#include "sffinfocommand.h"
#include "endiannessmacros.h"
+//**********************************************************************************************************************
+vector<string> SffInfoCommand::getValidParameters(){
+ try {
+ string Array[] = {"sff","qfile","fasta","flow","trim","accnos","sfftxt","outputdir","inputdir", "outputdir"};
+ vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SffInfoCommand", "getValidParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+SffInfoCommand::SffInfoCommand(){
+ try {
+ abort = true;
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["fasta"] = tempOutNames;
+ outputTypes["flow"] = tempOutNames;
+ outputTypes["sfftxt"] = tempOutNames;
+ outputTypes["qual"] = tempOutNames;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SffInfoCommand", "SffInfoCommand");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+vector<string> SffInfoCommand::getRequiredParameters(){
+ try {
+ string Array[] = {"sff"};
+ vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SffInfoCommand", "getRequiredParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+vector<string> SffInfoCommand::getRequiredFiles(){
+ try {
+ vector<string> myArray;
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SffInfoCommand", "getRequiredFiles");
+ exit(1);
+ }
+}
//**********************************************************************************************************************
SffInfoCommand::SffInfoCommand(string option) {
if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["fasta"] = tempOutNames;
+ outputTypes["flow"] = tempOutNames;
+ outputTypes["sfftxt"] = tempOutNames;
+ outputTypes["qual"] = tempOutNames;
+
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; }
sffFilename = validParameter.validFile(parameters, "sff", false);
if (sffFilename == "not found") { m->mothurOut("sff is a required parameter for the sffinfo command."); m->mothurOutEndLine(); abort = true; }
else {
- splitAtDash(sffFilename, filenames);
+ m->splitAtDash(sffFilename, filenames);
//go through files and make sure they are good, if not, then disregard them
for (int i = 0; i < filenames.size(); i++) {
if (inputDir != "") {
- string path = hasPath(filenames[i]);
+ string path = m->hasPath(filenames[i]);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { filenames[i] = inputDir + filenames[i]; }
}
ifstream in;
- int ableToOpen = openInputFile(filenames[i], in, "noerror");
+ int ableToOpen = m->openInputFile(filenames[i], in, "noerror");
//if you can't open it, try default location
if (ableToOpen == 1) {
if (m->getDefaultPath() != "") { //default path is set
- string tryPath = m->getDefaultPath() + getSimpleName(filenames[i]);
+ string tryPath = m->getDefaultPath() + m->getSimpleName(filenames[i]);
m->mothurOut("Unable to open " + filenames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
- ableToOpen = openInputFile(tryPath, in, "noerror");
+ ifstream in2;
+ ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+ in2.close();
+ filenames[i] = tryPath;
+ }
+ }
+
+ //if you can't open it, try default location
+ if (ableToOpen == 1) {
+ if (m->getOutputDir() != "") { //default path is set
+ string tryPath = m->getOutputDir() + m->getSimpleName(filenames[i]);
+ m->mothurOut("Unable to open " + filenames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+ ifstream in2;
+ ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+ in2.close();
filenames[i] = tryPath;
}
}
+
in.close();
if (ableToOpen == 1) {
if (accnosName == "not found") { accnosName = ""; }
