[ape.git] / ChangeLog

                CHANGES IN APE VERSION 2.5-1


NEW FEATURES

    o The new function stree generates trees with regular shapes.

    o It is now possible to bind two trees with x + y (see ?bind.tree for
      details).

    o drop.tip(), extract.clade(), root(), and bind.tree() now have an
      'interactive' option to make the operation on a plotted tree.


BUG FIXES

    o rTraitDisc() did not use its 'freq' argument correctly (it was
      multiplied with the rate matrix column-wise instead of row-wise).

    o [node|tip|edge]labels(thermo = ) used to draw empty thermometers
      with NA values. Nothing is drawn now like with 'text' or 'pch'.
      The same bug occurred with the 'pie' option.

    o A bug was fixed in compar.ou() and the help page was clarified.

    o bind.tree() has been rewritten fixing several bugs and making it
      more efficient.

    o plot.phylo(type = "p") sometimes failed to colour correctly the
      vertical lines representing the nodes.



        CHANGES IN APE VERSION 2.5


NEW FEATURES

    o The new function parafit by Pierre Legendre tests for the
      coevolution between hosts and parasites. It has a companion
      function, pcoa, that does principal coordinate decomposition.
      The latter has a biplot method.

    o The new function lmorigin by Pierre Legendre performs multiple
      regression through the origin with testing by permutation.

    o The new functions rTraitCont and rTraitDisc simulate continuous and
      discrete traits under a wide range of evolutionary models.

    o The new function delta.plot does a delta plot following Holland et
      al. (2002, Mol. Biol. Evol. 12:2051).

    o The new function edges draws additional branches between any nodes
      and/or tips on a plotted tree.

    o The new function fancyarrows enhances arrows from graphics with
      triangle and harpoon heads; it can be called from edges().

    o add.scale.bar() has a new option 'ask' to draw interactively.

    o The branch length score replaces the geodesic distance in dist.topo.

    o Three new data sets are included: the gopher-lice data (gopher.D),
      SO2 air pollution in 41 US cities (lmorigin.ex1, from Sokal &
      Rohlf 1995), and some host-parasite specificity data
      (lmorigin.ex2, from Legendre & Desdevises 2009).


BUG FIXES

    o add.scale.bar() drew the bar outside the plotting region with the
      default options with unrooted or radial trees.

    o dist.topo() made R stuck when the trees had different sizes (thanks
      to Otto Cordero for the fix).


OTHER CHANGES

    o The geodesic distance has been replaced by the branch length score
      in dist.topo().



                CHANGES IN APE VERSION 2.4-1


NEW FEATURES

    o rtree() and rcoal() now accept a numeric vector for the 'br'
      argument.

    o vcv() is a new generic function with methods for the classes "phylo"
      and "corPhyl" so that it is possible to calculate the var-cov matrix
      for "transformation models". vcv.phylo() can still be used for trees
      of class "phylo"; its argument 'cor' has been renamed 'corr'.


BUG FIXES

    o bind.tree() failed when 'y' had no root edge.

    o read.nexus() shuffled tip labels when the trees have no branch
      lengths and there is a TRANSLATE block.

    o read.nexus() does not try to translate node labels if there is a
      translation table in the NEXUS file. See ?read.nexus for a
      clarification on this behaviour.

    o plot.multiPhylo() crashed R when plotting a list of trees with
      compressed tip labels.

    o write.nexus() did not translate the taxa names when asked for.

    o plot.phylo(type = "fan") did not rotate the tip labels correctly
      when the tree has branch lengths.

    o ace(type = "continuous", method = "ML") now avoids sigma² being
      negative (which resulted in an error).

    o nj() crashed with NA/NaN in the distance matrix: an error in now
      returned.



                CHANGES IN APE VERSION 2.4


NEW FEATURES

    o base.freq() has a new option 'freq' to return the counts; the
      default is still to return the proportions.


BUG FIXES

    o seg.sites() did not handle ambiguous nucleotides correctly: they
      are now ignored.

    o plot(phy, root.edge = TRUE) failed if there was no $root.edge in
      the tree: the argument is now ignored.

    o add.scale.bar() failed when 'x' and 'y' were given (thanks to Janet
      Young for the fix).


OTHER CHANGES

    o Trying to plot a tree with a single tip now returns NULL with a
      warning (it returned an error previously).

    o The way lines representing nodes are coloured in phylograms has
      been modified (as well as their widths and types) following some
      users' request; this is only for dichotomous nodes.

    o The argument 'adj' in [node][tip][edge]labels() now works when
      using 'pie' or 'thermo'.

    o A more informative message error is now returned by dist.dna() when
      'model' is badly specified (partial matching of this argument is
      done now).

    o Deprecated functions are now listed in a help page: see
      help("ape-defunct") with the quotes.


DEPRECATED & DEFUNCT

    o The functions heterozygosity, nuc.div, theta.h, theta.k and
      theta.s have been moved from ape to pegas.

    o The functions mlphylo, DNAmodel and sh.test have been removed.



                CHANGES IN APE VERSION 2.3-3


BUG FIXES

    o add.scale.bar() always drew a horizontal bar.

    o zoom() shuffled tips with unrooted trees.

    o write.nexus() failed to write correctly trees with a "TipLabel"
      attribute.

    o rcoal() failed to compute branch lengths with very large n.

    o A small bug was fixed in compar.cheverud() (thanks to Michael
      Phelan for the fix).

    o seg.sites() failed when passing a vector.

    o drop.tip() sometimes shuffled tip labels.

    o root() shuffled node labels with 'resolve.root = TRUE'.



                CHANGES IN APE VERSION 2.3-2


BUG FIXES

    o all.equal.phylo() did not compare unrooted trees correctly.

    o dist.topo(... method = "PH85") did not treat unrooted trees
      correctly (thanks to Tim Wallstrom for the fix).

    o root() sometimes failed to test for the monophyly of the
      outgroup correctly.

    o extract.clade() sometimes included too many edges.

    o vcv.phylo() did not work correctly when the tree is in
      "pruningwise" order.

    o nj() did not handle correctly distance matrices with many 0's.
      The code has also been significantly improved: 7, 70, 160 times
      faster with n = 100, 500, 1000, respectively.



                CHANGES IN APE VERSION 2.3-1


NEW FEATURES

    o The new function is.monophyletic tests the monophyly of a group.

    o There is now a c() method for lists of class "DNAbin".

    o yule.cov() now fits the null model, and its help page has been
      corrected with respect to this change.

    o drop.tip() has a new option 'rooted' to force (or not) a tree
      to be treated as (un)rooted.


BUG FIXES

    o dist.gene() failed on most occasions with the default
      pairwise.deletion = FALSE.

    o read.tree() failed to read correctly the tree name(s).

    o boot.phylo() now treats correctly data frames.

    o del.gaps() did not copy the rownames of a matrix.

    o A small bug was fixed in CDAM.global().

    o ace() failed with large data sets. Thanks to Rich FitzJohn for
      the fix. With other improvements, this function is now about 6
      times faster.

    o write.tree() failed with objects of class "multiPhylo".

    o drop.tip(, subtree = TRUE) sometimes shuffled tip labels.


