]> git.donarmstrong.com Git - mothur.git/blobdiff - corraxescommand.cpp
*** empty log message ***
[mothur.git] / corraxescommand.cpp
index 322b7f9d067fc3c9bd1bd8724c8ad43d7b4c3135..db2e9891738cdf57b78dffe1a9c232f18e5de31c 100644 (file)
@@ -8,11 +8,17 @@
  */
 
 #include "corraxescommand.h"
+#include "sharedutilities.h"
 
+//********************************************************************************************************************
+//sorts highes to lowest
+inline bool compareSpearman(spearmanRank left, spearmanRank right){
+       return (left.score > right.score);      
+} 
 //**********************************************************************************************************************
 vector<string> CorrAxesCommand::getValidParameters(){  
        try {
-               string Array[] =  {"axes","shared","relabund","numaxes","label","groups","method","metastats","outputdir","inputdir"};
+               string Array[] =  {"axes","shared","relabund","numaxes","label","groups","method","metadata","outputdir","inputdir"};
                vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
                return myArray;
        }
@@ -69,7 +75,7 @@ CorrAxesCommand::CorrAxesCommand(string option)  {
                
                else {
                        //valid paramters for this command
-                       string Array[] =  {"axes","shared","relabund","numaxes","label","groups","method","metastats","outputdir","inputdir"};
+                       string Array[] =  {"axes","shared","relabund","numaxes","label","groups","method","metadata","outputdir","inputdir"};
                        vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
                        
                        OptionParser parser(option);
@@ -114,6 +120,14 @@ CorrAxesCommand::CorrAxesCommand(string option)  {
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["relabund"] = inputDir + it->second;         }
                                }
+                               
+                               it = parameters.find("metadata");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["metadata"] = inputDir + it->second;         }
+                               }
                        }
                        
                        
@@ -132,6 +146,11 @@ CorrAxesCommand::CorrAxesCommand(string option)  {
                        else if (relabundfile == "not found") { relabundfile = ""; }
                        else { inputFileName = relabundfile; }
                        
+                       metadatafile = validParameter.validFile(parameters, "metadata", true);
+                       if (metadatafile == "not open") { abort = true; }
+                       else if (metadatafile == "not found") { metadatafile = ""; }
+                       else { inputFileName = metadatafile; }
+                       
                        groups = validParameter.validFile(parameters, "groups", false);                 
                        if (groups == "not found") { groups = "";  pickedGroups = false;  }
                        else { 
@@ -145,10 +164,13 @@ CorrAxesCommand::CorrAxesCommand(string option)  {
                        label = validParameter.validFile(parameters, "label", false);                   
                        if (label == "not found") { label = ""; m->mothurOut("You did not provide a label, I will use the first label in your inputfile."); m->mothurOutEndLine(); label=""; }  
                        
-                       if ((relabundfile == "") && (sharedfile == "")) { m->mothurOut("You must provide either a shared or relabund file."); m->mothurOutEndLine(); abort = true;  }
-                       
-                       if ((relabundfile != "") && (sharedfile != "")) { m->mothurOut("You may not use both a shared and relabund file."); m->mothurOutEndLine(); abort = true;  }
+                       if ((relabundfile == "") && (sharedfile == "") && (metadatafile == "")) { m->mothurOut("You must provide either a shared, relabund, or metadata file."); m->mothurOutEndLine(); abort = true;  }
                        
+                       if (metadatafile != "") {
+                               if ((relabundfile != "") || (sharedfile != "")) { m->mothurOut("You may only use one of the following : shared, relabund or metadata file."); m->mothurOutEndLine(); abort = true;  }
+                       }else {
+                               if ((relabundfile != "") && (sharedfile != "")) { m->mothurOut("You may only use one of the following : shared, relabund or metadata file."); m->mothurOutEndLine(); abort = true;  }
+                       }
                        string temp;
                        temp = validParameter.validFile(parameters, "numaxes", false);          if (temp == "not found"){       temp = "3";                             }
                        convert(temp, numaxes); 
@@ -168,7 +190,16 @@ CorrAxesCommand::CorrAxesCommand(string option)  {
 