else {
hasAccnos = true;
- splitAtDash(accnosName, accnosFileNames);
+ m->splitAtDash(accnosName, accnosFileNames);
//go through files and make sure they are good, if not, then disregard them
for (int i = 0; i < accnosFileNames.size(); i++) {
if (inputDir != "") {
- string path = hasPath(accnosFileNames[i]);
+ string path = m->hasPath(accnosFileNames[i]);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { accnosFileNames[i] = inputDir + accnosFileNames[i]; }
}
ifstream in;
- int ableToOpen = openInputFile(accnosFileNames[i], in, "noerror");
+ int ableToOpen = m->openInputFile(accnosFileNames[i], in, "noerror");
//if you can't open it, try default location
if (ableToOpen == 1) {
if (m->getDefaultPath() != "") { //default path is set
- string tryPath = m->getDefaultPath() + getSimpleName(accnosFileNames[i]);
+ string tryPath = m->getDefaultPath() + m->getSimpleName(accnosFileNames[i]);
m->mothurOut("Unable to open " + accnosFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
- ableToOpen = openInputFile(tryPath, in, "noerror");
+ ifstream in2;
+ ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+ in2.close();
+ accnosFileNames[i] = tryPath;
+ }
+ }
+ //if you can't open it, try default location
+ if (ableToOpen == 1) {
+ if (m->getOutputDir() != "") { //default path is set
+ string tryPath = m->getOutputDir() + m->getSimpleName(accnosFileNames[i]);
+ m->mothurOut("Unable to open " + accnosFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+ ifstream in2;
+ ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+ in2.close();
accnosFileNames[i] = tryPath;
}
}
}
string temp = validParameter.validFile(parameters, "qfile", false); if (temp == "not found"){ temp = "T"; }
- qual = isTrue(temp);
+ qual = m->isTrue(temp);
temp = validParameter.validFile(parameters, "fasta", false); if (temp == "not found"){ temp = "T"; }
- fasta = isTrue(temp);
+ fasta = m->isTrue(temp);
temp = validParameter.validFile(parameters, "flow", false); if (temp == "not found"){ temp = "F"; }
- flow = isTrue(temp);
+ flow = m->isTrue(temp);
temp = validParameter.validFile(parameters, "trim", false); if (temp == "not found"){ temp = "T"; }
- trim = isTrue(temp);
+ trim = m->isTrue(temp);
temp = validParameter.validFile(parameters, "sfftxt", false); if (temp == "not found"){ temp = "F"; }
- sfftxt = isTrue(temp);
+ sfftxt = m->isTrue(temp);
}
}
catch(exception& e) {
int SffInfoCommand::extractSffInfo(string input, string accnos){
try {
- if (outputDir == "") { outputDir += hasPath(input); }
+ if (outputDir == "") { outputDir += m->hasPath(input); }
if (accnos != "") { readAccnosFile(accnos); }
else { seqNames.clear(); }
ofstream outSfftxt, outFasta, outQual, outFlow;
string outFastaFileName, outQualFileName;
- string sfftxtFileName = outputDir + getRootName(getSimpleName(input)) + "sff.txt";
- string outFlowFileName = outputDir + getRootName(getSimpleName(input)) + "flow";
+ string sfftxtFileName = outputDir + m->getRootName(m->getSimpleName(input)) + "sff.txt";
+ string outFlowFileName = outputDir + m->getRootName(m->getSimpleName(input)) + "flow";
if (trim) {
- outFastaFileName = outputDir + getRootName(getSimpleName(input)) + "fasta";
- outQualFileName = outputDir + getRootName(getSimpleName(input)) + "qual";
+ outFastaFileName = outputDir + m->getRootName(m->getSimpleName(input)) + "fasta";
+ outQualFileName = outputDir + m->getRootName(m->getSimpleName(input)) + "qual";
}else{
- outFastaFileName = outputDir + getRootName(getSimpleName(input)) + "raw.fasta";
