--- /dev/null
+package Maasha::RestrictEnz;
+
+
+# Copyright (C) 2006-2007 Martin A. Hansen.
+
+# This program is free software; you can redistribute it and/or
+# modify it under the terms of the GNU General Public License
+# as published by the Free Software Foundation; either version 2
+# of the License, or (at your option) any later version.
+
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
+
+# http://www.gnu.org/copyleft/gpl.html
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+# This module contains routines for matching restriction enzyme cleavage sites.
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+use warnings;
+use strict;
+use Data::Dumper;
+
+use Exporter;
+
+use vars qw( @ISA @EXPORT @EXPORT_OK );
+
+@ISA = qw( Exporter ) ;
+
+use Inline ( C => <<'END_C', DIRECTORY => $ENV{ "BP_TMP" } );
+
+/*
+# UIPAC ambiguity codes for nucleotides:
+#
+# http://droog.gs.washington.edu/parc/images/iupac.html
+#
+# ACGTUMRWSYKVHDBN
+# A1000011100011101
+# C0100010011011011
+# G0010001010110111
+# T0001100101101111
+# U0001100101101111
+# M1100000000000000
+# R1010000000000000
+# W1001100000000000
+# S0110000000000000
+# Y0101100000000000
+# K0011100000000000
+# V1110000000000000
+# H1101100000000000
+# D1011100000000000
+# B0111100000000000
+# N1111100000000000
+*/
+
+
+char ambi_match[16][16] = {
+ "1000011100011101",
+ "0100010011011011",
+ "0010001010110111",
+ "0001100101101111",
+ "0001100101101111",
+ "1100000000000000",
+ "1010000000000000",
+ "1001100000000000",
+ "0110000000000000",
+ "0101100000000000",
+ "0011100000000000",
+ "1110000000000000",
+ "1101100000000000",
+ "1011100000000000",
+ "0111100000000000",
+ "1111100000000000"
+};
+
+
+int hash( char c )
+{
+ switch ( toupper( c ) )
+ {
+ case 'A': return 0;
+ case 'C': return 1;
+ case 'G': return 2;
+ case 'T': return 3;
+ case 'U': return 4;
+ case 'M': return 5;
+ case 'R': return 6;
+ case 'W': return 7;
+ case 'S': return 8;
+ case 'Y': return 9;
+ case 'K': return 10;
+ case 'V': return 11;
+ case 'H': return 12;
+ case 'D': return 13;
+ case 'B': return 14;
+ case 'N': return 0;
+ default: return -1;
+ }
+}
+
+
+void scan( char *seq, char *pat, int seq_len, int pat_len )
+{
+ int i;
+
+ Inline_Stack_Vars;
+ Inline_Stack_Reset;
+
+ for ( i = 0; i < seq_len - pat_len; i++ )
+ {
+ if ( match( &seq[ i ], pat, pat_len ) ) {
+ Inline_Stack_Push( sv_2mortal( newSViv( i ) ) );
+ }
+ }
+
+ Inline_Stack_Done;
+}
+
+
+int match( char *seq1, char *seq2, int len )
+{
+ int i = 0;
+ char c1;
+ char c2;
+
+ while ( i < len )
+ {
+ c1 = seq1[ i ];
+ c2 = seq2[ i ];
+
+ if ( ambi_match[ hash( c1 ) ][ hash( c2 ) ] == '0' ) {
+ return 0;
+ }
+
+ i++;
+ }
+
+ return 1;
+}
+
+END_C
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+sub re_scan
+{
+ my ( $seq, # sequence to scan
+ $re, # hashref with RE info
+ ) = @_;
+
+ my ( @matches );
+
+ @matches = scan( $seq, $re->{ "pattern" }, length $seq, $re->{ "len" } );
+
+ return wantarray ? @matches: \@matches;
+}
+
+
+sub parse_re_data
+{
+ my ( @lines, $line, @fields, @re_data );
+
+ @lines = <DATA>;
+
+ chomp @lines;
+
+ foreach $line ( @lines )
+ {
+ next if $line =~ /^(#|$)/;
+
+ @fields = split " ", $line;
+
+ push @re_data, {
+ name => $fields[ 0 ],
+ pattern => $fields[ 1 ],
+ len => $fields[ 2 ],
+ ncuts => $fields[ 3 ],
+ blunt => $fields[ 4 ],
+ c1 => $fields[ 5 ],
+ c2 => $fields[ 6 ],
+ c3 => $fields[ 7 ],
+ c4 => $fields[ 8 ],
+ };
+ }
+
+ return wantarray ? @re_data : \@re_data;
+}
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+__DATA__
+
+# From REBASE:
+# ftp://ftp.neb.com/pub/rebase/emboss_e.908
+
+# REBASE version 908 emboss_e.908
+#
+# =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
+# REBASE, The Restriction Enzyme Database http://rebase.neb.com
+# Copyright (c) Dr. Richard J. Roberts, 2009. All rights reserved.
+# =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
+#
+# Rich Roberts Jul 28 2009
+#
+# REBASE enzyme patterns for EMBOSS (embossre.enz)
+#
+# Format:
+#
+# name<ws>pattern<ws>len<ws>ncuts<ws>blunt<ws>c1<ws>c2<ws>c3<ws>c4
+#
+# Where:
+# name = name of enzyme
+# pattern = recognition site
+# len = length of pattern
+# ncuts = number of cuts made by enzyme
+# Zero represents unknown
+# blunt = true if blunt end cut, false if sticky
+# c1 = First 5' cut
+# c2 = First 3' cut
+# c3 = Second 5' cut
+# c4 = Second 3' cut
+#
+# Examples:
+# AAC^TGG -> 6 2 1 3 3 0 0
+# A^ACTGG -> 6 2 0 1 5 0 0
+# AACTGG -> 6 0 0 0 0 0 0
+# AACTGG(-5/-1) -> 6 2 0 1 5 0 0
+# (8/13)GACNNNNNNTCA(12/7) -> 12 4 0 -9 -14 24 19
+#
+# i.e. cuts are always to the right of the given
+# residue and sequences are always with reference to
+# the 5' strand.
+# Sequences are numbered ... -3 -2 -1 1 2 3 ... with
+# the first residue of the pattern at base number 1.