 void CorrAxesCommand::help(){
        try {
-
+               m->mothurOut("The corr.axes command reads a shared, relabund or metadata file as well as an axes file and calculates the correlation coefficient.\n");
+               m->mothurOut("The corr.axes command parameters are shared, relabund, axes, metadata, groups, method, numaxes and label.  The shared, relabund or metadata and axes parameters are required.  If shared is given the relative abundance is calculated.\n");
+               m->mothurOut("The groups parameter allows you to specify which of the groups you would like included. The group names are separated by dashes.\n");
+               m->mothurOut("The label parameter allows you to select what distance level you would like used, if none is given the first distance is used.\n");
+               m->mothurOut("The method parameter allows you to select what method you would like to use. Options are pearson, spearman and kendall. Default=pearson.\n");
+               m->mothurOut("The numaxes parameter allows you to select the number of axes you would like to use. Default=3.\n");
+               m->mothurOut("The corr.axes command should be in the following format: corr.axes(axes=yourPcoaFile, shared=yourSharedFile, method=yourMethod).\n");
+               m->mothurOut("Example corr.axes(axes=genus.pool.thetayc.genus.lt.pcoa, shared=genus.pool.shared, method=kendall).\n");
+               m->mothurOut("The corr.axes command outputs a .corr.axes file.\n");
+               m->mothurOut("Note: No spaces between parameter labels (i.e. groups), '=' and parameters (i.e.yourGroups).\n\n");
        }
        catch(exception& e) {
                m->errorOut(e, "CorrAxesCommand", "help");      
@@ -191,21 +222,29 @@ int CorrAxesCommand::execute(){
                // use smart distancing to get right sharedRabund and convert to relabund if needed  //
                /************************************************************************************/
                if (sharedfile != "") {  
-                       getShared(); 
-                       if (m->control_pressed) {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } return 0; }
-                       if (lookup[0] == NULL) { m->mothurOut("[ERROR] reading shared file."); m->mothurOutEndLine(); return 0; }
+                       InputData* input = new InputData(sharedfile, "sharedfile");
+                       getSharedFloat(input); 
+                       delete input;
                        
-                       //fills lookupFloat with relative abundance values from lookup
-                       convertToRelabund();
+                       if (m->control_pressed) {  for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
+                       if (lookupFloat[0] == NULL) { m->mothurOut("[ERROR] reading relabund file."); m->mothurOutEndLine(); return 0; }
+                       
+               }else if (relabundfile != "") { 
+                       InputData* input = new InputData(relabundfile, "relabund");
+                       getSharedFloat(input); 
+                       delete input;
                        
-                       for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } 
-               }else { 
-                       getSharedFloat(); 
                        if (m->control_pressed) {  for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
                        if (lookupFloat[0] == NULL) { m->mothurOut("[ERROR] reading relabund file."); m->mothurOutEndLine(); return 0; }
                        
+               }else if (metadatafile != "") { 
+                       getMetadata();  //reads metadata file and store in lookupFloat, saves column headings in metadataLabels for later
+                       if (m->control_pressed) {  for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
+                       if (lookupFloat[0] == NULL) { m->mothurOut("[ERROR] reading metadata file."); m->mothurOutEndLine(); return 0; }
+                       
                        if (pickedGroups) { eliminateZeroOTUS(lookupFloat); }
-               }
+                       
+               }else { m->mothurOut("[ERROR]: no file given."); m->mothurOutEndLine(); return 0; }
                
                if (m->control_pressed) {  for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
                
@@ -233,15 +272,27 @@ int CorrAxesCommand::execute(){
                
                if (m->control_pressed) {  for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
                
-               string outputFileName = outputDir + m->getRootName(m->getSimpleName(inputFileName)) + "corr.axes";
+               /*************************************************************************************/
+               // calc the r values                                                                                                                            //
+               /************************************************************************************/
+               
+               string outputFileName = outputDir + m->getRootName(m->getSimpleName(inputFileName)) + method + ".corr.axes";
                outputNames.push_back(outputFileName); outputTypes["corr.axes"].push_back(outputFileName);      
                ofstream out;
                m->openOutputFile(outputFileName, out);
+               out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
+               
+               //output headings
+               if (metadatafile == "") {  out << "OTU";        }
+               else {  out << "Feature";                                               }
+
+               for (int i = 0; i < numaxes; i++) { out << '\t' << "axis" << (i+1); }
+               out << "\tlength" << endl;
                
