &status);
 241
 242                                                 vector<int> temp; temp.resize(size+1);
 243
 244                                                 for(int j = 0; j < 5; j++) {
 245
 246                                                         MPI_Recv(&temp[0], (size+1), MPI_INT, i, 2001, MPI_COMM_WORLD, &status);
 247                                                         int receiveTag = temp[temp.size()-1];  //child process added a int to the end to indicate what count this is for
 248
 249                                                         if (receiveTag == startTag) {
 250                                                                 for (int k = 0; k < size; k++) {                startPosition.push_back(temp[k]);       }
 251                                                         }else if (receiveTag == endTag) {
 252                                                                 for (int k = 0; k < size; k++) {                endPosition.push_back(temp[k]); }
 253                                                         }else if (receiveTag == lengthTag) {
 254                                                                 for (int k = 0; k < size; k++) {                seqLength.push_back(temp[k]);   }
 255                                                         }else if (receiveTag == baseTag) {
 256                                                                 for (int k = 0; k < size; k++) {                ambigBases.push_back(temp[k]);  }
 257                                                         }else if (receiveTag == lhomoTag) {
 258                                                                 for (int k = 0; k < size; k++) {                longHomoPolymer.push_back(temp[k]);     }
 259                                                         }
 260                                                 }
 261                                         }
 262
 263                                 }else{
 264
 265                                         //send my counts
 266                                         int size = startPosition.size();
 267                                         MPI_Send(&size, 1, MPI_INT, 0, tag, MPI_COMM_WORLD);
 268
 269                                         startPosition.push_back(startTag);
 270                                         int ierr = MPI_Send(&(startPosition[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
 271                                         endPosition.push_back(endTag);
 272                                         ierr = MPI_Send (&(endPosition[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
 273                                         seqLength.push_back(lengthTag);
 274                                         ierr = MPI_Send(&(seqLength[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
 275                                         ambigBases.push_back(baseTag);
 276                                         ierr = MPI_Send(&(ambigBases[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
 277                                         longHomoPolymer.push_back(lhomoTag);
 278                                         ierr = MPI_Send(&(longHomoPolymer[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
 279                                 }
 280
 281                                 MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
 282 #else
 283                         vector<unsigned long int> positions = m->divideFile(fastafile, processors);
 284
 285                         for (int i = 0; i < (positions.size()-1); i++) {
 286                                 lines.push_back(new linePair(positions[i], positions[(i+1)]));
 287                         }
 288
 289                 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 290                                 if(processors == 1){
 291                                         numSeqs = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile, lines[0]);
 292                                 }else{
 293                                         numSeqs = createProcessesCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile);
 294
 295                                         rename((summaryFile + toString(processIDS[0]) + ".temp").c_str(), summaryFile.c_str());
 296                                         //append files
 297                                         for(int i=1;i<processors;i++){
 298                                                 m->appendFiles((summaryFile + toString(processIDS[i]) + ".temp"), summaryFile);
 299                                                 remove((summaryFile + toString(processIDS[i]) + ".temp").c_str());
 300                                         }
 301                                 }
 302
 303                                 if (m->control_pressed) {  return 0; }
 304                 #else
 305                                 numSeqs = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile, lines[0]);
 306                                 if (m->control_pressed) {  return 0; }
 307                 #endif
 308 #endif
 309
 310                 #ifdef USE_MPI
 311                         if (pid == 0) {
 312                 #endif
 313
 314                 sort(startPosition.begin(), startPosition.end());
 315                 sort(endPosition.begin(), endPosition.end());
 316                 sort(seqLength.begin(), seqLength.end());
 317                 sort(ambigBases.begin(), ambigBases.end());
 318                 sort(longHomoPolymer.begin(), longHomoPolymer.end());
