*/
#include "preclustercommand.h"
-
-//**********************************************************************************************************************
-inline bool comparePriority(seqPNode first, seqPNode second) { return (first.numIdentical > second.numIdentical); }
+#include "deconvolutecommand.h"
//**********************************************************************************************************************
vector<string> PreClusterCommand::setParameters(){
try {
CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfasta);
CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
+ CommandParameter pgroup("group", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pgroup);
CommandParameter pdiffs("diffs", "Number", "", "0", "", "", "",false,false); parameters.push_back(pdiffs);
+ CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
helpString += "The pre.cluster command outputs a new fasta and name file.\n";
helpString += "The pre.cluster command parameters are fasta, names and diffs. The fasta parameter is required. \n";
helpString += "The names parameter allows you to give a list of seqs that are identical. This file is 2 columns, first column is name or representative sequence, second column is a list of its identical sequences separated by commas.\n";
+ helpString += "The group parameter allows you to provide a group file so you can cluster by group. \n";
helpString += "The diffs parameter allows you to specify maximum number of mismatched bases allowed between sequences in a grouping. The default is 1.\n";
helpString += "The pre.cluster command should be in the following format: \n";
helpString += "pre.cluster(fasta=yourFastaFile, names=yourNamesFile, diffs=yourMaxDiffs) \n";
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
outputTypes["name"] = tempOutNames;
+ outputTypes["map"] = tempOutNames;
}
catch(exception& e) {
m->errorOut(e, "PreClusterCommand", "PreClusterCommand");
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
+ else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
vector<string> myArray = setParameters();
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
outputTypes["name"] = tempOutNames;
+ outputTypes["map"] = tempOutNames;
//if the user changes the input directory command factory will send this info to us in the output parameter
string inputDir = validParameter.validFile(parameters, "inputdir", false);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["name"] = inputDir + it->second; }
}
+
+ it = parameters.find("group");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["group"] = inputDir + it->second; }
+ }
}
//check for required parameters
else { m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
}
else if (fastafile == "not open") { abort = true; }
+ else { m->setFastaFile(fastafile); }
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
// ...at some point should added some additional type checking...
namefile = validParameter.validFile(parameters, "name", true);
if (namefile == "not found") { namefile = ""; }
- else if (namefile == "not open") { abort = true; }
- else { readNameFile(); }
+ else if (namefile == "not open") { namefile = ""; abort = true; }
+ else { m->setNameFile(namefile); }
+
+ groupfile = validParameter.validFile(parameters, "group", true);
+ if (groupfile == "not found") { groupfile = ""; bygroup = false; }
+ else if (groupfile == "not open") { abort = true; groupfile = ""; }
+ else { m->setGroupFile(groupfile); bygroup = true; }
- string temp = validParameter.validFile(parameters, "diffs", false); if(temp == "not found"){ temp = "1"; }
- convert(temp, diffs);
+ string temp = validParameter.validFile(parameters, "diffs", false); if(temp == "not found"){ temp = "1"; }
+ m->mothurConvert(temp, diffs);
+
+ temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = m->getProcessors(); }
+ m->setProcessors(temp);
+ m->mothurConvert(temp, processors);
+
+ if (namefile == "") {
+ vector<string> files; files.push_back(fastafile);
+ parser.getNameFile(files);
+ }
}
}
int start = time(NULL);
- //reads fasta file and return number of seqs
- int numSeqs = readFASTA(); //fills alignSeqs and makes all seqs active
+ string fileroot = outputDir + m->getRootName(m->getSimpleName(fastafile));
+ string newFastaFile = fileroot + "precluster" + m->getExtension(fastafile);
+ string newNamesFile = fileroot + "precluster.names";
+ string newMapFile = fileroot + "precluster.map"; //add group name if by group
+ outputNames.push_back(newFastaFile); outputTypes["fasta"].push_back(newFastaFile);
+ outputNames.push_back(newNamesFile); outputTypes["name"].push_back(newNamesFile);
