]> git.donarmstrong.com Git - mothur.git/blobdiff - chimeraslayer.cpp
tie breaker fix to chimera.slayer
[mothur.git] / chimeraslayer.cpp
index 78c9f9e9e24d0138b158791d7d3bce2f39eca026..77a8695a579d64b250cf98aa3852cf4975e7f90f 100644 (file)
@@ -13,7 +13,7 @@
 #include "blastdb.hpp"
 
 //***************************************************************************************************************
-ChimeraSlayer::ChimeraSlayer(string file, string temp, string mode, int k, int ms, int mms, int win, float div, 
+ChimeraSlayer::ChimeraSlayer(string file, string temp, bool trim, string mode, int k, int ms, int mms, int win, float div, 
 int minsim, int mincov, int minbs, int minsnp, int par, int it, int inc, int numw, bool r) : Chimera()  {      
        try {
                fastafile = file;
@@ -33,6 +33,7 @@ int minsim, int mincov, int minbs, int minsnp, int par, int it, int inc, int num
                increment = inc;
                numWanted = numw;
                realign = r; 
+               trimChimera = trim;
        
                decalc = new DeCalculator();    
                
@@ -44,7 +45,7 @@ int minsim, int mincov, int minbs, int minsnp, int par, int it, int inc, int num
        }
 }
 //***************************************************************************************************************
-ChimeraSlayer::ChimeraSlayer(string file, string temp, string name, string mode, string abunds, int k, int ms, int mms, int win, float div, 
+ChimeraSlayer::ChimeraSlayer(string file, string temp, bool trim, string name, string mode, string abunds, int k, int ms, int mms, int win, float div, 
                                                         int minsim, int mincov, int minbs, int minsnp, int par, int it, int inc, int numw, bool r) : Chimera()  {      
        try {
                fastafile = file; templateSeqs = readSeqs(fastafile);
@@ -65,6 +66,7 @@ ChimeraSlayer::ChimeraSlayer(string file, string temp, string name, string mode,
                numWanted = numw;
                realign = r; 
                includeAbunds = abunds;
+               trimChimera = trim;
                
                //read name file and create nameMapRank
                readNameFile(name);
@@ -256,7 +258,8 @@ int ChimeraSlayer::doPrep() {
                }else if (searchMethod == "blast") {
                
                        //generate blastdb
-                       databaseLeft = new BlastDB(-2.0, -1.0, match, misMatch);
+                       databaseLeft = new BlastDB(-1.0, -1.0, 1, -3);
+
                        for (int i = 0; i < templateSeqs.size(); i++) {         databaseLeft->addSequence(*templateSeqs[i]);    }
                        databaseLeft->generateDB();
                        databaseLeft->setNumSeqs(templateSeqs.size());
@@ -382,7 +385,8 @@ vector<Sequence*> ChimeraSlayer::getTemplate(Sequence* q) {
                }else if (searchMethod == "blast") {
                        
                        //generate blastdb
-                       databaseLeft = new BlastDB(-2.0, -1.0, match, misMatch);
+                       databaseLeft = new BlastDB(-1.0, -1.0, 1, -3);
+
                        for (int i = 0; i < thisTemplate.size(); i++) { if (m->control_pressed) { return thisTemplate; }  databaseLeft->addSequence(*thisTemplate[i]);  }
                        databaseLeft->generateDB();
                        databaseLeft->setNumSeqs(thisTemplate.size());
@@ -414,8 +418,11 @@ void ChimeraSlayer::printHeader(ostream& out) {
        out << "Name\tLeftParent\tRightParent\tDivQLAQRB\tPerIDQLAQRB\tBootStrapA\tDivQLBQRA\tPerIDQLBQRA\tBootStrapB\tFlag\tLeftWindow\tRightWindow\n";
 }
 //***************************************************************************************************************
-int ChimeraSlayer::print(ostream& out, ostream& outAcc) {
+Sequence* ChimeraSlayer::print(ostream& out, ostream& outAcc) {
        try {
+               Sequence* trim = NULL;
+               if (trimChimera) { trim = trimQuery; }
+               
                if (chimeraFlags == "yes") {
                        string chimeraFlag = "no";
                        if(  (chimeraResults[0].bsa >= minBS && chimeraResults[0].divr_qla_qrb >= divR)
@@ -427,6 +434,21 @@ int ChimeraSlayer::print(ostream& out, ostream& outAcc) {
                                if ((chimeraResults[0].bsa >= minBS) || (chimeraResults[0].bsb >= minBS)) {
                                        m->mothurOut(querySeq->getName() + "\tyes"); m->mothurOutEndLine();
                                        outAcc << querySeq->getName() << endl;
+                                       
+                                       if (trimChimera) {  
+                                               int lengthLeft = spotMap[chimeraResults[0].winLEnd] - spotMap[chimeraResults[0].winLStart];
+                                               int lengthRight = spotMap[chimeraResults[0].winREnd] - spotMap[chimeraResults[0].winRStart];
+                                               
+                                               string newAligned = trim->getAligned();
+
+                                               if (lengthLeft > lengthRight) { //trim right
+                                                       for (int i = (spotMap[chimeraResults[0].winRStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                               }else { //trim left
+                                                       for (int i = 0; i < spotMap[chimeraResults[0].winLEnd]; i++) { newAligned[i] = '.'; }
+                                               }
+                                               trim->setAligned(newAligned);
+                                       }
+                                               
                                }
                        }
                        