 319                 int size = startPosition.size();
 320
 321                 int ptile0_25   = int(size * 0.025);
 322                 int ptile25             = int(size * 0.250);
 323                 int ptile50             = int(size * 0.500);
 324                 int ptile75             = int(size * 0.750);
 325                 int ptile97_5   = int(size * 0.975);
 326                 int ptile100    = size - 1;
 327
 328                 //to compensate for blank sequences that would result in startPosition and endPostion equalling -1
 329                 if (startPosition[0] == -1) {  startPosition[0] = 0;    }
 330                 if (endPosition[0] == -1)       {  endPosition[0] = 0;          }
 331
 332                 if (m->control_pressed) {  remove(summaryFile.c_str()); return 0; }
 333
 334                 m->mothurOutEndLine();
 335                 m->mothurOut("\t\tStart\tEnd\tNBases\tAmbigs\tPolymer"); m->mothurOutEndLine();
 336                 m->mothurOut("Minimum:\t" + toString(startPosition[0]) + "\t" + toString(endPosition[0]) + "\t" + toString(seqLength[0]) + "\t" + toString(ambigBases[0]) + "\t" + toString(longHomoPolymer[0])); m->mothurOutEndLine();
 337                 m->mothurOut("2.5%-tile:\t" + toString(startPosition[ptile0_25]) + "\t" + toString(endPosition[ptile0_25]) + "\t" + toString(seqLength[ptile0_25]) + "\t" + toString(ambigBases[ptile0_25]) + "\t"+ toString(longHomoPolymer[ptile0_25])); m->mothurOutEndLine();
 338                 m->mothurOut("25%-tile:\t" + toString(startPosition[ptile25]) + "\t" + toString(endPosition[ptile25]) + "\t" + toString(seqLength[ptile25]) + "\t" + toString(ambigBases[ptile25]) + "\t" + toString(longHomoPolymer[ptile25])); m->mothurOutEndLine();
 339                 m->mothurOut("Median: \t" + toString(startPosition[ptile50]) + "\t" + toString(endPosition[ptile50]) + "\t" + toString(seqLength[ptile50]) + "\t" + toString(ambigBases[ptile50]) + "\t" + toString(longHomoPolymer[ptile50])); m->mothurOutEndLine();
 340                 m->mothurOut("75%-tile:\t" + toString(startPosition[ptile75]) + "\t" + toString(endPosition[ptile75]) + "\t" + toString(seqLength[ptile75]) + "\t" + toString(ambigBases[ptile75]) + "\t" + toString(longHomoPolymer[ptile75])); m->mothurOutEndLine();
 341                 m->mothurOut("97.5%-tile:\t" + toString(startPosition[ptile97_5]) + "\t" + toString(endPosition[ptile97_5]) + "\t" + toString(seqLength[ptile97_5]) + "\t" + toString(ambigBases[ptile97_5]) + "\t" + toString(longHomoPolymer[ptile97_5])); m->mothurOutEndLine();
 342                 m->mothurOut("Maximum:\t" + toString(startPosition[ptile100]) + "\t" + toString(endPosition[ptile100]) + "\t" + toString(seqLength[ptile100]) + "\t" + toString(ambigBases[ptile100]) + "\t" + toString(longHomoPolymer[ptile100])); m->mothurOutEndLine();
 343                 if (namefile == "") {  m->mothurOut("# of Seqs:\t" + toString(numSeqs)); m->mothurOutEndLine(); }
 344                 else { m->mothurOut("# of unique seqs:\t" + toString(numSeqs)); m->mothurOutEndLine(); m->mothurOut("total # of seqs:\t" + toString(startPosition.size())); m->mothurOutEndLine(); }
 345
 346                 if (m->control_pressed) {  remove(summaryFile.c_str()); return 0; }
 347
 348                 m->mothurOutEndLine();
 349                 m->mothurOut("Output File Name: "); m->mothurOutEndLine();
 350                 m->mothurOut(summaryFile); m->mothurOutEndLine();       outputNames.push_back(summaryFile); outputTypes["summary"].push_back(summaryFile);
 351                 m->mothurOutEndLine();
 352
 353                 #ifdef USE_MPI
 354                         }
 355                 #endif
 356
 357                 return 0;
 358         }
 359         catch(exception& e) {
 360                 m->errorOut(e, "SeqSummaryCommand", "execute");
 361                 exit(1);
 362         }
 363 }
 364 /**************************************************************************************/
 365 int SeqSummaryCommand::driverCreateSummary(vector<int>& startPosition, vector<int>& endPosition, vector<int>& seqLength, vector<int>& ambigBases, vector<int>& longHomoPolymer, string filename, string sumFile, linePair* filePos) {
 366         try {
 367
 368                 ofstream outSummary;
 369                 m->openOutputFile(sumFile, outSummary);
 370
 371                 //print header if you are process 0
 372                 if (filePos->start == 0) {
 373                         outSummary << "seqname\tstart\tend\tnbases\tambigs\tpolymer\tnumSeqs" << endl;
 374                 }
 375
 376                 ifstream in;
 377                 m->openInputFile(filename, in);
 378
 379                 in.seekg(filePos->start);
 380
 381                 bool done = false;
 382                 int count = 0;
 383
 384                 while (!done) {
 385
 386                         if (m->control_pressed) { in.close(); outSummary.close(); return 1; }
 387
 388                         Sequence current(in); m->gobble(in);
 389
 390                         if (current.getName() != "") {
 391
 392                                 int num = 1;
 393                                 if (namefile != "") {
 394                                         //make sure this sequence is in the namefile, else error
 395                                         map<string, int>::iterator it = nameMap.find(current.getName());
 396