- if (m->control_pressed) { return 0; }
-
- if (numSeqs == 0) { m->mothurOut("Error reading fasta file...please correct."); m->mothurOutEndLine(); return 0; }
- if (diffs > length) { m->mothurOut("Error: diffs is greater than your sequence length."); m->mothurOutEndLine(); return 0; }
- //clear sizes since you only needed this info to build the alignSeqs seqPNode structs
-// sizes.clear();
+ if (bygroup) {
+ //clear out old files
+ ofstream outFasta; m->openOutputFile(newFastaFile, outFasta); outFasta.close();
+ ofstream outNames; m->openOutputFile(newNamesFile, outNames); outNames.close();
+ newMapFile = fileroot + "precluster.";
+
+ //parse fasta and name file by group
+ SequenceParser* parser;
+ if (namefile != "") { parser = new SequenceParser(groupfile, fastafile, namefile); }
+ else { parser = new SequenceParser(groupfile, fastafile); }
+
+ vector<string> groups = parser->getNamesOfGroups();
+
+ if(processors == 1) { driverGroups(parser, newFastaFile, newNamesFile, newMapFile, 0, groups.size(), groups); }
+ else { createProcessesGroups(parser, newFastaFile, newNamesFile, newMapFile, groups); }
+
+ delete parser;
+
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+ //run unique.seqs for deconvolute results
+ string inputString = "fasta=" + newFastaFile;
+ if (namefile != "") { inputString += ", name=" + newNamesFile; }
+ m->mothurOutEndLine();
+ m->mothurOut("/******************************************/"); m->mothurOutEndLine();
+ m->mothurOut("Running command: unique.seqs(" + inputString + ")"); m->mothurOutEndLine();
+ m->mothurCalling = true;
+
+ Command* uniqueCommand = new DeconvoluteCommand(inputString);
+ uniqueCommand->execute();
+
+ map<string, vector<string> > filenames = uniqueCommand->getOutputFiles();
+
+ delete uniqueCommand;
+ m->mothurCalling = false;
+ m->mothurOut("/******************************************/"); m->mothurOutEndLine();
+
+ m->renameFile(filenames["fasta"][0], newFastaFile);
+ m->renameFile(filenames["name"][0], newNamesFile);
+
+ m->mothurOut("It took " + toString(time(NULL) - start) + " secs to run pre.cluster."); m->mothurOutEndLine();
+
+ }else {
+ if (namefile != "") { readNameFile(); }
+
+ //reads fasta file and return number of seqs
+ int numSeqs = readFASTA(); //fills alignSeqs and makes all seqs active
+
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+ if (numSeqs == 0) { m->mothurOut("Error reading fasta file...please correct."); m->mothurOutEndLine(); return 0; }
+ if (diffs > length) { m->mothurOut("Error: diffs is greater than your sequence length."); m->mothurOutEndLine(); return 0; }
+
+ int count = process(newMapFile);
+ outputNames.push_back(newMapFile); outputTypes["map"].push_back(newMapFile);
+
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+ m->mothurOut("Total number of sequences before precluster was " + toString(alignSeqs.size()) + "."); m->mothurOutEndLine();
+ m->mothurOut("pre.cluster removed " + toString(count) + " sequences."); m->mothurOutEndLine(); m->mothurOutEndLine();
+ printData(newFastaFile, newNamesFile);
+
+ m->mothurOut("It took " + toString(time(NULL) - start) + " secs to cluster " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
+ }
+
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+ m->mothurOutEndLine();
+ m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
+ m->mothurOutEndLine();
+
+ //set fasta file as new current fastafile
+ string current = "";
+ itTypes = outputTypes.find("fasta");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
+ }
+
+ itTypes = outputTypes.find("name");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setNameFile(current); }
+ }
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "PreClusterCommand", "execute");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
+int PreClusterCommand::createProcessesGroups(SequenceParser* parser, string newFName, string newNName, string newMFile, vector<string> groups) {
+ try {
+
+ vector<int> processIDS;
+ int process = 1;
+ int num = 0;
+
+ //sanity check
+ if (groups.size() < processors) { processors = groups.size(); }
+
+ //divide the groups between the processors
+ vector<linePair> lines;
+ int numGroupsPerProcessor = groups.size() / processors;
+ for (int i = 0; i < processors; i++) {
+ int startIndex = i * numGroupsPerProcessor;
+ int endIndex = (i+1) * numGroupsPerProcessor;
+ if(i == (processors - 1)){ endIndex = groups.size(); }
+ lines.push_back(linePair(startIndex, endIndex));
+ }
+
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+
+ //loop through and create all the processes you want
+ while (process != processors) {
+ int pid = fork();
+
