T = globaldata->gTree;
tmap = globaldata->gTreemap;
- //weightedFile = globaldata->getTreeFile() + ".weighted";
- //openOutputFile(weightedFile, out);
- //column headers
- //out << "Group" << '\t' << "Score" << '\t' << "UserFreq" << '\t' << "UserCumul" << '\t' << "RandFreq" << '\t' << "RandCumul" << endl;
-
sumFile = globaldata->getTreeFile() + ".wsummary";
openOutputFile(sumFile, outSum);
- setGroups(); //sets the groups the user wants to analyze
+ util = new SharedUtil();
+ string s; //to make work with setgroups
+ util->setGroups(globaldata->Groups, tmap->namesOfGroups, s, numGroups, "weighted"); //sets the groups the user wants to analyze
+ util->getCombos(groupComb, globaldata->Groups, numComp);
+ globaldata->setGroups("");
+
convert(globaldata->getIters(), iters); //how many random trees to generate
weighted = new Weighted(tmap);
/***********************************************************/
int UnifracWeightedCommand::execute() {
try {
+ Progress* reading;
+ reading = new Progress("Comparing to random:", iters);
//get weighted for users tree
userData.resize(numComp,0); //data[0] = weightedscore AB, data[1] = weightedscore AC...
//create new tree with same num nodes and leaves as users
randT = new Tree();
- //get pscores for users trees
+ //get weighted scores for users trees
for (int i = 0; i < T.size(); i++) {
+ counter = 0;
rScores.resize(numComp); //data[0] = weightedscore AB, data[1] = weightedscore AC...
uScores.resize(numComp); //data[0] = weightedscore AB, data[1] = weightedscore AC...
- validScores.resize(numComp);
-
- cout << "Processing tree " << i+1 << endl;
+ //weightedFile = globaldata->getTreeFile() + toString(i+1) + ".weighted";
+ //weightedFileout = globaldata->getTreeFile() + "temp." + toString(i+1) + ".weighted";
+ output = new ThreeColumnFile2(globaldata->getTreeFile() + toString(i+1) + ".weighted");
+
userData = weighted->getValues(T[i]); //userData[0] = weightedscore
//save users score
//add users score to vector of user scores
uScores[s].push_back(userData[s]);
- //add users score to vector of valid scores
- validScores[s].push_back(userData[s]);
-
//save users tree score for summary file
utreeScores.push_back(userData[s]);
}
//save scores
rScores[count].push_back(randomData[0]);
- validScores[count][randomData[0]] = randomData[0];
count++;
}
}
+
+ //update progress bar
+ reading->update(j);
+
}
- removeValidScoresDuplicates();
+ //removeValidScoresDuplicates();
//find the signifigance of the score for summary file
for (int f = 0; f < numComp; f++) {
//sort random scores
}
//out << "Tree# " << i << endl;
- //printWeightedFile();
+ calculateFreqsCumuls();
+ printWeightedFile();
+
+ delete output;
//clear data
rScores.clear();
validScores.clear();
}
+ //finish progress bar
+ reading->finish();
+ delete reading;
+
printWSummaryFile();
//clear out users groups
exit(1);
}
}
-/***********************************************************
+/***********************************************************/
void UnifracWeightedCommand::printWeightedFile() {
try {
-
- //format output
- out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
+ vector<double> data;
+ vector<string> tags;
+ tags.push_back("Score"); tags.push_back("RandFreq"); tags.push_back("RandCumul");
- //for each group
- for (int e = 0; e < numComp; e++) {
- //print each line in that group
- for (i = 0; i < validScores[e].size(); i++) {
- out << setprecision(6) << groupComb[e] << '\t' << validScores[e][i] << '\t' << '\t' << uscoreFreq[e][it->first] << '\t' << uCumul[e][it->first] << '\t' << rscoreFreq[e][it->first] << '\t' << rCumul[e][it->first] << endl;
+ for(int a = 0; a < numComp; a++) {
+ output->initFile(groupComb[a], tags);
+ //print each line
+ for (it = validScores.begin(); it != validScores.end(); it++) {