@@ -434,7 +456,7 @@ int ChimeraSlayer::print(ostream& out, ostream& outAcc) {
                        out << endl;
                }else {  out << querySeq->getName() << "\tno" << endl;  }
                
-               return 0;
+               return trim;
                
        }
        catch(exception& e) {
@@ -442,15 +464,251 @@ int ChimeraSlayer::print(ostream& out, ostream& outAcc) {
                exit(1);
        }
 }
+//***************************************************************************************************************
+Sequence* ChimeraSlayer::print(ostream& out, ostream& outAcc, data_results leftPiece, data_results rightPiece) {
+       try {
+               Sequence* trim = NULL;
+                               
+               if (trimChimera) { 
+                       string aligned = leftPiece.trimQuery.getAligned() + rightPiece.trimQuery.getAligned();
+                       trim = new Sequence(leftPiece.trimQuery.getName(), aligned); 
+               }
+               
+               if ((leftPiece.flag == "yes") || (rightPiece.flag == "yes")) {
+                       
+                       string chimeraFlag = "no";
+                       if (leftPiece.flag == "yes") {
+                               
+                               if(  (leftPiece.results[0].bsa >= minBS && leftPiece.results[0].divr_qla_qrb >= divR)
+                                       ||
+                                       (leftPiece.results[0].bsb >= minBS && leftPiece.results[0].divr_qlb_qra >= divR) ) { chimeraFlag = "yes"; }
+                       }
+                       
+                       if (rightPiece.flag == "yes") {
+                               if ( (rightPiece.results[0].bsa >= minBS && rightPiece.results[0].divr_qla_qrb >= divR)
+                                ||
+                                (rightPiece.results[0].bsb >= minBS && rightPiece.results[0].divr_qlb_qra >= divR) ) { chimeraFlag = "yes"; }
+                       }
+                       
+                       bool rightChimeric = false;
+                       bool leftChimeric = false;
+                       
+                       if (chimeraFlag == "yes") {     
+                               //which peice is chimeric or are both
+                               if (rightPiece.flag == "yes") { if ((rightPiece.results[0].bsa >= minBS) || (rightPiece.results[0].bsb >= minBS)) { rightChimeric = true; } }
+                               if (leftPiece.flag == "yes") { if ((leftPiece.results[0].bsa >= minBS) || (leftPiece.results[0].bsb >= minBS))  { leftChimeric = true;  } }
+                               
+                               if (rightChimeric || leftChimeric) {
+                                       m->mothurOut(querySeq->getName() + "\tyes"); m->mothurOutEndLine();
+                                       outAcc << querySeq->getName() << endl;
+                                       
+                                       if (trimChimera) {  
+                                               string newAligned = trim->getAligned();
+                                                                                               
+                                               //right side is fine so keep that
+                                               if ((leftChimeric) && (!rightChimeric)) {
+                                                       for (int i = 0; i < leftPiece.spotMap[leftPiece.results[0].winREnd]; i++) { newAligned[i] = '.'; } 
+                                               }else if ((!leftChimeric) && (rightChimeric)) { //leftside is fine so keep that
+                                                       for (int i = (rightPiece.spotMap[rightPiece.results[0].winLStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                               }else { //both sides are chimeric, keep longest piece
+                                                       
+                                                       int lengthLeftLeft = leftPiece.spotMap[leftPiece.results[0].winLEnd] - leftPiece.spotMap[leftPiece.results[0].winLStart];
+                                                       int lengthLeftRight = leftPiece.spotMap[leftPiece.results[0].winREnd] - leftPiece.spotMap[leftPiece.results[0].winRStart];
+                                                       
+                                                       int longest = 1; // leftleft = 1, leftright = 2, rightleft = 3 rightright = 4
+                                                       int length = lengthLeftLeft;
+                                                       if (lengthLeftLeft < lengthLeftRight) { longest = 2;  length = lengthLeftRight; }
+                                                       
+                                                       int lengthRightLeft = rightPiece.spotMap[rightPiece.results[0].winLEnd] - rightPiece.spotMap[rightPiece.results[0].winLStart];
+                                                       int lengthRightRight = rightPiece.spotMap[rightPiece.results[0].winREnd] - rightPiece.spotMap[rightPiece.results[0].winRStart];
+                                                       
+                                                       if (lengthRightLeft > length) { longest = 3; length = lengthRightLeft;  }
+                                                       if (lengthRightRight > length) { longest = 4; }
+                                                       
+                                                       if (longest == 1) { //leftleft
+                                                               for (int i = (leftPiece.spotMap[leftPiece.results[0].winRStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                                       }else if (longest == 2) { //leftright
+                                                               //get rid of leftleft
+                                                               for (int i = (leftPiece.spotMap[leftPiece.results[0].winLStart]-1); i < (leftPiece.spotMap[leftPiece.results[0].winLEnd]-1); i++) { newAligned[i] = '.'; }
+                                                               //get rid of right
+                                                               for (int i = (rightPiece.spotMap[rightPiece.results[0].winLStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                                       }else if (longest == 3) { //rightleft
+                                                               //get rid of left
+                                                               for (int i = 0; i < leftPiece.spotMap[leftPiece.results[0].winREnd]; i++) { newAligned[i] = '.'; } 
+                                                               //get rid of rightright
+                                                               for (int i = (rightPiece.spotMap[rightPiece.results[0].winRStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                                       }else { //rightright
+                                                               //get rid of left
+                                                               for (int i = 0; i < leftPiece.spotMap[leftPiece.results[0].winREnd]; i++) { newAligned[i] = '.'; } 
+                                                               //get rid of rightleft
+                                                               for (int i = (rightPiece.spotMap[rightPiece.results[0].winLStart]-1); i < (rightPiece.spotMap[rightPiece.results[0].winLEnd]-1); i++) { newAligned[i] = '.'; }
+                                                       }
+                                               }
+                                                       
+                                               trim->setAligned(newAligned);
+                                       }
+                                       
+                               }
+                       }
+                       
+                       printBlock(leftPiece, rightPiece, leftChimeric, rightChimeric, chimeraFlag, out);
+                       out << endl;
+               }else {  out << querySeq->getName() << "\tno" << endl;  }
+               
+               return trim;
+               
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraSlayer", "print");
+               exit(1);
+       }
+}
+
 #ifdef USE_MPI
 //***************************************************************************************************************
-int ChimeraSlayer::print(MPI_File& out, MPI_File& outAcc) {
+Sequence* ChimeraSlayer::print(MPI_File& out, MPI_File& outAcc, data_results leftPiece, data_results rightPiece) {
        try {
                MPI_Status status;
                bool results = false;
                string outAccString = "";
                string outputString = "";
                