+ if (pid > 0) {
+ processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later
+ process++;
+ }else if (pid == 0){
+ outputNames.clear();
+ num = driverGroups(parser, newFName + toString(getpid()) + ".temp", newNName + toString(getpid()) + ".temp", newMFile, lines[process].start, lines[process].end, groups);
+
+ string tempFile = toString(getpid()) + ".outputNames.temp";
+ ofstream outTemp;
+ m->openOutputFile(tempFile, outTemp);
+
+ outTemp << outputNames.size();
+ for (int i = 0; i < outputNames.size(); i++) { outTemp << outputNames[i] << endl; }
+ outTemp.close();
+
+ exit(0);
+ }else {
+ m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
+ for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+ exit(0);
+ }
+ }
+
+ //do my part
+ num = driverGroups(parser, newFName, newNName, newMFile, lines[0].start, lines[0].end, groups);
+
+ //force parent to wait until all the processes are done
+ for (int i=0;i<processIDS.size();i++) {
+ int temp = processIDS[i];
+ wait(&temp);
+ }
+
+ for (int i = 0; i < processIDS.size(); i++) {
+ string tempFile = toString(processIDS[i]) + ".outputNames.temp";
+ ifstream intemp;
+ m->openInputFile(tempFile, intemp);
+
+ int num;
+ intemp >> num;
+ for (int k = 0; k < num; k++) {
+ string name = "";
+ intemp >> name; m->gobble(intemp);
+
+ outputNames.push_back(name); outputTypes["map"].push_back(name);
+ }
+ intemp.close();
+ m->mothurRemove(tempFile);
+ }
+#else
+
+ //////////////////////////////////////////////////////////////////////////////////////////////////////
+ //Windows version shared memory, so be careful when passing variables through the preClusterData struct.
+ //Above fork() will clone, so memory is separate, but that's not the case with windows,
+ //////////////////////////////////////////////////////////////////////////////////////////////////////
+
+ vector<preClusterData*> pDataArray;
+ DWORD dwThreadIdArray[processors-1];
+ HANDLE hThreadArray[processors-1];
+
+ //Create processor worker threads.
+ for( int i=1; i<processors; i++ ){
+ // Allocate memory for thread data.
+ string extension = toString(i) + ".temp";
+
+ preClusterData* tempPreCluster = new preClusterData(fastafile, namefile, groupfile, (newFName+extension), (newNName+extension), newMFile, groups, m, lines[i].start, lines[i].end, diffs, i);
+ pDataArray.push_back(tempPreCluster);
+ processIDS.push_back(i);
+
+ //MySeqSumThreadFunction is in header. It must be global or static to work with the threads.
+ //default security attributes, thread function name, argument to thread function, use default creation flags, returns the thread identifier
+ hThreadArray[i-1] = CreateThread(NULL, 0, MyPreclusterThreadFunction, pDataArray[i-1], 0, &dwThreadIdArray[i-1]);
+ }
+
+
+ //using the main process as a worker saves time and memory
+ num = driverGroups(parser, newFName, newNName, newMFile, lines[0].start, lines[0].end, groups);
+
+ //Wait until all threads have terminated.
+ WaitForMultipleObjects(processors-1, hThreadArray, TRUE, INFINITE);
+
+ //Close all thread handles and free memory allocations.
+ for(int i=0; i < pDataArray.size(); i++){
+ for (int j = 0; j < pDataArray[i]->mapFileNames.size(); j++) {
+ outputNames.push_back(pDataArray[i]->mapFileNames[j]); outputTypes["map"].push_back(pDataArray[i]->mapFileNames[j]);
+ }
+ CloseHandle(hThreadArray[i]);
+ delete pDataArray[i];
+ }
+
+#endif
+
+ //append output files
+ for(int i=0;i<processIDS.size();i++){
+ //newFName = m->getFullPathName(".\\" + newFName);
+ //newNName = m->getFullPathName(".\\" + newNName);
+
+ m->appendFiles((newFName + toString(processIDS[i]) + ".temp"), newFName);
+ m->mothurRemove((newFName + toString(processIDS[i]) + ".temp"));
+
+ m->appendFiles((newNName + toString(processIDS[i]) + ".temp"), newNName);
+ m->mothurRemove((newNName + toString(processIDS[i]) + ".temp"));
+ }
+
+ return num;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "PreClusterCommand", "createProcessesGroups");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
+int PreClusterCommand::driverGroups(SequenceParser* parser, string newFFile, string newNFile, string newMFile, int start, int end, vector<string> groups){
+ try {
+
+ int numSeqs = 0;
+
+ //precluster each group
+ for (int i = start; i < end; i++) {
+
+ start = time(NULL);
+
+ if (m->control_pressed) { return 0; }
+
+ m->mothurOutEndLine(); m->mothurOut("Processing group " + groups[i] + ":"); m->mothurOutEndLine();
+
+ map<string, string> thisNameMap;
+ if (namefile != "") { thisNameMap = parser->getNameMap(groups[i]); }
+ vector<Sequence> thisSeqs = parser->getSeqs(groups[i]);
+
+ //fill alignSeqs with this groups info.