+#
+#
+
+AanI TTATAA 6 2 1 3 3 0 0
+AarI CACCTGC 7 2 0 11 15 0 0
+AasI GACNNNNNNGTC 12 2 0 7 5 0 0
+AatI AGGCCT 6 2 1 3 3 0 0
+AatII GACGTC 6 2 0 5 1 0 0
+AbsI CCTCGAGG 8 2 0 2 6 0 0
+AccI GTMKAC 6 2 0 2 4 0 0
+AccII CGCG 4 2 1 2 2 0 0
+AccIII TCCGGA 6 2 0 1 5 0 0
+Acc16I TGCGCA 6 2 1 3 3 0 0
+Acc36I ACCTGC 6 2 0 10 14 0 0
+Acc65I GGTACC 6 2 0 1 5 0 0
+AccB1I GGYRCC 6 2 0 1 5 0 0
+AccB7I CCANNNNNTGG 11 2 0 7 4 0 0
+AccBSI CCGCTC 6 2 1 3 3 0 0
+AceIII cagctc 6 2 0 13 17 0 0
+AciI CCGC 4 2 0 1 3 0 0
+AclI AACGTT 6 2 0 2 4 0 0
+AclWI GGATC 5 2 0 9 10 0 0
+AcoI YGGCCR 6 2 0 1 5 0 0
+AcsI RAATTY 6 2 0 1 5 0 0
+AcuI CTGAAG 6 2 0 22 20 0 0
+AcvI CACGTG 6 2 1 3 3 0 0
+AcyI GRCGYC 6 2 0 2 4 0 0
+AdeI CACNNNGTG 9 2 0 6 3 0 0
+AfaI GTAC 4 2 1 2 2 0 0
+AfeI AGCGCT 6 2 1 3 3 0 0
+AfiI CCNNNNNNNGG 11 2 0 7 4 0 0
+AflII CTTAAG 6 2 0 1 5 0 0
+AflIII ACRYGT 6 2 0 1 5 0 0
+AgeI ACCGGT 6 2 0 1 5 0 0
+AgsI TTSAA 5 2 0 3 2 0 0
+AhaIII tttaaa 6 2 1 3 3 0 0
+AhdI GACNNNNNGTC 11 2 0 6 5 0 0
+AhlI ACTAGT 6 2 0 1 5 0 0
+AjiI CACGTC 6 2 1 3 3 0 0
+AjnI CCWGG 5 2 0 -1 5 0 0
+AjuI GAANNNNNNNTTGG 14 4 0 -8 -13 25 20
+AleI CACNNNNGTG 10 2 1 5 5 0 0
+AlfI GCANNNNNNTGC 12 4 0 -11 -13 24 22
+AloI GAACNNNNNNTCC 13 4 0 -8 -13 25 20
+AluI AGCT 4 2 1 2 2 0 0
+AluBI AGCT 4 2 1 2 2 0 0
+AlwI GGATC 5 2 0 9 10 0 0
+Alw21I GWGCWC 6 2 0 5 1 0 0
+Alw26I GTCTC 5 2 0 6 10 0 0
+Alw44I GTGCAC 6 2 0 1 5 0 0
+AlwFI gaaaynnnnnrtg 13 0 0 0 0 0 0
+AlwNI CAGNNNCTG 9 2 0 6 3 0 0
+Ama87I CYCGRG 6 2 0 1 5 0 0
+Aor13HI TCCGGA 6 2 0 1 5 0 0
+Aor51HI AGCGCT 6 2 1 3 3 0 0
+ApaI GGGCCC 6 2 0 5 1 0 0
+ApaBI gcannnnntgc 11 2 0 8 3 0 0
+ApaLI GTGCAC 6 2 0 1 5 0 0
+ApeKI GCWGC 5 2 0 1 4 0 0
+ApoI RAATTY 6 2 0 1 5 0 0
+ApyPI atcgac 6 2 0 26 24 0 0
+AquIII gaggag 6 2 0 26 24 0 0
+AquIV grggaag 7 2 0 26 24 0 0
+ArsI GACNNNNNNTTYG 13 4 0 -9 -14 24 19
+AscI GGCGCGCC 8 2 0 2 6 0 0
+AseI ATTAAT 6 2 0 2 4 0 0
+Asi256I gatc 4 2 0 1 3 0 0
+AsiGI ACCGGT 6 2 0 1 5 0 0
+AsiSI GCGATCGC 8 2 0 5 3 0 0
+AspI GACNNNGTC 9 2 0 4 5 0 0
+Asp700I GAANNNNTTC 10 2 1 5 5 0 0
+Asp718I GGTACC 6 2 0 1 5 0 0
+AspA2I CCTAGG 6 2 0 1 5 0 0
+AspCNI gccgc 5 0 0 0 0 0 0
+AspEI GACNNNNNGTC 11 2 0 6 5 0 0
+AspLEI GCGC 4 2 0 3 1 0 0
+AspS9I GGNCC 5 2 0 1 4 0 0
+AssI AGTACT 6 2 1 3 3 0 0
+AsuI ggncc 5 2 0 1 4 0 0
+AsuII TTCGAA 6 2 0 2 4 0 0
+AsuC2I CCSGG 5 2 0 2 3 0 0
+AsuHPI GGTGA 5 2 0 13 12 0 0
+AsuNHI GCTAGC 6 2 0 1 5 0 0
+AvaI CYCGRG 6 2 0 1 5 0 0
+AvaII GGWCC 5 2 0 1 4 0 0
+AvaIII atgcat 6 0 0 0 0 0 0
+AviII TGCGCA 6 2 1 3 3 0 0
+AvrII CCTAGG 6 2 0 1 5 0 0
+AxyI CCTNAGG 7 2 0 2 5 0 0
+BaeI ACNNNNGTAYC 11 4 0 -11 -16 23 18
+BaeGI GKGCMC 6 2 0 5 1 0 0
+BalI TGGCCA 6 2 1 3 3 0 0
+BamHI GGATCC 6 2 0 1 5 0 0
+BanI GGYRCC 6 2 0 1 5 0 0
+BanII GRGCYC 6 2 0 5 1 0 0
+BanIII ATCGAT 6 2 0 2 4 0 0
+BarI GAAGNNNNNNTAC 13 4 0 -8 -13 25 20
+BasI CCANNNNNTGG 11 2 0 7 4 0 0
+BauI CACGAG 6 2 0 1 5 0 0
+BbeI GGCGCC 6 2 0 5 1 0 0
+Bbr7I gaagac 6 2 0 13 17 0 0
+BbrPI CACGTG 6 2 1 3 3 0 0
+BbsI GAAGAC 6 2 0 8 12 0 0
+BbuI GCATGC 6 2 0 5 1 0 0
+BbvI GCAGC 5 2 0 13 17 0 0
+BbvII gaagac 6 2 0 8 12 0 0
+Bbv12I GWGCWC 6 2 0 5 1 0 0
+BbvCI CCTCAGC 7 2 0 2 5 0 0
+BccI CCATC 5 2 0 9 10 0 0
