#include "sequence.hpp"
/***********************************************************************/
-
Sequence::Sequence(){
m = MothurOut::getInstance();
initialize();
}
-
/***********************************************************************/
-
Sequence::Sequence(string newName, string sequence) {
try {
m = MothurOut::getInstance();
exit(1);
}
}
+/***********************************************************************/
+Sequence::Sequence(string newName, string sequence, string justUnAligned) {
+ try {
+ m = MothurOut::getInstance();
+ initialize();
+ name = newName;
+
+ //setUnaligned removes any gap characters for us
+ setUnaligned(sequence);
+ }
+ catch(exception& e) {
+ m->errorOut(e, "Sequence", "Sequence");
+ exit(1);
+ }
+}
+
//********************************************************************************************************************
//this function will jump over commented out sequences, but if the last sequence in a file is commented out it makes a blank seq
Sequence::Sequence(istringstream& fastaString){
initialize();
fastaString >> name;
- name = name.substr(1);
- string sequence;
-
- //read comments
- while ((name[0] == '#') && fastaString) {
- while (!fastaString.eof()) { char c = fastaString.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
- sequence = getCommentString(fastaString);
-
- if (fastaString) {
- fastaString >> name;
- name = name.substr(1);
- }else {
- name = "";
- break;
+
+ if (name.length() != 0) {
+
+ name = name.substr(1);
+ string sequence;
+
+ //read comments
+ while ((name[0] == '#') && fastaString) {
+ while (!fastaString.eof()) { char c = fastaString.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+ sequence = getCommentString(fastaString);
+
+ if (fastaString) {
+ fastaString >> name;
+ name = name.substr(1);
+ }else {
+ name = "";
+ break;
+ }
}
- }
+
+ while (!fastaString.eof()) { char c = fastaString.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+
+ sequence = getSequenceString(fastaString);
+ setAligned(sequence);
+ //setUnaligned removes any gap characters for us
+ setUnaligned(sequence);
+ }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaString.tellg()) + ". Blank name."); m->mothurOutEndLine(); }
- while (!fastaString.eof()) { char c = fastaString.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+ }
+ catch(exception& e) {
+ m->errorOut(e, "Sequence", "Sequence");
+ exit(1);
+ }
+}
+//********************************************************************************************************************
+//this function will jump over commented out sequences, but if the last sequence in a file is commented out it makes a blank seq
+Sequence::Sequence(istringstream& fastaString, string JustUnaligned){
+ try {
+ m = MothurOut::getInstance();
+
+ initialize();
+ fastaString >> name;
+
+ if (name.length() != 0) {
+
+ name = name.substr(1);
+ string sequence;
+
+ //read comments
+ while ((name[0] == '#') && fastaString) {
+ while (!fastaString.eof()) { char c = fastaString.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+ sequence = getCommentString(fastaString);
+
+ if (fastaString) {
+ fastaString >> name;
+ name = name.substr(1);
+ }else {
+ name = "";
+ break;
+ }
+ }
+
+ while (!fastaString.eof()) { char c = fastaString.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+
+ sequence = getSequenceString(fastaString);
+
+ //setUnaligned removes any gap characters for us
+ setUnaligned(sequence);
+ }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaString.tellg()) + ". Blank name."); m->mothurOutEndLine(); }
- sequence = getSequenceString(fastaString);
- setAligned(sequence);
- //setUnaligned removes any gap characters for us
- setUnaligned(sequence);
}
catch(exception& e) {
m->errorOut(e, "Sequence", "Sequence");
}
}
+
//********************************************************************************************************************
//this function will jump over commented out sequences, but if the last sequence in a file is commented out it makes a blank seq
Sequence::Sequence(ifstream& fastaFile){
m = MothurOut::getInstance();
initialize();
fastaFile >> name;
- name = name.substr(1);
- string sequence;
- //read comments
- while ((name[0] == '#') && fastaFile) {
- while (!fastaFile.eof()) { char c = fastaFile.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
- sequence = getCommentString(fastaFile);
+ if (name.length() != 0) {
+
+ name = name.substr(1);
- if (fastaFile) {
- fastaFile >> name;
- name = name.substr(1);
- }else {
- name = "";
- break;
- }
- }
+ string sequence;
- //read real sequence
- while (!fastaFile.eof()) { char c = fastaFile.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+ //read comments
+ while ((name[0] == '#') && fastaFile) {
+ while (!fastaFile.eof()) { char c = fastaFile.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+ sequence = getCommentString(fastaFile);
+
+ if (fastaFile) {
+ fastaFile >> name;
+ name = name.substr(1);
+ }else {
+ name = "";
+ break;
+ }
+ }
+
+ //read real sequence