                if (method == "pearson")                {  calcPearson(axes, out);      }
-               //else if (method == "spearman")        {  calcSpearman(axes, out); }
-               //else if (method == "kendall") {  calcKendal(axes, out);       }
-               //else { m->mothurOut("[ERROR]: Invalid method."); m->mothurOutEndLine(); }
+               else if (method == "spearman")  {  calcSpearman(axes, out); }
+               else if (method == "kendall")   {  calcKendall(axes, out);      }
+               else { m->mothurOut("[ERROR]: Invalid method."); m->mothurOutEndLine(); }
                
                out.close();
                for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  }
@@ -278,6 +329,9 @@ int CorrAxesCommand::calcPearson(map<string, vector<float> >& axes, ofstream& ou
           //for each otu
           for (int i = 0; i < lookupFloat[0]->getNumBins(); i++) {
                   
+                  if (metadatafile == "") {  out << i+1;       }
+                  else {  out << metadataLabels[i];            }
+                                  
                   //find the averages this otu - Y
                   float sumOtu = 0.0;
                   for (int j = 0; j < lookupFloat.size(); j++) {
@@ -285,7 +339,36 @@ int CorrAxesCommand::calcPearson(map<string, vector<float> >& axes, ofstream& ou
                   }
                   float Ybar = sumOtu / (float) lookupFloat.size();
                   
-                  //find the average
+                  vector<float> rValues(averageAxes.size());
+
+                  //find r value for each axis
+                  for (int k = 0; k < averageAxes.size(); k++) {
+                          
+                          double r = 0.0;
+                          double numerator = 0.0;
+                          double denomTerm1 = 0.0;
+                          double denomTerm2 = 0.0;
+                          for (int j = 0; j < lookupFloat.size(); j++) {
+                                  float Yi = lookupFloat[j]->getAbundance(i);
+                                  float Xi = axes[lookupFloat[j]->getGroup()][k];
+                                  
+                                  numerator += ((Xi - averageAxes[k]) * (Yi - Ybar));
+                                  denomTerm1 += ((Xi - averageAxes[k]) * (Xi - averageAxes[k]));
+                                  denomTerm2 += ((Yi - Ybar) * (Yi - Ybar));
+                          }
+                          
+                          double denom = (sqrt(denomTerm1) * sqrt(denomTerm2));
+                          
+                          r = numerator / denom;
+                          rValues[k] = r;
+                          out << '\t' << r; 
+                  }
+                  
+                  double sum = 0;
+                  for(int k=0;k<rValues.size();k++){
+                          sum += rValues[k] * rValues[k];
+                  }
+                  out << '\t' << sqrt(sum) << endl;
           }
                   
           return 0;
@@ -296,89 +379,263 @@ int CorrAxesCommand::calcPearson(map<string, vector<float> >& axes, ofstream& ou
    }
 }
 //**********************************************************************************************************************
-int CorrAxesCommand::getShared(){
+int CorrAxesCommand::calcSpearman(map<string, vector<float> >& axes, ofstream& out) {
        try {
-               InputData* input = new InputData(sharedfile, "sharedfile");
-               lookup = input->getSharedRAbundVectors();
-               string lastLabel = lookup[0]->getLabel();
                
-               if (label == "") { label = lastLabel; delete input; return 0; }
+               //format data
+               vector< vector<spearmanRank> > scores; scores.resize(numaxes);
+               for (map<string, vector<float> >::iterator it = axes.begin(); it != axes.end(); it++) {
+                       vector<float> temp = it->second;
+                       for (int i = 0; i < temp.size(); i++) {
+                               spearmanRank member(it->first, temp[i]);
+                               scores[i].push_back(member);  
+                       }
+               }
                
-               //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
-               set<string> labels; labels.insert(label);
-               set<string> processedLabels;
-               set<string> userLabels = labels;
+               //sort each axis
+               for (int i = 0; i < numaxes; i++) {  sort(scores[i].begin(), scores[i].end(), compareSpearman); }
                