+               Sequence* trim = NULL;
+               
+               if (trimChimera) { 
+                       string aligned = leftPiece.trimQuery.getAligned() + rightPiece.trimQuery.getAligned();
+                       trim = new Sequence(leftPiece.trimQuery.getName(), aligned); 
+               }
+               
+               
+               if ((leftPiece.flag == "yes") || (rightPiece.flag == "yes")) {
+                       
+                       string chimeraFlag = "no";
+                       if (leftPiece.flag == "yes") {
+                               
+                               if(  (leftPiece.results[0].bsa >= minBS && leftPiece.results[0].divr_qla_qrb >= divR)
+                                  ||
+                                  (leftPiece.results[0].bsb >= minBS && leftPiece.results[0].divr_qlb_qra >= divR) ) { chimeraFlag = "yes"; }
+                       }
+                       
+                       if (rightPiece.flag == "yes") {
+                               if ( (rightPiece.results[0].bsa >= minBS && rightPiece.results[0].divr_qla_qrb >= divR)
+                                       ||
+                                       (rightPiece.results[0].bsb >= minBS && rightPiece.results[0].divr_qlb_qra >= divR) ) { chimeraFlag = "yes"; }
+                       }
+                       
+                       bool rightChimeric = false;
+                       bool leftChimeric = false;
+                       
+                       if (chimeraFlag == "yes") {     
+                               //which peice is chimeric or are both
+                               if (rightPiece.flag == "yes") { if ((rightPiece.results[0].bsa >= minBS) || (rightPiece.results[0].bsb >= minBS)) { rightChimeric = true; } }
+                               if (leftPiece.flag == "yes") { if ((leftPiece.results[0].bsa >= minBS) || (leftPiece.results[0].bsb >= minBS))  { leftChimeric = true;  } }
+                               
+                               if (rightChimeric || leftChimeric) {
+                                       cout << querySeq->getName() <<  "\tyes" << endl;
+                                       outAccString += querySeq->getName() + "\n";
+                                       results = true;
+                                       
+                                       //write to accnos file
+                                       int length = outAccString.length();
+                                       char* buf2 = new char[length];
+                                       memcpy(buf2, outAccString.c_str(), length);
+                               
+                                       MPI_File_write_shared(outAcc, buf2, length, MPI_CHAR, &status);
+                                       delete buf2;
+                                       
+                                       if (trimChimera) {  
+                                               string newAligned = trim->getAligned();
+                                               
+                                               //right side is fine so keep that
+                                               if ((leftChimeric) && (!rightChimeric)) {
+                                                       for (int i = 0; i < leftPiece.spotMap[leftPiece.results[0].winREnd]; i++) { newAligned[i] = '.'; } 
+                                               }else if ((!leftChimeric) && (rightChimeric)) { //leftside is fine so keep that
+                                                       for (int i = (rightPiece.spotMap[rightPiece.results[0].winLStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                               }else { //both sides are chimeric, keep longest piece
+                                                       
+                                                       int lengthLeftLeft = leftPiece.spotMap[leftPiece.results[0].winLEnd] - leftPiece.spotMap[leftPiece.results[0].winLStart];
+                                                       int lengthLeftRight = leftPiece.spotMap[leftPiece.results[0].winREnd] - leftPiece.spotMap[leftPiece.results[0].winRStart];
+                                                       
+                                                       int longest = 1; // leftleft = 1, leftright = 2, rightleft = 3 rightright = 4
+                                                       int length = lengthLeftLeft;
+                                                       if (lengthLeftLeft < lengthLeftRight) { longest = 2;  length = lengthLeftRight; }
+                                                       
+                                                       int lengthRightLeft = rightPiece.spotMap[rightPiece.results[0].winLEnd] - rightPiece.spotMap[rightPiece.results[0].winLStart];
+                                                       int lengthRightRight = rightPiece.spotMap[rightPiece.results[0].winREnd] - rightPiece.spotMap[rightPiece.results[0].winRStart];
+                                                       
+                                                       if (lengthRightLeft > length) { longest = 3; length = lengthRightLeft;  }
+                                                       if (lengthRightRight > length) { longest = 4; }
+                                                       
+                                                       if (longest == 1) { //leftleft
+                                                               for (int i = (leftPiece.spotMap[leftPiece.results[0].winRStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                                       }else if (longest == 2) { //leftright
+                                                               //get rid of leftleft