OTHER CHANGES

    o [.multiPhylo and [.DNAbin now respect the original class.

    o Instances of the form class(phy) == "phylo" have been replaced
      by inherits(phy, "phylo").

    o rcoal() is now faster.


DEPRECATED & DEFUNCT

    o klastorin() has been removed.



                CHANGES IN APE VERSION 2.3


NEW FEATURES

    o The new functions CADM.global and CADM.post, contributed by
      Pierre Legendre, test the congruence among several distance
      matrices.

    o The new function yule.time fits a user-defined time-dependent
      Yule model by maximum likelihood.

    o The new function makeNodeLabel creates and/or modifies node
      labels in a flexible way.

    o read.tree() and write.tree() have been modified so that they can
      handle individual tree names.

    o plot.phylo() has a new argument 'edge.lty' that specifies the
      types of lines used for the edges (plain, dotted, dashed, ...)

    o phymltest() has been updated to work with PhyML 3.0.1.


BUG FIXES

    o drop.tip() shuffled tip labels in some cases.

    o drop.tip() did not handle node.label correctly.

    o is.ultrametric() now checks the ordering of the edge matrix.

    o ace() sometimes returned negative values of likelihoods of
      ancestral states (thanks to Dan Rabosky for solving this long
      lasting bug).


OTHER CHANGES

    o The data set xenarthra has been removed.



                CHANGES IN APE VERSION 2.2-4

BUG FIXES

    o The bug fix in read.nexus() in version 2.2-3 was wrong: this is
      now fixed. (Thanks to Peter Wragg for the fix!)

    o A warning message occurred for no reason with ace(method="GLS").


OTHER CHANGES

    o There is now a general help page displayed with '?ape'.



                CHANGES IN APE VERSION 2.2-3


NEW FEATURES

    o The new function extract.clade extracts a clade from a tree by
      specifying a node number or label.

    o fastme.bal() has two new options 'spr' and 'tbr' to perform tree
      operations of the same names.

    o dist.dna() can now return the number of site differences by
      specifying model="N".


BUG FIXES

    o chronopl() did not work with CV = TRUE.

    o read.nexus() did not work correctly in some situations (trees on
      multiple lines with different numbers of lines and/or with
      comments inserted within the trees).

    o ltt.plot(), ltt.lines(), and mltt.plot() did not count correctly
      the number of lineages with non-binary trees.


OTHER CHANGES

    o ape has now a namespace.

    o drop.tip() has been improved: it should be much faster and work
      better in some cases (e.g., see the example in ?zoom).



                CHANGES IN APE VERSION 2.2-2


NEW FEATURES

    o dist.gene() has been substantially improved and gains an option
      'pairwise.deletion'.

    o cbind.DNAbin() has a new option 'fill.with.gaps' and is now
      more flexible.


BUG FIXES

    o prop.part() failed with a single tree with the default option
     'check.labels = TRUE'.

   o summary.DNAbin() failed to display correctly the summary of
     sequence lengths with lists of sequences of 10,000 bases or more
     (because summary.default uses 4 significant digits by default).

   o read.nexus() failed to read a file with a single tree with line
     breaks in the Newick string.

   o del.gaps() returned a list of empty sequences when there were no
     gaps.


OTHER CHANGES

    o phymltest() has been updated for PhyML 3.0 and gains an option
      'append', whereas the option 'path2exec' has been removed.

    o rbind.DNAbin() and cbind.DNAbin() now accept a single matrix
      which is returned unchanged (instead of an error).

    o The data sets bird.orders and bird.families are now stored as
      Newick strings; i.e., the command data(bird.orders) calls
      read.tree().



                CHANGES IN APE VERSION 2.2-1


NEW FEATURES

    o The new function makeLabel() helps to modify labels of trees,
      lists of trees, or DNA sequences, with several utilities to
      truncate and/or make them unique, substituting some
      characters, and so on.

    o The new function del.gaps() removes insertion gaps ("-") in a
      set of DNA sequences.

    o read.dna() can now read Clustal files (*.aln).


BUG FIXES

    o root() failed with 'resolve.root = TRUE' when the root was
      already the specified root.

    o Several bugs were fixed in mlphylo().

    o collapsed.singles() did not propagate the 'Nnode' and
      'node.labels' elements (thanks to Elizabeth Purdom for the fix).

    o read.nexus() failed to remove correctly the comments within
      trees.

    o read.nexus() failed to read a file with a single tree and no
      translation of tip labels.

    o read.nexus() failed to place correctly tip labels when reading
      a single tree with no edge lengths.

    o A bug was fixed in sh.test().


OTHER CHANGES

    o unique.multiPhylo() is faster thanks to a suggestion by Vladimir
      Minin.

    o The option 'check.labels' of consensus() and prop.part() is now
      TRUE by default.

    o write.dna() now does not truncate names to 10 characters with
      the Phylip formats.



                CHANGES IN APE VERSION 2.2


NEW FEATURES

    o Four new functions have been written by Damien de Vienne for the
      graphical exploration of large trees (cophyloplot, subtrees,
      subtreeplot), and to return the graphical coordinates of tree
      (without plotting).

    o The new functions corPagel and corBlomberg implement the Pagel's
      "lambda" and Blomberg et al.'s "ACDC" correlation structures.

    o chronopl() has been improved and gains several options: see its
      help page for details.

    o boot.phylo() has now an option 'trees' to possibly return the
      bootstraped trees (the default is FALSE).

    o prop.part() has been improved and should now be faster in all
      situations.


BUG FIXES

    o read.dna() failed if "?" occurred in the first 10 sites of the
      first sequence.

    o The x/y aspect of the plot is now respected when plotting a
      circular tree (type = "r" or "f").

    o Drawing the tip labels sometimes failed when plotting circular
      trees.

    o zoom() failed when tip labels were used instead of their numbers
      (thanks to Yan Wong for the fix).

    o drop.tip() failed with some trees (fixed by Yan Wong).

    o seg.sites() failed with a list.

    o consensus() failed in some cases. The function has been improved
      as well and is faster.



                CHANGES IN APE VERSION 2.1-3


BUG FIXES

    o A bug in read.nexus() made the Windows R-GUI crash.

    o An error was fixed in the computation of ancestral character
      states by generalized least squares in ace().

    o di2multi() did not modify node labels correctly.

    o multi2di() failed if the tree had its attribute "order" set to
      "cladewise".



                CHANGES IN APE VERSION 2.1-2


NEW FEATURES

    o There three new methods for the "multiPhylo" class: str, $,
      and [[.

    o root() gains the options 'node' and 'resolve.root'
      (FALSE by default) as well as its code being improved.

    o mltt.plot() has now an option 'log' used in the same way
      than in plot.default().


