changed reading of name file to use buffered reads. note the splitAtWhiteSpace functi...

[mothur.git] / trimseqscommand.h

diff --git a/trimseqscommand.h b/trimseqscommand.h
index 780b4c7381cad9732d41b6fae8fc41736f6083e0..e280c8a1d4b50336f125dda5788d91d2af76c09a 100644 (file)
--- a/trimseqscommand.h
+++ b/trimseqscommand.h
@@ -51,6 +51,7 @@ private:
        bool cullLength(Sequence&);
        bool cullHomoP(Sequence&);
        bool cullAmbigs(Sequence&);
+    string reverseOligo(string);
 
        bool abort, createGroup;
        string fastaFile, oligoFile, qFileName, groupfile, nameFile, outputDir;
@@ -62,6 +63,7 @@ private:
        vector<string> revPrimer, outputNames;
        set<string> filesToRemove;
        map<string, int> barcodes;
+    map<string, int> rbarcodes;
        vector<string> groupVector;
        map<string, int> primers;
     vector<string>  linker;
@@ -100,6 +102,7 @@ struct trimData {
        double qRollAverage, qThreshold, qWindowAverage, qAverage;
     vector<string> revPrimer;
        map<string, int> barcodes;
+    map<string, int> rbarcodes;
        map<string, int> primers;
     vector<string>  linker;
     vector<string>  spacer;
@@ -111,7 +114,7 @@ struct trimData {
     
        trimData(){}
        trimData(string fn, string qn, string nf, string tn, string sn, string tqn, string sqn, string tnn, string snn, string gn, vector<vector<string> > ffn, vector<vector<string> > qfn, vector<vector<string> > nfn, unsigned long long lstart, unsigned long long lend, unsigned long long qstart, unsigned long long qend,  MothurOut* mout,
-                      int pd, int bd, int ld, int sd, int td, map<string, int> pri, map<string, int> bar, vector<string> revP, vector<string> li, vector<string> spa, 
+                      int pd, int bd, int ld, int sd, int td, map<string, int> pri, map<string, int> bar, map<string, int> rbar, vector<string> revP, vector<string> li, vector<string> spa, 
                       vector<string> priNameVector, vector<string> barNameVector, bool cGroup, bool aFiles, bool keepF, int keepfi, int removeL,
                       int WindowStep, int WindowSize, int WindowAverage, bool trim, double Threshold, double Average, double RollAverage,
                       int minL, int maxA, int maxH, int maxL, bool fli, map<string, string> nm) {
@@ -140,6 +143,7 @@ struct trimData {
         sdiffs = sd;
         tdiffs = td;
         barcodes = bar;
+        rbarcodes = rbar;
         primers = pri;      numFPrimers = primers.size();
         revPrimer = revP;   numRPrimers = revPrimer.size();
         linker = li;        numLinkers = linker.size();
@@ -236,7 +240,7 @@ static DWORD WINAPI MyTrimThreadFunction(LPVOID lpParam){
                }
                
                
-               TrimOligos trimOligos(pDataArray->pdiffs, pDataArray->bdiffs, pDataArray->ldiffs, pDataArray->sdiffs, pDataArray->primers, pDataArray->barcodes, pDataArray->revPrimer, pDataArray->linker, pDataArray->spacer);
+               TrimOligos trimOligos(pDataArray->pdiffs, pDataArray->bdiffs, pDataArray->ldiffs, pDataArray->sdiffs, pDataArray->primers, pDataArray->barcodes, pDataArray->rbarcodes, pDataArray->revPrimer, pDataArray->linker, pDataArray->spacer);
         
                pDataArray->count = pDataArray->lineEnd;
                for(int i = 0; i < pDataArray->lineEnd; i++){ //end is the number of sequences to process
@@ -276,7 +280,13 @@ static DWORD WINAPI MyTrimThreadFunction(LPVOID lpParam){
                                        if(success > pDataArray->bdiffs)                {       trashCode += 'b';       }
                                        else{ currentSeqsDiffs += success;  }
                                }
-                               
+                
+                               if(pDataArray->rbarcodes.size() != 0){
+                                       success = trimOligos.stripRBarcode(currSeq, currQual, barcodeIndex);
+                                       if(success > pDataArray->bdiffs)                {       trashCode += 'b';       }
+                                       else{ currentSeqsDiffs += success;  }
+                               }
+                
                 if(pDataArray->numSpacers != 0){
                                        success = trimOligos.stripSpacer(currSeq, currQual);
                                        if(success > pDataArray->sdiffs)                {       trashCode += 's';       }
@@ -403,11 +413,13 @@ static DWORD WINAPI MyTrimThreadFunction(LPVOID lpParam){
                                                        
                                                        outGroupsFile << currSeq.getName() << '\t' << thisGroup << endl;
                                                        
+                            int numRedundants = 0;
                                                        if (pDataArray->nameFile != "") {
                                                                map<string, string>::iterator itName = pDataArray->nameMap.find(currSeq.getName());
                                                                if (itName != pDataArray->nameMap.end()) { 
                                                                        vector<string> thisSeqsNames; 
                                                                        pDataArray->m->splitAtChar(itName->second, thisSeqsNames, ',');
+                                    numRedundants = thisSeqsNames.size()-1; //we already include ourselves below
                                                                        for (int k = 1; k < thisSeqsNames.size(); k++) { //start at 1 to skip self
                                                                                outGroupsFile << thisSeqsNames[k] << '\t' << thisGroup << endl;
                                                                        }
@@ -415,8 +427,8 @@ static DWORD WINAPI MyTrimThreadFunction(LPVOID lpParam){
                                                        }
                                                        
                                                        map<string, int>::iterator it = pDataArray->groupCounts.find(thisGroup);
-                                                       if (it == pDataArray->groupCounts.end()) {      pDataArray->groupCounts[thisGroup] = 1; }
-                                                       else { pDataArray->groupCounts[it->first]++; }
+                                                       if (it == pDataArray->groupCounts.end()) {      pDataArray->groupCounts[thisGroup] = 1 + numRedundants; }
+                                                       else { pDataArray->groupCounts[it->first] += (1 + numRedundants); }
                             
                                                }
                                        }