+Bce83I cttgag 6 2 0 22 20 0 0
+BceAI ACGGC 5 2 0 17 19 0 0
+BcefI acggc 5 2 0 17 18 0 0
+BcgI CGANNNNNNTGC 12 4 0 -11 -13 24 22
+BciVI GTATCC 6 2 0 12 11 0 0
+BclI TGATCA 6 2 0 1 5 0 0
+BcnI CCSGG 5 2 0 2 3 0 0
+BcuI ACTAGT 6 2 0 1 5 0 0
+BdaI TGANNNNNNTCA 12 4 0 -11 -13 24 22
+BetI wccggw 6 2 0 1 5 0 0
+BfaI CTAG 4 2 0 1 3 0 0
+BfiI ACTGGG 6 2 0 11 10 0 0
+BfmI CTRYAG 6 2 0 1 5 0 0
+BfoI RGCGCY 6 2 0 5 1 0 0
+BfrI CTTAAG 6 2 0 1 5 0 0
+BfuI GTATCC 6 2 0 12 11 0 0
+BfuAI ACCTGC 6 2 0 10 14 0 0
+BfuCI GATC 4 2 0 -1 4 0 0
+BglI GCCNNNNNGGC 11 2 0 7 4 0 0
+BglII AGATCT 6 2 0 1 5 0 0
+BinI ggatc 5 2 0 9 10 0 0
+BisI GCNGC 5 2 0 2 3 0 0
+BlnI CCTAGG 6 2 0 1 5 0 0
+BlpI GCTNAGC 7 2 0 2 5 0 0
+BlsI GCNGC 5 2 0 3 2 0 0
+BmcAI AGTACT 6 2 1 3 3 0 0
+Bme18I GGWCC 5 2 0 1 4 0 0
+Bme1390I CCNGG 5 2 0 2 3 0 0
+BmeRI GACNNNNNGTC 11 2 0 6 5 0 0
+BmeT110I CYCGRG 6 2 0 1 5 0 0
+BmgI gkgccc 6 0 0 0 0 0 0
+BmgBI CACGTC 6 2 1 3 3 0 0
+BmgT120I GGNCC 5 2 0 2 3 0 0
+BmiI GGNNCC 6 2 1 3 3 0 0
+BmrI ACTGGG 6 2 0 11 10 0 0
+BmrFI CCNGG 5 2 0 2 3 0 0
+BmsI GCATC 5 2 0 10 14 0 0
+BmtI GCTAGC 6 2 0 5 1 0 0
+BmuI ACTGGG 6 2 0 11 10 0 0
+BoxI GACNNNNGTC 10 2 1 5 5 0 0
+BpiI GAAGAC 6 2 0 8 12 0 0
+BplI GAGNNNNNCTC 11 4 0 -9 -14 24 19
+BpmI CTGGAG 6 2 0 22 20 0 0
+Bpu10I CCTNAGC 7 2 0 2 5 0 0
+Bpu14I TTCGAA 6 2 0 2 4 0 0
+Bpu1102I GCTNAGC 7 2 0 2 5 0 0
+BpuAI GAAGAC 6 2 0 8 12 0 0
+BpuEI CTTGAG 6 2 0 22 20 0 0
+BpuMI CCSGG 5 2 0 2 3 0 0
+BpvUI CGATCG 6 2 0 4 2 0 0
+BsaI GGTCTC 6 2 0 7 11 0 0
+Bsa29I ATCGAT 6 2 0 2 4 0 0
+BsaAI YACGTR 6 2 1 3 3 0 0
+BsaBI GATNNNNATC 10 2 1 5 5 0 0
+BsaHI GRCGYC 6 2 0 2 4 0 0
+BsaJI CCNNGG 6 2 0 1 5 0 0
+BsaMI GAATGC 6 2 0 7 5 0 0
+BsaWI WCCGGW 6 2 0 1 5 0 0
+BsaXI ACNNNNNCTCC 11 4 0 -10 -13 21 18
+BsbI caacac 6 2 0 27 25 0 0
+Bsc4I CCNNNNNNNGG 11 2 0 7 4 0 0
+BscAI gcatc 5 2 0 9 11 0 0
+BscGI cccgt 5 0 0 0 0 0 0
+Bse1I ACTGG 5 2 0 6 4 0 0
+Bse8I GATNNNNATC 10 2 1 5 5 0 0
+Bse21I CCTNAGG 7 2 0 2 5 0 0
+Bse118I RCCGGY 6 2 0 1 5 0 0
+BseAI TCCGGA 6 2 0 1 5 0 0
+BseBI CCWGG 5 2 0 2 3 0 0
+BseCI ATCGAT 6 2 0 2 4 0 0
+BseDI CCNNGG 6 2 0 1 5 0 0
+Bse3DI GCAATG 6 2 0 8 6 0 0
+BseGI GGATG 5 2 0 7 5 0 0
+BseJI GATNNNNATC 10 2 1 5 5 0 0
+BseLI CCNNNNNNNGG 11 2 0 7 4 0 0
+BseMI GCAATG 6 2 0 8 6 0 0
+BseMII CTCAG 5 2 0 15 13 0 0
+BseNI ACTGG 5 2 0 6 4 0 0
+BsePI GCGCGC 6 2 0 1 5 0 0
+BseRI GAGGAG 6 2 0 16 14 0 0
+BseSI GKGCMC 6 2 0 5 1 0 0
+BseXI GCAGC 5 2 0 13 17 0 0
+BseX3I CGGCCG 6 2 0 1 5 0 0
+BseYI CCCAGC 6 2 0 1 5 0 0
+BsgI GTGCAG 6 2 0 22 20 0 0
+Bsh1236I CGCG 4 2 1 2 2 0 0
+Bsh1285I CGRYCG 6 2 0 4 2 0 0
+BshFI GGCC 4 2 1 2 2 0 0
+BshNI GGYRCC 6 2 0 1 5 0 0
+BshTI ACCGGT 6 2 0 1 5 0 0
+BshVI ATCGAT 6 2 0 2 4 0 0
+BsiI cacgag 6 2 0 1 5 0 0
+BsiEI CGRYCG 6 2 0 4 2 0 0
+BsiHKAI GWGCWC 6 2 0 5 1 0 0
+BsiHKCI CYCGRG 6 2 0 1 5 0 0
+BsiSI CCGG 4 2 0 1 3 0 0
+BsiWI CGTACG 6 2 0 1 5 0 0
+BsiYI ccnnnnnnngg 11 2 0 7 4 0 0
+BslI CCNNNNNNNGG 11 2 0 7 4 0 0
+BslFI GGGAC 5 2 0 15 19 0 0
+BsmI GAATGC 6 2 0 7 5 0 0
+BsmAI GTCTC 5 2 0 6 10 0 0
+BsmBI CGTCTC 6 2 0 7 11 0 0
+BsmFI GGGAC 5 2 0 15 19 0 0
+BsnI GGCC 4 2 1 2 2 0 0
+Bso31I GGTCTC 6 2 0 7 11 0 0
+BsoBI CYCGRG 6 2 0 1 5 0 0
+Bsp13I TCCGGA 6 2 0 1 5 0 0