BUG FIXES

    o mltt.plot() failed to display the legend with an unnamed
      list of trees.

    o nodelabels() with pies now correcly uses the argument
      'cex' to draw symbols of different sizes (which has
      worked already for thermometers).

    o read.nexus() generally failed to read very big files.


OTHER CHANGES

    o The argument 'family' of compar.gee() can now be a function
      as well as a character string.

    o read.tree() and read.nexus() now return an unnamed list if
      'tree.names = NULL'.

    o read.nexus() now returns a modified object of class "multiPhylo"
      when there is a TRANSLATE block in the NEXUS file: the individual
      trees have no 'tip.label' vector, but the list has a 'TipLabel'
      attribute. The new methods '$' and '[[' set these elements
      correctly when extracting trees.



                CHANGES IN APE VERSION 2.1-1


NEW FEATURES

    o The new function rmtree generates lists of random trees.

    o rcoal() now generates a genuine coalescent tree by default
      (thanks to Vladimir Minin for the code).


BUG FIXES

    o nuc.div() returned an incorrect value with the default
      pairwise.deletion = FALSE.


OTHER CHANGES

    o The internal codes of bionj(), fastme.bal(), and fastme.ols()
      have been improved so that they are stabler and faster.

    o R packages used by ape are now loaded silently; lattice and gee
      are loaded only when needed.



                CHANGES IN APE VERSION 2.1


NEW FEATURES

    o The new function identify.phylo identifies clades on a plotted
      tree using the mouse.

    o It is now possible to subset a list of trees (object of class
      "multiPhylo") with "[" while keeping its class correct.

    o The new function as.DNAbin.alignment converts DNA sequences
      stored in the "alignment" format of the package seqinr into
      an object of class "DNAbin".

    o The new function weight.taxo2 helps to build similarity matrices
      given two taxonomic levels (usually called by other functions).

    o write.tree() can now take a list of trees (class "multiPhylo")
      as its main argument.

    o plot.correlogram() and plot.correlogramList() have been
      improved, and gain several options (see the help page for
      details). A legend is now plotted by default.


BUG FIXES

    o dist.dna() returned some incorrect values with `model = "JC69"'
      and `pairwise.deletion = TRUE'. This affected only the
      distances involving sequences with missing values. (Thanks
      to Bruno Toupance for digging this bug out.)

    o write.tree() failed with some trees: this is fixed by removing
      the `multi.line' option (trees are now always printed on a
      single line).

    o read.nexus() did not correctly detect trees with multiple root
      edges (see OTHER CHANGES).


OTHER CHANGES

    o The code of mlphylo() has been almost entirely rewritten, and
      should be much stabler. The options have been also greatly
      simplified (see ?mlphylo and ?DNAmodel for details).

    o The internal function nTips has been renamed klastorin_nTips.

    o The code of is.ultrametric() contained redundancies and has
      been cleaned-up.

    o The code of Moran.I() and of correlogram.formula() have been
      improved.

    o read.tree() and read.nexus() now return an error when trying to
      read a tree with multiple root edges (see BUG FIXES). The
      correction applied in previous version did not work in all
      situations.

    o The class c("multi.tree", "phylo") has been renamed
      "multiPhylo".


DOCUMENTATION

    o There is now a vignette in ape: see vignette("MoranI", "ape").


DEPRECATED & DEFUNCT

    o as.matching() and as.phylo.matching() do not support branch
      lengths.

    o correlogram.phylo() and discrete.dist() have been removed.



                CHANGES IN APE VERSION 2.0-2


NEW FEATURES

    o The new function matexpo computes the exponential of a square
      matrix.

    o The new function unique.multi.tree removes duplicate trees from
      a list.

    o yule() has a new option `use.root.edge = FALSE' that specifies
      to ignore, by default, the root edge of the tree if it exists.


BUG FIXES

    o which.edge() failed when the index of a single terminal edge was
      looked for.

    o In diversi.time(), the values returned for model C were
      incorrect.

    o A bug was fixed in yule() that affected the calculation of the
      likelihood in the presence of ties in the branching times.

    o There was a bug in the C function mat_expo4x4 affecting the
      calculations of the transition probabilities for models HKY and
      GTR in mlphylo().

    o A small bug was fixed in as.matrix.DNAbin (thanks to James
      Bullard).

    o rtree() did not `shuffle' the tip labels by default, so only a
      limited number of labelled topologies could be generated.



                CHANGES IN APE VERSION 2.0-1


NEW FEATURES

    o The three new functions bionj, fastme.ols, and fastme.bal
      perform phylogeny estimation by the BIONJ and fastME methods in
      OLS and balanced versions. This is a port to R of previous
      previous programs done by Vincent Lefort.

    o The new function chronoMPL performs molecular dating with the
      mean path lengths method of Britton et al. (2002, Mol. Phyl.
      Evol. 24: 58).

    o The new function rotate, contributed by Christoph Heibl, swaps
      two clades connected to the same node. It works also with
      multichotomous nodes.

    o The new `method' as.matrix.DNAbin() may be used to convert
      easily DNA sequences stored in a list into a matrix while
      keeping the names and the class.


BUG FIXES

    o chronopl() failed when some branch lengths were equal to zero:
      an error message is now returned.

    o di2multi() failed when there was a series of consecutive edges
      to remove.



                CHANGES IN APE VERSION 1.10-2


NEW FEATURES

    o plot.phylo() can now plot circular trees: the option is type =
      "fan" or type = "f" (to avoid the ambiguity with type = "c").

    o prop.part() has a new option `check.labels = FALSE' which allows
      to considerably speed-up the calculations of bipartitions. As a
      consequence, calculations of bootstrap values with boot.phylo()
      should be much faster.


BUG FIXES

    o read.GenBank() did not return correctly the list of species as
      from ape 1.10: this is fixed in this version

    o Applying as.phylo() on a tree of class "phylo" failed: the
      object is now returned unchanged.



                CHANGES IN APE VERSION 1.10-1


NEW FEATURES

    o The three new functions Ntip, Nnode, and Nedge return, for a
      given tree, the number of tips, nodes, or edges, respectively.


BUG FIXES

    o read.nexus() did not set correctly the class of the returned
      object when reading multiple trees.

    o mllt.plot() failed with objects of class c("multi.tree",
      "phylo").

    o unroot() did not work correctly in most cases.

    o reorder.phylo() made R freeze in some occasions.

    o Plotting a tree in pruningwise order failed.

    o When plotting an unrooted tree, the tip labels where not all
      correctly positioned if the option `cex' was used.



                CHANGES IN APE VERSION 1.10


NEW FEATURES

    o Five new `method' functions have been introduced to manipulate
      DNA sequences in binary format (see below).

    o Three new functions have been introduced to convert between the
      new binary and the character formats.

    o The new function as.alignment converts DNA sequences stored as
      single characters into the class "alignment" used by the package
      seqinr.

    o read.dna() and read.GenBank() have a new argument `as.character'
      controlling whether the sequences are returned in binary format
      or as character.


