//**********************************************************************************************************************
SeqErrorCommand::SeqErrorCommand(){
try {
- abort = true;
- //initialize outputTypes
+ abort = true; calledHelp = true;
vector<string> tempOutNames;
outputTypes["error.summary"] = tempOutNames;
outputTypes["error.seq"] = tempOutNames;
SeqErrorCommand::SeqErrorCommand(string option) {
try {
- abort = false;
+ abort = false; calledHelp = false;
//allow user to run help
- if(option == "help") { help(); abort = true; }
+ if(option == "help") { help(); abort = true; calledHelp = true; }
else {
string temp;
convert(temp, ignoreChimeras);
substitutionMatrix.resize(6);
- for(int i=0;i<6;i++){ substitutionMatrix[i].assign(6,0); }
+ for(int i=0;i<6;i++){ substitutionMatrix[i].resize(6,0); }
}
}
catch(exception& e) {
//***************************************************************************************************************
-SeqErrorCommand::~SeqErrorCommand(){
-
-}
+SeqErrorCommand::~SeqErrorCommand(){ /* void */ }
//***************************************************************************************************************
int SeqErrorCommand::execute(){
try{
- if (abort == true) { return 0; }
+ if (abort == true) { if (calledHelp) { return 0; } return 2; }
+ maxLength = 2000;
+
string errorSummaryFileName = queryFileName.substr(0,queryFileName.find_last_of('.')) + ".error.summary";
m->openOutputFile(errorSummaryFileName, errorSummaryFile);
outputNames.push_back(errorSummaryFileName); outputTypes["error.summary"].push_back(errorSummaryFileName);
m->openInputFile(qualFileName, qualFile);
report = ReportFile(reportFile, reportFileName);
- qualForwardMap.resize(1000);
- qualReverseMap.resize(1000);
- for(int i=0;i<1000;i++){
- qualForwardMap[i].assign(100,0);
- qualReverseMap[i].assign(100,0);
+ qualForwardMap.resize(maxLength);
+ qualReverseMap.resize(maxLength);
+ for(int i=0;i<maxLength;i++){
+ qualForwardMap[i].assign(maxLength,0);
+ qualReverseMap[i].assign(maxLength,0);
}
}
qScoreErrorMap['a'].assign(41, 0);
map<char, vector<int> > errorForward;
- errorForward['m'].assign(1000,0);
- errorForward['s'].assign(1000,0);
- errorForward['i'].assign(1000,0);
- errorForward['d'].assign(1000,0);
- errorForward['a'].assign(1000,0);
+ errorForward['m'].assign(maxLength,0);
+ errorForward['s'].assign(maxLength,0);
+ errorForward['i'].assign(maxLength,0);
+ errorForward['d'].assign(maxLength,0);
+ errorForward['a'].assign(maxLength,0);
map<char, vector<int> > errorReverse;
- errorReverse['m'].assign(1000,0);
- errorReverse['s'].assign(1000,0);
- errorReverse['i'].assign(1000,0);
- errorReverse['d'].assign(1000,0);
- errorReverse['a'].assign(1000,0);
+ errorReverse['m'].assign(maxLength,0);
+ errorReverse['s'].assign(maxLength,0);
+ errorReverse['i'].assign(maxLength,0);
+ errorReverse['d'].assign(maxLength,0);
+ errorReverse['a'].assign(maxLength,0);
string errorChimeraFileName = queryFileName.substr(0,queryFileName.find_last_of('.')) + ".error.chimera";
RefChimeraTest chimeraTest(referenceSeqs, errorChimeraFileName);
outputNames.push_back(errorChimeraFileName); outputTypes["error.chimera"].push_back(errorChimeraFileName);
+ vector<string> megaAlignVector(numRefs, "");
+
int index = 0;
bool ignoreSeq = 0;
while(queryFile){
-
+
if (m->control_pressed) { errorSummaryFile.close(); errorSeqFile.close(); for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; }
Sequence query(queryFile);
-
+
int numParentSeqs = chimeraTest.analyzeQuery(query.getName(), query.getAligned());