+Bsp19I CCATGG 6 2 0 1 5 0 0
+Bsp24I gacnnnnnntgg 12 4 0 -9 -14 24 19
+Bsp68I TCGCGA 6 2 1 3 3 0 0
+Bsp119I TTCGAA 6 2 0 2 4 0 0
+Bsp120I GGGCCC 6 2 0 1 5 0 0
+Bsp143I GATC 4 2 0 -1 4 0 0
+Bsp1286I GDGCHC 6 2 0 5 1 0 0
+Bsp1407I TGTACA 6 2 0 1 5 0 0
+Bsp1720I GCTNAGC 7 2 0 2 5 0 0
+BspACI CCGC 4 2 0 1 3 0 0
+BspCNI CTCAG 5 2 0 14 12 0 0
+BspDI ATCGAT 6 2 0 2 4 0 0
+BspD6I gactc 5 2 0 9 11 0 0
+BspEI TCCGGA 6 2 0 1 5 0 0
+BspFNI CGCG 4 2 1 2 2 0 0
+BspGI ctggac 6 0 0 0 0 0 0
+BspHI TCATGA 6 2 0 1 5 0 0
+BspLI GGNNCC 6 2 1 3 3 0 0
+BspLU11I acatgt 6 2 0 1 5 0 0
+BspMI ACCTGC 6 2 0 10 14 0 0
+BspMII tccgga 6 2 0 1 5 0 0
+BspNCI ccaga 5 0 0 0 0 0 0
+BspOI GCTAGC 6 2 0 5 1 0 0
+BspPI GGATC 5 2 0 9 10 0 0
+BspQI GCTCTTC 7 2 0 8 11 0 0
+BspTI CTTAAG 6 2 0 1 5 0 0
+BspT104I TTCGAA 6 2 0 2 4 0 0
+BspT107I GGYRCC 6 2 0 1 5 0 0
+BspTNI GGTCTC 6 2 0 7 11 0 0
+BspXI ATCGAT 6 2 0 2 4 0 0
+BsrI ACTGG 5 2 0 6 4 0 0
+BsrBI CCGCTC 6 2 1 3 3 0 0
+BsrDI GCAATG 6 2 0 8 6 0 0
+BsrFI RCCGGY 6 2 0 1 5 0 0
+BsrGI TGTACA 6 2 0 1 5 0 0
+BsrSI ACTGG 5 2 0 6 4 0 0
+BssAI RCCGGY 6 2 0 1 5 0 0
+BssECI CCNNGG 6 2 0 1 5 0 0
+BssHII GCGCGC 6 2 0 1 5 0 0
+BssKI CCNGG 5 2 0 -1 5 0 0
+BssMI GATC 4 2 0 -1 4 0 0
+BssNI GRCGYC 6 2 0 2 4 0 0
+BssNAI GTATAC 6 2 1 3 3 0 0
+BssSI CACGAG 6 2 0 1 5 0 0
+BssT1I CCWWGG 6 2 0 1 5 0 0
+Bst6I CTCTTC 6 2 0 7 10 0 0
+Bst98I CTTAAG 6 2 0 1 5 0 0
+Bst1107I GTATAC 6 2 1 3 3 0 0
+BstACI GRCGYC 6 2 0 2 4 0 0
+BstAFI CTTAAG 6 2 0 1 5 0 0
+BstAPI GCANNNNNTGC 11 2 0 7 4 0 0
+BstAUI TGTACA 6 2 0 1 5 0 0
+BstBI TTCGAA 6 2 0 2 4 0 0
+Bst2BI CACGAG 6 2 0 1 5 0 0
+BstBAI YACGTR 6 2 1 3 3 0 0
+Bst4CI ACNGT 5 2 0 3 2 0 0
+BstC8I GCNNGC 6 2 1 3 3 0 0
+BstDEI CTNAG 5 2 0 1 4 0 0
+BstDSI CCRYGG 6 2 0 1 5 0 0
+BstEII GGTNACC 7 2 0 1 6 0 0
+BstENI CCTNNNNNAGG 11 2 0 5 6 0 0
+BstF5I GGATG 5 2 0 7 5 0 0
+BstFNI CGCG 4 2 1 2 2 0 0
+BstH2I RGCGCY 6 2 0 5 1 0 0
+BstHHI GCGC 4 2 0 3 1 0 0
+BstKTI GATC 4 2 0 3 1 0 0
+BstMAI GTCTC 5 2 0 6 10 0 0
+BstMBI GATC 4 2 0 -1 4 0 0
+BstMCI CGRYCG 6 2 0 4 2 0 0
+BstMWI GCNNNNNNNGC 11 2 0 7 4 0 0
+BstNI CCWGG 5 2 0 2 3 0 0
+BstNSI RCATGY 6 2 0 5 1 0 0
+BstOI CCWGG 5 2 0 2 3 0 0
+BstPI GGTNACC 7 2 0 1 6 0 0
+BstPAI GACNNNNGTC 10 2 1 5 5 0 0
+BstSCI CCNGG 5 2 0 -1 5 0 0
+BstSFI CTRYAG 6 2 0 1 5 0 0
+BstSLI GKGCMC 6 2 0 5 1 0 0
+BstSNI TACGTA 6 2 1 3 3 0 0
+BstUI CGCG 4 2 1 2 2 0 0
+Bst2UI CCWGG 5 2 0 2 3 0 0
+BstV1I GCAGC 5 2 0 13 17 0 0
+BstV2I GAAGAC 6 2 0 8 12 0 0
+BstXI CCANNNNNNTGG 12 2 0 8 4 0 0
+BstX2I RGATCY 6 2 0 1 5 0 0
+BstYI RGATCY 6 2 0 1 5 0 0
+BstZI CGGCCG 6 2 0 1 5 0 0
+BstZ17I GTATAC 6 2 1 3 3 0 0
+Bsu15I ATCGAT 6 2 0 2 4 0 0
+Bsu36I CCTNAGG 7 2 0 2 5 0 0
+BsuRI GGCC 4 2 1 2 2 0 0
+BsuTUI ATCGAT 6 2 0 2 4 0 0
+BtgI CCRYGG 6 2 0 1 5 0 0
+BtgZI GCGATG 6 2 0 16 20 0 0
+BthCI gcngc 5 2 0 4 1 0 0
+BtrI CACGTC 6 2 1 3 3 0 0
+BtsI GCAGTG 6 2 0 8 6 0 0
+BtsCI GGATG 5 2 0 7 5 0 0
+BtuMI TCGCGA 6 2 1 3 3 0 0
+BveI ACCTGC 6 2 0 10 14 0 0
+Cac8I GCNNGC 6 2 1 3 3 0 0
+CaiI CAGNNNCTG 9 2 0 6 3 0 0
+CauII ccsgg 5 2 0 2 3 0 0
+CciI TCATGA 6 2 0 1 5 0 0
+CciNI GCGGCCGC 8 2 0 2 6 0 0
+CdiI catcg 5 2 1 4 4 0 0
+CdpI gcggag 6 2 0 26 24 0 0
+CelII GCTNAGC 7 2 0 2 5 0 0