BUG FIXES

    o root() failed when the tree had node labels: this is fixed.

    o plot.phylo() did not correctly set the limits on the y-axis with
      the default setting: this is fixed.

    o dist.dna() returned a wrong result for the LogDet, paralinear,
      and BH87 models with `pairwise.deletion = TRUE'.


OTHER CHANGES

    o DNA sequences are now internally stored in a binary format. See
      the document "A Bit-Level Coding Scheme for Nucleotides" for the
      details. Most functions analyzing DNA functions have been
      modified accordingly and are now much faster (dist.dna is now
      ca. 60 times faster).



                CHANGES IN APE VERSION 1.9-4


BUG FIXES

    o A bug was fixed in edgelabels().

    o as.phylo.hclust() did not work correctly when the object of
      class "hclust" has its labels set to NULL: the returned tree has
      now its tip labels set to "1", "2", ...

    o consensus could fail if some tip labels are a subset of others
      (e.g., "a" and "a_1"): this is now fixed.

    o mlphylo() failed in most cases if some branch lengths of the
      initial tree were greater than one: an error message is now
      issued.

    o mlphylo() failed in most cases when estimating the proportion of
      invariants: this is fixed.



                CHANGES IN APE VERSION 1.9-3


NEW FEATURES

    o The new function edgelabels adds labels on the edge of the tree
      in the same way than nodelabels or tiplabels.


BUG FIXES

    o multi2di() did not handle correctly branch lengths with the
      default option `random = TRUE': this is now fixed.

    o A bug was fixed in nuc.div() when using pairwise deletions.

    o A bug occurred in the analysis of bipartitions with large
      numbers of large trees, with consequences on prop.part,
      prop.clades, and boot.phylo.

    o The calculation of the Billera-Holmes-Vogtmann distance in
      dist.topo was wrong: this has been fixed.



                CHANGES IN APE VERSION 1.9-2


NEW FEATURES

    o The new function ladderize reorganizes the internal structure of
      a tree to plot them left- or right-ladderized.

    o The new function dist.nodes computes the patristic distances
      between all nodes, internal and terminal, of a tree. It replaces
      the option `full = TRUE' of cophenetic.phylo (see below).


BUG FIXES

    o A bug was fixed in old2new.phylo().

    o Some bugs were fixed in chronopl().

    o The edge colours were not correctly displayed by plot.phylo
      (thank you to Li-San Wang for the fix).

    o cophenetic.phylo() failed with multichotomous trees: this is
      fixed.


OTHER CHANGES

    o read.dna() now returns the sequences in a matrix if they are
      aligned (interleaved or sequential format). Sequences in FASTA
      format are still returned in a list.

    o The option `full' of cophenetic.phylo() has been removed because
      it could not be used from the generic.


DEPRECATED & DEFUNCT

    o rotate() has been removed; this function did not work correctly
      since ape 1.9.



                CHANGES IN APE VERSION 1.9-1


BUG FIXES

    o Trees with a single tip were not read correctly in R as the
      element `Nnode' was not set: this is fixed.

    o unroot() did not set correctly the number of nodes of the
      unrooted tree in most cases.

    o read.GenBank() failed when fetching very long sequences,
      particularly of the BX-series.

    o A bug was introduced in read.tree() with ape 1.9: it has been
      fixed



                CHANGES IN APE VERSION 1.9


NEW FEATURES

    o There are two new print `methods' for trees of class "phylo" and
      lists of trees of class "multi.tree", so that they are now
      displayed in a compact and informative way.

    o There are two new functions, old2new.phylo and new2old.phylo,
      for converting between the old and new coding of the class
      "phylo".

    o dist.dna() has three new models: Barry and Hartigan ("BH87"),
      LogDet ("logdet"), and paralinear ("paralin").

    o compute.brlen() has been extended: several methods are now
      available to compute branch lengths.

    o write.dna() can now handle matrices as well as lists.


BUG FIXES

    o cophenetic.phylo() sometimes returned a wrong result with
      multichotomous trees: this is fixed.

    o rotate() failed when a single tip was specified: the tree is now
      returned unchanged.

    o ace() did not return the correct index matrix with custom
      models: this is fixed.

    o multi2di() did not work correctly when resolving multichotomies
      randomly: the topology was always the same, only the arrangement
      of clades was randomized: this is fixed. This function now
      accepts trees with no branch lengths.

    o The output of diversi.gof() was blurred by useless prints when a
      user distribution was specified. This has been corrected, and
      the help page of this function has been expanded.


OTHER CHANGES

    o The internal structure of the class "phylo" has been changed:
      see the document "Definition of Formats for Coding Phylogenetic
      Trees in R" for the details. In addition, the code of most
      functions has been improved.

    o Several functions have been improved by replacing some R codes
      by C codes: pic, plot.phylo, and reorder.phylo.

    o There is now a citation information: see citation("ape") in R.

    o write.tree() now does not add extra 0's to branch lengths so
      that 1.23 is printed "1.23" by default, not "1.2300000000".

    o The syntax of bind.tree() has been simplified. This function now
      accepts trees with no branch lengths, and handles correctly node
      labels.

    o The option `as.numeric' of mrca() has been removed.

    o The unused options `format' and `rooted' of read.tree() have
      been removed.

    o The unused option `format' of write.tree() has been removed.

    o The use of node.depth() has been simplified.



                CHANGES IN APE VERSION 1.8-5


NEW FEATURES

    o Two new functions read.nexus.data() and write.nexus.data(),
      contributed by Johan Nylander, allow to read and write molecular
      sequences in NEXUS files.

    o The new function reorder.phylo() reorders the internal structure
      of a tree of class "phylo". It is used as the generic, e.g.,
      reorder(tr).

    o read.tree() and read.nexus() can now read trees with a single
      edge.

    o The new data set `cynipids' supplies a set of protein sequences
      in NEXUS format.


BUG FIXES

    o The code of all.equal.phylo() has been completely rewritten
      (thanks to Benoît Durand) which fixes several bugs.

    o read.tree() and read.nexus() now checks the labels of the tree
      to remove or substitute any characters that are illegal in the
      Newick format (parentheses, etc.)

    o A negative P-value could be returned by mantel.test(): this is
      now fixed.



                CHANGES IN APE VERSION 1.8-4


NEW FEATURES

    o The new function sh.test() computes the Shimodaira-
      Hasegawa test.

    o The new function collapse.singles() removes the nodes with a
      single descendant from a tree.

    o plot.phylo() has a new argument `tip.color' to specify the
      colours of the tips.

    o mlphylo() has now an option `quiet' to control the display of
      the progress of the analysis (the default is FALSE).