+ numSeqs = loadSeqs(thisNameMap, thisSeqs);
+
+ if (m->control_pressed) { return 0; }
+
+ if (diffs > length) { m->mothurOut("Error: diffs is greater than your sequence length."); m->mothurOutEndLine(); m->control_pressed = true; return 0; }
+
+ int count = process(newMFile+groups[i]+".map");
+ outputNames.push_back(newMFile+groups[i]+".map"); outputTypes["map"].push_back(newMFile+groups[i]+".map");
+
+ if (m->control_pressed) { return 0; }
+
+ m->mothurOut("Total number of sequences before pre.cluster was " + toString(alignSeqs.size()) + "."); m->mothurOutEndLine();
+ m->mothurOut("pre.cluster removed " + toString(count) + " sequences."); m->mothurOutEndLine(); m->mothurOutEndLine();
+ printData(newFFile, newNFile);
+
+ m->mothurOut("It took " + toString(time(NULL) - start) + " secs to cluster " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
+
+ }
+
+ return numSeqs;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "PreClusterCommand", "driverGroups");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
+int PreClusterCommand::process(string newMapFile){
+ try {
+ ofstream out;
+ m->openOutputFile(newMapFile, out);
+
//sort seqs by number of identical seqs
sort(alignSeqs.begin(), alignSeqs.end(), comparePriority);
-
+
int count = 0;
-
+ int numSeqs = alignSeqs.size();
+
//think about running through twice...
for (int i = 0; i < numSeqs; i++) {
if (alignSeqs[i].active) { //this sequence has not been merged yet
+ string chunk = alignSeqs[i].seq.getName() + "\t" + toString(alignSeqs[i].numIdentical) + "\t" + toString(0) + "\t" + alignSeqs[i].seq.getAligned() + "\n";
+
//try to merge it with all smaller seqs
for (int j = i+1; j < numSeqs; j++) {
- if (m->control_pressed) { return 0; }
+ if (m->control_pressed) { out.close(); return 0; }
if (alignSeqs[j].active) { //this sequence has not been merged yet
//are you within "diff" bases
//merge
alignSeqs[i].names += ',' + alignSeqs[j].names;
alignSeqs[i].numIdentical += alignSeqs[j].numIdentical;
-
+
+ chunk += alignSeqs[j].seq.getName() + "\t" + toString(alignSeqs[j].numIdentical) + "\t" + toString(mismatch) + "\t" + alignSeqs[j].seq.getAligned() + "\n";
+
alignSeqs[j].active = 0;
alignSeqs[j].numIdentical = 0;
count++;
}
}//end if j active
- }//end if i != j
-
- //remove from active list
+ }//end for loop j
+
+ //remove from active list
alignSeqs[i].active = 0;
+ out << "ideal_seq_" << (i+1) << '\t' << alignSeqs[i].numIdentical << endl << chunk << endl;;
+
}//end if active i
if(i % 100 == 0) { m->mothurOut(toString(i) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
}
+ out.close();
- if(numSeqs % 100 != 0) { m->mothurOut(toString(numSeqs) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
-
-
- string fileroot = outputDir + m->getRootName(m->getSimpleName(fastafile));
-
- string newFastaFile = fileroot + "precluster" + m->getExtension(fastafile);
- string newNamesFile = fileroot + "precluster.names";
-
- if (m->control_pressed) { return 0; }
-
- m->mothurOut("Total number of sequences before precluster was " + toString(alignSeqs.size()) + "."); m->mothurOutEndLine();
- m->mothurOut("pre.cluster removed " + toString(count) + " sequences."); m->mothurOutEndLine(); m->mothurOutEndLine();
- printData(newFastaFile, newNamesFile);
+ if(numSeqs % 100 != 0) { m->mothurOut(toString(numSeqs) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
- m->mothurOut("It took " + toString(time(NULL) - start) + " secs to cluster " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
-
- if (m->control_pressed) { remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
-
- m->mothurOutEndLine();
- m->mothurOut("Output File Names: "); m->mothurOutEndLine();
- m->mothurOut(newFastaFile); m->mothurOutEndLine(); outputNames.push_back(newFastaFile); outputTypes["fasta"].push_back(newFastaFile);
- m->mothurOut(newNamesFile); m->mothurOutEndLine(); outputNames.push_back(newNamesFile); outputTypes["name"].push_back(newNamesFile);
- m->mothurOutEndLine();