+CfoI GCGC 4 2 0 3 1 0 0
+CfrI YGGCCR 6 2 0 1 5 0 0
+Cfr9I CCCGGG 6 2 0 1 5 0 0
+Cfr10I RCCGGY 6 2 0 1 5 0 0
+Cfr13I GGNCC 5 2 0 1 4 0 0
+Cfr42I CCGCGG 6 2 0 4 2 0 0
+ChaI gatc 4 2 0 4 -1 0 0
+CjeI ccannnnnngt 11 4 0 -9 -15 26 20
+CjeNII gagnnnnngt 10 0 0 0 0 0 0
+CjePI ccannnnnnntc 12 4 0 -8 -14 26 20
+CjuI caynnnnnrtg 11 0 0 0 0 0 0
+CjuII caynnnnnctc 11 0 0 0 0 0 0
+ClaI ATCGAT 6 2 0 2 4 0 0
+CpoI CGGWCCG 7 2 0 2 5 0 0
+CseI GACGC 5 2 0 10 15 0 0
+CsiI ACCWGGT 7 2 0 1 6 0 0
+CspI CGGWCCG 7 2 0 2 5 0 0
+Csp6I GTAC 4 2 0 1 3 0 0
+Csp45I TTCGAA 6 2 0 2 4 0 0
+CspAI ACCGGT 6 2 0 1 5 0 0
+CspCI CAANNNNNGTGG 12 4 0 -12 -14 24 22
+CstMI aaggag 6 2 0 26 24 0 0
+CviAII CATG 4 2 0 1 3 0 0
+CviJI RGCY 4 2 1 2 2 0 0
+CviKI-1 RGCY 4 2 1 2 2 0 0
+CviQI GTAC 4 2 0 1 3 0 0
+CviRI tgca 4 2 1 2 2 0 0
+DdeI CTNAG 5 2 0 1 4 0 0
+DinI GGCGCC 6 2 1 3 3 0 0
+DpnI GATC 4 2 1 2 2 0 0
+DpnII GATC 4 2 0 -1 4 0 0
+DraI TTTAAA 6 2 1 3 3 0 0
+DraII RGGNCCY 7 2 0 2 5 0 0
+DraIII CACNNNGTG 9 2 0 6 3 0 0
+DraRI caagnac 7 2 0 27 25 0 0
+DrdI GACNNNNNNGTC 12 2 0 7 5 0 0
+DrdII gaacca 6 0 0 0 0 0 0
+DrdIV tacgac 6 2 0 26 24 0 0
+DriI GACNNNNNGTC 11 2 0 6 5 0 0
+DsaI ccrygg 6 2 0 1 5 0 0
+DseDI GACNNNNNNGTC 12 2 0 7 5 0 0
+EaeI YGGCCR 6 2 0 1 5 0 0
+EagI CGGCCG 6 2 0 1 5 0 0
+Eam1104I CTCTTC 6 2 0 7 10 0 0
+Eam1105I GACNNNNNGTC 11 2 0 6 5 0 0
+EarI CTCTTC 6 2 0 7 10 0 0
+EciI GGCGGA 6 2 0 17 15 0 0
+Ecl136II GAGCTC 6 2 1 3 3 0 0
+EclXI CGGCCG 6 2 0 1 5 0 0
+Eco24I GRGCYC 6 2 0 5 1 0 0
+Eco31I GGTCTC 6 2 0 7 11 0 0
+Eco32I GATATC 6 2 1 3 3 0 0
+Eco47I GGWCC 5 2 0 1 4 0 0
+Eco47III AGCGCT 6 2 1 3 3 0 0
+Eco52I CGGCCG 6 2 0 1 5 0 0
+Eco57I CTGAAG 6 2 0 22 20 0 0
+Eco72I CACGTG 6 2 1 3 3 0 0
+Eco81I CCTNAGG 7 2 0 2 5 0 0
+Eco88I CYCGRG 6 2 0 1 5 0 0
+Eco91I GGTNACC 7 2 0 1 6 0 0
+Eco105I TACGTA 6 2 1 3 3 0 0
+Eco130I CCWWGG 6 2 0 1 5 0 0
+Eco147I AGGCCT 6 2 1 3 3 0 0
+EcoHI ccsgg 5 2 0 -1 5 0 0
+EcoICRI GAGCTC 6 2 1 3 3 0 0
+Eco57MI CTGRAG 6 2 0 22 20 0 0
+EcoNI CCTNNNNNAGG 11 2 0 5 6 0 0
+EcoO65I GGTNACC 7 2 0 1 6 0 0
+EcoO109I RGGNCCY 7 2 0 2 5 0 0
+EcoRI GAATTC 6 2 0 1 5 0 0
+EcoRII CCWGG 5 2 0 -1 5 0 0
+EcoRV GATATC 6 2 1 3 3 0 0
+EcoT14I CCWWGG 6 2 0 1 5 0 0
+EcoT22I ATGCAT 6 2 0 5 1 0 0
+EcoT38I GRGCYC 6 2 0 5 1 0 0
+Eco53kI GAGCTC 6 2 1 3 3 0 0
+EgeI GGCGCC 6 2 1 3 3 0 0
+EheI GGCGCC 6 2 1 3 3 0 0
+ErhI CCWWGG 6 2 0 1 5 0 0
+EsaBC3I tcga 4 2 1 2 2 0 0
+EsaSSI gaccac 6 0 0 0 0 0 0
+EspI gctnagc 7 2 0 2 5 0 0
+Esp3I CGTCTC 6 2 0 7 11 0 0
+FaeI CATG 4 2 0 4 -1 0 0
+FaiI YATR 4 2 1 2 2 0 0
+FalI AAGNNNNNCTT 11 4 0 -9 -14 24 19
+FaqI GGGAC 5 2 0 15 19 0 0
+FatI CATG 4 2 0 -1 4 0 0
+FauI CCCGC 5 2 0 9 11 0 0
+FauNDI CATATG 6 2 0 2 4 0 0
+FbaI TGATCA 6 2 0 1 5 0 0
+FblI GTMKAC 6 2 0 2 4 0 0
+FinI gggac 5 0 0 0 0 0 0
+FmuI ggncc 5 2 0 4 1 0 0
+FnuDII cgcg 4 2 1 2 2 0 0
+Fnu4HI GCNGC 5 2 0 2 3 0 0
+FokI GGATG 5 2 0 14 18 0 0
+FriOI GRGCYC 6 2 0 5 1 0 0
+FseI GGCCGGCC 8 2 0 6 2 0 0
+FspI TGCGCA 6 2 1 3 3 0 0
+FspAI RTGCGCAY 8 2 1 4 4 0 0
+FspBI CTAG 4 2 0 1 3 0 0
+Fsp4HI GCNGC 5 2 0 2 3 0 0
+GdiII cggccr 6 2 0 1 5 0 0
+GlaI GCGC 4 2 1 2 2 0 0
+GluI GCNGC 5 2 0 2 3 0 0
+GsaI CCCAGC 6 2 0 5 1 0 0