BUG FIXES

    o read.dna() did not read correctly sequences in sequential format
      with leading alignment gaps "-": this is fixed.

    o ace() returned a list with no class so that the generic
      functions (anova, logLik, ...) could not be used directly. This
      is fixed as ace() now returns an object of class "ace".

    o anova.ace() had a small bug when computing the number of degrees
      of freedom: this is fixed.

    o mlphylo() did not work when the sequences were in a matrix or
      a data frame: this is fixed.

    o rtree() did not work correctly when trying to simulate an
      unrooted tree with two tips: an error message is now issued.


OTHER CHANGES

    o The algorithm of rtree() has been changed: it is now about 40,
      100, and 130 times faster for 10, 100, and 1000 tips,
      respectively.



                CHANGES IN APE VERSION 1.8-3


NEW FEATURES

    o There are four new `method' functions to be used with the
      results of ace(): logLik(), deviance(), AIC(), and anova().

    o The plot method of phymltest has two new arguments: `main' to
      change the title, and `col' to control the colour of the
      segments showing the AIC values.

    o ace() has a new argument `ip' that gives the initial values used
      in the ML estimation with discrete characters (see the examples
      in ?ace). This function now returns a matrix giving the indices
      of the estimated rates when analysing discrete characters.

    o nodelabels() and tiplabels() have a new argument `pie' to
      represent proportions, with any number of categories, as
      piecharts. The use of the option `thermo' has been improved:
      there is now no limitation on the number of categories.


BUG FIXES

    o mlphylo() did not work with more than two partitions: this is
      fixed.

    o root() failed if the proposed outgroup was already an outgroup
      in the tree: this is fixed.

    o The `col' argument in nodelabels() and tiplabels() was not
      correctly passed when `text' was used: this is fixed.

    o Two bugs were fixed in mlphylo(): parameters were not always
      correctly output, and the estimation failed in some cases.

    o plot.phylo() was stuck when given a tree with a single tip: this
      is fixed and a message error is now returned.

    o An error was corrected in the help page of gammaStat regarding
      the calculation of P-values.

    o Using gls() could crash R when the number of species in the tree
      and in the variables were different: this is fixed.



                CHANGES IN APE VERSION 1.8-2


NEW FEATURES

    o The new function mlphylo() fits a phylogenetic tree by maximum
      likelihood from DNA sequences. Its companion function DNAmodel()
      is used to define the substitution model which may include
      partitioning. There are methods for logLik(), deviance(), and
      AIC(), and the summary() method has been extended to display in
      a friendly way the results of this model fitting. Currently, the
      functionality is limited to estimating the substitution and
      associated parameters and computing the likelihood.

    o The new function drop1.compar.gee (used as, e.g., drop1(m))
      tests for single effects in GEE-based comparative method. A
      warning message is printed if there is not enough degrees of
      freedom.


BUG FIXES

    o An error message was sometimes issued by plot.multi.tree(),
      though with no consequence.



                CHANGES IN APE VERSION 1.8-1


NEW FEATURES

    o There is a new plot method for lists of trees (objects of class
      "multi.tree"): it calls plot.phylo() internally and is
      documented on the same help page.


BUG FIXES

    o A bug was fixed in the C code that analyzes bipartitions: this
      has impact on several functions like prop.part, prop.clades,
      boot.phylo, or consensus.

    o root() did not work correctly when the specified outgroup had
      more than one element: this is fixed.

    o dist.dna() sometimes returned a warning inappropriately: this
      has been corrected.

    o If the distance object given to nj() had no rownames, nj()
      returned a tree with no tip labels: it now returns tips labelled
      "1", "2", ..., corresponding to the row numbers.


OTHER CHANGES

    o nj() has been slightly changed so that tips with a zero distance
      are first aggregated with zero-lengthed branches; the usual NJ
      procedure is then performed on a distance matrix without 0's.



                CHANGES IN APE VERSION 1.8


NEW FEATURES

    o The new function chronopl() estimates dates using the penalized
      likelihood method by Sanderson (2002; Mol. Biol. Evol., 19:101).

    o The new function consensus() calculates the consensus tree of a
      list of trees.

    o The new function evolve.phylo() simulates the evolution of
      continuous characters along a phylogeny under a Brownian model.

    o The new plot method for objects of class "ancestral" displays a
      tree together with ancestral values, as returned by the above
      function.

    o The new function as.phylo.formula() returns a phylogeny from a
      set of nested taxonomic variables given as a formula.

    o The new function read.caic() reads trees in CAIC format.

    o The new function tiplabels() allows to add labels to the tips
      of a tree using text or plotting symbols in a flexible way.

    o The new function unroot() unroots a phylogeny.

    o multi2di() has a new option, `random', which specifies whether
      to resolve the multichotomies randomly (the default) or not.

    o prop.part() now returns an object of class "prop.part" for which
      there are print (to display a partition in a more friendly way)
      and summary (to extract the numbers) methods.

    o plot.phylo() has a new option, `show.tip.label', specifying
      whether to print the labels of the tips. The default is TRUE.

    o The code of nj() has been replaced by a faster C code: it is now
      about 10, 25, and 40 times faster for 50, 100, and 200 taxa,
      respectively.

    o write.nexus() now writes whether a tree is rooted or not.


BUG FIXES

    o Two bugs have been fixed in root(): unrooted trees are now
      handled corretly, and node labels are now output normally.

    o A bug was fixed in phymltest(): the executable couldn't be found
      in some cases.

    o Three bug have been fixed in ace(): computing the likelihood of
      ancestral states of discrete characters failed, custom models
      did not work, and the function failed with a null gradient (a
      warning message is now returned; this latter bug was also
      present in yule.cov() as well and is now fixed).

    o pic() hanged out when missing data were present: a message error
      is now returned.

    o A small bug was fixed in dist.dna() where the gamma correction
      was not always correctly dispatched.

    o plot.phylo() plotted correctly the root edge only when the tree
      was plotted rightwards: this works now for all directions.


OTHER CHANGES

    o dist.taxo() has been renamed as weight.taxo().

    o dist.phylo() has been replaced by the method cophenetic.phylo().

    o Various error and warning messages have been improved.