+                                                               for (int i = (leftPiece.spotMap[leftPiece.results[0].winLStart]-1); i < (leftPiece.spotMap[leftPiece.results[0].winLEnd]-1); i++) { newAligned[i] = '.'; }
+                                                               //get rid of right
+                                                               for (int i = (rightPiece.spotMap[rightPiece.results[0].winLStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                                       }else if (longest == 3) { //rightleft
+                                                               //get rid of left
+                                                               for (int i = 0; i < leftPiece.spotMap[leftPiece.results[0].winREnd]; i++) { newAligned[i] = '.'; } 
+                                                               //get rid of rightright
+                                                               for (int i = (rightPiece.spotMap[rightPiece.results[0].winRStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                                       }else { //rightright
+                                                               //get rid of left
+                                                               for (int i = 0; i < leftPiece.spotMap[leftPiece.results[0].winREnd]; i++) { newAligned[i] = '.'; } 
+                                                               //get rid of rightleft
+                                                               for (int i = (rightPiece.spotMap[rightPiece.results[0].winLStart]-1); i < (rightPiece.spotMap[rightPiece.results[0].winLEnd]-1); i++) { newAligned[i] = '.'; }
+                                                       }
+                                               }
+                                               
+                                               trim->setAligned(newAligned);
+                                       }
+                                       
+                               }
+                       }
+                       
+                       outputString = getBlock(leftPiece, rightPiece, leftChimeric, rightChimeric, chimeraFlag);
+                       outputString += "\n";
+               
+                       //write to output file
+                       int length = outputString.length();
+                       char* buf = new char[length];
+                       memcpy(buf, outputString.c_str(), length);
+                               
+                       MPI_File_write_shared(out, buf, length, MPI_CHAR, &status);
+                       delete buf;
+
+               }else {  
+                       outputString += querySeq->getName() + "\tno\n";  
+       
+                       //write to output file
+                       int length = outputString.length();
+                       char* buf = new char[length];
+                       memcpy(buf, outputString.c_str(), length);
+                               
+                       MPI_File_write_shared(out, buf, length, MPI_CHAR, &status);
+                       delete buf;
+               }
+               
+               
+               return trim;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraSlayer", "print");
+               exit(1);
+       }
+}
+//***************************************************************************************************************
+Sequence* ChimeraSlayer::print(MPI_File& out, MPI_File& outAcc) {
+       try {
+               MPI_Status status;
+               bool results = false;
+               string outAccString = "";
+               string outputString = "";
+               
+               Sequence* trim = NULL;
+               if (trimChimera) { trim = trimQuery; }
+               
                if (chimeraFlags == "yes") {
                        string chimeraFlag = "no";
                        if(  (chimeraResults[0].bsa >= minBS && chimeraResults[0].divr_qla_qrb >= divR)
@@ -468,37 +726,49 @@ int ChimeraSlayer::print(MPI_File& out, MPI_File& outAcc) {
                                        int length = outAccString.length();
                                        char* buf2 = new char[length];
                                        memcpy(buf2, outAccString.c_str(), length);
-                               
+                                       
                                        MPI_File_write_shared(outAcc, buf2, length, MPI_CHAR, &status);
                                        delete buf2;
+                                       
+                                       if (trimChimera) {  
+                                               int lengthLeft = spotMap[chimeraResults[0].winLEnd] - spotMap[chimeraResults[0].winLStart];
+                                               int lengthRight = spotMap[chimeraResults[0].winREnd] - spotMap[chimeraResults[0].winRStart];
+                                               
+                                               string newAligned = trim->getAligned();
+                                               if (lengthLeft > lengthRight) { //trim right
+                                                       for (int i = (spotMap[chimeraResults[0].winRStart]-1); i < newAligned.length(); i++) { newAligned[i] = '.'; }
+                                               }else { //trim left
+                                                       for (int i = 0; i < (spotMap[chimeraResults[0].winLEnd]-1); i++) { newAligned[i] = '.'; }
+                                               }
+                                               trim->setAligned(newAligned);   
+                                       }
                                }
                        }
                        