int closestRefIndex = chimeraTest.getClosestRefIndex();
if(numParentSeqs > 1 && ignoreChimeras == 1) { ignoreSeq = 1; }
else { ignoreSeq = 0; }
-
Compare minCompare = getErrors(query, referenceSeqs[closestRefIndex]);
if(namesFileName != ""){
it = weights.find(query.getName());
minCompare.weight = it->second;
}
- else { minCompare.weight = 1; }
+ else{ minCompare.weight = 1; }
printErrorData(minCompare, numParentSeqs);
if(!ignoreSeq){
+
for(int i=0;i<minCompare.total;i++){
char letter = minCompare.sequence[i];
+
errorForward[letter][i] += minCompare.weight;
errorReverse[letter][minCompare.total-i-1] += minCompare.weight;
}
}
-
+
if(qualFileName != "" && reportFileName != ""){
report = ReportFile(reportFile);
- int origLength = report.getQueryLength();
+// int origLength = report.getQueryLength();
int startBase = report.getQueryStart();
int endBase = report.getQueryEnd();
- quality = QualityScores(qualFile, origLength);
+ quality = QualityScores(qualFile);
if(!ignoreSeq){
quality.updateQScoreErrorMap(qScoreErrorMap, minCompare.sequence, startBase, endBase, minCompare.weight);
quality.updateReverseMap(qualReverseMap, startBase, endBase, minCompare.weight);
}
}
-
+
if(minCompare.errorRate < threshold && !ignoreSeq){
totalBases += (minCompare.total * minCompare.weight);
totalMatches += minCompare.matches * minCompare.weight;
}
misMatchCounts[minCompare.mismatches] += minCompare.weight;
numSeqs++;
+
+ megaAlignVector[closestRefIndex] += query.getInlineSeq() + '\n';
}
-
+
index++;
- if(index % 1000 == 0){ cout << index << endl; }
+
+ if(index % 1000 == 0){ m->mothurOut(toString(index) + '\n'); }
}
queryFile.close();
errorSummaryFile.close();
printSubMatrix();
+ string megAlignmentFileName = queryFileName.substr(0,queryFileName.find_last_of('.')) + ".error.ref-query";
+ ofstream megAlignmentFile;
+ m->openOutputFile(megAlignmentFileName, megAlignmentFile);
+ outputNames.push_back(megAlignmentFileName); outputTypes["error.ref-query"].push_back(megAlignmentFileName);
+
+ for(int i=0;i<numRefs;i++){
+ megAlignmentFile << referenceSeqs[i].getInlineSeq() << endl;
+ megAlignmentFile << megaAlignVector[i] << endl;
+ }
+
+
m->mothurOutEndLine();
m->mothurOut("Output File Names: "); m->mothurOutEndLine();
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
ifstream referenceFile;
m->openInputFile(referenceFileName, referenceFile);
+ int numAmbigSeqs = 0;
+
+ int maxStartPos = 0;
+ int minEndPos = 100000;
+
while(referenceFile){
Sequence currentSeq(referenceFile);
int numAmbigs = currentSeq.getAmbigBases();
+ if(numAmbigs > 0){ numAmbigSeqs++; }
- if(numAmbigs != 0){
- m->mothurOut("Warning: " + toString(currentSeq.getName()) + " has " + toString(numAmbigs) + " ambiguous bases, these bases will be removed\n");
- currentSeq.removeAmbigBases();
- }
+ int startPos = currentSeq.getStartPos();
+ if(startPos > maxStartPos) { maxStartPos = startPos; }
+
+ int endPos = currentSeq.getEndPos();
+ if(endPos < minEndPos) { minEndPos = endPos; }
referenceSeqs.push_back(currentSeq);
m->gobble(referenceFile);
}
+ referenceFile.close();
numRefs = referenceSeqs.size();
+
+
+ for(int i=0;i<numRefs;i++){
+ referenceSeqs[i].padToPos(maxStartPos);
+ referenceSeqs[i].padFromPos(minEndPos);
+ }
+
+ if(numAmbigSeqs != 0){
+ m->mothurOut("Warning: " + toString(numAmbigSeqs) + " reference sequences have ambiguous bases, these bases will be ignored\n");
+ }
- referenceFile.close();
}
catch(exception& e) {
m->errorOut(e, "SeqErrorCommand", "getReferences");
Compare errors;
for(int i=0;i<alignLength;i++){
- if(q[i] != '.' && r[i] != '.' && (q[i] != '-' || r[i] != '-')){ // no missing data and no double gaps
