#include "getoturepcommand.h"
//**********************************************************************************************************************
-GetOTURepCommand::GetOTURepCommand(){
+GetOTURepCommand::GetOTURepCommand(string option){
try{
globaldata = GlobalData::getInstance();
-
- if(globaldata->gSparseMatrix != NULL) { matrix = new SparseMatrix(*globaldata->gSparseMatrix); }
+ abort = false;
+ allLines = 1;
+ lines.clear();
+ labels.clear();
- //listOfNames bin 0 = first name read in distance matrix, listOfNames bin 1 = second name read in distance matrix
- if(globaldata->gListVector != NULL) {
- listOfNames = new ListVector(*globaldata->gListVector);
+ //allow user to run help
+ if (option == "help") {
+ help(); abort = true;
+ } else {
+ //valid paramters for this command
+ string Array[] = {"fasta","list","line","label","name", "group"};
+ vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+
+ OptionParser parser(option);
+ map<string, string> parameters = parser.getParameters();
- //map names to rows in sparsematrix
- for (int i = 0; i < listOfNames->size(); i++) {
- nameToIndex[listOfNames->get(i)] = i;
+ ValidParameters validParameter;
+
+ //check to make sure all parameters are valid for command
+ for (map<string, string>::iterator it = parameters.begin(); it != parameters.end(); it++) {
+ if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
- }else { cout << "error" << endl; }
-
+
+ //make sure the user has already run the read.otu command
+ if ((globaldata->gSparseMatrix == NULL) || (globaldata->gListVector == NULL)) {
+ mothurOut("Before you use the get.oturep command, you first need to read in a distance matrix."); mothurOutEndLine();
+ abort = true;
+ }
+
+ //check for required parameters
+ fastafile = validParameter.validFile(parameters, "fasta", true);
+ if (fastafile == "not found") { mothurOut("fasta is a required parameter for the get.oturep command."); mothurOutEndLine(); abort = true; }
+ else if (fastafile == "not open") { abort = true; }
- fastafile = globaldata->getFastaFile();
- namesfile = globaldata->getNameFile();
- openInputFile(fastafile, in);
+ listfile = validParameter.validFile(parameters, "list", true);
+ if (listfile == "not found") { mothurOut("list is a required parameter for the get.oturep command."); mothurOutEndLine(); abort = true; }
+ else if (listfile == "not open") { abort = true; }
+
+ //check for optional parameter and set defaults
+ // ...at some point should added some additional type checking...
+ line = validParameter.validFile(parameters, "line", false);
+ if (line == "not found") { line = ""; }
+ else {
+ if(line != "all") { splitAtDash(line, lines); allLines = 0; }
+ else { allLines = 1; }
+ }
+
+ label = validParameter.validFile(parameters, "label", false);
+ if (label == "not found") { label = ""; }
+ else {
+ if(label != "all") { splitAtDash(label, labels); allLines = 0; }
+ else { allLines = 1; }
+ }
+
+ //make sure user did not use both the line and label parameters
+ if ((line != "") && (label != "")) { mothurOut("You cannot use both the line and label parameters at the same time. "); mothurOutEndLine(); abort = true; }
+ //if the user has not specified any line or labels use the ones from read.otu
+ else if ((line == "") && (label == "")) {
+ allLines = globaldata->allLines;
+ labels = globaldata->labels;
+ lines = globaldata->lines;
+ }
+
+ namesfile = validParameter.validFile(parameters, "name", true);
+ if (namesfile == "not open") { abort = true; }
+ else if (namesfile == "not found") { namesfile = ""; }
+
+ groupfile = validParameter.validFile(parameters, "group", true);
+ if (groupfile == "not open") { abort = true; }
+ else if (groupfile == "not found") { groupfile = ""; }
+ else {
+ //read in group map info.
