#include "endiannessmacros.h"
//**********************************************************************************************************************
-vector<string> SffInfoCommand::getValidParameters(){
- try {
- string Array[] = {"sff","qfile","fasta","flow","trim","accnos","sfftxt","outputdir","inputdir", "outputdir"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+vector<string> SffInfoCommand::setParameters(){
+ try {
+ CommandParameter psff("sff", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(psff);
+ CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(paccnos);
+ CommandParameter psfftxt("sfftxt", "String", "", "", "", "", "",false,false); parameters.push_back(psfftxt);
+ CommandParameter pflow("flow", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pflow);
+ CommandParameter ptrim("trim", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(ptrim);
+ CommandParameter pfasta("fasta", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pfasta);
+ CommandParameter pqfile("name", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pqfile);
+ CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
+ CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+
+ vector<string> myArray;
+ for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
- m->errorOut(e, "SffInfoCommand", "getValidParameters");
+ m->errorOut(e, "SffInfoCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
-SffInfoCommand::SffInfoCommand(){
+string SffInfoCommand::getHelpString(){
try {
- abort = true; calledHelp = true;
- vector<string> tempOutNames;
- outputTypes["fasta"] = tempOutNames;
- outputTypes["flow"] = tempOutNames;
- outputTypes["sfftxt"] = tempOutNames;
- outputTypes["qual"] = tempOutNames;
+ string helpString = "";
+ helpString += "The sffinfo command reads a sff file and extracts the sequence data, or you can use it to parse a sfftxt file.\n";
+ helpString += "The sffinfo command parameters are sff, fasta, qfile, accnos, flow, sfftxt, and trim. sff is required. \n";
+ helpString += "The sff parameter allows you to enter the sff file you would like to extract data from. You may enter multiple files by separating them by -'s.\n";
+ helpString += "The fasta parameter allows you to indicate if you would like a fasta formatted file generated. Default=True. \n";
+ helpString += "The qfile parameter allows you to indicate if you would like a quality file generated. Default=True. \n";
+ helpString += "The flow parameter allows you to indicate if you would like a flowgram file generated. Default=False. \n";
+ helpString += "The sfftxt parameter allows you to indicate if you would like a sff.txt file generated. Default=False. \n";
+ helpString += "If you want to parse an existing sfftxt file into flow, fasta and quality file, enter the file name using the sfftxt parameter. \n";
+ helpString += "The trim parameter allows you to indicate if you would like a sequences and quality scores trimmed to the clipQualLeft and clipQualRight values. Default=True. \n";
+ helpString += "The accnos parameter allows you to provide a accnos file containing the names of the sequences you would like extracted. You may enter multiple files by separating them by -'s. \n";
+ helpString += "Example sffinfo(sff=mySffFile.sff, trim=F).\n";
+ helpString += "Note: No spaces between parameter labels (i.e. sff), '=' and parameters (i.e.yourSffFileName).\n\n";
+ return helpString;
}
catch(exception& e) {
- m->errorOut(e, "SffInfoCommand", "SffInfoCommand");
- exit(1);
- }
-}
-//**********************************************************************************************************************
-vector<string> SffInfoCommand::getRequiredParameters(){
- try {
- string Array[] = {"sff", "sfftxt", "or"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
- return myArray;
- }
- catch(exception& e) {
- m->errorOut(e, "SffInfoCommand", "getRequiredParameters");