-               //as long as you are not at the end of the file or done wih the lines you want
-               while((lookup[0] != NULL) && (userLabels.size() != 0)) {
-                       if (m->control_pressed) {  delete input; return 0;  }
+               //find ranks of xi in each axis
+               map<string, vector<float> > rankAxes;
+               for (int i = 0; i < numaxes; i++) {
                        
-                       if(labels.count(lookup[0]->getLabel()) == 1){
-                               processedLabels.insert(lookup[0]->getLabel());
-                               userLabels.erase(lookup[0]->getLabel());
-                               break;
+                       vector<spearmanRank> ties;
+                       int rankTotal = 0;
+                       for (int j = 0; j < scores[i].size(); j++) {
+                               rankTotal += j;
+                               ties.push_back(scores[i][j]);
+                               
+                               if (j != scores.size()-1) { // you are not the last so you can look ahead
+                                       if (scores[i][j].score != scores[i][j+1].score) { // you are done with ties, rank them and continue
+                                               for (int k = 0; k < ties.size(); k++) {
+                                                       float thisrank = rankTotal / (float) ties.size();
+                                                       rankAxes[ties[k].name].push_back(thisrank);
+                                               }
+                                               ties.clear();
+                                               rankTotal = 0;
+                                       }
+                               }else { // you are the last one
+                                       for (int k = 0; k < ties.size(); k++) {
+                                               float thisrank = rankTotal / (float) ties.size();
+                                               rankAxes[ties[k].name].push_back(thisrank);
+                                       }
+                               }
+                       }
+               }
+               
+                               
+               
+               //for each otu
+               for (int i = 0; i < lookupFloat[0]->getNumBins(); i++) {
+                       
+                       if (metadatafile == "") {  out << i+1;  }
+                       else {  out << metadataLabels[i];               }
+                       
+                       //find the ranks of this otu - Y
+                       vector<spearmanRank> otuScores;
+                       for (int j = 0; j < lookupFloat.size(); j++) {
+                               spearmanRank member(lookupFloat[j]->getGroup(), lookupFloat[j]->getAbundance(i));
+                               otuScores.push_back(member);
                        }
                        
-                       if ((m->anyLabelsToProcess(lookup[0]->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
-                               string saveLabel = lookup[0]->getLabel();
+                       sort(otuScores.begin(), otuScores.end(), compareSpearman);
+                       
+                       map<string, float> rankOtus;
+                       vector<spearmanRank> ties;
+                       int rankTotal = 0;
+                       for (int j = 0; j < otuScores.size(); j++) {
+                               rankTotal += j;
+                               ties.push_back(otuScores[j]);
                                
-                               for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } 
-                               lookup = input->getSharedRAbundVectors(lastLabel);
+                               if (j != scores.size()-1) { // you are not the last so you can look ahead
+                                       if (otuScores[j].score != otuScores[j+1].score) { // you are done with ties, rank them and continue
+                                               for (int k = 0; k < ties.size(); k++) {
+                                                       float thisrank = rankTotal / (float) ties.size();
+                                                       rankOtus[ties[k].name] = thisrank;
+                                               }
+                                               ties.clear();
+                                               rankTotal = 0;
+                                       }
+                               }else { // you are the last one
+                                       for (int k = 0; k < ties.size(); k++) {
+                                               float thisrank = rankTotal / (float) ties.size();
+                                               rankOtus[ties[k].name] = thisrank;
+                                       }
+                               }
+                       }
+                       
+                       vector<double> pValues(numaxes);
+                       //calc spearman ranks for each axis for this otu
+                       for (int j = 0; j < numaxes; j++) {
                                
-                               processedLabels.insert(lookup[0]->getLabel());
-                               userLabels.erase(lookup[0]->getLabel());
+                               double di = 0.0;
+                               for (int k = 0; k < lookupFloat.size(); k++) {
+                                       
+                                       float xi = rankAxes[lookupFloat[k]->getGroup()][j];
+                                       float yi = rankOtus[lookupFloat[k]->getGroup()];
+                                       
+                                       di += ((xi - yi) * (xi - yi));
+                               }
                                