-
- //set fasta file as new current fastafile
- string current = "";
- itTypes = outputTypes.find("fasta");
- if (itTypes != outputTypes.end()) {
- if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
- }
-
- itTypes = outputTypes.find("name");
- if (itTypes != outputTypes.end()) {
- if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setNameFile(current); }
- }
-
- return 0;
+ return count;
}
catch(exception& e) {
- m->errorOut(e, "PreClusterCommand", "execute");
+ m->errorOut(e, "PreClusterCommand", "process");
exit(1);
}
}
-
/**************************************************************************************************/
-
-//this requires the names and fasta file to be in the same order
-
int PreClusterCommand::readFASTA(){
try {
//ifstream inNames;
m->openInputFile(fastafile, inFasta);
//string firstCol, secondCol, nameString;
- length = 0;
+ set<int> lengths;
while (!inFasta.eof()) {
else{
seqPNode tempNode(itSize->second, seq, names[seq.getName()]);
alignSeqs.push_back(tempNode);
- if (seq.getAligned().length() > length) { length = seq.getAligned().length(); }
+ lengths.insert(seq.getAligned().length());
}
}else { //no names file, you are identical to yourself
seqPNode tempNode(1, seq, seq.getName());
alignSeqs.push_back(tempNode);
- if (seq.getAligned().length() > length) { length = seq.getAligned().length(); }
+ lengths.insert(seq.getAligned().length());
}
}
}
inFasta.close();
//inNames.close();
+
+ if (lengths.size() > 1) { m->control_pressed = true; m->mothurOut("[ERROR]: your sequences are not all the same length. pre.cluster requires sequences to be aligned."); m->mothurOutEndLine(); }
+ else if (lengths.size() == 1) { length = *(lengths.begin()); }
+
return alignSeqs.size();
}
exit(1);
}
}
-
+/**************************************************************************************************/
+int PreClusterCommand::loadSeqs(map<string, string>& thisName, vector<Sequence>& thisSeqs){
+ try {
+ set<int> lengths;
+ alignSeqs.clear();
+ map<string, string>::iterator it;
+ bool error = false;
+
+ for (int i = 0; i < thisSeqs.size(); i++) {
+
+ if (m->control_pressed) { return 0; }
+
+ if (namefile != "") {
+ it = thisName.find(thisSeqs[i].getName());
+
+ //should never be true since parser checks for this
+ if (it == thisName.end()) { m->mothurOut(thisSeqs[i].getName() + " is not in your names file, please correct."); m->mothurOutEndLine(); error = true; }
+ else{
+ //get number of reps
+ int numReps = 1;
+ for(int j=0;j<(it->second).length();j++){
+ if((it->second)[j] == ','){ numReps++; }
+ }
+
+ seqPNode tempNode(numReps, thisSeqs[i], it->second);
+ alignSeqs.push_back(tempNode);
+ lengths.insert(thisSeqs[i].getAligned().length());
+ }
+ }else { //no names file, you are identical to yourself
+ seqPNode tempNode(1, thisSeqs[i], thisSeqs[i].getName());
+ alignSeqs.push_back(tempNode);
+ lengths.insert(thisSeqs[i].getAligned().length());
+ }
+ }
+
+ if (lengths.size() > 1) { error = true; m->mothurOut("[ERROR]: your sequences are not all the same length. pre.cluster requires sequences to be aligned."); m->mothurOutEndLine(); }
+ else if (lengths.size() == 1) { length = *(lengths.begin()); }
+
+ //sanity check
+ if (error) { m->control_pressed = true; }
+
+ thisSeqs.clear();
+
+ return alignSeqs.size();
+ }
+
+ catch(exception& e) {
+ m->errorOut(e, "PreClusterCommand", "loadSeqs");
+ exit(1);
+ }
+}
+
/**************************************************************************************************/
int PreClusterCommand::calcMisMatches(string seq1, string seq2){
ofstream outFasta;
ofstream outNames;
- m->openOutputFile(newfasta, outFasta);
- m->openOutputFile(newname, outNames);
-
+ if (bygroup) {
+ m->openOutputFileAppend(newfasta, outFasta);
+ m->openOutputFileAppend(newname, outNames);
+ }else {
+ m->openOutputFile(newfasta, outFasta);
+ m->openOutputFile(newname, outNames);
+ }
for (int i = 0; i < alignSeqs.size(); i++) {
if (alignSeqs[i].numIdentical != 0) {