+ while (!fastaFile.eof()) { char c = fastaFile.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+
+ sequence = getSequenceString(fastaFile);
+
+ setAligned(sequence);
+ //setUnaligned removes any gap characters for us
+ setUnaligned(sequence);
+ }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); }
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "Sequence", "Sequence");
+ exit(1);
+ }
+}
+//********************************************************************************************************************
+//this function will jump over commented out sequences, but if the last sequence in a file is commented out it makes a blank seq
+Sequence::Sequence(ifstream& fastaFile, string JustUnaligned){
+ try {
+ m = MothurOut::getInstance();
+ initialize();
+ fastaFile >> name;
- sequence = getSequenceString(fastaFile);
+ if (name.length() != 0) {
+ name = name.substr(1);
+ string sequence;
+
+ //read comments
+ while ((name[0] == '#') && fastaFile) {
+ while (!fastaFile.eof()) { char c = fastaFile.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+ sequence = getCommentString(fastaFile);
+
+ if (fastaFile) {
+ fastaFile >> name;
+ name = name.substr(1);
+ }else {
+ name = "";
+ break;
+ }
+ }
+
+ //read real sequence
+ while (!fastaFile.eof()) { char c = fastaFile.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
+
+ sequence = getSequenceString(fastaFile);
+
+ //setUnaligned removes any gap characters for us
+ setUnaligned(sequence);
+ }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); }
- setAligned(sequence);
- //setUnaligned removes any gap characters for us
- setUnaligned(sequence);
}
catch(exception& e) {
m->errorOut(e, "Sequence", "Sequence");
exit(1);
}
}
+
//********************************************************************************************************************
string Sequence::getSequenceString(ifstream& fastaFile) {
try {
while(fastaFile){
letter=fastaFile.get();
if((letter == '\r') || (letter == '\n')){
- gobble(fastaFile); //in case its a \r\n situation
+ m->gobble(fastaFile); //in case its a \r\n situation
break;
}
}
while(fastaFile){
letter=fastaFile.get();
if((letter == '\r') || (letter == '\n')){
- gobble(fastaFile); //in case its a \r\n situation
+ m->gobble(fastaFile); //in case its a \r\n situation
break;
}
}
//********************************************************************************************************************
string Sequence::getAligned(){
- return aligned;
+ if(isAligned == 0) { return unaligned; }
+ else { return aligned; }
}
//********************************************************************************************************************
//********************************************************************************************************************
+void Sequence::removeAmbigBases(){
+
+ for(int j=0;j<alignmentLength;j++){
+ if(aligned[j] != 'A' && aligned[j] != 'T' && aligned[j] != 'G' && aligned[j] != 'C'){
+ aligned[j] = '-';
+ }
+ }
+ setUnaligned(aligned);
+}
+
+//********************************************************************************************************************
+
int Sequence::getLongHomoPolymer(){
if(longHomoPolymer == -1){
longHomoPolymer = 1;
aligned = temp;
}
-#ifdef USE_MPI
+
//********************************************************************************************************************
-int Sequence::MPISend(int receiver) {
- try {
- //send name - string
- int length = name.length();
- char buf[name.length()];
- strcpy(buf, name.c_str());
-
- MPI_Send(&length, 1, MPI_INT, receiver, 2001, MPI_COMM_WORLD);
- MPI_Send(&buf, length, MPI_CHAR, receiver, 2001, MPI_COMM_WORLD);
-
- //send aligned - string
- length = aligned.length();
- char buf2[aligned.length()];
- strcpy(buf2, aligned.c_str());
+void Sequence::trim(int length){
- MPI_Send(&length, 1, MPI_INT, receiver, 2001, MPI_COMM_WORLD);
-
- MPI_Send(&buf2, length, MPI_CHAR, receiver, 2001, MPI_COMM_WORLD);
-
- return 0;
-
- }
- catch(exception& e) {
- m->errorOut(e, "Sequence", "MPISend");
- exit(1);
+ if(numBases > length){
+ unaligned = unaligned.substr(0,length);
+ numBases = length;
}
-}
-/**************************************************************************************************/
-int Sequence::MPIRecv(int sender) {
- try {
- MPI_Status status;
- //receive name - string
- int length;
- MPI_Recv(&length, 1, MPI_INT, sender, 2001, MPI_COMM_WORLD, &status);
-
- char buf[length];
- MPI_Recv(&buf, length, MPI_CHAR, sender, 2001, MPI_COMM_WORLD, &status);
- name = buf;
-
- //receive aligned - string
- MPI_Recv(&length, 1, MPI_INT, sender, 2001, MPI_COMM_WORLD, &status);
-
- char buf2[length];
- MPI_Recv(&buf2, length, MPI_CHAR, sender, 2001, MPI_COMM_WORLD, &status);
- aligned = buf2;
-
- setAligned(aligned);
-
- return 0;
-
- }
- catch(exception& e) {
- m->errorOut(e, "Sequence", "MPIRecv");
- exit(1);
- }
}
-#endif
-/**************************************************************************************************/
+
+///**************************************************************************************************/