 397                                         if (it == nameMap.end()) { m->mothurOut("[ERROR]: " + current.getName() + " is not in your namefile, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
 398                                         else { num = it->second; }
 399                                 }
 400
 401                                 //for each sequence this sequence represents
 402                                 for (int i = 0; i < num; i++) {
 403                                         startPosition.push_back(current.getStartPos());
 404                                         endPosition.push_back(current.getEndPos());
 405                                         seqLength.push_back(current.getNumBases());
 406                                         ambigBases.push_back(current.getAmbigBases());
 407                                         longHomoPolymer.push_back(current.getLongHomoPolymer());
 408                                 }
 409
 410                                 count++;
 411                                 outSummary << current.getName() << '\t';
 412                                 outSummary << current.getStartPos() << '\t' << current.getEndPos() << '\t';
 413                                 outSummary << current.getNumBases() << '\t' << current.getAmbigBases() << '\t';
 414                                 outSummary << current.getLongHomoPolymer() << '\t' << num << endl;
 415                         }
 416
 417                         #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 418                                 unsigned long int pos = in.tellg();
 419                                 if ((pos == -1) || (pos >= filePos->end)) { break; }
 420                         #else
 421                                 if (in.eof()) { break; }
 422                         #endif
 423
 424                         //report progress
 425                         //if((count) % 100 == 0){       m->mothurOut(toString(count)); m->mothurOutEndLine();           }
 426                 }
 427                 //report progress
 428                 //if((count) % 100 != 0){       m->mothurOut(toString(count)); m->mothurOutEndLine();           }
 429
 430                 in.close();
 431
 432                 return count;
 433         }
 434         catch(exception& e) {
 435                 m->errorOut(e, "SeqSummaryCommand", "driverCreateSummary");
 436                 exit(1);
 437         }
 438 }
 439 #ifdef USE_MPI
 440 /**************************************************************************************/
 441 int SeqSummaryCommand::MPICreateSummary(int start, int num, vector<int>& startPosition, vector<int>& endPosition, vector<int>& seqLength, vector<int>& ambigBases, vector<int>& longHomoPolymer, MPI_File& inMPI, MPI_File& outMPI, vector<unsigned long int>& MPIPos) {
 442         try {
 443
 444                 int pid;
 445                 MPI_Status status;
 446                 MPI_Comm_rank(MPI_COMM_WORLD, &pid);
 447
 448                 for(int i=0;i<num;i++){
 449
 450                         if (m->control_pressed) { return 0; }
 451
 452                         //read next sequence
 453                         int length = MPIPos[start+i+1] - MPIPos[start+i];
 454
 455                         char* buf4 = new char[length];
 456                         MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status);
 457
 458                         string tempBuf = buf4;
 459                         if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length);  }
 460                         istringstream iss (tempBuf,istringstream::in);
 461                         delete buf4;
 462
 463                         Sequence current(iss);
 464
 465                         if (current.getName() != "") {
 466
 467                                 int num = 1;
 468                                 if (namefile != "") {
 469                                         //make sure this sequence is in the namefile, else error
 470                                         map<string, int>::iterator it = nameMap.find(current.getName());
 471
 472                                         if (it == nameMap.end()) { cout << "[ERROR]: " << current.getName() << " is not in your namefile, please correct." << endl; m->control_pressed = true; }
 473                                         else { num = it->second; }
 474                                 }
 475
 476                                 //for each sequence this sequence represents
 477                                 for (int i = 0; i < num; i++) {
 478                                         startPosition.push_back(current.getStartPos());
 479                                         endPosition.push_back(current.getEndPos());
 480                                         seqLength.push_back(current.getNumBases());
 481                                         ambigBases.push_back(current.getAmbigBases());
 482                                         longHomoPolymer.push_back(current.getLongHomoPolymer());
 483                                 }
 484
 485                                 string outputString = current.getName() + "\t" + toString(current.getStartPos()) + "\t" + toString(current.getEndPos()) + "\t";
 486                                 outputString += toString(current.getNumBases()) + "\t" + toString(current.getAmbigBases()) + "\t" + toString(current.getLongHomoPolymer()) + "\t" + toString(num) + "\n";
 487
 488                                 //output to file
 489                                 length = outputString.length();
