use Getopt::Long;
use Pod::Usage;
-use FindBin;
-use lib $FindBin::Bin;
use File::Basename;
use File::Path 'rmtree';
-use strict;
+use FindBin;
+use lib $FindBin::RealBin;
use rsem_perl_utils;
+use Env qw(@PATH);
+push(@PATH, $FindBin::RealBin, "$FindBin::RealBin/sam");
+
+use strict;
+
my ($in_file, $out_file) = ();
my @tmp_dirs = ();
pod2usage(-msg => "Input file's suffix is neither sam nor bam!", -exitval => 2, -verbose => 2) if (($suf ne "sam") && ($suf ne "bam"));
my $isSam = ($suf eq "sam");
-$dir = "$FindBin::Bin/";
-
my $temp_dir = "$out_file.temp";
if (-d $temp_dir) { print "Warning: $temp_dir exists, convert-sam-for-rsem will write temporary files into this folder and delete it after it finishes!\n"; }
else {
if (!$isSam) {
$sam_file = "$temp_dir/input.sam";
- $command = $dir."sam/samtools view -h -o $sam_file $in_file";
+ $command = "samtools view -h -o $sam_file $in_file";
&runCommand($command);
}
else {
# Phase II, parse the temporary SAM file to make paired-end alignments' two mates adjacent to each other
-$command = $dir."rsem-scan-for-paired-end-reads $tmp_sam $out_file";
+$command = "rsem-scan-for-paired-end-reads $tmp_sam $out_file";
&runCommand($command);
# delete temporary directory
# Phase III, validate if the resulting bam file is correct
-$command = $dir."rsem-sam-validator $out_file";
+$command = "rsem-sam-validator $out_file";
&runCommand($command);
__END__