                CHANGES IN APE VERSION 1.7
NEW FEATURES

    o The new function ace() estimates ancestral character states for
      continuous characters (with ML, GLS, and contrasts methods), and
      discrete characters (with ML only) for any number of states.

    o The new function compar.ou() fits the Ornstein-Uhlenbeck model
      of directional evolution for continuous characters. The user
      specifies the node(s) of the tree where the character optimum
      changes.

    o The new function is.rooted() tests whether a tree (of class
      "phylo") is rooted.

    o The new function rcoal() generates random ultrametric trees with
      the possibility to specify the function that generates the
      inter-nodes distances.

    o The new function mrca() gives for all pairs of tips in a tree
      (and optionally nodes too) the most recent common ancestor.

    o nodelabels() has a new option `thermo' to plot proportions (up
      to three classes) on the nodes of a tree.

    o rtree() has been improved: it can now generate rooted or
      unrooted trees, and the mathematical function that generates the
      branch lengths may be specified by the user. The tip labels may
      be given directly in the call to rtree. The limit cases (n = 2,
      3) are now handled correctly.

    o dist.topo() has a new argument `method' with two choices: "PH85"
      for Penny and Henny's method (already available before and now
      the default), and "BHV01" for the geometric distance by Billera
      et al. (2001, Adv. Appl. Math. 27:733).

    o write.tree() has a new option, `digits', which specifies the
      number of digits to be printed in the Newick tree. By default
      digits = 10. The numbers are now always printed in decimal form
      (i.e., 1.0e-1 is now avoided).

    o dist.dna() can now compute the raw distances between pairs of
      DNA sequences by specifying model = "raw".

    o dist.phylo() has a new option `full' to possibly compute the
      distances among all tips and nodes of the tree. The default if
      `full = FALSE'.


BUG FIXES

    o Several bugs were fixed in all.equal.phylo().

    o dist.dna() did not handle correctly gaps ("-") in alignments:
      they are now considered as missing data.

    o rotate() did not work if the tips were not ordered: this is
      fixed.

    o mantel.test() returned NA in some special cases: this is fixed
      and the function has been improved and is now faster.

    o A bug was fixed in diversi.gof() where the calculation of A² was
      incorrect.

    o cherry() did not work correctly under some OSs (mainly Linux):
      this is fixed.

    o is.binary.tree() has been modified so that it works with both
      rooted and unrooted trees.

    o The documentation of theta.s() was not correct: this has been
      fixed.

    o plot.mst() did not work correctly: this is fixed.



                CHANGES IN APE VERSION 1.6


NEW FEATURES

    o The new function dist.topo() computes the topological distances
      between two trees.

    o The new function boot.phylo() performs a bootstrap analysis on
      phylogeny estimation.

    o The new functions prop.part() and prop.clades() analyse
      bipartitions from a series of trees.


OTHER CHANGES

    o read.GenBank() now uses the EFetch utility of NCBI instead of
      the usual Web interface: it is now much faster (e.g., 12 times
      faster to retrieve 8 sequences, 37 times for 60 sequences).


BUG FIXES

    o Several bugs were fixed in read.dna().

    o Several bugs were fixed in diversi.time().

    o is.binary.tree() did not work correctly if the tree has no edge
      lengths: this is fixed.

    o drop.tip() did not correctly propagated the `node.label' of a
      tree: this is fixed.



                CHANGES IN APE VERSION 1.5


NEW FEATURES

    o Two new functions, as.matching.phylo() and as.phylo.matching(),
      convert objects between the classes "phylo" and "matching". The
      latter implements the representation of binary trees introduced by
      Diaconis and Holmes (1998; PNAS 95:14600). The generic function
      as.matching() has been introduced as well.

    o Two new functions, multi2di() and di2multi(), allow to resolve
      and collapse multichotomies with branches of length zero.

    o The new function nuc.div() computes the nucleotide diversity
      from a sample a DNA sequences.

    o dist.dna() has been completely rewritten with a much faster
      (particularly for large data sets) C code. Eight models are
      available: JC69, K80, F81, K81, F84, T92, TN93, and GG95 (the
      option `method' has been renamed `model'). Computation of variance
      is available for all models. A gamma-correction is possible for
      JC69, K80, F81, and TN93. There is a new option, pairwise.deletion,
      to remove sites with missing data on a pairwise basis. The option
      `GCcontent' has been removed.

    o read.GenBank() has a new option (species.names) which specifies
      whether to return the species names of the organisms in addition
      to the accession numbers of the sequences (this is the default
      behaviour).

    o write.nexus() can now write several trees in the same NEXUS file.

    o drop.tip() has a new option `root.edge' that allows to specify the
      new root edge if internal branches are trimmed.


BUG FIXES

    o as.phylo.hclust() failed if some labels had parentheses: this
      is fixed.

    o Several bugs were fixed in all.equal.phylo(). This function now
      returns the logical TRUE if the trees are identical but with
      different representations (a report was printed previously).

    o read.GenBank() did not correctly handle ambiguous base codes:
      this is fixed.


OTHER CHANGES

    o birthdeath() now returns an object of class "birthdeath" for
      which there is a print method.



                CHANGES IN APE VERSION 1.4


NEW FEATURES

    o The new function nj() performs phylogeny estimation with the
      neighbor-joining method of Saitou and Nei (1987; Mol. Biol.
      Evol., 4:406).

    o The new function which.edge() identifies the edges of a tree
      that belong to a group specified as a set of tips.

    o The new function as.phylo.phylog() converts an object of class
      "phylog" (from the package ade4) into an object of class
      "phylo".

    o The new function axisPhylo() draws axes on the side of a
      phylogeny plot.

    o The new function howmanytrees() calculates the number of trees
      in different cases and giving a number of tips.

    o write.tree() has a new option `multi.line' (TRUE by default) to
      write a Newick tree on several lines rather than on a single
      line.

    o The functionalities of zoom() have been extended. Several
      subtrees can be visualized at the same time, and they are marked
      on the main tree with colors. The context of the subtrees can be
      marked with the option `subtree' (see below).

    o drop.tip() has a new option `subtree' (FALSE by default) which
      specifies whether to output in the tree how many tips have been
      deleted and where.

    o The arguments of add.scale.bar() have been redefined and have
      now default values (see ?add.scale.bar for details). This
      function now works even if the plotted tree has no edge length.

    o plot.phylo() can now plot radial trees, but this does not take
      edge lengths into account.

    o In plot.phylo() with `type = "phylogram"', if the values of
      `edge.color' and `edge.width' are identical for sister-branches,
      they are propagated to the vertical line that link them.


BUG FIXES

    o Repeated calls to as.phylo.hclust() or as.hclust.phylo() made R
      crashing. This is fixed.

    o In plot.phylo(), the options `edge.color' and `edge.width' are
      now properly recycled; their default values are now "black" and
      1, respectively.

    o A bug has been fixed in write.nexus().


OTHER CHANGES

    o The function node.depth.edgelength() has been removed and
      replaced by a C code.



                CHANGES IN APE VERSION 1.3-1


NEW FEATURES

    o The new function nodelabels() allows to add labels to the nodes
      of a tree using text or plotting symbols in a flexible way.

    o In plot.phylo() the arguments `x.lim' and `y.lim' can now be two
      numeric values specifying the lower and upper limits on the x-
      and y-axes. This allows to leave some space on any side of the
      tree. If a single value is given, this is taken as the upper
      limit (as before).



