#include "chimeraseqscommand.h"
#include "bellerophon.h"
#include "pintail.h"
+#include "ccode.h"
+#include "chimeracheckrdp.h"
+
//***************************************************************************************************************
else {
//valid paramters for this command
- string Array[] = {"fasta", "filter", "correction", "processors", "method", "window", "increment", "template", "conservation", "quantiles" };
+ string Array[] = {"fasta", "filter", "correction", "processors", "method", "window", "increment", "template", "conservation", "quantile", "mask", "numwanted", "ksize", "svg", "name" };
vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
OptionParser parser(option);
if (consfile == "not open") { abort = true; }
else if (consfile == "not found") { consfile = ""; }
- quanfile = validParameter.validFile(parameters, "quantiles", true);
+ quanfile = validParameter.validFile(parameters, "quantile", true);
if (quanfile == "not open") { abort = true; }
- else if (quanfile == "not found") { consfile = ""; }
+ else if (quanfile == "not found") { quanfile = ""; }
+ namefile = validParameter.validFile(parameters, "name", true);
+ if (namefile == "not open") { abort = true; }
+ else if (namefile == "not found") { namefile = ""; }
+ maskfile = validParameter.validFile(parameters, "mask", false);
+ if (maskfile == "not found") { maskfile = ""; }
+ else if (maskfile != "default") {
+ ifstream in;
+ int ableToOpen = openInputFile(maskfile, in);
+ if (ableToOpen == 1) { abort = true; }
+ in.close();
+ }
+
+ method = validParameter.validFile(parameters, "method", false); if (method == "not found") { method = "pintail"; }
+
string temp;
- temp = validParameter.validFile(parameters, "filter", false); if (temp == "not found") { temp = "T"; }
+ temp = validParameter.validFile(parameters, "filter", false); if (temp == "not found") { temp = "F"; }
filter = isTrue(temp);
temp = validParameter.validFile(parameters, "correction", false); if (temp == "not found") { temp = "T"; }
temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found") { temp = "1"; }
convert(temp, processors);
+ temp = validParameter.validFile(parameters, "ksize", false); if (temp == "not found") { temp = "7"; }
+ convert(temp, ksize);
+
+ temp = validParameter.validFile(parameters, "svg", false); if (temp == "not found") { temp = "F"; }
+ svg = isTrue(temp);
+
temp = validParameter.validFile(parameters, "window", false); if (temp == "not found") { temp = "0"; }
convert(temp, window);
- temp = validParameter.validFile(parameters, "increment", false); if (temp == "not found") { temp = "25"; }
+ temp = validParameter.validFile(parameters, "increment", false);
+ if ((temp == "not found") && (method == "chimeracheck")) { temp = "10"; }
+ else if (temp == "not found") { temp = "25"; }
convert(temp, increment);
-
- method = validParameter.validFile(parameters, "method", false); if (method == "not found") { method = "pintail"; }
- if ((method == "pintail") && (templatefile == "") && (consfile == "")) { mothurOut("You must provide a template or conservation file with the pintail method."); mothurOutEndLine(); abort = true; }
+ temp = validParameter.validFile(parameters, "numwanted", false); if (temp == "not found") { temp = "20"; }
+ convert(temp, numwanted);
+
+
+
+ if (((method != "bellerophon")) && (templatefile == "")) { mothurOut("You must provide a template file with the pintail, ccode or chimeracheck methods."); mothurOutEndLine(); abort = true; }
}
void ChimeraSeqsCommand::help(){
try {
- mothurOut("The chimera.seqs command reads a fastafile and creates a sorted priority score list of potentially chimeric sequences (ideally, the sequences should already be aligned).\n");
- mothurOut("The chimera.seqs command parameters are fasta, filter, correction, processors and method. fasta is required.\n");
- mothurOut("The filter parameter allows you to specify if you would like to apply a 50% soft filter. The default is false. \n");
- mothurOut("The correction parameter allows you to put more emphasis on the distance between highly similar sequences and less emphasis on the differences between remote homologs. The default is true. \n");
+
+ //"fasta", "filter", "correction", "processors", "method", "window", "increment", "template", "conservation", "quantile", "mask", "numwanted", "ksize", "svg", "name"
+ //mothurOut("chimera.seqs ASSUMES that your sequences are ALIGNED and if using a template that the template file sequences are the same length as the fasta file sequences.\n\n");
+ mothurOut("The chimera.seqs command reads a fastafile and creates list of potentially chimeric sequences.\n");
+ mothurOut("The chimera.seqs command parameters are fasta, filter, correction, processors, mask, method, window, increment, template, conservation, quantile, numwanted, ksize, svg, name.\n");
+ mothurOut("The fasta parameter is always required and template is required if using pintail, ccode or chimeracheck.\n");
+ mothurOut("The filter parameter allows you to specify if you would like to apply a vertical and 50% soft filter. \n");
+ mothurOut("The correction parameter allows you to put more emphasis on the distance between highly similar sequences and less emphasis on the differences between remote homologs.\n");
