vector<string> ParseFastaQCommand::setParameters(){
try {
CommandParameter pfastq("fastq", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfastq);
+ CommandParameter pfasta("fasta", "Bool", "", "T", "", "", "",false,false); parameters.push_back(pfasta);
+ CommandParameter pqual("qfile", "Bool", "", "T", "", "", "",false,false); parameters.push_back(pqual);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
try {
string helpString = "";
helpString += "The fastq.info command reads a fastq file and creates a fasta and quality file.\n";
- helpString += "The fastq.info command parameter is fastq, and it is required.\n";
+ helpString += "The fastq.info command parameters are fastq, fasta and qfile; fastq is required.\n";
helpString += "The fastq.info command should be in the following format: fastq.info(fastaq=yourFastaQFile).\n";
+ helpString += "The fasta parameter allows you to indicate whether you want a fasta file generated. Default=T.\n";
+ helpString += "The qfile parameter allows you to indicate whether you want a quality file generated. Default=T.\n";
helpString += "Example fastq.info(fastaq=test.fastaq).\n";
helpString += "Note: No spaces between parameter labels (i.e. fastq), '=' and yourFastQFile.\n";
return helpString;
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = m->hasPath(fastaQFile); }
+
+ string temp;
+ temp = validParameter.validFile(parameters, "fasta", false); if(temp == "not found"){ temp = "T"; }
+ fasta = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "qfile", false); if(temp == "not found"){ temp = "T"; }
+ qual = m->isTrue(temp);
+
+ if ((!fasta) && (!qual)) { m->mothurOut("[ERROR]: no outputs selected. Aborting."); m->mothurOutEndLine(); abort=true; }
}
}
string fastaFile = outputDir + m->getRootName(m->getSimpleName(fastaQFile)) + "fasta";
string qualFile = outputDir + m->getRootName(m->getSimpleName(fastaQFile)) + "qual";
ofstream outFasta, outQual;
- m->openOutputFile(fastaFile, outFasta); outputNames.push_back(fastaFile); outputTypes["fasta"].push_back(fastaFile);
- m->openOutputFile(qualFile, outQual); outputNames.push_back(qualFile); outputTypes["qfile"].push_back(qualFile);
+
+ if (fasta) { m->openOutputFile(fastaFile, outFasta); outputNames.push_back(fastaFile); outputTypes["fasta"].push_back(fastaFile); }
+ if (qual) { m->openOutputFile(qualFile, outQual); outputNames.push_back(qualFile); outputTypes["qfile"].push_back(qualFile); }
ifstream in;
m->openInputFile(fastaQFile, in);
while (!in.eof()) {
+
+ if (m->control_pressed) { break; }
//read sequence name
string name = m->getline(in); m->gobble(in);
else { name2 = name2.substr(1); }
//read quality scores
- string qual = m->getline(in); m->gobble(in);
- if (qual == "") { m->mothurOut("[ERROR]: missing quality for " + name2); m->mothurOutEndLine(); m->control_pressed = true; break; }
+ string quality = m->getline(in); m->gobble(in);
+ if (quality == "") { m->mothurOut("[ERROR]: missing quality for " + name2); m->mothurOutEndLine(); m->control_pressed = true; break; }
//sanity check sequence length and number of quality scores match
if (name2 != "") { if (name != name2) { m->mothurOut("[ERROR]: names do not match. read " + name + " for fasta and " + name2 + " for quality."); m->mothurOutEndLine(); m->control_pressed = true; break; } }
- if (qual.length() != sequence.length()) { m->mothurOut("[ERROR]: lengths do not match. read " + toString(sequence.length()) + " characters for fasta and " + toString(qual.length()) + " characters for quality scores."); m->mothurOutEndLine(); m->control_pressed = true; break; }
-
- //convert quality scores
- vector<int> qualScores = convertQual(qual);
+ if (quality.length() != sequence.length()) { m->mothurOut("[ERROR]: Lengths do not match for sequence " + name + ". Read " + toString(sequence.length()) + " characters for fasta and " + toString(quality.length()) + " characters for quality scores."); m->mothurOutEndLine(); m->control_pressed = true; break; }
//print sequence info to files
- outFasta << ">" << name << endl << sequence << endl;
+ if (fasta) { outFasta << ">" << name << endl << sequence << endl; }
- outQual << ">" << name << endl;
- for (int i = 0; i < qualScores.size(); i++) { outQual << qualScores[i] << " "; }
- outQual << endl;
+ if (qual) {
+ vector<int> qualScores = convertQual(quality);
+ outQual << ">" << name << endl;
+ for (int i = 0; i < qualScores.size(); i++) { outQual << qualScores[i] << " "; }
+ outQual << endl;
+ }
}
in.close();
- outFasta.close();
- outQual.close();
+ if (fasta) { outFasta.close(); }
+ if (qual) { outQual.close(); }
if (m->control_pressed) { outputTypes.clear(); m->mothurRemove(fastaFile); m->mothurRemove(qualFile); return 0; }