#include "dist.h"
#include "eachgapdist.h"
#include "ignoregaps.h"
-
+#include "eachgapdist.h"
//***************************************************************************************************************
void DeCalculator::setMask(string ms) {
}
//***************************************************************************************************************
//gets closest matches to each end, since chimeras will most likely have different parents on each end
-vector<Sequence*> DeCalculator::findClosest(Sequence* querySeq, vector<Sequence*> db, int& numWanted, vector<int>& indexes) {
+vector<Sequence*> DeCalculator::findClosest(Sequence* querySeq, vector<Sequence*>& thisTemplate, vector<Sequence*>& thisFilteredTemplate, int numWanted, int minSim) {
try {
- indexes.clear();
+ //indexes.clear();
vector<Sequence*> seqsMatches;
+
vector<SeqDist> distsLeft;
vector<SeqDist> distsRight;
Sequence queryRight(querySeq->getName(), rightQuery);
//cout << querySeq->getName() << '\t' << leftSpot << '\t' << rightSpot << '\t' << firstBaseSpot << '\t' << lastBaseSpot << endl;
//cout << queryUnAligned.length() << '\t' << queryLeft.getUnaligned().length() << '\t' << queryRight.getUnaligned().length() << endl;
- for(int j = 0; j < db.size(); j++){
+ for(int j = 0; j < thisFilteredTemplate.size(); j++){
- string dbAligned = db[j]->getAligned();
+ string dbAligned = thisFilteredTemplate[j]->getAligned();
string leftDB = dbAligned.substr(firstBaseSpot, (leftSpot-firstBaseSpot+1)); //first 1/3 of the sequence
string rightDB = dbAligned.substr(rightSpot, (lastBaseSpot-rightSpot)); //last 1/3 of the sequence
- Sequence dbLeft(db[j]->getName(), leftDB);
- Sequence dbRight(db[j]->getName(), rightDB);
+ Sequence dbLeft(thisFilteredTemplate[j]->getName(), leftDB);
+ Sequence dbRight(thisFilteredTemplate[j]->getName(), rightDB);
distcalculator->calcDist(queryLeft, dbLeft);
float distLeft = distcalculator->getDist();
float distRight = distcalculator->getDist();
SeqDist subjectLeft;
- subjectLeft.seq = db[j];
+ subjectLeft.seq = NULL;
subjectLeft.dist = distLeft;
subjectLeft.index = j;
distsLeft.push_back(subjectLeft);
SeqDist subjectRight;
- subjectRight.seq = db[j];
+ subjectRight.seq = NULL;
subjectRight.dist = distRight;
subjectRight.index = j;
vector<SeqDist> dists;
float lastRight = distsRight[0].dist;
float lastLeft = distsLeft[0].dist;
- int lasti = 0;
- for (int i = 0; i < distsLeft.size(); i++) {
+ //int lasti = 0;
+ for (int i = 0; i < numWanted+1; i++) {
+
+ if (m->control_pressed) { return seqsMatches; }
+
//add left if you havent already
- it = seen.find(distsLeft[i].seq->getName());
+ it = seen.find(thisTemplate[distsLeft[i].index]->getName());
if (it == seen.end()) {
dists.push_back(distsLeft[i]);
- seen[distsLeft[i].seq->getName()] = distsLeft[i].seq->getName();
+ seen[thisTemplate[distsLeft[i].index]->getName()] = thisTemplate[distsLeft[i].index]->getName();
lastLeft = distsLeft[i].dist;
+// cout << "loop-left\t" << db[distsLeft[i].index]->getName() << '\t' << distsLeft[i].dist << endl;
}
//add right if you havent already
- it = seen.find(distsRight[i].seq->getName());
+ it = seen.find(thisTemplate[distsRight[i].index]->getName());
if (it == seen.end()) {
dists.push_back(distsRight[i]);
- seen[distsRight[i].seq->getName()] = distsRight[i].seq->getName();
+ seen[thisTemplate[distsRight[i].index]->getName()] = thisTemplate[distsRight[i].index]->getName();
lastRight = distsRight[i].dist;
+// cout << "loop-right\t" << db[distsRight[i].index]->getName() << '\t' << distsRight[i].dist << endl;
}
- if (dists.size() > numWanted) { lasti = i; break; } //you have enough results
}
- //add in dups
- lasti++;
- int i = lasti;
- while (i < distsLeft.size()) {
- if (distsLeft[i].dist == lastLeft) { dists.push_back(distsLeft[i]); numWanted++; }
- else { break; }
- i++;
+ //are we still above the minimum similarity cutoff
+ if ((lastLeft >= minSim) || (lastRight >= minSim)) {
+ //add in ties from left
+ int i = numWanted;
+ while (i < distsLeft.size()) {
+ if (distsLeft[i].dist == lastLeft) { dists.push_back(distsLeft[i]); }
+ else { break; }
+ i++;
+ }
+
+ //add in ties from right
+ i = numWanted;
+ while (i < distsRight.size()) {
+ if (distsRight[i].dist == lastRight) { dists.push_back(distsRight[i]); }
+ else { break; }
+ i++;
+ }
}
- i = lasti;
- while (i < distsRight.size()) {
- if (distsRight[i].dist == lastRight) { dists.push_back(distsRight[i]); numWanted++; }
- else { break; }
- i++;
- }
- if (numWanted > dists.size()) { m->mothurOut("numwanted is larger than the number of template sequences, adjusting numwanted."); m->mothurOutEndLine(); numWanted = dists.size(); }
-//cout << numWanted << endl;
- for (int i = 0; i < numWanted; i++) {
-//cout << dists[i].seq->getName() << '\t' << dists[i].dist << endl;
- Sequence* temp = new Sequence(dists[i].seq->getName(), dists[i].seq->getAligned()); //have to make a copy so you can trim and filter without stepping on eachother.
- seqsMatches.push_back(temp);
- indexes.push_back(dists[i].index);
+ //cout << numWanted << endl;
+ for (int i = 0; i < dists.size(); i++) {
+// cout << db[dists[i].index]->getName() << '\t' << dists[i].dist << endl;
+
+ if ((thisTemplate[dists[i].index]->getName() != querySeq->getName()) && (dists[i].dist >= minSim)) {
+ Sequence* temp = new Sequence(thisTemplate[dists[i].index]->getName(), thisTemplate[dists[i].index]->getAligned()); //have to make a copy so you can trim and filter without stepping on eachother.
+
+ seqsMatches.push_back(temp);
+ }
+
}
return seqsMatches;
map<int, int> trimmedPos;
//check to make sure that is not whole seq
if ((rearPos - frontPos - 1) <= 0) {
- m->mothurOut("[ERROR]: when I trim your sequences, the entire sequence is trimmed."); m->mothurOutEndLine();
query->setAligned("");
//trim topMatches
for (int i = 0; i < topMatches.size(); i++) {
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