                        outputString = getBlock(chimeraResults[0], chimeraFlag);
                        outputString += "\n";
-       //cout << outputString << endl;         
+                       
                        //write to output file
                        int length = outputString.length();
                        char* buf = new char[length];
                        memcpy(buf, outputString.c_str(), length);
-                               
+                       
                        MPI_File_write_shared(out, buf, length, MPI_CHAR, &status);
                        delete buf;
-
+                       
                }else {  
                        outputString += querySeq->getName() + "\tno\n";  
-       //cout << outputString << endl;
+                       
                        //write to output file
                        int length = outputString.length();
                        char* buf = new char[length];
                        memcpy(buf, outputString.c_str(), length);
-                               
+                       
                        MPI_File_write_shared(out, buf, length, MPI_CHAR, &status);
                        delete buf;
                }
                
-               
-               return results;
+               return trim;
        }
        catch(exception& e) {
                m->errorOut(e, "ChimeraSlayer", "print");
@@ -510,17 +780,24 @@ int ChimeraSlayer::print(MPI_File& out, MPI_File& outAcc) {
 //***************************************************************************************************************
 int ChimeraSlayer::getChimeras(Sequence* query) {
        try {
+               if (trimChimera) {
+                       trimQuery = new Sequence(query->getName(), query->getAligned());
+                       printResults.trimQuery = *trimQuery; 
+               }
+               
                chimeraFlags = "no";
+               printResults.flag = "no";
 
                //filter query
                spotMap = runFilter(query);     
+               printResults.spotMap = spotMap;
                
                querySeq = query;
                