- outQualFileName = outputDir + getRootName(getSimpleName(input)) + "raw.qual";
+ outFastaFileName = outputDir + m->getRootName(m->getSimpleName(input)) + "raw.fasta";
+ outQualFileName = outputDir + m->getRootName(m->getSimpleName(input)) + "raw.qual";
}
- if (sfftxt) { openOutputFile(sfftxtFileName, outSfftxt); outSfftxt.setf(ios::fixed, ios::floatfield); outSfftxt.setf(ios::showpoint); outputNames.push_back(sfftxtFileName); }
- if (fasta) { openOutputFile(outFastaFileName, outFasta); outputNames.push_back(outFastaFileName); }
- if (qual) { openOutputFile(outQualFileName, outQual); outputNames.push_back(outQualFileName); }
- if (flow) { openOutputFile(outFlowFileName, outFlow); outputNames.push_back(outFlowFileName); }
+ if (sfftxt) { m->openOutputFile(sfftxtFileName, outSfftxt); outSfftxt.setf(ios::fixed, ios::floatfield); outSfftxt.setf(ios::showpoint); outputNames.push_back(sfftxtFileName); outputTypes["sfftxt"].push_back(sfftxtFileName); }
+ if (fasta) { m->openOutputFile(outFastaFileName, outFasta); outputNames.push_back(outFastaFileName); outputTypes["fasta"].push_back(outFastaFileName); }
+ if (qual) { m->openOutputFile(outQualFileName, outQual); outputNames.push_back(outQualFileName); outputTypes["qual"].push_back(outQualFileName); }
+ if (flow) { m->openOutputFile(outFlowFileName, outFlow); outputNames.push_back(outFlowFileName); outputTypes["flow"].push_back(outFlowFileName); }
ifstream in;
in.open(input.c_str(), ios::binary);
char buffer4 [2];
in.read(buffer4, 2);
header.clipQualLeft = be_int2(*(unsigned short *)(&buffer4));
+ header.clipQualLeft = 5;
//read clip qual right
char buffer5 [2];
in.read(buffer5, 2);
header.clipQualRight = be_int2(*(unsigned short *)(&buffer5));
- if(header.clipQualRight == 0){ header.clipQualRight = numBases; }
//read clipAdapterLeft
char buffer6 [2];
if (header.name.length() > header.nameLength) { header.name = header.name.substr(0, header.nameLength); }
delete[] tempBuffer;
+ //extract info from name
+ decodeName(header.timestamp, header.region, header.xy, header.name);
+
/* Pad to 8 chars */
unsigned long int spotInFile = in.tellg();
unsigned long int spot = (spotInFile + 7)& ~7;
}
}
//**********************************************************************************************************************
+int SffInfoCommand::decodeName(string& timestamp, string& region, string& xy, string name) {
+ try {
+
+ string time = name.substr(0, 6);
+ unsigned int timeNum = m->fromBase36(time);
+
+ int q1 = timeNum / 60;
+ int sec = timeNum - 60 * q1;
+ int q2 = q1 / 60;
+ int minute = q1 - 60 * q2;
+ int q3 = q2 / 24;
+ int hr = q2 - 24 * q3;
+ int q4 = q3 / 32;
+ int day = q3 - 32 * q4;
+ int q5 = q4 / 13;
+ int mon = q4 - 13 * q5;
+ int year = 2000 + q5;
+
+ timestamp = toString(year) + "_" + toString(mon) + "_" + toString(day) + "_" + toString(hr) + "_" + toString(minute) + "_" + toString(sec);
+
+ region = name.substr(7, 2);
+
+ string xyNum = name.substr(9);
+ unsigned int myXy = m->fromBase36(xyNum);
+ int x = myXy >> 12;
+ int y = myXy & 4095;
+
+ xy = toString(x) + "_" + toString(y);
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SffInfoCommand", "decodeName");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
int SffInfoCommand::printCommonHeader(ofstream& out, CommonHeader& header) {
try {
try {
out << ">" << header.name << endl;
- out << "Run Prefix: " << endl;
- out << "Region #: " << endl;
- out << "XY Location: " << endl << endl;
+ out << "Run Prefix: " << header.timestamp << endl;
+ out << "Region #: " << header.region << endl;