                CHANGES IN APE VERSION 1.3


NEW FEATURES

    o The new function phymltest() calls the software PHYML and fits
      28 models of DNA sequence evolution. There are a print method to
      display likelihood and AIC values, a summary method to compute
      the hierarchical likelihood ratio tests, and a plot method to
      display graphically the AIC values of each model.

    o The new function yule.cov() fits the Yule model with covariates,
      a model where the speciation rate is affected by several species
      traits through a generalized linear model. The parameters are
      estimated by maximum likelihood.

    o Three new functions, corBrownian(), corGrafen(), and
      corMartins(), compute the expected correlation structures among
      species given a phylogeny under different models of evolution.
      These can be used for GLS comparative phylogenetic methods (see
      the examples). There are coef() and corMatrix() methods and an
      Initialize.corPhyl() function associated.

    o The new function compar.cheverud() implements Cheverud et al.'s
      (1985; Evolution 39:1335) phylogenetic comparative method.

    o The new function varcomp() estimates variance components; it has
      a plot method.

    o Two new functions, panel.superpose.correlogram() and
      plot.correlogramList(), allow to plot several phylogenetic
      correlograms.

    o The new function node.leafnumber() computes the number of leaves
      of a subtree defined by a particular node.

    o The new function node.sons() gets all tags of son nodes from a
      given parent node.

    o The new function compute.brlen() computes the branch lengths of
      a tree according to a specified method.

    o plot.phylo() has three new options: "cex" controls the size of
      the (tip and node) labels (thus it is no more needed to change
      the global graphical parameter), "direction" which allows to
      plot the tree rightwards, leftwards, upwards, or downwards, and
      "y.lim" which sets the upper limit on the y-axis.


BUG FIXES

    o Some functions which try to match tip labels and names of
      additional data (e.g. vector) are likely to fail if there are
      typing or syntax errors. If both series of names do not perfectly
      match, they are ignored and a warning message is now issued.
      These functions are bd.ext, compar.gee, pic. Their help pages
      have been clarified on this point.



                CHANGES IN APE VERSION 1.2-7


NEW FEATURES

    o The new function root() reroots a phylogenetic tree with respect
      to a specified outgroup.

    o The new function rotate() rotates an internal branch of a tree.

    o In plot.phylo(), the new argument "lab4ut" (labels for unrooted
      trees) controls the display of the tip labels in unrooted trees.
      This display has been greatly improved: the tip labels are now not
      expected to overlap with the tree (particularly if lab4ut =
      "axial"). In all cases, combining appropriate values of "lab4ut"
      and the font size (via "par(cex = )") should result in readable
      unrooted trees. See ?plot.phylo for some examples.

    o In drop.tip(), the argument `tip' can now be numeric or character.


BUG FIXES

    o drop.tip() did not work correctly with trees with no branch
      lengths: this is fixed.

    o A bug in plot.phylo(..., type = "unrooted") made some trees being
      plotted with some line crossings: this is now fixed.



                CHANGES IN APE VERSION 1.2-6


NEW FEATURES

    o Six new functions (Moran.I, correlogram.formula, discrete.dist,
      correlogram.phylo, dist.taxo, plot.correlogram) have been added
      to implement comparative methods with an autocorrelation approach.

    o A new data set describing some life history traits of Carnivores
      has been included.


BUG FIXES

    o A fix was made on mcmc.popsize() to conform to R 2.0.0.


OTHER CHANGES

    o When plotting a tree with plot.phylo(), the new default of the
      option `label.offset' is now 0, so the labels are always visible.



                CHANGES IN APE VERSION 1.2-5


NEW FEATURES

    o The new function bd.ext() fits a birth-death model with combined
      phylogenetic and taxonomic data, and estimates the corresponding
      speciation and extinction rates.


OTHER CHANGES

    o The package gee is no more required by ape but only suggested
      since only the function compar.gee() calls gee.



                CHANGES IN APE VERSION 1.2-4


NEW FEATURES

    o Four new functions (mcmc.popsize, extract.popsize, plot.popsize,
      and lines.popsize) implementing a new approach for inferring the
      demographic history from genealogies using a reversible jump
      MCMC have been introduced.

    o The unit of time in the skyline plot and in the new plots can
      now be chosen to be actual years, rather than substitutions.



                CHANGES IN APE VERSION 1.2-3


NEW FEATURES

    o The new function rtree() generates a random binary tree with or
      without branch lengths.

    o Two new functions for drawing lineages-through-time (LTT) plots
      are provided: ltt.lines() adds a LTT curve to an existing plot,
      and mltt.plot() does a multiple LTT plot giving several trees as
      arguments (see `?ltt.plot' for details).


BUG FIXES

    o Some taxon names made R crashing when calling as.phylo.hclust():
      this is fixed.

    o dist.dna() returned an error with two identical DNA sequences
      (only using the Jukes-Cantor method returned 0): this is fixed.


OTHER CHANGES

    o The function dist.phylo() has been re-written using a different
      algorithm: it is now about four times faster.

    o The code of branching.times() has been improved: it is now about
      twice faster.



                CHANGES IN APE VERSION 1.2-2


NEW FEATURES

    o The new function seg.sites() finds the segregating sites in a
      sample of DNA sequences.


BUG FIXES

    o A bug introduced in read.tree() and in read.nexus() with version
      1.2-1 was fixed.

    o A few errors were corrected and a few examples were added in the
      help pages.



                CHANGES IN APE VERSION 1.2-1


NEW FEATURES

    o plot.phylo() can now draw the edge of the root of a tree if it
      has one (see the new option `root.edge', its default is FALSE).


BUG FIXES

    o A bug was fixed in read.nexus(): files with semicolons inside
      comment blocks were not read correctly.

    o The behaviour of read.tree() and read.nexus() was corrected so
      that tree files with badly represented root edges (e.g., with
      an extra pair of parentheses, see the help pages for details)
      are now correctly represented in the object of class "phylo";
      a warning message is now issued.



                CHANGES IN APE VERSION 1.2


NEW FEATURES

    o plot.phylo() has been completely re-written and offers several
      new functionalities. Three types of trees can now be drawn:
      phylogram (as previously), cladogram, and unrooted tree; in
      all three types the branch lengths can be drawn using the edge
      lengths of the phylogeny or not (e.g., if the latter is absent).
      The vertical position of the nodes can be adjusted with two
      choices (see option `node.pos'). The code has been re-structured,
      and two new functions (potentially useful for developpers) are
      documented separately: node.depth.edgelength() and node.depth();
      see the respective help pages for details.

    o The new function zoom() allows to explore very large trees by
      focusing on a small portion of it.

    o The new function yule() fits by maximum likelihood the Yule model
      (birth-only process) to a phylogenetic tree.

    o Support for writing DNA sequences in FASTA format has been
      introduced in write.dna() (support for reading sequences in
      this format was introduced in read.dna() in version 1.1-2).
      The function has been completely re-written, fixing some bugs
      (see below); the default behaviour is no more to display the
      sequences on the standard output. Several options have been
      introduced to control the sequence printing in a flexible
      way. The help page has been extended.

    o A new data set is included: a supertree of bats in NEXUS format.