                //you must create a template
                vector<Sequence*> thisTemplate;
                if (templateFileName != "self") { thisTemplate = templateSeqs; }
-               else { thisTemplate = getTemplate(query); } //fills thistemplate and creates the databases
+               else { thisTemplate = getTemplate(query); } //fills this template and creates the databases
                
                if (m->control_pressed) {  return 0;  }
                
@@ -536,12 +813,13 @@ int ChimeraSlayer::getChimeras(Sequence* query) {
                }
        
                if (m->control_pressed) {  return 0;  }
-               
+
                string chimeraFlag = maligner.getResults(query, decalc);
+               
                if (m->control_pressed) {  return 0;  }
+               
                vector<results> Results = maligner.getOutput();
-                       
-               //found in testing realigning only made things worse
+       
                if (realign) {
                        ChimeraReAligner realigner(thisTemplate, match, misMatch);
                        realigner.reAlign(query, Results);
@@ -569,9 +847,7 @@ int ChimeraSlayer::getChimeras(Sequence* query) {
                        }
                        
                        for (itDup = removeDups.begin(); itDup != removeDups.end(); itDup++) {
-                               //Sequence* seq = getSequence(itDup->first); //makes copy so you can filter and mask and not effect template
                                itSeq = parentNameSeq.find(itDup->first);
-//cout << itDup->first << itSeq->second << endl;
                                Sequence* seq = new Sequence(itDup->first, itSeq->second);
                                
                                SeqDist member;
@@ -596,6 +872,7 @@ int ChimeraSlayer::getChimeras(Sequence* query) {
                        
                        //put seqs into vector to send to slayer
                        vector<Sequence*> seqsForSlayer;
+                       
                        for (int k = 0; k < seqs.size(); k++) {  seqsForSlayer.push_back(seqs[k].seq);  }
                        
                        //mask then send to slayer...
@@ -614,7 +891,7 @@ int ChimeraSlayer::getChimeras(Sequence* query) {
                        }
                        
                        if (m->control_pressed) {  for (int k = 0; k < seqs.size(); k++) {  delete seqs[k].seq;   }  return 0;  }
-                       
+
                        //send to slayer
                        chimeraFlags = slayer.getResults(query, seqsForSlayer);
                        if (m->control_pressed) {  return 0;  }
@@ -622,11 +899,12 @@ int ChimeraSlayer::getChimeras(Sequence* query) {
                        
                        //free memory
                        for (int k = 0; k < seqs.size(); k++) {  delete seqs[k].seq;   }
+                       
+                       printResults.spotMap = spotMap;
+                       printResults.flag = chimeraFlags;
+                       printResults.results = chimeraResults;
                }
                
-               //delete maligner;
-               //delete slayer;
-               
                return 0;
        }
        catch(exception& e) {
@@ -637,32 +915,66 @@ int ChimeraSlayer::getChimeras(Sequence* query) {
 //***************************************************************************************************************
 void ChimeraSlayer::printBlock(data_struct data, string flag, ostream& out){
        try {
-       //out << ":)\n";
-               
                out << querySeq->getName() << '\t';
                out << data.parentA.getName() << "\t" << data.parentB.getName()  << '\t';
-               //out << "Left Window: " << spotMap[data.winLStart] << " " << spotMap[data.winLEnd] << endl;
-               //out << "Right Window: " << spotMap[data.winRStart] << " " << spotMap[data.winREnd] << endl;
-               
+       
                out << data.divr_qla_qrb << '\t' << data.qla_qrb << '\t' << data.bsa << '\t';
                out << data.divr_qlb_qra << '\t' << data.qlb_qra << '\t' << data.bsb << '\t';
                
                out << flag << '\t' << spotMap[data.winLStart] << "-" << spotMap[data.winLEnd] << '\t' << spotMap[data.winRStart] << "-" << spotMap[data.winREnd] << '\t';
                
-               //out << "Similarity of parents: " << data.ab << endl;
-               //out << "Similarity of query to parentA: " << data.qa << endl;
-               //out << "Similarity of query to parentB: " << data.qb << endl;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraSlayer", "printBlock");
+               exit(1);
+       }
+}
+//***************************************************************************************************************
+void ChimeraSlayer::printBlock(data_results leftdata, data_results rightdata, bool leftChimeric, bool rightChimeric, string flag, ostream& out){
+       try {
+               
+               if ((leftChimeric) && (!rightChimeric)) { //print left
+                       out << querySeq->getName() << '\t';
+                       out << leftdata.results[0].parentA.getName() << "\t" << leftdata.results[0].parentB.getName()  << '\t';
+                       
+                       out << leftdata.results[0].divr_qla_qrb << '\t' << leftdata.results[0].qla_qrb << '\t' << leftdata.results[0].bsa << '\t';
+                       out << leftdata.results[0].divr_qlb_qra << '\t' << leftdata.results[0].qlb_qra << '\t' << leftdata.results[0].bsb << '\t';
                
