// Marth Lab, Department of Biology, Boston College
// All rights reserved.
// ---------------------------------------------------------------------------
-// Last modified: 21 March 2011 (DB)
+// Last modified: 18 April 2011 (DB)
// ---------------------------------------------------------------------------
// Provides functionality for validating SamHeader data
// ***************************************************************************
using namespace BamTools;
using namespace BamTools::Internal;
+#include <cctype>
#include <iostream>
#include <set>
#include <sstream>
using namespace std;
+namespace BamTools {
+namespace Internal {
+
+bool caseInsensitiveCompare(const string& lhs, const string& rhs) {
+
+ // can omit checking chars if lengths not equal
+ const int lhsLength = lhs.length();
+ const int rhsLength = rhs.length();
+ if ( lhsLength != rhsLength )
+ return false;
+
+ // do *basic* toupper checks on each string char's
+ for ( int i = 0; i < lhsLength; ++i ) {
+ if ( toupper( (int)lhs.at(i)) != toupper( (int)rhs.at(i)) )
+ return false;
+ }
+
+ // otherwise OK
+ return true;
+}
+
+} // namespace Internal
+} // namespace BamTools
+
// ------------------------------------------------------------------------
// Allow validation rules to vary, as needed, between SAM header versions
//
// // use rule introduced with version 2.0
static const SamHeaderVersion SAM_VERSION_1_0 = SamHeaderVersion(1,0);
+static const SamHeaderVersion SAM_VERSION_1_1 = SamHeaderVersion(1,1);
+static const SamHeaderVersion SAM_VERSION_1_2 = SamHeaderVersion(1,2);
static const SamHeaderVersion SAM_VERSION_1_3 = SamHeaderVersion(1,3);
+static const SamHeaderVersion SAM_VERSION_1_4 = SamHeaderVersion(1,4);
// TODO: This functionality is currently unused.
// Make validation "version-aware."
isValid &= ValidateMetadata();
isValid &= ValidateSequenceDictionary();
isValid &= ValidateReadGroupDictionary();
- isValid &= ValidateProgramData();
+ isValid &= ValidateProgramChain();
// report errors if desired
if ( verbose ) {
bool SamHeaderValidator::ValidateSequenceDictionary(void) {
- // TODO: warn/error if no sequences ?
-
bool isValid = true;
// check for unique sequence names
SamSequenceConstIterator seqEnd = sequences.ConstEnd();
for ( ; seqIter != seqEnd; ++seqIter ) {
const SamSequence& seq = (*seqIter);
- const string& name = seq.Name;
// lookup sequence name
+ const string& name = seq.Name;
nameIter = sequenceNames.find(name);
// error if found (duplicate entry)
bool SamHeaderValidator::ValidateReadGroupDictionary(void) {
- // TODO: warn/error if no read groups ?
-
bool isValid = true;
// check for unique read group IDs & platform units
return true;
// if technology is valid keyword
- if ( Is454(technology) ||
- IsHelicos(technology) ||
- IsIllumina(technology) ||
- IsPacBio(technology) ||
- IsSolid(technology)
+ if ( caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_CAPILLARY) ||
+ caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_HELICOS) ||
+ caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_ILLUMINA) ||
+ caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_IONTORRENT) ||
+ caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_LS454) ||
+ caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_PACBIO) ||
+ caseInsensitiveCompare(technology, Constants::SAM_RG_SEQTECHNOLOGY_SOLID)
)
{
return true;
return false;
}
-bool SamHeaderValidator::Is454(const string& technology) {
- return ( technology == Constants::SAM_RG_SEQTECHNOLOGY_454 ||
- technology == Constants::SAM_RG_SEQTECHNOLOGY_LS454_LOWER ||
- technology == Constants::SAM_RG_SEQTECHNOLOGY_LS454_UPPER
- );
-}
-
-bool SamHeaderValidator::IsHelicos(const string& technology) {
- return ( technology == Constants::SAM_RG_SEQTECHNOLOGY_HELICOS_LOWER ||
- technology == Constants::SAM_RG_SEQTECHNOLOGY_HELICOS_UPPER
- );
-}
-
-bool SamHeaderValidator::IsIllumina(const string& technology) {
- return ( technology == Constants::SAM_RG_SEQTECHNOLOGY_ILLUMINA_LOWER ||
- technology == Constants::SAM_RG_SEQTECHNOLOGY_ILLUMINA_UPPER
- );
-}
-
-bool SamHeaderValidator::IsPacBio(const string& technology) {
- return ( technology == Constants::SAM_RG_SEQTECHNOLOGY_PACBIO_LOWER ||
- technology == Constants::SAM_RG_SEQTECHNOLOGY_PACBIO_UPPER
- );
-}
-
-bool SamHeaderValidator::IsSolid(const string& technology) {
- return ( technology == Constants::SAM_RG_SEQTECHNOLOGY_SOLID_LOWER ||
- technology == Constants::SAM_RG_SEQTECHNOLOGY_SOLID_UPPER
- );
-}
-
-bool SamHeaderValidator::ValidateProgramData(void) {
+bool SamHeaderValidator::ValidateProgramChain(void) {
bool isValid = true;
isValid &= ContainsUniqueProgramIds();
isValid &= ValidatePreviousProgramIds();
}
bool SamHeaderValidator::ContainsUniqueProgramIds(void) {
+
bool isValid = true;
- // TODO: once we have ability to handle multiple @PG entries,
- // check here for duplicate ID's
- // but for now, just return true
+ set<string> programIds;
+ set<string>::iterator pgIdIter;
+
+ // iterate over program records
+ const SamProgramChain& programs = m_header.Programs;
+ SamProgramConstIterator pgIter = programs.ConstBegin();
+ SamProgramConstIterator pgEnd = programs.ConstEnd();
+ for ( ; pgIter != pgEnd; ++pgIter ) {
+ const SamProgram& pg = (*pgIter);
+
+ // lookup program ID
+ const string& pgId = pg.ID;
+ pgIdIter = programIds.find(pgId);
+
+ // error if found (duplicate entry)
+ if ( pgIdIter != programIds.end() ) {
+ AddError("Program ID (ID): " + pgId + " is not unique");
+ isValid = false;
+ }
+
+ // otherwise ok, store ID
+ programIds.insert(pgId);
+ }
+
+ // return validation state
return isValid;
}
bool SamHeaderValidator::ValidatePreviousProgramIds(void) {
+
bool isValid = true;
- // TODO: check that PP entries are valid later, after we get multiple @PG-entry handling
- // just return true for now
+
+ // iterate over program records
+ const SamProgramChain& programs = m_header.Programs;
+ SamProgramConstIterator pgIter = programs.ConstBegin();
+ SamProgramConstIterator pgEnd = programs.ConstEnd();
+ for ( ; pgIter != pgEnd; ++pgIter ) {
+ const SamProgram& pg = (*pgIter);
+
+ // ignore record for validation if PreviousProgramID is empty
+ const string& ppId = pg.PreviousProgramID;
+ if ( ppId.empty() )
+ continue;
+
+ // see if program "chain" contains an entry for ppId
+ if ( !programs.Contains(ppId) ) {
+ AddError("PreviousProgramID (PP): " + ppId + " is not a known ID");
+ isValid = false;
+ }
+ }
+
+ // return validation state
return isValid;
}
void SamHeaderValidator::AddError(const string& message) {
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