+ groupMap = new GroupMap(groupfile);
+ groupMap->readMap();
+ }
- fasta = new FastaMap();
+ if (abort == false) {
+
+ if(globaldata->gSparseMatrix != NULL) {
+ matrix = globaldata->gSparseMatrix;
+ }
+
+ //globaldata->gListVector bin 0 = first name read in distance matrix, globaldata->gListVector bin 1 = second name read in distance matrix
+ if (globaldata->gListVector != NULL) {
+ vector<string> names;
+ string binnames;
+ //map names to rows in sparsematrix
+ for (int i = 0; i < globaldata->gListVector->size(); i++) {
+ names.clear();
+ binnames = globaldata->gListVector->get(i);
+
+ splitAtComma(binnames, names);
+
+ for (int j = 0; j < names.size(); j++) {
+ nameToIndex[names[j]] = i;
+ }
+ }
+ } else { mothurOut("error, no listvector."); mothurOutEndLine(); }
+ // Create a data structure to quickly access the distance information.
+ // It consists of a vector of distance maps, where each map contains
+ // all distances of a certain sequence. Vector and maps are accessed
+ // via the index of a sequence in the distance matrix
+ seqVec = vector<SeqMap>(globaldata->gListVector->size());
+ for (MatData currentCell = matrix->begin(); currentCell != matrix->end(); currentCell++) {
+ seqVec[currentCell->row][currentCell->column] = currentCell->dist;
+ }
+
+ fasta = new FastaMap();
+ }
+ }
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the GetOTURepCommand class Function GetOTURepCommand. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ errorOut(e, "GetOTURepCommand", "GetOTURepCommand");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the GetOTURepCommand class function GetOTURepCommand. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+
+//**********************************************************************************************************************
+
+void GetOTURepCommand::help(){
+ try {
+ mothurOut("The get.oturep command can only be executed after a successful read.dist command.\n");
+ mothurOut("The get.oturep command parameters are list, fasta, name, group, line and label. The fasta and list parameters are required, and you may not use line and label at the same time.\n");
+ mothurOut("The line and label allow you to select what distance levels you would like a output files created for, and are separated by dashes.\n");
+ mothurOut("The get.oturep command should be in the following format: get.oturep(fasta=yourFastaFile, list=yourListFile, name=yourNamesFile, group=yourGroupFile, line=yourLines, label=yourLabels).\n");
+ mothurOut("Example get.oturep(fasta=amazon.fasta, list=amazon.fn.list, group=amazon.groups, line=1-3-5, name=amazon.names).\n");
+ mothurOut("The default value for line and label are all lines in your inputfile.\n");
+ mothurOut("The get.oturep command outputs a .fastarep file for each distance you specify, selecting one OTU representative for each bin.\n");
+ mothurOut("If you provide a groupfile, then it also appends the names of the groups present in that bin.\n");
+ mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
+ }
+ catch(exception& e) {
+ errorOut(e, "GetOTURepCommand", "help");
exit(1);
}
}
+
//**********************************************************************************************************************
GetOTURepCommand::~GetOTURepCommand(){
- delete matrix;
- delete list;
- delete input;
- delete read;
- delete fasta;
+ if (abort == false) {
+ delete input; globaldata->ginput = NULL;
+ delete read;
+ delete fasta;
+ if (groupfile != "") {
+ delete groupMap; globaldata->gGroupmap = NULL;
+ }
+ }
}
//**********************************************************************************************************************
int GetOTURepCommand::execute(){
try {
+
+ if (abort == true) { return 0; }
+
int count = 1;
- string nameRep, name, sequence;
+ int error;
//read fastafile
- fasta->readFastaFile(in);
+ fasta->readFastaFile(fastafile);
+
+ in.close();
//set format to list so input can get listvector
globaldata->setFormat("list");
}
//read list file
- read = new ReadPhilFile(globaldata->getListFile());
+ read = new ReadOTUFile(listfile);
read->read(&*globaldata);
input = globaldata->ginput;
list = globaldata->gListVector;
+ string lastLabel = list->getLabel();
- while(list != NULL){
-
- if(globaldata->allLines == 1 || globaldata->lines.count(count) == 1 || globaldata->labels.count(list->getLabel()) == 1){