+                       out << flag << '\t' << leftdata.spotMap[leftdata.results[0].winLStart] << "-" << leftdata.spotMap[leftdata.results[0].winLEnd] << '\t' << leftdata.spotMap[leftdata.results[0].winRStart] << "-" << leftdata.spotMap[leftdata.results[0].winREnd] << '\t';
                
-               //out << "Per_id(QL,A): " << data.qla << endl;
-               //out << "Per_id(QL,B): " << data.qlb << endl;
-               //out << "Per_id(QR,A): " << data.qra << endl;
-               //out << "Per_id(QR,B): " << data.qrb << endl;
-
+               }else if ((!leftChimeric) && (rightChimeric)) {  //print right
+                       out << querySeq->getName() << '\t';
+                       out << rightdata.results[0].parentA.getName() << "\t" << rightdata.results[0].parentB.getName()  << '\t';
+                       
+                       out << rightdata.results[0].divr_qla_qrb << '\t' << rightdata.results[0].qla_qrb << '\t' << rightdata.results[0].bsa << '\t';
+                       out << rightdata.results[0].divr_qlb_qra << '\t' << rightdata.results[0].qlb_qra << '\t' << rightdata.results[0].bsb << '\t';
+                       
+                       out << flag << '\t' << rightdata.spotMap[rightdata.results[0].winLStart] << "-" << rightdata.spotMap[rightdata.results[0].winLEnd] << '\t' << rightdata.spotMap[rightdata.results[0].winRStart] << "-" << rightdata.spotMap[rightdata.results[0].winREnd] << '\t';                      
+                       
+               }else  { //print both results
+                       if (leftdata.flag == "yes") {
+                               out << querySeq->getName() + "_LEFT" << '\t';
+                               out << leftdata.results[0].parentA.getName() << "\t" << leftdata.results[0].parentB.getName()  << '\t';
+                               
+                               out << leftdata.results[0].divr_qla_qrb << '\t' << leftdata.results[0].qla_qrb << '\t' << leftdata.results[0].bsa << '\t';
+                               out << leftdata.results[0].divr_qlb_qra << '\t' << leftdata.results[0].qlb_qra << '\t' << leftdata.results[0].bsb << '\t';
+                               
+                               out << flag << '\t' << leftdata.spotMap[leftdata.results[0].winLStart] << "-" << leftdata.spotMap[leftdata.results[0].winLEnd] << '\t' << leftdata.spotMap[leftdata.results[0].winRStart] << "-" << leftdata.spotMap[leftdata.results[0].winREnd] << '\t';
+                       }
+                       
+                       if (rightdata.flag == "yes") {
+                               if (leftdata.flag == "yes") { out << endl; }
+                               
+                               out << querySeq->getName() + "_RIGHT"<< '\t';
+                               out << rightdata.results[0].parentA.getName() << "\t" << rightdata.results[0].parentB.getName()  << '\t';
+                               
+                               out << rightdata.results[0].divr_qla_qrb << '\t' << rightdata.results[0].qla_qrb << '\t' << rightdata.results[0].bsa << '\t';
+                               out << rightdata.results[0].divr_qlb_qra << '\t' << rightdata.results[0].qlb_qra << '\t' << rightdata.results[0].bsb << '\t';
+                               
+                               out << flag << '\t' << rightdata.spotMap[rightdata.results[0].winLStart] << "-" << rightdata.spotMap[rightdata.results[0].winLEnd] << '\t' << rightdata.spotMap[rightdata.results[0].winRStart] << "-" << rightdata.spotMap[rightdata.results[0].winREnd] << '\t';                      
                