-                               //restore real lastlabel to save below
-                               lookup[0]->setLabel(saveLabel);
-                               break;
+                               int n = lookupFloat.size();
+                               double p = 1.0 - ((6 * di) / (float) (n * ((n*n) - 1)));
+                               
+                               out  << '\t' << p;
+                               pValues[j] = p;
+
                        }
-                       
-                       lastLabel = lookup[0]->getLabel();                      
-                       
-                       //get next line to process
-                       //prevent memory leak
-                       for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } 
-                       lookup = input->getSharedRAbundVectors();
+
+                       double sum = 0;
+                       for(int k=0;k<numaxes;k++){
+                               sum += pValues[k] * pValues[k];
+                       }
+                       out << '\t' << sqrt(sum) << endl;
                }
                
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "CorrAxesCommand", "calcSpearman");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+int CorrAxesCommand::calcKendall(map<string, vector<float> >& axes, ofstream& out) {
+       try {
                
-               if (m->control_pressed) { delete input; return 0;  }
+               //format data
+               vector< vector<spearmanRank> > scores; scores.resize(numaxes);
+               for (map<string, vector<float> >::iterator it = axes.begin(); it != axes.end(); it++) {
+                       vector<float> temp = it->second;
+                       for (int i = 0; i < temp.size(); i++) {
+                               spearmanRank member(it->first, temp[i]);
+                               scores[i].push_back(member);  
+                       }
+               }
                
-               //output error messages about any remaining user labels
-               set<string>::iterator it;
-               bool needToRun = false;
-               for (it = userLabels.begin(); it != userLabels.end(); it++) {  
-                       m->mothurOut("Your file does not include the label " + *it); 
-                       if (processedLabels.count(lastLabel) != 1) {
-                               m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
-                               needToRun = true;
-                       }else {
-                               m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
+               //sort each axis
+               for (int i = 0; i < numaxes; i++) {  sort(scores[i].begin(), scores[i].end(), compareSpearman); }
+               
+               //convert scores to ranks of xi in each axis
+               for (int i = 0; i < numaxes; i++) {
+                       
+                       vector<spearmanRank*> ties;
+                       int rankTotal = 0;
+                       for (int j = 0; j < scores[i].size(); j++) {
+                               rankTotal += j;
+                               ties.push_back(&(scores[i][j]));
+                               
+                               if (j != scores.size()-1) { // you are not the last so you can look ahead
+                                       if (scores[i][j].score != scores[i][j+1].score) { // you are done with ties, rank them and continue
+                                               for (int k = 0; k < ties.size(); k++) {
+                                                       float thisrank = rankTotal / (float) ties.size();
+                                                       (*ties[k]).score = thisrank;
+                                               }
+                                               ties.clear();
+                                               rankTotal = 0;
+                                       }
+                               }else { // you are the last one
+                                       for (int k = 0; k < ties.size(); k++) {
+                                               float thisrank = rankTotal / (float) ties.size();
+                                               (*ties[k]).score = thisrank;
+                                       }
+                               }
                        }
                }
                