+ m->errorOut(e, "SffInfoCommand", "getHelpString");
exit(1);
}
}
+
+
//**********************************************************************************************************************
-vector<string> SffInfoCommand::getRequiredFiles(){
+SffInfoCommand::SffInfoCommand(){
try {
- vector<string> myArray;
- return myArray;
+ abort = true; calledHelp = true;
+ setParameters();
+ vector<string> tempOutNames;
+ outputTypes["fasta"] = tempOutNames;
+ outputTypes["flow"] = tempOutNames;
+ outputTypes["sfftxt"] = tempOutNames;
+ outputTypes["qfile"] = tempOutNames;
}
catch(exception& e) {
- m->errorOut(e, "SffInfoCommand", "getRequiredFiles");
+ m->errorOut(e, "SffInfoCommand", "SffInfoCommand");
exit(1);
}
}
else {
//valid paramters for this command
- string Array[] = {"sff","qfile","fasta","flow","trim","accnos","sfftxt","outputdir","inputdir", "outputdir"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+ vector<string> myArray = setParameters();
OptionParser parser(option);
map<string, string> parameters = parser.getParameters();
outputTypes["fasta"] = tempOutNames;
outputTypes["flow"] = tempOutNames;
outputTypes["sfftxt"] = tempOutNames;
- outputTypes["qual"] = tempOutNames;
+ outputTypes["qfile"] = tempOutNames;
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; }
else if (sfftxtFilename == "not open") { sfftxtFilename = ""; }
}
- if ((sfftxtFilename == "") && (filenames.size() == 0)) { m->mothurOut("[ERROR]: you must provide a valid sff or sfftxt file."); m->mothurOutEndLine(); abort=true; }
+ if ((sfftxtFilename == "") && (filenames.size() == 0)) {
+ //if there is a current fasta file, use it
+ string filename = m->getSFFFile();
+ if (filename != "") { filenames.push_back(filename); m->mothurOut("Using " + filename + " as input file for the sff parameter."); m->mothurOutEndLine(); }
+ else { m->mothurOut("[ERROR]: you must provide a valid sff or sfftxt file."); m->mothurOutEndLine(); abort=true; }
+ }
}
}
catch(exception& e) {
exit(1);
}
}
-//**********************************************************************************************************************
-
-void SffInfoCommand::help(){
- try {
- m->mothurOut("The sffinfo command reads a sff file and extracts the sequence data, or you can use it to parse a sfftxt file..\n");
- m->mothurOut("The sffinfo command parameters are sff, fasta, qfile, accnos, flow, sfftxt, and trim. sff is required. \n");
- m->mothurOut("The sff parameter allows you to enter the sff file you would like to extract data from. You may enter multiple files by separating them by -'s.\n");
- m->mothurOut("The fasta parameter allows you to indicate if you would like a fasta formatted file generated. Default=True. \n");
- m->mothurOut("The qfile parameter allows you to indicate if you would like a quality file generated. Default=True. \n");
- m->mothurOut("The flow parameter allows you to indicate if you would like a flowgram file generated. Default=False. \n");
- m->mothurOut("The sfftxt parameter allows you to indicate if you would like a sff.txt file generated. Default=False. \n");
- m->mothurOut("If you want to parse an existing sfftxt file into flow, fasta and quality file, enter the file name using the sfftxt parameter. \n");
- m->mothurOut("The trim parameter allows you to indicate if you would like a sequences and quality scores trimmed to the clipQualLeft and clipQualRight values. Default=True. \n");
- m->mothurOut("The accnos parameter allows you to provide a accnos file containing the names of the sequences you would like extracted. You may enter multiple files by separating them by -'s. \n");
- m->mothurOut("Example sffinfo(sff=mySffFile.sff, trim=F).\n");
- m->mothurOut("Note: No spaces between parameter labels (i.e. sff), '=' and parameters (i.e.yourSffFileName).\n\n");
- }
- catch(exception& e) {
- m->errorOut(e, "SffInfoCommand", "help");
- exit(1);
- }
-}
