-#!/usr/bin/env perl
+#!/usr/bin/env perl -w
+
+# Copyright (C) 2007-2009 Martin A. Hansen.
+
+# This program is free software; you can redistribute it and/or
+# modify it under the terms of the GNU General Public License
+# as published by the Free Software Foundation; either version 2
+# of the License, or (at your option) any later version.
+
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+# GNU General Public License for more details.
+
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
+
+# http://www.gnu.org/copyleft/gpl.html
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+# Calculate the bit score for each position based on an alignment in the stream.
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
-use warnings;
use strict;
+use Maasha::Biopieces;
+use Maasha::Seq;
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+my ( $run_time_beg, $run_time_end, $options, $in, $out, $record, $count, $i, $res, $type, $bit_max, %freq_hash, $bit_height, $bit_diff );
+
+$options = Maasha::Biopieces::parse_options();
+
+$in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
+$out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
+
+$count = 0;
+
+while ( $record = Maasha::Biopieces::get_record( $in ) )
+{
+ if ( $record->{ "SEQ" } )
+ {
+ $type = Maasha::Seq::seq_guess_type( $record->{ "SEQ" } ) if not $type;
+
+ for ( $i = 0; $i < length $record->{ "SEQ" }; $i++ )
+ {
+ $res = substr $record->{ "SEQ" }, $i, 1;
+
+ next if $res =~ /-|_|~|\./;
+
+ $freq_hash{ $i }{ $res }++;
+ }
+
+ $count++;
+ }
+ else
+ {
+ Maasha::Biopieces::put_record( $record, $out );
+ }
+}
+
+undef $record;
+
+if ( $type eq "protein" ) {
+ $bit_max = 4;
+} else {
+ $bit_max = 2;
+}
+
+for ( $i = 0; $i < keys %freq_hash; $i++ )
+{
+ $bit_height = Maasha::Seq::seqlogo_calc_bit_height( $freq_hash{ $i }, $count );
+
+ $bit_diff = $bit_max - $bit_height;
+
+ $record->{ "V" . ( $i ) } = sprintf( "%.2f", $bit_diff );
+}
+
+Maasha::Biopieces::put_record( $record, $out );
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+BEGIN
+{
+ $run_time_beg = Maasha::Biopieces::run_time();
+
+ Maasha::Biopieces::log_biopiece();
+}
+
+END
+{
+ Maasha::Biopieces::close_stream( $in );
+ Maasha::Biopieces::close_stream( $out );
+
+ $run_time_end = Maasha::Biopieces::run_time();
+
+ Maasha::Biopieces::run_time_print( $run_time_beg, $run_time_end, $options );
+}
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+__END__
+
-use Maasha::BioRun;
elsif ( $script eq "complexity_seq" ) { script_complexity_seq( $in, $out, $options ) }
elsif ( $script eq "oligo_freq" ) { script_oligo_freq( $in, $out, $options ) }
elsif ( $script eq "create_weight_matrix" ) { script_create_weight_matrix( $in, $out, $options ) }
- elsif ( $script eq "calc_bit_scores" ) { script_calc_bit_scores( $in, $out, $options ) }
elsif ( $script eq "calc_fixedstep" ) { script_calc_fixedstep( $in, $out, $options ) }
elsif ( $script eq "remove_indels" ) { script_remove_indels( $in, $out, $options ) }
elsif ( $script eq "transliterate_seq" ) { script_transliterate_seq( $in, $out, $options ) }
}
-sub script_calc_bit_scores
-{
- # Martin A. Hansen, March 2007.
-
- # Calculates the bit scores for each position from an alignmnet in the stream.
-
- my ( $in, # handle to in stream
- $out, # handle to out stream
- ) = @_;
-
- # Returns nothing.
-
- my ( $record, $type, $count, $i, $res, %freq_hash, $bit_max, $bit_height, $bit_diff );
-
- $count = 0;
-
- while ( $record = Maasha::Biopieces::get_record( $in ) )
- {
- if ( $record->{ "SEQ" } )
- {
- $type = Maasha::Seq::seq_guess_type( $record->{ "SEQ" } ) if not $type;
-
- for ( $i = 0; $i < length $record->{ "SEQ" }; $i++ )
- {
- $res = substr $record->{ "SEQ" }, $i, 1;
-
- next if $res =~ /-|_|~|\./;
-
- $freq_hash{ $i }{ $res }++;
- }
-
- $count++;
- }
- else
- {
- Maasha::Biopieces::put_record( $record, $out );
- }
- }
-
- undef $record;
-
- if ( $type eq "protein" ) {
- $bit_max = 4;
- } else {
- $bit_max = 2;
- }
-
- for ( $i = 0; $i < keys %freq_hash; $i++ )
- {
- $bit_height = Maasha::Seq::seqlogo_calc_bit_height( $freq_hash{ $i }, $count );
-
- $bit_diff = $bit_max - $bit_height;
-
- $record->{ "V" . ( $i ) } = sprintf( "%.2f", $bit_diff );
- }
-
- Maasha::Biopieces::put_record( $record, $out );
-}
-
-
sub script_calc_fixedstep
{
# Martin A. Hansen, September 2008.