+ data.push_back(it->first); data.push_back(rScoreFreq[a][it->first]); data.push_back(rCumul[a][it->first]);
+ output->output(data);
+ data.clear();
}
+ output->resetFile();
}
- out.close();
+ //out.close();
+ //inFile.close();
+ //remove(weightedFileout.c_str());
}
catch(exception& e) {
void UnifracWeightedCommand::printWSummaryFile() {
try {
//column headers
- outSum << "Tree#" << '\t' << "Groups" << '\t' << '\t' << "WScore" << '\t' << '\t' << "WSig" << endl;
- cout << "Tree#" << '\t' << "Groups" << '\t' << '\t' << "WScore" << '\t' << '\t' << "WSig" << endl;
+ outSum << "Tree#" << '\t' << "Groups" << '\t' << "WScore" << '\t' << "WSig" << endl;
+ cout << "Tree#" << '\t' << "Groups" << '\t' << "WScore" << '\t' << "WSig" << endl;
//format output
outSum.setf(ios::fixed, ios::floatfield); outSum.setf(ios::showpoint);
int count = 0;
for (int i = 0; i < T.size(); i++) {
for (int j = 0; j < numComp; j++) {
- outSum << setprecision(6) << i+1 << '\t' << '\t' << groupComb[j] << '\t' << utreeScores[count] << '\t' << WScoreSig[count] << endl;
- cout << setprecision(6) << i+1 << '\t' << '\t' << groupComb[j] << '\t' << utreeScores[count] << '\t' << WScoreSig[count] << endl;
+ if (WScoreSig[count] > (1/(float)iters)) {
+ outSum << setprecision(6) << i+1 << '\t' << groupComb[j] << '\t' << utreeScores[count] << '\t' << setprecision(globaldata->getIters().length()) << WScoreSig[count] << endl;
+ cout << setprecision(6) << i+1 << '\t' << groupComb[j] << '\t' << utreeScores[count] << '\t' << setprecision(globaldata->getIters().length()) << WScoreSig[count] << endl;
+ }else{
+ outSum << setprecision(6) << i+1 << '\t' << groupComb[j] << '\t' << utreeScores[count] << '\t' << setprecision(globaldata->getIters().length()) << "<" << (1/float(iters)) << endl;
+ cout << setprecision(6) << i+1 << '\t' << groupComb[j] << '\t' << utreeScores[count] << '\t' << setprecision(globaldata->getIters().length()) << "<" << (1/float(iters)) << endl;
+ }
count++;
}
}
}
/***********************************************************/
-void UnifracWeightedCommand::removeValidScoresDuplicates() {
+int UnifracWeightedCommand::findIndex(float score, int index) {
+ try{
+ for (int i = 0; i < rScores[index].size(); i++) {
+ if (rScores[index][i] >= score) { return i; }
+ }
+ return rScores[index].size();
+ }
+ catch(exception& e) {
+ cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function findIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ exit(1);
+ }
+ catch(...) {
+ cout << "An unknown error has occurred in the UnifracWeightedCommand class function findIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ exit(1);
+ }
+}
+
+/***********************************************************/
+
+void UnifracWeightedCommand::calculateFreqsCumuls() {
try {
- for (int e = 0; e < numComp; e++) {
- //sort valid scores
- sort(validScores[e].begin(), validScores[e].end());
-
- for (int i = 0; i< validScores[e].size()-1; i++) {
- if (validScores[e][i] == validScores[e][i+1]) { validScores[e].erase(validScores[e].begin()+i); }
+ //clear out old tree values
+ rScoreFreq.clear();
+ rScoreFreq.resize(numComp);
+ rCumul.clear();
+ rCumul.resize(numComp);
+ validScores.clear();
+
+ //calculate frequency
+ for (int f = 0; f < numComp; f++) {
+ for (int i = 0; i < rScores[f].size(); i++) { //looks like 0,0,1,1,1,2,4,7... you want to make a map that say rScoreFreq[0] = 2, rScoreFreq[1] = 3...
+ validScores[rScores[f][i]] = rScores[f][i];
+ it = rScoreFreq[f].find(rScores[f][i]);
+ if (it != rScoreFreq[f].end()) {
+ rScoreFreq[f][rScores[f][i]]++;
+ }else{
+ rScoreFreq[f][rScores[f][i]] = 1;
+ }
+ }
+ }
+
+ //calculate rcumul
+ for(int a = 0; a < numComp; a++) {
+ float rcumul = 1.0000;
+ //this loop fills the cumulative maps and put 0.0000 in the score freq map to make it easier to print.