+GsuI CTGGAG 6 2 0 22 20 0 0
+HaeI wggccw 6 2 1 3 3 0 0
+HaeII RGCGCY 6 2 0 5 1 0 0
+HaeIII GGCC 4 2 1 2 2 0 0
+HaeIV gaynnnnnrtc 11 4 0 -8 -14 25 20
+HapII CCGG 4 2 0 1 3 0 0
+HgaI GACGC 5 2 0 10 15 0 0
+HgiAI gwgcwc 6 2 0 5 1 0 0
+HgiCI ggyrcc 6 2 0 1 5 0 0
+HgiEII accnnnnnnggt 12 0 0 0 0 0 0
+HgiJII grgcyc 6 2 0 5 1 0 0
+HhaI GCGC 4 2 0 3 1 0 0
+Hin1I GRCGYC 6 2 0 2 4 0 0
+Hin1II CATG 4 2 0 4 -1 0 0
+Hin4I GAYNNNNNVTC 11 4 0 -9 -14 24 19
+Hin4II ccttc 5 2 0 11 10 0 0
+Hin6I GCGC 4 2 0 1 3 0 0
+HinP1I GCGC 4 2 0 1 3 0 0
+HincII GTYRAC 6 2 1 3 3 0 0
+HindII GTYRAC 6 2 1 3 3 0 0
+HindIII AAGCTT 6 2 0 1 5 0 0
+HinfI GANTC 5 2 0 1 4 0 0
+HpaI GTTAAC 6 2 1 3 3 0 0
+HpaII CCGG 4 2 0 1 3 0 0
+HphI GGTGA 5 2 0 13 12 0 0
+Hpy8I GTNNAC 6 2 1 3 3 0 0
+Hpy99I CGWCG 5 2 0 5 -1 0 0
+Hpy166II GTNNAC 6 2 1 3 3 0 0
+Hpy178III tcnnga 6 2 0 2 4 0 0
+Hpy188I TCNGA 5 2 0 3 2 0 0
+Hpy188III TCNNGA 6 2 0 2 4 0 0
+HpyAV CCTTC 5 2 0 11 10 0 0
+HpyCH4III ACNGT 5 2 0 3 2 0 0
+HpyCH4IV ACGT 4 2 0 1 3 0 0
+HpyCH4V TGCA 4 2 1 2 2 0 0
+HpyF3I CTNAG 5 2 0 1 4 0 0
+HpyF10VI GCNNNNNNNGC 11 2 0 7 4 0 0
+Hsp92I GRCGYC 6 2 0 2 4 0 0
+Hsp92II CATG 4 2 0 4 -1 0 0
+HspAI GCGC 4 2 0 1 3 0 0
+ItaI GCNGC 5 2 0 2 3 0 0
+KasI GGCGCC 6 2 0 1 5 0 0
+KflI GGGWCCC 7 2 0 2 5 0 0
+KpnI GGTACC 6 2 0 5 1 0 0
+Kpn2I TCCGGA 6 2 0 1 5 0 0
+KspI CCGCGG 6 2 0 4 2 0 0
+Ksp22I TGATCA 6 2 0 1 5 0 0
+Ksp632I ctcttc 6 2 0 7 10 0 0
+KspAI GTTAAC 6 2 1 3 3 0 0
+Kzo9I GATC 4 2 0 -1 4 0 0
+LguI GCTCTTC 7 2 0 8 11 0 0
+LpnI rgcgcy 6 2 1 3 3 0 0
+Lsp1109I GCAGC 5 2 0 13 17 0 0
+LweI GCATC 5 2 0 10 14 0 0
+MabI ACCWGGT 7 2 0 1 6 0 0
+MaeI CTAG 4 2 0 1 3 0 0
+MaeII ACGT 4 2 0 1 3 0 0
+MaeIII GTNAC 5 2 0 -1 5 0 0
+MalI GATC 4 2 1 2 2 0 0
+MaqI crttgac 7 2 0 28 26 0 0
+MauBI CGCGCGCG 8 2 0 2 6 0 0
+MbiI CCGCTC 6 2 1 3 3 0 0
+MboI GATC 4 2 0 -1 4 0 0
+MboII GAAGA 5 2 0 13 12 0 0
+McaTI gcgcgc 6 2 0 4 2 0 0
+McrI cgrycg 6 2 0 4 2 0 0
+MfeI CAATTG 6 2 0 1 5 0 0
+MflI RGATCY 6 2 0 1 5 0 0
+MhlI GDGCHC 6 2 0 5 1 0 0
+MjaIV gtnnac 6 0 0 0 0 0 0
+MlsI TGGCCA 6 2 1 3 3 0 0
+MluI ACGCGT 6 2 0 1 5 0 0
+MluNI TGGCCA 6 2 1 3 3 0 0
+MlyI GAGTC 5 2 1 10 10 0 0
+Mly113I GGCGCC 6 2 0 2 4 0 0
+MmeI TCCRAC 6 2 0 26 24 0 0
+MnlI CCTC 4 2 0 11 10 0 0
+Mph1103I ATGCAT 6 2 0 5 1 0 0
+MreI CGCCGGCG 8 2 0 2 6 0 0
+MroI TCCGGA 6 2 0 1 5 0 0
+MroNI GCCGGC 6 2 0 1 5 0 0
+MroXI GAANNNNTTC 10 2 1 5 5 0 0
+MscI TGGCCA 6 2 1 3 3 0 0
+MseI TTAA 4 2 0 1 3 0 0
+MslI CAYNNNNRTG 10 2 1 5 5 0 0
+MspI CCGG 4 2 0 1 3 0 0
+Msp20I TGGCCA 6 2 1 3 3 0 0
+MspA1I CMGCKG 6 2 1 3 3 0 0
+MspCI CTTAAG 6 2 0 1 5 0 0
+MspR9I CCNGG 5 2 0 2 3 0 0
+MssI GTTTAAAC 8 2 1 4 4 0 0
+MstI tgcgca 6 2 1 3 3 0 0
+MunI CAATTG 6 2 0 1 5 0 0
+MvaI CCWGG 5 2 0 2 3 0 0
+Mva1269I GAATGC 6 2 0 7 5 0 0
+MvnI CGCG 4 2 1 2 2 0 0
+MvrI CGATCG 6 2 0 4 2 0 0
+MwoI GCNNNNNNNGC 11 2 0 7 4 0 0
+NaeI GCCGGC 6 2 1 3 3 0 0
+NarI GGCGCC 6 2 0 2 4 0 0
+NciI CCSGG 5 2 0 2 3 0 0
+NcoI CCATGG 6 2 0 1 5 0 0
+NdeI CATATG 6 2 0 2 4 0 0
+NdeII GATC 4 2 0 -1 4 0 0
+NgoAVIII gacnnnnntga 11 4 0 -13 -15 24 22
+NgoMIV GCCGGC 6 2 0 1 5 0 0
+NhaXI caagrag 7 0 0 0 0 0 0
+NheI GCTAGC 6 2 0 1 5 0 0
+NlaIII CATG 4 2 0 4 -1 0 0
+NlaIV GGNNCC 6 2 1 3 3 0 0
