/**************************************************************************************************/
-BlastDB::BlastDB(float gO, float gE, float m, float mM) : Database(),
+BlastDB::BlastDB(string tag, float gO, float gE, float m, float mM) : Database(),
gapOpen(gO), gapExtend(gE), match(m), misMatch(mM) {
count = 0;
int randNumber = rand();
- dbFileName = toString(randNumber) + ".template.unaligned.fasta";
- queryFileName = toString(randNumber) + ".candidate.unaligned.fasta";
- blastFileName = toString(randNumber) + ".blast";
+ dbFileName = tag + toString(randNumber) + ".template.unaligned.fasta";
+ queryFileName = tag + toString(randNumber) + ".candidate.unaligned.fasta";
+ blastFileName = tag + toString(randNumber) + ".blast";
}
/**************************************************************************************************/
queryFile << '>' << seq->getName() << endl;
queryFile << seq->getUnaligned() << endl;
queryFile.close();
-
+ cout << seq->getUnaligned() << endl;
// the goal here is to quickly survey the database to find the closest match. To do this we are using the default
// wordsize used in megablast. I'm sure we're sacrificing accuracy for speed, but anyother way would take way too
// long. With this setting, it seems comparable in speed to the suffix tree approach.
//7000004128189528left 0 100 66 0 0 1 66 61 126 1e-31 131
+
string blastCommand = path + "blast/bin/megablast -e 1e-10 -d " + dbFileName + " -m 8 -b " + toString(n) + " -v " + toString(n); //-W 28 -p blastn
blastCommand += (" -i " + (queryFileName+seq->getName()) + " -o " + blastFileName+seq->getName());
system(blastCommand.c_str());
while(!m8FileHandle.eof()){
m8FileHandle >> dummy >> templateAccession >> searchScore >> numBases >> mismatch >> gap >> startQuery >> endQuery >> startRef >> endRef >> eScore >> score;
- //cout << dummy << '\t' << templateAccession << '\t' << searchScore << '\t' << numBases << '\t' << mismatch << '\t' << gap << '\t' << startQuery << '\t' << endQuery << '\t' << startRef << '\t' << endRef << '\t' << eScore << '\t' << score << endl;
+ cout << dummy << '\t' << templateAccession << '\t' << searchScore << '\t' << numBases << '\t' << mismatch << '\t' << gap << '\t' << startQuery << '\t' << endQuery << '\t' << startRef << '\t' << endRef << '\t' << eScore << '\t' << score << endl;
//get rest of junk in line
//while (!m8FileHandle.eof()) { char c = m8FileHandle.get(); if (c == 10 || c == 13){ break; }else{ cout << c; } } //
m8FileHandle.close();
remove((queryFileName+seq->getName()).c_str());
remove((blastFileName+seq->getName()).c_str());
-//cout << "\n" ;
+cout << "\n" ;
return topMatches;
}
catch(exception& e) {