 490                                 char* buf3 = new char[length];
 491                                 memcpy(buf3, outputString.c_str(), length);
 492
 493                                 MPI_File_write_shared(outMPI, buf3, length, MPI_CHAR, &status);
 494                                 delete buf3;
 495                         }
 496                 }
 497
 498                 return 0;
 499         }
 500         catch(exception& e) {
 501                 m->errorOut(e, "SeqSummaryCommand", "MPICreateSummary");
 502                 exit(1);
 503         }
 504 }
 505 #endif
 506 /**************************************************************************************************/
 507 int SeqSummaryCommand::createProcessesCreateSummary(vector<int>& startPosition, vector<int>& endPosition, vector<int>& seqLength, vector<int>& ambigBases, vector<int>& longHomoPolymer, string filename, string sumFile) {
 508         try {
 509 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 510                 int process = 0;
 511                 int num = 0;
 512                 processIDS.clear();
 513
 514                 //loop through and create all the processes you want
 515                 while (process != processors) {
 516                         int pid = fork();
 517
 518                         if (pid > 0) {
 519                                 processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
 520                                 process++;
 521                         }else if (pid == 0){
 522                                 num = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, sumFile + toString(getpid()) + ".temp", lines[process]);
 523
 524                                 //pass numSeqs to parent
 525                                 ofstream out;
 526                                 string tempFile = fastafile + toString(getpid()) + ".num.temp";
 527                                 m->openOutputFile(tempFile, out);
 528
 529                                 out << num << endl;
 530                                 out << startPosition.size() << endl;
 531                                 for (int k = 0; k < startPosition.size(); k++)          {               out << startPosition[k] << '\t'; }  out << endl;
 532                                 for (int k = 0; k < endPosition.size(); k++)            {               out << endPosition[k] << '\t'; }  out << endl;
 533                                 for (int k = 0; k < seqLength.size(); k++)                      {               out << seqLength[k] << '\t'; }  out << endl;
 534                                 for (int k = 0; k < ambigBases.size(); k++)                     {               out << ambigBases[k] << '\t'; }  out << endl;
 535                                 for (int k = 0; k < longHomoPolymer.size(); k++)        {               out << longHomoPolymer[k] << '\t'; }  out << endl;
 536
 537                                 out.close();
 538
 539                                 exit(0);
 540                         }else {
 541                                 m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
 542                                 for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
 543                                 exit(0);
 544                         }
 545                 }
 546
 547                 //force parent to wait until all the processes are done
 548                 for (int i=0;i<processors;i++) {
 549                         int temp = processIDS[i];
 550                         wait(&temp);
 551                 }
 552
 553                 //parent reads in and combine Filter info
 554                 for (int i = 0; i < processIDS.size(); i++) {
 555                         string tempFilename = fastafile + toString(processIDS[i]) + ".num.temp";
 556                         ifstream in;
 557                         m->openInputFile(tempFilename, in);
 558
 559                         int temp, tempNum;
 560                         in >> tempNum; m->gobble(in); num += tempNum;
 561                         in >> tempNum; m->gobble(in);
 562                         for (int k = 0; k < tempNum; k++)                       {               in >> temp; startPosition.push_back(temp);              }               m->gobble(in);
 563                         for (int k = 0; k < tempNum; k++)                       {               in >> temp; endPosition.push_back(temp);                }               m->gobble(in);
 564                         for (int k = 0; k < tempNum; k++)                       {               in >> temp; seqLength.push_back(temp);                  }               m->gobble(in);
 565                         for (int k = 0; k < tempNum; k++)                       {               in >> temp; ambigBases.push_back(temp);                 }               m->gobble(in);
 566                         for (int k = 0; k < tempNum; k++)                       {               in >> temp; longHomoPolymer.push_back(temp);    }               m->gobble(in);
 567
 568                         in.close();
 569                         remove(tempFilename.c_str());
 570                 }
 571
 572                 return num;
 573 #endif
 574         }
 575         catch(exception& e) {
 576                 m->errorOut(e, "SeqSummaryCommand", "createProcessesCreateSummary");
 577                 exit(1);
 578         }
 579 }
 580 /**********************************************************************************************************************/
 581
 582
 583