inline bool comparePriority(seqPNode first, seqPNode second) { return (first.numIdentical > second.numIdentical); }
//**********************************************************************************************************************
-PreClusterCommand::PreClusterCommand(string option){
+PreClusterCommand::PreClusterCommand(string option) {
try {
abort = false;
//check for required parameters
fastafile = validParameter.validFile(parameters, "fasta", true);
- if (fastafile == "not found") { mothurOut("fasta is a required parameter for the pre.cluster command."); mothurOutEndLine(); abort = true; }
+ if (fastafile == "not found") { m->mothurOut("fasta is a required parameter for the pre.cluster command."); m->mothurOutEndLine(); abort = true; }
else if (fastafile == "not open") { abort = true; }
//if the user changes the output directory command factory will send this info to us in the output parameter
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "PreClusterCommand");
+ m->errorOut(e, "PreClusterCommand", "PreClusterCommand");
exit(1);
}
}
void PreClusterCommand::help(){
try {
- mothurOut("The pre.cluster command groups sequences that are within a given number of base mismatches.\n");
- mothurOut("The pre.cluster command outputs a new fasta and name file.\n");
- mothurOut("The pre.cluster command parameters are fasta, names and diffs. The fasta parameter is required. \n");
- mothurOut("The names parameter allows you to give a list of seqs that are identical. This file is 2 columns, first column is name or representative sequence, second column is a list of its identical sequences separated by commas.\n");
- mothurOut("The diffs parameter allows you to specify maximum number of mismatched bases allowed between sequences in a grouping. The default is 1.\n");
- mothurOut("The pre.cluster command should be in the following format: \n");
- mothurOut("pre.cluster(fasta=yourFastaFile, names=yourNamesFile, diffs=yourMaxDiffs) \n");
- mothurOut("Example pre.cluster(fasta=amazon.fasta, diffs=2).\n");
- mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n");
+ m->mothurOut("The pre.cluster command groups sequences that are within a given number of base mismatches.\n");
+ m->mothurOut("The pre.cluster command outputs a new fasta and name file.\n");
+ m->mothurOut("The pre.cluster command parameters are fasta, names and diffs. The fasta parameter is required. \n");
+ m->mothurOut("The names parameter allows you to give a list of seqs that are identical. This file is 2 columns, first column is name or representative sequence, second column is a list of its identical sequences separated by commas.\n");
+ m->mothurOut("The diffs parameter allows you to specify maximum number of mismatched bases allowed between sequences in a grouping. The default is 1.\n");
+ m->mothurOut("The pre.cluster command should be in the following format: \n");
+ m->mothurOut("pre.cluster(fasta=yourFastaFile, names=yourNamesFile, diffs=yourMaxDiffs) \n");
+ m->mothurOut("Example pre.cluster(fasta=amazon.fasta, diffs=2).\n");
+ m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n");
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "help");
+ m->errorOut(e, "PreClusterCommand", "help");
exit(1);
}
}
//reads fasta file and return number of seqs
int numSeqs = readFASTA(); //fills alignSeqs and makes all seqs active
-
- if (numSeqs == 0) { mothurOut("Error reading fasta file...please correct."); mothurOutEndLine(); return 0; }
- if (diffs > length) { mothurOut("Error: diffs is greater than your sequence length."); mothurOutEndLine(); return 0; }
- //clear sizes since you only needed this info to build the alignSeqs seqPNode structs
-// sizes.clear();
+ if (m->control_pressed) { return 0; }
+ if (numSeqs == 0) { m->mothurOut("Error reading fasta file...please correct."); m->mothurOutEndLine(); return 0; }
+ if (diffs > length) { m->mothurOut("Error: diffs is greater than your sequence length."); m->mothurOutEndLine(); return 0; }
+
+ string fileroot = outputDir + getRootName(getSimpleName(fastafile));
+ string newFastaFile = fileroot + "precluster" + getExtension(fastafile);
+ string newNamesFile = fileroot + "precluster.names";
+ ofstream outFasta;
+ ofstream outNames;
+
+ openOutputFile(newFastaFile, outFasta);
+ openOutputFile(newNamesFile, outNames);
+
//sort seqs by number of identical seqs
- sort(alignSeqs.begin(), alignSeqs.end(), comparePriority);
-
- int count = 0;
+ alignSeqs.sort(comparePriority);
+ int count = 0;
+ int i = 0;
//think about running through twice...