BUG FIXES

    o In theta.s(), the default of the option `variance' has
      been changed to `FALSE' (as was indicated in the help page).

    o Several bugs were fixed in the code of all.equal.phylo().

    o Several bugs were fixed in write.dna(), particularly this
      function did not work with `format = "interleaved"'.

    o Various errors were corrected in the help pages.


OTHER CHANGES

    o The argument names of as.hclust.phylo() have been changed
      from "(phy)" to "(x, ...)" to conform to the definition of
      the corresponding generic function.

    o gamma.stat() has been renamed gammaStat() to avoid confusion
      since gamma() is a generic function.



                CHANGES IN APE VERSION 1.1-3


BUG FIXES

    o base.freq() previously did not return a value of 0 for
      bases absent in the data (e.g., a vector of length 3 was
      returned if one base was absent). This is now fixed (a
      vector of length 4 is always returned).

    o Several bugs were fixed in read.nexus(), including that this
      function did not work in this absence of a "TRANSLATE"
      command in the NEXUS file, and that the commands were
      case-sensitive.



                CHANGES IN APE VERSION 1.1-2


NEW FEATURES

    o The Tamura and Nei (1993) model of DNA distance is now implemented
      in dist.dna(): five models are now available in this function.

    o A new data set is included: a set of 15 sequences of the
      cytochrome b mitochondrial gene of the woodmouse (Apodemus
      sylvaticus).


BUG FIXES

    o A bug in read.nexus() was fixed.

    o read.dna() previously did not work correctly in most cases.
      The function has been completely re-written and its help page
      has been considerably extended (see ?read.dna for details).
      Underscores (_) in taxon names are no more replaced with
      spaces (this behaviour was undocumented).

    o A bug was fixed in write.dna().



                CHANGES IN APE VERSION 1.1-1


BUG FIXES

    o A bug in read.tree() introduced in APE 1.1 was fixed.

    o A bug in compar.gee() resulted in an error when trying to fit
      a model with `family = "binomial"'. This is now fixed.



                CHANGES IN APE VERSION 1.1


NEW FEATURES

    o The Klastorin (1982) method as suggested by Misawa and Tajima
      (2000, Mol. Biol. Evol. 17:1879-1884) for classifying genes
      on the basis of phylogenetic trees has been implemented (see
      the function klastorin()).

    o Functions have been added to convert APE's "phylo" objects in
      "hclust" cluster objects and vice versa (see the help page of
      as.phylo for details).

    o Three new functions, ratogram(), chronogram() and NPRS.criterion(),
      are introduced for the estimation of absolute evolutionary rates
      (ratogram) and dated clock-like trees (chronogram) from
      phylogenetic trees using the non-parametric rate smoothing approach
      by MJ Sanderson (1997, Mol. Biol. Evol. 14:1218-1231).

    o A summary method is now provided printing a summary information on a
      phylogenetic tree with, for instance, `summary(tree)'.

    o The behaviour of read.tree() was changed so that all spaces and
      tabulations in tree files are now ignored. Consequently, spaces in tip
      labels are no more allowed. Another side effect is that read.nexus()
      now does not replace the underscores (_) in tip labels with spaces
      (this behaviour was undocumented).

    o The function plot.phylo() has a new option (`underscore') which
      specifies whether the underscores in tip labels should be written on
      the plot as such or replaced with spaces (the default).

    o The function birthdeath() now computes 95% confidence intervals of
      the estimated parameters using profile likelihood.

    o Three new data sets are included: a gene tree estimated from 36
      landplant rbcL sequences, a gene tree estimated from 32 opsin
      sequences, and a gene tree for 50 BRCA1 mammalian sequences.


BUG FIXES

    o A bug was fixed in dist.gene() where nothing was returned.

    o A bug in plot.mst() was fixed.

    o A bug in vcv.phylo() resulted in false correlations when the
      option `cor = TRUE' was used (now fixed).



                CHANGES IN APE VERSION 1.0


NEW FEATURES

    o Two new functions, read.dna() and write.dna(), read/write in a file
      DNA sequences in interleaved or in sequential format.

    o Two new functions, read.nexus() and write.nexus(), read/write trees
      in a NEXUS file.

    o The new function bind.tree() allows to bind two trees together,
      possibly handling root edges to give internal branches.

    o The new function drop.tip() removes the tips in a phylogenetic tree,
      and trims (or not) the corresponding internal branches.

    o The new function is.ultrametric() tests if a tree is ultrametric.

    o The function plot.phylo() has more functionalities such as drawing the
      branches with different colours and/or different widths, showing the
      node labels, controling the position and font of the labels, rotating
      the labels, and controling the space around the plot.

    o The function read.tree() can now read trees with no branch length,
      such as "(a,b),c);". Consequently, the element `edge.length' in
      objects of class "phylo" is now optional.

    o The function write.tree() has a new default behaviour: if the default
      for the option `file' is used (i.e. file = ""), then a variable of
      mode character containing the tree in Newick format is returned which
      can thus be assigned (e.g., tree <- write.tree(phy)).

    o The function read.tree() has a new argument `text' which allows
      to read the tree in a variable of mode character.

    o A new data set is included: the phylogenetic relationships among
      the orders of birds from Sibley and Ahlquist (1990).



                CHANGES IN APE VERSION 0.2-1


BUG FIXES

    o Several bugs were fixed in the help pages.



                CHANGES IN APE VERSION 0.2


NEW FEATURES

    o The function write.tree() writes phylogenetic trees (objects of class
      "phylo") in an ASCII file using the Newick parenthetic format.

    o The function birthdeath() fits a birth-death model to branching times
      by maximum likelihood, and estimates the corresponding speciation and
      extinction rates.

    o The function scale.bar() adds a scale bar to a plot of a phylogenetic
      tree.

    o The function is.binary.tree() tests whether a phylogeny is binary.

    o Two generic functions, coalescent.intervals() and collapsed.intervals(),
      as well as some methods are introduced.

    o Several functions, including some generics and methods, for computing
      skyline plot estimates (classic and generalized) of effective
      population size through time are introduced and replace the function
      skyline.plot() in version 0.1.

    o Two data sets are now included: the phylogenetic relationships among
      the families of birds from Sibley and Ahlquist (1990), and an
      estimated clock-like phylogeny of HIV sequences sampled in the
      Democratic Republic of Congo.


DEPRECATED & DEFUNCT

    o The function skyline.plot() in ape 0.1 has been deprecated and
      replaced by more elaborate functions (see above).


BUG FIXES

    o Two important bugs were fixed in plot.phylo(): phylogenies with
      multichotomies not at the root or not with only terminal branches,
      and phylogenies with a single node (i.e. only terminal branches)
      did not plot. These trees should be plotted correctly now.

    o Several bugs were fixed in diversi.time() in the computation of
      AICs and LRTs.

    o Various errors were corrected in the help pages.