else {
//valid paramters for this command
- string AlignArray[] = {"fasta", "flip", "oligos", "maxambig", "maxhomop", "minlength", "maxlength", "qfile", "qthreshold", "qaverage", "allfiles", "qtrim"};
+ string AlignArray[] = {"fasta", "flip", "oligos", "maxambig", "maxhomop", "minlength", "maxlength", "qfile",
+ "qthreshold", "qaverage", "allfiles", "qtrim", "outputdir","inputdir"};
vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
map<string,string> parameters = parser.getParameters();
ValidParameters validParameter;
+ map<string,string>::iterator it;
//check to make sure all parameters are valid for command
- for (map<string,string>::iterator it = parameters.begin(); it != parameters.end(); it++) {
+ for (it = parameters.begin(); it != parameters.end(); it++) {
if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
+ //if the user changes the input directory command factory will send this info to us in the output parameter
+ string inputDir = validParameter.validFile(parameters, "inputdir", false);
+ if (inputDir == "not found"){ inputDir = ""; }
+ else {
+ string path;
+ it = parameters.find("fasta");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["fasta"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("oligos");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["oligos"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("qfile");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["qfile"] = inputDir + it->second; }
+ }
+ }
+
+
//check for required parameters
fastaFile = validParameter.validFile(parameters, "fasta", true);
if (fastaFile == "not found") { mothurOut("fasta is a required parameter for the screen.seqs command."); mothurOutEndLine(); abort = true; }
else if (fastaFile == "not open") { abort = true; }
-
+
+ //if the user changes the output directory command factory will send this info to us in the output parameter
+ outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
+ outputDir = "";
+ outputDir += hasPath(fastaFile); //if user entered a file with a path then preserve it
+ }
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
temp = validParameter.validFile(parameters, "qfile", true);
if (temp == "not found") { qFileName = ""; }
- else if(temp == "not open") { abort = 0; }
+ else if(temp == "not open") { abort = true; }
else { qFileName = temp; }
temp = validParameter.validFile(parameters, "qthreshold", false); if (temp == "not found") { temp = "0"; }
try{
if (abort == true) { return 0; }
-
+
+ numFPrimers = 0; //this needs to be initialized
+ numRPrimers = 0;
+
ifstream inFASTA;
openInputFile(fastaFile, inFASTA);
ofstream outFASTA;
- string trimSeqFile = getRootName(fastaFile) + "trim.fasta";
+ string trimSeqFile = outputDir + getRootName(getSimpleName(fastaFile)) + "trim.fasta";
openOutputFile(trimSeqFile, outFASTA);
ofstream outGroups;
vector<ofstream*> fastaFileNames;
if(oligoFile != ""){
- string groupFile = getRootName(fastaFile) + "groups";
+ string groupFile = outputDir + getRootName(getSimpleName(fastaFile)) + "groups";
openOutputFile(groupFile, outGroups);
getOligos(fastaFileNames);
}
ofstream scrapFASTA;
- string scrapSeqFile = getRootName(fastaFile) + "scrap.fasta";
+ string scrapSeqFile = outputDir + getRootName(getSimpleName(fastaFile)) + "scrap.fasta";
openOutputFile(scrapSeqFile, scrapFASTA);
ifstream qFile;
if(qFileName != "") { openInputFile(qFileName, qFile); }
bool success;
-
+
while(!inFASTA.eof()){
Sequence currSeq(inFASTA);
+
string origSeq = currSeq.getUnaligned();
if (origSeq != "") {
int group;
}
if(!success) { trashCode += 'q'; }
}
+
if(barcodes.size() != 0){
-
success = stripBarcode(currSeq, group);
if(!success){ trashCode += 'b'; }
}
+
if(numFPrimers != 0){
success = stripForward(currSeq);
if(!success){ trashCode += 'f'; }
}
+
if(numRPrimers != 0){
success = stripReverse(currSeq);
if(!success){ trashCode += 'r'; }
}
+
if(minLength > 0 || maxLength > 0){
success = cullLength(currSeq);
if(!success){ trashCode += 'l'; }
string seqName;
openInputFile(getRootName(fastaFile) + groupVector[i] + ".fasta", inFASTA);
ofstream outGroups;
- openOutputFile(getRootName(fastaFile) + groupVector[i] + ".groups", outGroups);
+ openOutputFile(outputDir + getRootName(getSimpleName(fastaFile)) + groupVector[i] + ".groups", outGroups);
while(!inFASTA.eof()){
if(inFASTA.get() == '>'){
outGroups.close();
inFASTA.close();
}
-
-
+
return 0;
}
catch(exception& e) {
forPrimer.push_back(oligo);
}
else if(type == "reverse"){
- revPrimer.push_back(oligo);
+ Sequence oligoRC("reverse", oligo);
+ oligoRC.reverseComplement();
+ revPrimer.push_back(oligoRC.getUnaligned());
}
else if(type == "barcode"){
inOligos >> group;
groupVector.push_back(group);
if(allFiles){
- outFASTAVec.push_back(new ofstream((getRootName(fastaFile) + group + ".fasta").c_str(), ios::ate));
+ outFASTAVec.push_back(new ofstream((outputDir + getRootName(getSimpleName(fastaFile)) + group + ".fasta").c_str(), ios::ate));
}
}
}
}
if(compareDNASeq(oligo, rawSequence.substr(rawSequence.length()-oligo.length(),oligo.length()))){
- seq.setUnaligned(rawSequence.substr(rawSequence.length()-oligo.length()));
+ seq.setUnaligned(rawSequence.substr(0,rawSequence.length()-oligo.length()));
success = 1;
break;
}
projects / mothur.git / blobdiff