- for (int i = 0; i < numSeqs; i++) {
-
- //are you active
- // itActive = active.find(alignSeqs[i].seq.getName());
+ list<seqPNode>::iterator itList;
+ list<seqPNode>::iterator itList2;
+ for (itList = alignSeqs.begin(); itList != alignSeqs.end();) {
- if (alignSeqs[i].active) { //this sequence has not been merged yet
+ //try to merge it with all smaller seqs
+ for (itList2 = alignSeqs.begin(); itList2 != alignSeqs.end();) {
+
+ if (m->control_pressed) { outFasta.close(); outNames.close(); remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
- //try to merge it with all smaller seqs
- for (int j = i+1; j < numSeqs; j++) {
- if (alignSeqs[j].active) { //this sequence has not been merged yet
- //are you within "diff" bases
- int mismatch = calcMisMatches(alignSeqs[i].seq.getAligned(), alignSeqs[j].seq.getAligned());
+
+ if (itList->seq.getName() != itList2->seq.getName()) { //you don't want to merge with yourself
+ //are you within "diff" bases
+
+ int mismatch = calcMisMatches((*itList).seq.getAligned(), (*itList2).seq.getAligned());
+
+ if (mismatch <= diffs) {
+ //merge
+ (*itList).names += ',' + (*itList2).names;
+ (*itList).numIdentical += (*itList2).numIdentical;
- if (mismatch <= diffs) {
- //merge
- alignSeqs[i].names += ',' + alignSeqs[j].names;
- alignSeqs[i].numIdentical += alignSeqs[j].numIdentical;
+ itList2 = alignSeqs.erase(itList2); //itList2--;
+ count++;
+ }else{ itList2++; }
+ }else{ itList2++; }
- alignSeqs[j].active = 0;
- alignSeqs[j].numIdentical = 0;
- count++;
- }
- }//end if j active
- }//end if i != j
+ }
+
+ //ouptut this sequence
+ printData(outFasta, outNames, (*itList));
+
+ //remove sequence
+ itList = alignSeqs.erase(itList);
- //remove from active list
- alignSeqs[i].active = 0;
- }//end if active i
- if(i % 100 == 0) { cout << i << '\t' << numSeqs - count << '\t' << count << endl; }
+ i++;
+
+ if(i % 100 == 0) { m->mothurOut(toString(i) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
}
+
+ if(i % 100 != 0) { m->mothurOut(toString(i) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
- string fileroot = outputDir + getRootName(getSimpleName(fastafile));
+ outFasta.close();
+ outNames.close();
- string newFastaFile = fileroot + "precluster" + getExtension(fastafile);
- string newNamesFile = fileroot + "precluster.names";
+ if (m->control_pressed) { remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
+
+ m->mothurOut("Total number of sequences before precluster was " + toString(numSeqs) + "."); m->mothurOutEndLine();
+ m->mothurOut("pre.cluster removed " + toString(count) + " sequences."); m->mothurOutEndLine();
+ if (m->control_pressed) { remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
- mothurOut("Total number of sequences before precluster was " + toString(alignSeqs.size()) + "."); mothurOutEndLine();
- mothurOut("pre.cluster removed " + toString(count) + " sequences."); mothurOutEndLine();
- printData(newFastaFile, newNamesFile);
+ m->mothurOutEndLine();
+ m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+ m->mothurOut(newFastaFile); m->mothurOutEndLine();
+ m->mothurOut(newNamesFile); m->mothurOutEndLine();
+ m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "execute");
+ m->errorOut(e, "PreClusterCommand", "execute");
exit(1);
}
}
length = 0;
while (!inFasta.eof()) {
-
+
+ if (m->control_pressed) { inFasta.close(); return 0; }
+
//inNames >> firstCol >> secondCol;
//nameString = secondCol;
if (namefile != "") {
itSize = sizes.find(seq.getName());
- if (itSize == sizes.end()) { mothurOut(seq.getName() + " is not in your names file, please correct."); mothurOutEndLine(); exit(1); }
+ if (itSize == sizes.end()) { m->mothurOut(seq.getName() + " is not in your names file, please correct."); m->mothurOutEndLine(); exit(1); }
else{
seqPNode tempNode(itSize->second, seq, names[seq.getName()]);
alignSeqs.push_back(tempNode);
- if (seq.getAligned().length() > length) { length = alignSeqs[0].seq.getAligned().length(); }
+ if (seq.getAligned().length() > length) { length = seq.getAligned().length(); }
}
}else { //no names file, you are identical to yourself
seqPNode tempNode(1, seq, seq.getName());
alignSeqs.push_back(tempNode);
- if (seq.getAligned().length() > length) { length = alignSeqs[0].seq.getAligned().length(); }
+ if (seq.getAligned().length() > length) { length = seq.getAligned().length(); }
}
}
}
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "readFASTA");
+ m->errorOut(e, "PreClusterCommand", "readFASTA");
exit(1);
}
}
return numBad;
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "calcMisMatches");
+ m->errorOut(e, "PreClusterCommand", "calcMisMatches");
exit(1);
}
}
/**************************************************************************************************/
-void PreClusterCommand::printData(string newfasta, string newname){
+void PreClusterCommand::printData(ofstream& outFasta, ofstream& outNames, seqPNode thisSeq){
try {
- ofstream outFasta;
- ofstream outNames;
-
- openOutputFile(newfasta, outFasta);
- openOutputFile(newname, outNames);
-
-
- for (int i = 0; i < alignSeqs.size(); i++) {
- if (alignSeqs[i].numIdentical != 0) {
- alignSeqs[i].seq.printSequence(outFasta);
- outNames << alignSeqs[i].seq.getName() << '\t' << alignSeqs[i].names << endl;
- }
- }
-
- outFasta.close();
- outNames.close();
-
+ thisSeq.seq.printSequence(outFasta);
+ outNames << thisSeq.seq.getName() << '\t' << thisSeq.names << endl;
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "printData");
+ m->errorOut(e, "PreClusterCommand", "printData");
exit(1);
}
}
-
/**************************************************************************************************/
void PreClusterCommand::readNameFile(){
in.close();
}
catch(exception& e) {
- errorOut(e, "PreClusterCommand", "readNameFile");
+ m->errorOut(e, "PreClusterCommand", "readNameFile");
exit(1);
}
}
projects / mothur.git / blobdiff