+NlaCI catcac 6 2 0 25 23 0 0
+Nli3877I cycgrg 6 2 0 5 1 0 0
+NmeAIII GCCGAG 6 2 0 27 25 0 0
+NmeDI rccggy 6 4 0 -13 -8 13 18
+NmuCI GTSAC 5 2 0 -1 5 0 0
+NotI GCGGCCGC 8 2 0 2 6 0 0
+NruI TCGCGA 6 2 1 3 3 0 0
+NsbI TGCGCA 6 2 1 3 3 0 0
+NsiI ATGCAT 6 2 0 5 1 0 0
+NspI RCATGY 6 2 0 5 1 0 0
+NspV TTCGAA 6 2 0 2 4 0 0
+NspBII cmgckg 6 2 1 3 3 0 0
+OliI CACNNNNGTG 10 2 1 5 5 0 0
+PabI gtac 4 2 0 3 1 0 0
+PacI TTAATTAA 8 2 0 5 3 0 0
+PaeI GCATGC 6 2 0 5 1 0 0
+PaeR7I CTCGAG 6 2 0 1 5 0 0
+PagI TCATGA 6 2 0 1 5 0 0
+PalAI GGCGCGCC 8 2 0 2 6 0 0
+PasI CCCWGGG 7 2 0 2 5 0 0
+PauI GCGCGC 6 2 0 1 5 0 0
+PceI AGGCCT 6 2 1 3 3 0 0
+PciI ACATGT 6 2 0 1 5 0 0
+PciSI GCTCTTC 7 2 0 8 11 0 0
+PctI GAATGC 6 2 0 7 5 0 0
+PdiI GCCGGC 6 2 1 3 3 0 0
+PdmI GAANNNNTTC 10 2 1 5 5 0 0
+PfeI GAWTC 5 2 0 1 4 0 0
+Pfl23II CGTACG 6 2 0 1 5 0 0
+Pfl1108I tcgtag 6 0 0 0 0 0 0
+PflFI GACNNNGTC 9 2 0 4 5 0 0
+PflMI CCANNNNNTGG 11 2 0 7 4 0 0
+PfoI TCCNGGA 7 2 0 1 6 0 0
+PhoI GGCC 4 2 1 2 2 0 0
+PinAI ACCGGT 6 2 0 1 5 0 0
+PlaDI catcag 6 2 0 27 25 0 0
+PleI GAGTC 5 2 0 9 10 0 0
+Ple19I CGATCG 6 2 0 4 2 0 0
+PmaCI CACGTG 6 2 1 3 3 0 0
+PmeI GTTTAAAC 8 2 1 4 4 0 0
+PmlI CACGTG 6 2 1 3 3 0 0
+PpiI GAACNNNNNCTC 12 4 0 -8 -13 25 20
+PpsI GAGTC 5 2 0 9 10 0 0
+Ppu10I atgcat 6 2 0 1 5 0 0
+Ppu21I YACGTR 6 2 1 3 3 0 0
+PpuMI RGGWCCY 7 2 0 2 5 0 0
+PscI ACATGT 6 2 0 1 5 0 0
+PshAI GACNNNNGTC 10 2 1 5 5 0 0
+PshBI ATTAAT 6 2 0 2 4 0 0
+PsiI TTATAA 6 2 1 3 3 0 0
+Psp03I ggwcc 5 2 0 4 1 0 0
+Psp5II RGGWCCY 7 2 0 2 5 0 0
+Psp6I CCWGG 5 2 0 -1 5 0 0
+Psp1406I AACGTT 6 2 0 2 4 0 0
+Psp124BI GAGCTC 6 2 0 5 1 0 0
+PspCI CACGTG 6 2 1 3 3 0 0
+PspEI GGTNACC 7 2 0 1 6 0 0
+PspGI CCWGG 5 2 0 -1 5 0 0
+PspLI CGTACG 6 2 0 1 5 0 0
+PspN4I GGNNCC 6 2 1 3 3 0 0
+PspOMI GGGCCC 6 2 0 1 5 0 0
+PspOMII cgcccar 7 2 0 27 25 0 0
+PspPI GGNCC 5 2 0 1 4 0 0
+PspPPI RGGWCCY 7 2 0 2 5 0 0
+PspPRI ccycag 6 2 0 21 19 0 0
+PspXI VCTCGAGB 8 2 0 2 6 0 0
+PsrI GAACNNNNNNTAC 13 4 0 -8 -13 25 20
+PssI rggnccy 7 2 0 5 2 0 0
+PstI CTGCAG 6 2 0 5 1 0 0
+PsuI RGATCY 6 2 0 1 5 0 0
+PsyI GACNNNGTC 9 2 0 4 5 0 0
+PteI GCGCGC 6 2 0 1 5 0 0
+PvuI CGATCG 6 2 0 4 2 0 0
+PvuII CAGCTG 6 2 1 3 3 0 0
+RcaI TCATGA 6 2 0 1 5 0 0
+RceI catcgac 7 2 0 27 25 0 0
+RgaI GCGATCGC 8 2 0 5 3 0 0
+RigI GGCCGGCC 8 2 0 6 2 0 0
+RleAI cccaca 6 2 0 18 15 0 0
+RpaB5I cgrggac 7 2 0 27 25 0 0
+RruI TCGCGA 6 2 1 3 3 0 0
+RsaI GTAC 4 2 1 2 2 0 0
+RsaNI GTAC 4 2 0 1 3 0 0
+RseI CAYNNNNRTG 10 2 1 5 5 0 0
+RsrII CGGWCCG 7 2 0 2 5 0 0
+Rsr2I CGGWCCG 7 2 0 2 5 0 0
+SacI GAGCTC 6 2 0 5 1 0 0
+SacII CCGCGG 6 2 0 4 2 0 0
+SalI GTCGAC 6 2 0 1 5 0 0
+SanDI GGGWCCC 7 2 0 2 5 0 0
+SapI GCTCTTC 7 2 0 8 11 0 0
+SaqAI TTAA 4 2 0 1 3 0 0
+SatI GCNGC 5 2 0 2 3 0 0
+SauI cctnagg 7 2 0 2 5 0 0
+Sau96I GGNCC 5 2 0 1 4 0 0
+Sau3AI GATC 4 2 0 -1 4 0 0
+SbfI CCTGCAGG 8 2 0 6 2 0 0
+ScaI AGTACT 6 2 1 3 3 0 0
+SchI GAGTC 5 2 1 10 10 0 0
+SciI ctcgag 6 2 1 3 3 0 0
+ScrFI CCNGG 5 2 0 2 3 0 0
+SdaI CCTGCAGG 8 2 0 6 2 0 0
+SdeAI cagrag 6 2 0 27 25 0 0
+SdeOSI gacnnnnrtga 11 4 0 -12 -14 23 21
+SduI GDGCHC 6 2 0 5 1 0 0
+SecI ccnngg 6 2 0 1 5 0 0
+SelI cgcg 4 2 0 -1 4 0 0
+SetI ASST 4 2 0 4 -1 0 0
+SexAI ACCWGGT 7 2 0 1 6 0 0