-               //out << "DeltaL: " << (data.qla - data.qlb) << endl;
-               //out << "DeltaR: " << (data.qra - data.qrb) << endl;
-
+                       }
+               }
        }
        catch(exception& e) {
                m->errorOut(e, "ChimeraSlayer", "printBlock");
@@ -670,6 +982,62 @@ void ChimeraSlayer::printBlock(data_struct data, string flag, ostream& out){
        }
 }
 //***************************************************************************************************************
+string ChimeraSlayer::getBlock(data_results leftdata, data_results rightdata, bool leftChimeric, bool rightChimeric, string flag){
+       try {
+               
+               string out = "";
+               
+               if ((leftChimeric) && (!rightChimeric)) { //get left
+                       out += querySeq->getName() + "\t";
+                       out += leftdata.results[0].parentA.getName() + "\t" + leftdata.results[0].parentB.getName() + "\t";
+                       
+                       out += toString(leftdata.results[0].divr_qla_qrb) + "\t" + toString(leftdata.results[0].qla_qrb) + "\t" + toString(leftdata.results[0].bsa) + "\t";
+                       out += toString(leftdata.results[0].divr_qlb_qra) + "\t" + toString(leftdata.results[0].qlb_qra) + "\t" + toString(leftdata.results[0].bsb) + "\t";
+                       
+                       out += flag + "\t" + toString(leftdata.spotMap[leftdata.results[0].winLStart]) + "-" + toString(leftdata.spotMap[leftdata.results[0].winLEnd]) + "\t" + toString(leftdata.spotMap[leftdata.results[0].winRStart]) + "-" + toString(leftdata.spotMap[leftdata.results[0].winREnd]) + "\t";
+                       
+               }else if ((!leftChimeric) && (rightChimeric)) {  //print right
+                       out += querySeq->getName() + "\t";
+                       out += rightdata.results[0].parentA.getName() + "\t" + rightdata.results[0].parentB.getName()  + "\t";
+                       
+                       out += toString(rightdata.results[0].divr_qla_qrb) + "\t" + toString(rightdata.results[0].qla_qrb) + "\t" + toString(rightdata.results[0].bsa) + "\t";
+                       out += toString(rightdata.results[0].divr_qlb_qra) + "\t" + toString(rightdata.results[0].qlb_qra) + "\t" + toString(rightdata.results[0].bsb) + "\t";
+                       
+                       out += flag + "\t" + toString(rightdata.spotMap[rightdata.results[0].winLStart]) + "-" + toString(rightdata.spotMap[rightdata.results[0].winLEnd]) + "\t" + toString(rightdata.spotMap[rightdata.results[0].winRStart]) + "-" + toString(rightdata.spotMap[rightdata.results[0].winREnd]) + "\t";                       
+                       
+               }else  { //print both results
+                       
+                       if (leftdata.flag == "yes") {
+                               out += querySeq->getName() + "_LEFT\t";
+                               out += leftdata.results[0].parentA.getName() + "\t" + leftdata.results[0].parentB.getName() + "\t";
+                               
+                               out += toString(leftdata.results[0].divr_qla_qrb) + "\t" + toString(leftdata.results[0].qla_qrb) + "\t" + toString(leftdata.results[0].bsa) + "\t";
+                               out += toString(leftdata.results[0].divr_qlb_qra) + "\t" + toString(leftdata.results[0].qlb_qra) + "\t" + toString(leftdata.results[0].bsb) + "\t";
+                               
+                               out += flag + "\t" + toString(leftdata.spotMap[leftdata.results[0].winLStart]) + "-" + toString(leftdata.spotMap[leftdata.results[0].winLEnd]) + "\t" + toString(leftdata.spotMap[leftdata.results[0].winRStart]) + "-" + toString(leftdata.spotMap[leftdata.results[0].winREnd]) + "\t";
+                       }
+                       
+                       if (rightdata.flag == "yes") {
+                               if (leftdata.flag == "yes") { out += "\n"; }
+                               out +=  querySeq->getName() + "_RIGHT\t";
+                               out += rightdata.results[0].parentA.getName() + "\t" + rightdata.results[0].parentB.getName()  + "\t";
+                               
+                               out += toString(rightdata.results[0].divr_qla_qrb) + "\t" + toString(rightdata.results[0].qla_qrb) + "\t" + toString(rightdata.results[0].bsa) + "\t";
+                               out += toString(rightdata.results[0].divr_qlb_qra) + "\t" + toString(rightdata.results[0].qlb_qra) + "\t" + toString(rightdata.results[0].bsb) + "\t";
+                               
+                               out += flag + "\t" + toString(rightdata.spotMap[rightdata.results[0].winLStart]) + "-" + toString(rightdata.spotMap[rightdata.results[0].winLEnd]) + "\t" + toString(rightdata.spotMap[rightdata.results[0].winRStart]) + "-" + toString(rightdata.spotMap[rightdata.results[0].winREnd]) + "\t";                       
+                       }
+               }
+               
+               return out;
+               
+       }
+       catch(exception& e) {
+               m->errorOut(e, "ChimeraSlayer", "getBlock");
+               exit(1);
+       }
+}
+//***************************************************************************************************************
 string ChimeraSlayer::getBlock(data_struct data, string flag){
        try {