mothurOut("The processors parameter allows you to specify how many processors you would like to use. The default is 1. \n");
- mothurOut("The method parameter allows you to specify the method for finding chimeric sequences. The default is pintail. \n");
+ mothurOut("The method parameter allows you to specify the method for finding chimeric sequences. The default is pintail. Options include bellerophon, ccode and chimeracheck \n");
+ mothurOut("The mask parameter allows you to specify a file containing one sequence you wish to use as a mask for the your sequences. \n");
+ mothurOut("The window parameter allows you to specify the window size for searching for chimeras. \n");
+ mothurOut("The increment parameter allows you to specify how far you move each window while finding chimeric sequences.\n");
+ mothurOut("The template parameter allows you to enter a template file containing known non-chimeric sequences. \n");
+ mothurOut("The conservation parameter allows you to enter a frequency file containing the highest bases frequency at each place in the alignment.\n");
+ mothurOut("The quantile parameter allows you to enter a file containing quantiles for a template files sequences.\n");
+ mothurOut("The numwanted parameter allows you to specify how many sequences you would each query sequence compared with.\n");
+ mothurOut("The ksize parameter allows you to input kmersize. \n");
+ mothurOut("The svg parameter allows you to specify whether or not you would like a svg file outputted for each query sequence.\n");
+ mothurOut("The name parameter allows you to enter a file containing names of sequences you would like .svg files for.\n");
+ mothurOut("NOT ALL PARAMETERS ARE USED BY ALL METHODS. Please look below for method specifics.\n\n");
+ mothurOut("Details for each method: \n");
+ mothurOut("\tpintail: \n");
+ mothurOut("\t\tparameters: fasta=required, template=required, filter=F, mask=no mask, processors=1, window=300, increment=25, conservation=not required, but will improve speed, quantile=not required, but will greatly improve speed. \n");
+ mothurOut("\t\tIf you have run chimera.seqs using pintail a .quan and .freq file will be created for your template, if you have not provided them for use in future command executions.\n");
+ mothurOut("\tbellerophon: \n");
+ mothurOut("\t\tparameters: fasta=required, filter=F, processors=1, window=1/4 length of seq, increment=25, correction=T. \n");
+ mothurOut("\tccode: \n");
+ mothurOut("\t\tparameters: fasta=required, template=required, filter=F, mask=no mask, processors=1, window=10% of length, numwanted=20\n");
+ mothurOut("\tchimeracheck: \n");
+ mothurOut("\t\tparameters: fasta=required, template=required, processors=1, increment=10, ksize=7, svg=F, name=none\n\n");
mothurOut("The chimera.seqs command should be in the following format: \n");
mothurOut("chimera.seqs(fasta=yourFastaFile, filter=yourFilter, correction=yourCorrection, processors=yourProcessors, method=bellerophon) \n");
- mothurOut("Example: chimera.seqs(fasta=AD.align, filter=True, correction=true, processors=2, method=yourMethod) \n");
+ mothurOut("Example: chimera.seqs(fasta=AD.align, filter=True, correction=true, method=bellerophon, window=200) \n");
mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
}
catch(exception& e) {
if (abort == true) { return 0; }
- if (method == "bellerophon") { chimera = new Bellerophon(fastafile); }
- else if (method == "pintail") { chimera = new Pintail(fastafile, templatefile);
- //saves time to avoid generating it
- if (consfile != "") { chimera->setCons(consfile); }
- else { chimera->setCons(""); }
-
- //saves time to avoid generating it
- if (quanfile != "") { chimera->setQuantiles(quanfile); }
- else { chimera->setQuantiles(""); }
- }else { mothurOut("Not a valid method."); mothurOutEndLine(); return 0; }
+ if (method == "bellerophon") { chimera = new Bellerophon(fastafile); }
+ else if (method == "pintail") { chimera = new Pintail(fastafile, templatefile); }
+ else if (method == "ccode") { chimera = new Ccode(fastafile, templatefile); }
+ else if (method == "chimeracheck") { chimera = new ChimeraCheckRDP(fastafile, templatefile); }
+ else { mothurOut("Not a valid method."); mothurOutEndLine(); return 0; }
//set user options
+ if (maskfile == "default") { mothurOut("I am using the default 236627 EU009184.1 Shigella dysenteriae str. FBD013."); mothurOutEndLine(); }
+
+ //saves time to avoid generating it
+ chimera->setCons(consfile);
+
+ //saves time to avoid generating it
+ chimera->setQuantiles(quanfile);
+
+ chimera->setMask(maskfile);
chimera->setFilter(filter);
chimera->setCorrection(correction);
chimera->setProcessors(processors);
chimera->setWindow(window);
chimera->setIncrement(increment);
+ chimera->setNumWanted(numwanted);
+ chimera->setKmerSize(ksize);
+ chimera->setSVG(svg);
+ chimera->setName(namefile);
//find chimeras
chimera->getChimeras();
- string outputFileName = getRootName(fastafile) + method + ".chimeras";
+ string outputFileName = getRootName(fastafile) + method + maskfile + ".chimeras";
ofstream out;
openOutputFile(outputFileName, out);