+SfaAI GCGATCGC 8 2 0 5 3 0 0
+SfaNI GCATC 5 2 0 10 14 0 0
+SfcI CTRYAG 6 2 0 1 5 0 0
+SfeI ctryag 6 2 0 1 5 0 0
+SfiI GGCCNNNNNGGCC 13 2 0 8 5 0 0
+SfoI GGCGCC 6 2 1 3 3 0 0
+Sfr274I CTCGAG 6 2 0 1 5 0 0
+Sfr303I CCGCGG 6 2 0 4 2 0 0
+SfuI TTCGAA 6 2 0 2 4 0 0
+SgfI GCGATCGC 8 2 0 5 3 0 0
+SgrAI CRCCGGYG 8 2 0 2 6 0 0
+SgrBI CCGCGG 6 2 0 4 2 0 0
+SgrDI CGTCGACG 8 2 0 2 6 0 0
+SgsI GGCGCGCC 8 2 0 2 6 0 0
+SimI gggtc 5 2 0 2 5 0 0
+SinI GGWCC 5 2 0 1 4 0 0
+SlaI CTCGAG 6 2 0 1 5 0 0
+SmaI CCCGGG 6 2 1 3 3 0 0
+SmiI ATTTAAAT 8 2 1 4 4 0 0
+SmiMI CAYNNNNRTG 10 2 1 5 5 0 0
+SmlI CTYRAG 6 2 0 1 5 0 0
+SmoI CTYRAG 6 2 0 1 5 0 0
+SmuI CCCGC 5 2 0 9 11 0 0
+SnaI gtatac 6 0 0 0 0 0 0
+SnaBI TACGTA 6 2 1 3 3 0 0
+SpeI ACTAGT 6 2 0 1 5 0 0
+SphI GCATGC 6 2 0 5 1 0 0
+SplI cgtacg 6 2 0 1 5 0 0
+SpoDI gcggrag 7 0 0 0 0 0 0
+SrfI GCCCGGGC 8 2 1 4 4 0 0
+Sse9I AATT 4 2 0 -1 4 0 0
+Sse232I cgccggcg 8 2 0 2 6 0 0
+Sse8387I CCTGCAGG 8 2 0 6 2 0 0
+Sse8647I aggwcct 7 2 0 2 5 0 0
+SseBI AGGCCT 6 2 1 3 3 0 0
+SsiI CCGC 4 2 0 1 3 0 0
+SspI AATATT 6 2 1 3 3 0 0
+SspDI GGCGCC 6 2 0 1 5 0 0
+SspD5I ggtga 5 2 1 13 13 0 0
+SstI GAGCTC 6 2 0 5 1 0 0
+SstII CCGCGG 6 2 0 4 2 0 0
+Sth132I cccg 4 2 0 8 12 0 0
+Sth302II ccgg 4 2 1 2 2 0 0
+StrI CTCGAG 6 2 0 1 5 0 0
+StsI ggatg 5 2 0 15 19 0 0
+StuI AGGCCT 6 2 1 3 3 0 0
+StyI CCWWGG 6 2 0 1 5 0 0
+StyD4I CCNGG 5 2 0 -1 5 0 0
+SwaI ATTTAAAT 8 2 1 4 4 0 0
+TaaI ACNGT 5 2 0 3 2 0 0
+TaiI ACGT 4 2 0 4 -1 0 0
+TaqI TCGA 4 2 0 1 3 0 0
+TaqII GACCGA 6 2 0 17 15 0 0
+TaqII CACCCA 6 2 0 17 15 0 0
+TasI AATT 4 2 0 -1 4 0 0
+TatI WGTACW 6 2 0 1 5 0 0
+TauI GCSGC 5 2 0 4 1 0 0
+TfiI GAWTC 5 2 0 1 4 0 0
+TliI CTCGAG 6 2 0 1 5 0 0
+Tru1I TTAA 4 2 0 1 3 0 0
+Tru9I TTAA 4 2 0 1 3 0 0
+TscAI CASTG 5 2 0 7 -3 0 0
+TseI GCWGC 5 2 0 1 4 0 0
+TsoI TARCCA 6 2 0 17 15 0 0
+Tsp45I GTSAC 5 2 0 -1 5 0 0
+Tsp509I AATT 4 2 0 -1 4 0 0
+Tsp4CI acngt 5 2 0 3 2 0 0
+TspDTI ATGAA 5 2 0 16 14 0 0
+TspEI AATT 4 2 0 -1 4 0 0
+TspGWI ACGGA 5 2 0 16 14 0 0
+TspMI CCCGGG 6 2 0 1 5 0 0
+TspRI CASTG 5 2 0 7 -3 0 0
+TssI gagnnnctc 9 0 0 0 0 0 0
+TstI CACNNNNNNTCC 12 4 0 -9 -14 24 19
+TsuI gcgac 5 0 0 0 0 0 0
+Tth111I GACNNNGTC 9 2 0 4 5 0 0
+Tth111II caarca 6 2 0 17 15 0 0
+UbaF9I tacnnnnnrtgt 12 0 0 0 0 0 0
+UbaF11I tcgta 5 0 0 0 0 0 0
+UbaF12I ctacnnngtc 10 0 0 0 0 0 0
+UbaF13I gagnnnnnnctgg 13 0 0 0 0 0 0
+UbaF14I ccannnnntcg 11 0 0 0 0 0 0
+UbaPI cgaacg 6 0 0 0 0 0 0
+UnbI ggncc 5 2 0 -1 5 0 0
+Van91I CCANNNNNTGG 11 2 0 7 4 0 0
+Vha464I CTTAAG 6 2 0 1 5 0 0
+VneI GTGCAC 6 2 0 1 5 0 0
+VpaK11AI ggwcc 5 2 0 -1 5 0 0
+VpaK11BI GGWCC 5 2 0 1 4 0 0
+VspI ATTAAT 6 2 0 2 4 0 0
+XagI CCTNNNNNAGG 11 2 0 5 6 0 0
+XapI RAATTY 6 2 0 1 5 0 0
+XbaI TCTAGA 6 2 0 1 5 0 0
+XceI RCATGY 6 2 0 5 1 0 0
+XcmI CCANNNNNNNNNTGG 15 2 0 8 7 0 0
+XhoI CTCGAG 6 2 0 1 5 0 0
+XhoII RGATCY 6 2 0 1 5 0 0
+XmaI CCCGGG 6 2 0 1 5 0 0
+XmaIII cggccg 6 2 0 1 5 0 0
+XmaCI CCCGGG 6 2 0 1 5 0 0
+XmaJI CCTAGG 6 2 0 1 5 0 0
+XmiI GTMKAC 6 2 0 2 4 0 0
+XmnI GAANNNNTTC 10 2 1 5 5 0 0
+XspI CTAG 4 2 0 1 3 0 0
+ZraI GACGTC 6 2 1 3 3 0 0
+ZrmI AGTACT 6 2 1 3 3 0 0
+Zsp2I ATGCAT 6 2 0 5 1 0 0
projects / biopieces.git / commitdiff