-
- //create output file
- string outputFileName = getRootName(globaldata->getListFile()) + list->getLabel() + ".rep.fasta";
- openOutputFile(outputFileName, out);
+ //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+ set<int> userLines = lines;
- cout << list->getLabel() << '\t' << count << endl;
-
- //for each bin in the list vector
- for (int i = 0; i < list->size(); i++) {
- nameRep = FindRep(i);
+
+ while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0) || (userLines.size() != 0))) {
+
+ if (allLines == 1 || lines.count(count) == 1 || labels.count(list->getLabel()) == 1){
+ mothurOut(list->getLabel() + "\t" + toString(list->size())); mothurOutEndLine();
+ error = process(list);
+ if (error == 1) { return 0; } //there is an error in hte input files, abort command
- //print out name and sequence for that bin
- sequence = fasta->getSequence(nameRep);
-
- if (sequence != "not found") {
- nameRep = nameRep + "|" + toString(i+1);
- out << ">" << nameRep << endl;
- out << sequence << endl;
- }else {
- cout << nameRep << " is missing from your fasta or name file. Please correct. " << endl;
- remove(outputFileName.c_str());
- return 0;
- }
- }
-
- out.close();
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
+ userLines.erase(count);
+ }
+
+ if ((anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ delete list;
+ list = input->getListVector(lastLabel);
+ mothurOut(list->getLabel() + "\t" + toString(list->size())); mothurOutEndLine();
+ error = process(list);
+ if (error == 1) { return 0; } //there is an error in hte input files, abort command
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
}
+ lastLabel = list->getLabel();
+
+ delete list;
list = input->getListVector();
count++;
}
-
+ //output error messages about any remaining user labels
+ bool needToRun = false;
+ for (set<string>::iterator it = userLabels.begin(); it != userLabels.end(); it++) {
+ mothurOut("Your file does not include the label " + *it);
+ if (processedLabels.count(list->getLabel()) != 1) {
+ mothurOut(". I will use " + lastLabel + "."); mothurOutEndLine();
+ needToRun = true;
+ }else {
+ mothurOut(". Please refer to " + lastLabel + "."); mothurOutEndLine();
+ }
+ }
+
+ //run last line if you need to
+ if (needToRun == true) {
+ if (list != NULL) { delete list; }
+ list = input->getListVector(lastLabel);
+ mothurOut(list->getLabel() + "\t" + toString(list->size())); mothurOutEndLine();
+ error = process(list);
+ delete list;
+ if (error == 1) { return 0; } //there is an error in hte input files, abort command
+ }
+
+ delete matrix;
+ globaldata->gSparseMatrix = NULL;
+ delete list;
+ globaldata->gListVector = NULL;
+
return 0;
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the GetOTURepCommand class Function execute. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ errorOut(e, "GetOTURepCommand", "execute");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the GetOTURepCommand class function execute. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
- exit(1);
- }
-
}
//**********************************************************************************************************************
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the GetOTURepCommand class Function readNamesFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ errorOut(e, "GetOTURepCommand", "readNamesFile");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the GetOTURepCommand class function readNamesFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
- exit(1);
- }
}
//**********************************************************************************************************************
-string GetOTURepCommand::FindRep(int bin) {
+string GetOTURepCommand::findRep(int bin, string& group, ListVector* thisList, int& binsize) {
try{
vector<string> names;
- map<string, float> sums;
- map<string, float>::iterator it4;
- map<int, string> binMap; //subset of namesToIndex - just member of this bin
- string binnames;
- float min = 10000;
- string minName;
-
- binnames = list->get(bin);
-
+ map<string, string> groups;
+ map<string, string>::iterator groupIt;
+
//parse names into vector
+ string binnames = thisList->get(bin);
splitAtComma(binnames, names);
-
- //if only 1 sequence in bin then that's the rep
- if (names.size() == 1) { return names[0]; }
- else {