-               //run last label if you need to
-               if (needToRun == true)  {
-                       for (int i = 0; i < lookup.size(); i++) {  if (lookup[i] != NULL) {     delete lookup[i];       } } 
-                       lookup = input->getSharedRAbundVectors(lastLabel);
-               }       
+               //for each otu
+               for (int i = 0; i < lookupFloat[0]->getNumBins(); i++) {
+               
+                       if (metadatafile == "") {  out << i+1;  }
+                       else {  out << metadataLabels[i];               }
+                       
+                       //find the ranks of this otu - Y
+                       vector<spearmanRank> otuScores;
+                       for (int j = 0; j < lookupFloat.size(); j++) {
+                               spearmanRank member(lookupFloat[j]->getGroup(), lookupFloat[j]->getAbundance(i));
+                               otuScores.push_back(member);
+                       }
+                                               
+                       sort(otuScores.begin(), otuScores.end(), compareSpearman);
+                       
+                       map<string, float> rankOtus;
+                       vector<spearmanRank> ties;
+                       int rankTotal = 0;
+                       for (int j = 0; j < otuScores.size(); j++) {
+                               rankTotal += j;
+                               ties.push_back(otuScores[j]);
+                               
+                               if (j != scores.size()-1) { // you are not the last so you can look ahead
+                                       if (otuScores[j].score != otuScores[j+1].score) { // you are done with ties, rank them and continue
+                                               for (int k = 0; k < ties.size(); k++) {
+                                                       float thisrank = rankTotal / (float) ties.size();
+                                                       rankOtus[ties[k].name] = thisrank;
+                                               }
+                                               ties.clear();
+                                               rankTotal = 0;
+                                       }
+                               }else { // you are the last one
+                                       for (int k = 0; k < ties.size(); k++) {
+                                               float thisrank = rankTotal / (float) ties.size();
+                                               rankOtus[ties[k].name] = thisrank;
+                                       }
+                               }
+                       }
+                       vector<double> pValues(numaxes);
+                       
+                       //calc spearman ranks for each axis for this otu
+                       for (int j = 0; j < numaxes; j++) {
+                       
+                               int P = 0;
+                               //assemble otus ranks in same order as axis ranks
+                               vector<spearmanRank> otus; 
+                               for (int l = 0; l < scores[j].size(); l++) {   
+                                       spearmanRank member(scores[j][l].name, rankOtus[scores[j][l].name]);
+                                       otus.push_back(member);
+                               }
+                               
+                               for (int l = 0; l < scores[j].size(); l++) {
+                                       
+                                       int numWithHigherRank = 0;
+                                       float thisrank = otus[l].score;
+                                       
+                                       for (int u = l; u < scores[j].size(); u++) {
+                                               if (otus[u].score > thisrank) { numWithHigherRank++; }
+                                       }
+                                       
+                                       P += numWithHigherRank;
+                               }
+                               
+                               int n = lookupFloat.size();
+                               
+                               double p = ( (4 * P) / (float) (n * (n - 1)) ) - 1.0;
+                               
+                               out << '\t' << p;
+                               pValues[j] = p;
+
+                       }
+                       
+                       double sum = 0;
+                       for(int k=0;k<numaxes;k++){
+                               sum += pValues[k] * pValues[k];
+                       }
+                       out << '\t' << sqrt(sum) << endl;
+               }
                
-               delete input;
                return 0;
        }
        catch(exception& e) {
-               m->errorOut(e, "CorrAxesCommand", "getShared"); 
+               m->errorOut(e, "CorrAxesCommand", "calcKendall");
                exit(1);
        }
 }
 //**********************************************************************************************************************
-int CorrAxesCommand::getSharedFloat(){
+int CorrAxesCommand::getSharedFloat(InputData* input){
        try {
-               InputData* input = new InputData(relabundfile, "relabund");
                lookupFloat = input->getSharedRAbundFloatVectors();
                string lastLabel = lookupFloat[0]->getLabel();
                
-               if (label == "") { label = lastLabel; delete input; return 0; }
+               if (label == "") { label = lastLabel;  return 0; }
                
                //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
                set<string> labels; labels.insert(label);
@@ -388,7 +645,7 @@ int CorrAxesCommand::getSharedFloat(){
                //as long as you are not at the end of the file or done wih the lines you want
                while((lookupFloat[0] != NULL) && (userLabels.size() != 0)) {
                        
-                       if (m->control_pressed) {  delete input; return 0;  }
+                       if (m->control_pressed) {  return 0;  }
                        
                        if(labels.count(lookupFloat[0]->getLabel()) == 1){
                                processedLabels.insert(lookupFloat[0]->getLabel());
@@ -419,7 +676,7 @@ int CorrAxesCommand::getSharedFloat(){
                }
                
                
-               if (m->control_pressed) { delete input; return 0;  }
+               if (m->control_pressed) { return 0;  }
                
                //output error messages about any remaining user labels
                set<string>::iterator it;
@@ -440,7 +697,6 @@ int CorrAxesCommand::getSharedFloat(){
                        lookupFloat = input->getSharedRAbundFloatVectors(lastLabel);
                }       
                