+ if(r[i] != 'N' && q[i] != '.' && r[i] != '.' && (q[i] != '-' || r[i] != '-')){ // no missing data and no double gaps
started = 1;
if(q[i] == 'A'){
if(started == 1){ break; }
}
else if(q[i] != '.' && r[i] == '.'){ // query extends beyond reference
- m->mothurOut("Warning: " + toString(query.getName()) + " extend beyond " + toString(reference.getName()) + ". Ignoring the extra bases in the query\n");
if(started == 1){ break; }
}
else if(q[i] == '.' && r[i] == '.'){ // both are missing data
void SeqErrorCommand::printErrorData(Compare error, int numParentSeqs){
try {
+
errorSummaryFile << error.queryName << '\t' << error.refName << '\t' << error.weight << '\t';
errorSummaryFile << error.AA << '\t' << error.AT << '\t' << error.AG << '\t' << error.AC << '\t';
errorSummaryFile << error.TA << '\t' << error.TT << '\t' << error.TG << '\t' << error.TC << '\t';
errorSummaryFile << error.GA << '\t' << error.GT << '\t' << error.GG << '\t' << error.GC << '\t';
errorSummaryFile << error.CA << '\t' << error.CT << '\t' << error.CG << '\t' << error.CC << '\t';
errorSummaryFile << error.NA << '\t' << error.NT << '\t' << error.NG << '\t' << error.NC << '\t';
- errorSummaryFile << error.Ai << '\t' << error.Ti << '\t' << error.Gi << '\t' << error.Ci << '\t' << error.Ni << '\t' ;
+ errorSummaryFile << error.Ai << '\t' << error.Ti << '\t' << error.Gi << '\t' << error.Ci << '\t' << error.Ni << '\t';
errorSummaryFile << error.dA << '\t' << error.dT << '\t' << error.dG << '\t' << error.dC << '\t';
errorSummaryFile << error.Ai + error.Ti + error.Gi + error.Ci << '\t'; //insertions
errorSummaryFile << error.mismatches - (error.Ai + error.Ti + error.Gi + error.Ci) - (error.dA + error.dT + error.dG + error.dC) - (error.NA + error.NT + error.NG + error.NC + error.Ni) << '\t'; //substitutions
errorSummaryFile << error.NA + error.NT + error.NG + error.NC + error.Ni << '\t'; //ambiguities
errorSummaryFile << error.matches << '\t' << error.mismatches << '\t' << error.total << '\t' << error.errorRate << '\t' << numParentSeqs << endl;
-
+
errorSeqFile << '>' << error.queryName << "\tref:" << error.refName << '\n' << error.sequence << endl;
-
int a=0; int t=1; int g=2; int c=3;
int gap=4; int n=5;
+
if(numParentSeqs == 1 || ignoreChimeras == 0){
substitutionMatrix[a][a] += error.weight * error.AA;
substitutionMatrix[a][t] += error.weight * error.TA;
substitutionMatrix[a][c] += error.weight * error.CA;
substitutionMatrix[a][gap] += error.weight * error.dA;
substitutionMatrix[a][n] += error.weight * error.NA;
-
+
substitutionMatrix[t][a] += error.weight * error.AT;
substitutionMatrix[t][t] += error.weight * error.TT;
substitutionMatrix[t][g] += error.weight * error.GT;
outputNames.push_back(errorForwardFileName); outputTypes["error.forward"].push_back(errorForwardFileName);
errorForwardFile << "position\ttotalseqs\tmatch\tsubstitution\tinsertion\tdeletion\tambiguous" << endl;
- for(int i=0;i<1000;i++){
+ for(int i=0;i<maxLength;i++){
float match = (float)errorForward['m'][i];
float subst = (float)errorForward['s'][i];
float insert = (float)errorForward['i'][i];
outputNames.push_back(errorReverseFileName); outputTypes["error.reverse"].push_back(errorReverseFileName);
errorReverseFile << "position\ttotalseqs\tmatch\tsubstitution\tinsertion\tdeletion\tambiguous" << endl;
- for(int i=0;i<1000;i++){
+ for(int i=0;i<maxLength;i++){
float match = (float)errorReverse['m'][i];
float subst = (float)errorReverse['s'][i];
float insert = (float)errorReverse['i'][i];
projects / mothur.git / blobdiff