-//**********************************************************************************************************************
-
-SffInfoCommand::~SffInfoCommand(){}
-
//**********************************************************************************************************************
int SffInfoCommand::execute(){
try {
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; }
+ //set fasta file as new current fastafile
+ string current = "";
+ itTypes = outputTypes.find("fasta");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
+ }
+
+ itTypes = outputTypes.find("qfile");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); }
+ }
+
//report output filenames
m->mothurOutEndLine();
m->mothurOut("Output File Names: "); m->mothurOutEndLine();
if (sfftxt) { m->openOutputFile(sfftxtFileName, outSfftxt); outSfftxt.setf(ios::fixed, ios::floatfield); outSfftxt.setf(ios::showpoint); outputNames.push_back(sfftxtFileName); outputTypes["sfftxt"].push_back(sfftxtFileName); }
if (fasta) { m->openOutputFile(outFastaFileName, outFasta); outputNames.push_back(outFastaFileName); outputTypes["fasta"].push_back(outFastaFileName); }
- if (qual) { m->openOutputFile(outQualFileName, outQual); outputNames.push_back(outQualFileName); outputTypes["qual"].push_back(outQualFileName); }
+ if (qual) { m->openOutputFile(outQualFileName, outQual); outputNames.push_back(outQualFileName); outputTypes["qfile"].push_back(outQualFileName); }
if (flow) { m->openOutputFile(outFlowFileName, outFlow); outputNames.push_back(outFlowFileName); outFlow.setf(ios::fixed, ios::floatfield); outFlow.setf(ios::showpoint); outputTypes["flow"].push_back(outFlowFileName); }
ifstream in;
CommonHeader header;
readCommonHeader(in, header);
-
+
int count = 0;
+ mycount = 0;
//check magic number and version
if (header.magicNumber != 779314790) { m->mothurOut("Magic Number is not correct, not a valid .sff file"); m->mothurOutEndLine(); return count; }
}
count++;
+ mycount++;
//report progress
if((count+1) % 10000 == 0){ m->mothurOut(toString(count+1)); m->mothurOutEndLine(); }
int SffInfoCommand::decodeName(string& timestamp, string& region, string& xy, string name) {
try {
- string time = name.substr(0, 6);
- unsigned int timeNum = m->fromBase36(time);
-
- int q1 = timeNum / 60;
- int sec = timeNum - 60 * q1;
- int q2 = q1 / 60;
- int minute = q1 - 60 * q2;
- int q3 = q2 / 24;
- int hr = q2 - 24 * q3;
- int q4 = q3 / 32;
- int day = q3 - 32 * q4;
- int q5 = q4 / 13;
- int mon = q4 - 13 * q5;
- int year = 2000 + q5;
-
- timestamp = toString(year) + "_" + toString(mon) + "_" + toString(day) + "_" + toString(hr) + "_" + toString(minute) + "_" + toString(sec);
-
- region = name.substr(7, 2);
-
- string xyNum = name.substr(9);
- unsigned int myXy = m->fromBase36(xyNum);
- int x = myXy >> 12;
- int y = myXy & 4095;
-
- xy = toString(x) + "_" + toString(y);
+ if (name.length() >= 6) {
+ string time = name.substr(0, 6);
+ unsigned int timeNum = m->fromBase36(time);
+ int q1 = timeNum / 60;
+ int sec = timeNum - 60 * q1;
+ int q2 = q1 / 60;
+ int minute = q1 - 60 * q2;
+ int q3 = q2 / 24;
+ int hr = q2 - 24 * q3;
+ int q4 = q3 / 32;
+ int day = q3 - 32 * q4;
+ int q5 = q4 / 13;
+ int mon = q4 - 13 * q5;
+ int year = 2000 + q5;
+
+ timestamp = toString(year) + "_" + toString(mon) + "_" + toString(day) + "_" + toString(hr) + "_" + toString(minute) + "_" + toString(sec);
+ }
+
+ if (name.length() >= 9) {
+ region = name.substr(7, 2);
+
+ string xyNum = name.substr(9);
+ unsigned int myXy = m->fromBase36(xyNum);
+ int x = myXy >> 12;
+ int y = myXy & 4095;
+
+ xy = toString(x) + "_" + toString(y);
+ }
+
return 0;
}
catch(exception& e) {
if (trim) {
if(header.clipQualRight < header.clipQualLeft){
+ out << ">" << header.name << " xy=" << header.xy << endl;
out << "0\t0\t0\t0";
}
else if((header.clipQualRight != 0) && ((header.clipQualRight-header.clipQualLeft) >= 0)){
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