- //fill binMap
- for (int i = 0; i < names.size(); i++) {
- for (it3 = nameToIndex.begin(); it3 != nameToIndex.end(); it3++) {
- if (it3->first == names[i]) {
- binMap[it3->second] = it3->first;
-
- //initialize sums map
- sums[it3->first] = 0.0;
- break;
- }
+ binsize = names.size();
+
+ //if you have a groupfile
+ if (groupfile != "") {
+ //find the groups that are in this bin
+ for (size_t i = 0; i < names.size(); i++) {
+ string groupName = groupMap->getGroup(names[i]);
+ if (groupName == "not found") {
+ mothurOut(names[i] + " is missing from your group file. Please correct. "); mothurOutEndLine();
+ groupError = true;
+ } else {
+ groups[groupName] = groupName;
}
}
- //go through each cell in the sparsematrix
- for(MatData currentCell = matrix->begin(); currentCell != matrix->end(); currentCell++){
- //is this a distance between 2 members of this bin
- it = binMap.find(currentCell->row);
- it2 = binMap.find(currentCell->column);
-
- //sum the distance of the sequences in the bin to eachother
- if ((it != binMap.end()) && (it2 != binMap.end())) {
- //this is a cell that repesents the distance between to of this bins members
- sums[it->second] += currentCell->dist;
- sums[it2->second] += currentCell->dist;
- }
+ //turn the groups into a string
+ for (groupIt = groups.begin(); groupIt != groups.end(); groupIt++) {
+ group += groupIt->first + "-";
+ }
+ //rip off last dash
+ group = group.substr(0, group.length()-1);
+ }
+
+ // if only 1 sequence in bin or processing the "unique" line, then
+ // the first sequence of the OTU is the representative one
+ if ((names.size() == 1) || (list->getLabel() == "unique")) {
+ return names[0];
+ } else {
+ vector<int> seqIndex(names.size());
+ vector<float> max_dist(names.size());
+
+ //fill seqIndex and initialize sums
+ for (size_t i = 0; i < names.size(); i++) {
+ seqIndex[i] = nameToIndex[names[i]];
+ max_dist[i] = 0.0;
}
- //smallest sum is the representative
- for (it4 = sums.begin(); it4 != sums.end(); it4++) {
- if (it4->second < min) {
- min = it4->second;
- minName = it4->first;
+ // loop through all entries in seqIndex
+ SeqMap::iterator it;
+ SeqMap currMap;
+ for (size_t i=0; i < seqIndex.size(); i++) {
+ currMap = seqVec[seqIndex[i]];
+ for (size_t j=0; j < seqIndex.size(); j++) {
+ it = currMap.find(seqIndex[j]);
+ if (it != currMap.end()) {
+ max_dist[i] = max(max_dist[i], it->second);
+ max_dist[j] = max(max_dist[j], it->second);
+ }
}
-
}
- return minName;
+ // sequence with the smallest maximum distance is the representative
+ float min = 10000;
+ int minIndex;
+ for (size_t i=0; i < max_dist.size(); i++) {
+ if (max_dist[i] < min) {
+ min = max_dist[i];
+ minIndex = i;
+ }
+ }
+
+ return(names[minIndex]);
}
-
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the GetOTURepCommand class Function FindRep. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ errorOut(e, "GetOTURepCommand", "FindRep");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the GetOTURepCommand class function FindRep. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
- exit(1);
- }
}
+//**********************************************************************************************************************
+int GetOTURepCommand::process(ListVector* processList) {
+ try{
+ string nameRep, name, sequence;
+
+ //create output file
+ string outputFileName = getRootName(listfile) + processList->getLabel() + ".rep.fasta";
+ openOutputFile(outputFileName, out);
+ //for each bin in the list vector
+ for (int i = 0; i < processList->size(); i++) {
+ string groups;
+ int binsize;
+ nameRep = findRep(i, groups, processList, binsize);
+ //print out name and sequence for that bin
+ sequence = fasta->getSequence(nameRep);
+ if (sequence != "not found") {
+ nameRep = nameRep + "|" + toString(i+1);
+ nameRep = nameRep + "|" + toString(binsize);
+ if (groupfile != "") {
+ nameRep = nameRep + "|" + groups;
+ }
+ out << ">" << nameRep << endl;
+ out << sequence << endl;
+ } else {
+ mothurOut(nameRep + " is missing from your fasta or name file. Please correct. "); mothurOutEndLine();
+ remove(outputFileName.c_str());
+ return 1;
+ }
+ }
+
+ out.close();
+ return 0;
+
+ }
+ catch(exception& e) {
+ errorOut(e, "GetOTURepCommand", "process");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
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