-               delete input;
                return 0;
        }
        catch(exception& e) {
@@ -448,43 +704,6 @@ int CorrAxesCommand::getSharedFloat(){
                exit(1);
        }
 }
-/*****************************************************************/
-int CorrAxesCommand::convertToRelabund(){
-       try {
-               
-               vector<SharedRAbundFloatVector*> newLookup;
-               for (int i = 0; i < lookup.size(); i++) {
-                       SharedRAbundFloatVector* temp = new SharedRAbundFloatVector();
-                       temp->setLabel(lookup[i]->getLabel());
-                       temp->setGroup(lookup[i]->getGroup());
-                       newLookup.push_back(temp);
-               }
-               
-               for (int i = 0; i < lookup.size(); i++) {
-                       
-                       for (int j = 0; j < lookup[i]->getNumBins(); j++) {
-                               
-                               if (m->control_pressed) { return 0; }
-                               
-                               int abund = lookup[i]->getAbundance(j);
-                               
-                               float relabund = abund / (float) lookup[i]->getNumSeqs();
-                               
-                               newLookup[i]->push_back(relabund, lookup[i]->getGroup());
-                       }
-               }
-               
-               if (pickedGroups) { eliminateZeroOTUS(newLookup); }
-               
-               lookupFloat = newLookup;
-               
-               return 0;
-       }
-       catch(exception& e) {
-               m->errorOut(e, "CorrAxesCommand", "convertToRelabund"); 
-               exit(1);
-       }
-}
 //**********************************************************************************************************************
 int CorrAxesCommand::eliminateZeroOTUS(vector<SharedRAbundFloatVector*>& thislookup) {
        try {
@@ -547,7 +766,8 @@ map<string, vector<float> > CorrAxesCommand::readAxes(){
                                headerLine = headerLine.substr(pos+4);
                        }else { done = true; }
                }
-               cout << "count = " << count << endl;    
+               
+               if (numaxes > count) { m->mothurOut("You requested " + toString(numaxes) + " axes, but your file only includes " + toString(count) + ". Using " + toString(count) + "."); m->mothurOutEndLine(); numaxes = count; }
                
                while (!in.eof()) {
                        
@@ -583,7 +803,79 @@ map<string, vector<float> > CorrAxesCommand::readAxes(){
                return axes;
        }
        catch(exception& e) {
-               m->errorOut(e, "CorrAxesCommand", "convertToRelabund"); 
+               m->errorOut(e, "CorrAxesCommand", "readAxes");  
+               exit(1);
+       }
+}
+/*****************************************************************/
+int CorrAxesCommand::getMetadata(){
+       try {
+               vector<string> groupNames;
+               
+               ifstream in;
+               m->openInputFile(axesfile, in);
+               
+               string headerLine = m->getline(in); m->gobble(in);
+               istringstream iss (headerLine,istringstream::in);
+               
+               //read the first label, because it refers to the groups
+               string columnLabel;
+               iss >> columnLabel; m->gobble(iss); 
+               
+               //save names of columns you are reading
+               while (!iss.eof()) {
+                       iss >> columnLabel; m->gobble(iss);
+                       metadataLabels.push_back(columnLabel);
+               }
+               int count = metadataLabels.size();
+               
+               //read rest of file
+               while (!in.eof()) {
+                       
+                       if (m->control_pressed) { in.close(); return 0; }
+                       
+                       string group = "";
+                       in >> group; m->gobble(in);
+                       groupNames.push_back(group);
+                       
+                       SharedRAbundFloatVector* tempLookup = new SharedRAbundFloatVector();
+                       tempLookup->setGroup(group);
+                       tempLookup->setLabel("1");
+                       
+                       for (int i = 0; i < count; i++) {
+                               float temp = 0.0;
+                               in >> temp; 
+                               
+                               tempLookup->push_back(temp, group);
+                       }
+                       
+                       lookupFloat.push_back(tempLookup);
+                       
+                       m->gobble(in);
+               }
+               in.close();
+               
+               //remove any groups the user does not want, and set globaldata->groups with only valid groups
+               SharedUtil* util;
+               util = new SharedUtil();
+               
+               util->setGroups(globaldata->Groups, groupNames);
+               
+               for (int i = 0; i < lookupFloat.size(); i++) {
+                       //if this sharedrabund is not from a group the user wants then delete it.
+                       if (util->isValidGroup(lookupFloat[i]->getGroup(), globaldata->Groups) == false) { 
+                               delete lookupFloat[i]; lookupFloat[i] = NULL;
+                               lookupFloat.erase(lookupFloat.begin()+i); 
+                               i--; 
+                       }
+               }
+               
+               delete util;
+               
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "CorrAxesCommand", "getMetadata");       
                exit(1);
        }
 }