/**************************************************************************************************/
BlastDB::BlastDB() : Database() {
-
- globaldata = GlobalData::getInstance();
- count = 0;
-
- int randNumber = rand();
- dbFileName = toString(randNumber) + ".template.unaligned.fasta";
- queryFileName = toString(randNumber) + ".candidate.unaligned.fasta";
- blastFileName = toString(randNumber) + ".blast";
+ try {
+ globaldata = GlobalData::getInstance();
+ count = 0;
+ int randNumber = rand();
+ dbFileName = toString(randNumber) + ".template.unaligned.fasta";
+ queryFileName = toString(randNumber) + ".candidate.unaligned.fasta";
+ blastFileName = toString(randNumber) + ".blast";
+ }
+ catch(exception& e) {
+ m->errorOut(e, "BlastDB", "BlastDB");
+ exit(1);
+ }
}
/**************************************************************************************************/
BlastDB::~BlastDB(){
- remove(queryFileName.c_str()); // let's clean stuff up and remove the temp files
- remove(dbFileName.c_str()); // let's clean stuff up and remove the temp files
- remove(blastFileName.c_str()); // let's clean stuff up and remove the temp files
+ try{
+ remove(queryFileName.c_str()); // let's clean stuff up and remove the temp files
+ remove(dbFileName.c_str()); // let's clean stuff up and remove the temp files
+ remove((dbFileName+".nsq").c_str()); // let's clean stuff up and remove the temp files
+ remove((dbFileName+".nsi").c_str()); // let's clean stuff up and remove the temp files
+ remove((dbFileName+".nsd").c_str()); // let's clean stuff up and remove the temp files
+ remove((dbFileName+".nin").c_str()); // let's clean stuff up and remove the temp files
+ remove((dbFileName+".nhr").c_str()); // let's clean stuff up and remove the temp files
+ remove(blastFileName.c_str()); // let's clean stuff up and remove the temp files
+ }
+ catch(exception& e) {
+ m->errorOut(e, "BlastDB", "~BlastDB");
+ exit(1);
+ }
}
/**************************************************************************************************/
//assumes you have added all the template sequences using the addSequence function and run generateDB.
vector<int> topMatches;
ofstream queryFile;
- openOutputFile((queryFileName+seq->getName()), queryFile);
+ m->openOutputFile((queryFileName+seq->getName()), queryFile);
queryFile << '>' << seq->getName() << endl;
queryFile << seq->getUnaligned() << endl;
queryFile.close();
system(blastCommand.c_str());
ifstream m8FileHandle;
- openInputFile(blastFileName+seq->getName(), m8FileHandle, "no error");
+ m->openInputFile(blastFileName+seq->getName(), m8FileHandle, "no error");
string dummy;
int templateAccession;
- gobble(m8FileHandle);
+ m->gobble(m8FileHandle);
while(!m8FileHandle.eof()){
m8FileHandle >> dummy >> templateAccession >> searchScore;
//get rest of junk in line
while (!m8FileHandle.eof()) { char c = m8FileHandle.get(); if (c == 10 || c == 13){ break; } }
- gobble(m8FileHandle);
+ m->gobble(m8FileHandle);
topMatches.push_back(templateAccession);
}
m8FileHandle.close();
vector<int> topMatches;
ofstream queryFile;
- openOutputFile((queryFileName+seq->getName()), queryFile);
+ m->openOutputFile((queryFileName+seq->getName()), queryFile);
queryFile << '>' << seq->getName() << endl;
queryFile << seq->getUnaligned() << endl;
queryFile.close();
system(blastCommand.c_str());
ifstream m8FileHandle;
- openInputFile(blastFileName+seq->getName(), m8FileHandle, "no error");
+ m->openInputFile(blastFileName+seq->getName(), m8FileHandle, "no error");
string dummy;
int templateAccession;
- gobble(m8FileHandle);
+ m->gobble(m8FileHandle);
while(!m8FileHandle.eof()){
m8FileHandle >> dummy >> templateAccession >> searchScore;
//get rest of junk in line
while (!m8FileHandle.eof()) { char c = m8FileHandle.get(); if (c == 10 || c == 13){ break; } }
- gobble(m8FileHandle);
+ m->gobble(m8FileHandle);
topMatches.push_back(templateAccession);
//cout << templateAccession << endl;
}
try {
ofstream unalignedFastaFile;
- openOutputFileAppend(dbFileName, unalignedFastaFile);
+ m->openOutputFileAppend(dbFileName, unalignedFastaFile);
// generating a fasta file with unaligned template
unalignedFastaFile << '>' << count << endl; // sequences, which will be input to formatdb