+ out << "XY Location: " << header.xy << endl << endl;
out << "Run Name: " << endl;
out << "Analysis Name: " << endl;
for (int i = 0; i < read.flowIndex.size(); i++) { sum += read.flowIndex[i]; out << sum << '\t'; }
//make the bases you want to clip lowercase and the bases you want to keep upper case
+ if(header.clipQualRight == 0){ header.clipQualRight = read.bases.length(); }
for (int i = 0; i < (header.clipQualLeft-1); i++) { read.bases[i] = tolower(read.bases[i]); }
for (int i = (header.clipQualLeft-1); i < (header.clipQualRight-1); i++) { read.bases[i] = toupper(read.bases[i]); }
for (int i = (header.clipQualRight-1); i < read.bases.length(); i++) { read.bases[i] = tolower(read.bases[i]); }
string seq = read.bases;
if (trim) {
- seq = seq.substr((header.clipQualLeft-1), (header.clipQualRight-header.clipQualLeft+1));
+ if(header.clipQualRight < header.clipQualLeft){
+ seq = "NNNN";
+ }
+ else if((header.clipQualRight != 0) && ((header.clipQualRight-header.clipQualLeft) >= 0)){
+ seq = seq.substr((header.clipQualLeft-1), (header.clipQualRight-header.clipQualLeft));
+ }
+ else {
+ seq = seq.substr(header.clipQualLeft-1);
+ }
}else{
//if you wanted the sfftxt then you already converted the bases to the right case
if (!sfftxt) {
//make the bases you want to clip lowercase and the bases you want to keep upper case
+ if(header.clipQualRight == 0){ header.clipQualRight = seq.length(); }
for (int i = 0; i < (header.clipQualLeft-1); i++) { seq[i] = tolower(seq[i]); }
for (int i = (header.clipQualLeft-1); i < (header.clipQualRight-1); i++) { seq[i] = toupper(seq[i]); }
for (int i = (header.clipQualRight-1); i < seq.length(); i++) { seq[i] = tolower(seq[i]); }
}
}
- out << ">" << header.name << endl;
+ out << ">" << header.name << " xy=" << header.xy << endl;
out << seq << endl;
return 0;
try {
if (trim) {
- out << ">" << header.name << " length=" << (header.clipQualRight-header.clipQualLeft+1) << endl;
- for (int i = (header.clipQualLeft-1); i < (header.clipQualRight-1); i++) { out << read.qualScores[i] << '\t'; }
+ if(header.clipQualRight < header.clipQualLeft){
+ out << "0\t0\t0\t0";
+ }
+ else if((header.clipQualRight != 0) && ((header.clipQualRight-header.clipQualLeft) >= 0)){
+ out << ">" << header.name << " xy=" << header.xy << " length=" << (header.clipQualRight-header.clipQualLeft) << endl;
+ for (int i = (header.clipQualLeft-1); i < (header.clipQualRight-1); i++) { out << read.qualScores[i] << '\t'; }
+ }
+ else{
+ out << ">" << header.name << " xy=" << header.xy << " length=" << (header.clipQualRight-header.clipQualLeft) << endl;
+ for (int i = (header.clipQualLeft-1); i < read.qualScores.size(); i++) { out << read.qualScores[i] << '\t'; }
+ }
}else{
- out << ">" << header.name << " length=" << read.qualScores.size() << endl;
+ out << ">" << header.name << " xy=" << header.xy << " length=" << read.qualScores.size() << endl;
for (int i = 0; i < read.qualScores.size(); i++) { out << read.qualScores[i] << '\t'; }
}
int SffInfoCommand::printFlowSeqData(ofstream& out, seqRead& read, Header& header) {
try {
- out << ">" << header.name << endl;
+ out << ">" << header.name << " xy=" << header.xy << endl;
for (int i = 0; i < read.flowgram.size(); i++) { out << setprecision(2) << (read.flowgram[i]/(float)100) << '\t'; }
out << endl;
seqNames.clear();
ifstream in;
- openInputFile(filename, in);
+ m->openInputFile(filename, in);
string name;
while(!in.eof()){
- in >> name; gobble(in);
+ in >> name; m->gobble(in);
seqNames.insert(name);
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