--- /dev/null
+#!/usr/bin/env ruby
+
+# Copyright (C) 2007-2012 Martin A. Hansen.
+
+# This program is free software; you can redistribute it and/or
+# modify it under the terms of the GNU General Public License
+# as published by the Free Software Foundation; either version 2
+# of the License, or (at your option) any later version.
+
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
+
+# http://www.gnu.org/copyleft/gpl.html
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+# This program is part of the Biopieces framework (www.biopieces.org).
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+# HMMER search sequences in the stream against a specified database.
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+require 'pp'
+require 'maasha/biopieces'
+require 'maasha/fasta'
+
+class Hmmer
+ def initialize(output_file, options)
+ @output_file = output_file
+ @options = options
+ @command = []
+ end
+
+ def search_db(query_file)
+ @command << "hmmsearch"
+ @command << "--tblout #{@output_file}"
+ @command << "--cpu #{@options[:cpus]}"
+ @command << @options[:database]
+ @command << query_file
+
+ execute
+ end
+
+ def each
+ File.open(@output_file, "r") do |ios|
+ ios.each_line do |line|
+ puts "line->#{line}"
+ end
+ end
+ end
+
+ private
+
+ # Method to execute a command using a system() call.
+ # The command is composed of bits from the @command variable.
+ def execute
+ @command.unshift "nice -n 19"
+ @command << "> /dev/null 2>&1" unless @options[:verbose]
+
+ command = @command.join(" ")
+ $stderr.puts "Running command: #{command}" if @options[:verbose]
+ system(command)
+ raise "Command failed: #{command}" unless $?.success?
+
+ @command = []
+ end
+end
+
+casts = []
+casts << {:long=>'database', :short=>'d', :type=>'file!', :mandatory=>true, :default=>nil, :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'cpus', :short=>'c', :type=>'uint', :mandatory=>false, :default=>1, :allowed=>nil, :disallowed=>"0"}
+
+options = Biopieces.options_parse(ARGV, casts)
+tmpdir = Biopieces.mktmpdir
+output_file = File.join(tmpdir, "output.tab")
+query_file = File.join(tmpdir, "query.fna")
+
+Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output|
+ Fasta.open(query_file, "w") do |fasta_io|
+ input.each_record do |record|
+ if record[:SEQ_NAME] and record[:SEQ]
+ entry = Seq.new_bp(record)
+
+ fasta_io.puts entry.to_fasta
+ end
+
+ output.puts record
+ end
+ end
+
+ hm = Hmmer.new(output_file, options)
+ hm.search_db(query_file)
+
+ hm.each do |hit|
+ output.puts hit.to_bp
+ end
+end
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+__END__