+ for (it = validScores.begin(); it != validScores.end(); it++) {
+ //make rscoreFreq map and rCumul
+ it2 = rScoreFreq[a].find(it->first);
+ rCumul[a][it->first] = rcumul;
+ //get percentage of random trees with that info
+ if (it2 != rScoreFreq[a].end()) { rScoreFreq[a][it->first] /= iters; rcumul-= it2->second; }
+ else { rScoreFreq[a][it->first] = 0.0000; } //no random trees with that score
}
}
+
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function removeValidScoresDuplicates. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function calculateFreqsCums. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
exit(1);
}
catch(...) {
- cout << "An unknown error has occurred in the UnifracWeightedCommand class function removeValidScoresDuplicates. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ cout << "An unknown error has occurred in the UnifracWeightedCommand class function calculateFreqsCums. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
exit(1);
}
+
}
-/***********************************************************/
-int UnifracWeightedCommand::findIndex(float score, int index) {
- try{
- for (int i = 0; i < rScores[index].size(); i++) {
- if (rScores[index][i] >= score) { return i; }
+/*****************************************************************
+
+void UnifracWeightedCommand::initFile(string label){
+ try {
+ if(counter != 0){
+ openOutputFile(weightedFileout, out);
+ openInputFile(weightedFile, inFile);
+
+ string inputBuffer;
+ getline(inFile, inputBuffer);
+
+ out << inputBuffer << '\t' << label + "RandFreq" << '\t' << label + "RandCumul" << endl;
+ }else{
+ openOutputFile(weightedFileout, out);
+ out << label + "Score" << '\t' << label + "RandFreq" << '\t' << label + "RandCumul" << endl;
}
- return rScores[index].size();
+
+ out.setf(ios::fixed, ios::floatfield);
+ out.setf(ios::showpoint);
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function findIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function initFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
exit(1);
}
catch(...) {
- cout << "An unknown error has occurred in the UnifracWeightedCommand class function findIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ cout << "An unknown error has occurred in the UnifracWeightedCommand class function initFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
exit(1);
}
}
-/***********************************************************/
-void UnifracWeightedCommand::setGroups() {
+/***********************************************************************
+
+void UnifracWeightedCommand::output(vector<double> data){
try {
- //if the user has not entered specific groups to analyze then do them all
- if (globaldata->Groups.size() == 0) {
- numGroups = tmap->getNumGroups();
- for (int i=0; i < numGroups; i++) {
- globaldata->Groups.push_back(tmap->namesOfGroups[i]);
- }
- }else {
- if (globaldata->getGroups() != "all") {
- //check that groups are valid
- for (int i = 0; i < globaldata->Groups.size(); i++) {
- if (tmap->isValidGroup(globaldata->Groups[i]) != true) {
- cout << globaldata->Groups[i] << " is not a valid group, and will be disregarded." << endl;
- // erase the invalid group from globaldata->Groups
- globaldata->Groups.erase (globaldata->Groups.begin()+i);
- }
- }
-
- //if the user only entered invalid groups
- if (globaldata->Groups.size() == 0) {
- numGroups = tmap->getNumGroups();
- for (int i=0; i < numGroups; i++) {
- globaldata->Groups.push_back(tmap->namesOfGroups[i]);
- }
- cout << "When using the groups parameter you must have at least 2 valid groups. I will run the command using all the groups in your groupfile." << endl;
- }else if (globaldata->Groups.size() == 1) {
- cout << "When using the groups parameter you must have at least 2 valid groups. I will run the command using all the groups in your groupfile." << endl;
- numGroups = tmap->getNumGroups();
- globaldata->Groups.clear();
- for (int i=0; i < numGroups; i++) {
- globaldata->Groups.push_back(tmap->namesOfGroups[i]);
- }
- }else { numGroups = globaldata->Groups.size(); }
- }else { //users wants all groups
- numGroups = tmap->getNumGroups();
- globaldata->Groups.clear();
- globaldata->setGroups("");
- for (int i=0; i < numGroups; i++) {
- globaldata->Groups.push_back(tmap->namesOfGroups[i]);
- }
- }
+ if(counter != 0){
+ string inputBuffer;
+ getline(inFile, inputBuffer);
+
+ out << inputBuffer << '\t' << setprecision(6) << data[0] << setprecision(globaldata->getIters().length()) << '\t' << data[1] << '\t' << data[2] << endl;
+ }
+ else{
+ out << setprecision(6) << data[0] << setprecision(globaldata->getIters().length()) << '\t' << data[1] << '\t' << data[2] << endl;
+
}
- //calculate number of comparisons i.e. with groups A,B,C = AB, AC, BC = 3;
- numComp = 0;
- for (int i=0; i<numGroups; i++) {
- numComp += i;
- for (int l = i+1; l < numGroups; l++) {
- //set group comparison labels
- groupComb.push_back(globaldata->Groups[i]+globaldata->Groups[l]);
- }
+ }
+ catch(exception& e) {
+ cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function output. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ exit(1);
+ }
+ catch(...) {
+ cout << "An unknown error has occurred in the UnifracWeightedCommand class function output. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ exit(1);
+ }
+};
+
+/***********************************************************************
+
+void UnifracWeightedCommand::resetFile(){
+ try {
+ if(counter != 0){
+ out.close();
+ inFile.close();
}
+ else{
+ out.close();
+ }
+ counter = 1;
+
+ remove(weightedFile.c_str());
+ rename(weightedFileout.c_str(), weightedFile.c_str());
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ cout << "Standard Error: " << e.what() << " has occurred in the UnifracWeightedCommand class Function resetFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
exit(1);
}
catch(...) {
- cout << "An unknown error has occurred in the UnifracWeightedCommand class function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ cout << "An unknown error has occurred in the UnifracWeightedCommand class function resetFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
exit(1);
}
+
+
+
}
+*/
+
+
+
+