// wordsize used in megablast. I'm sure we're sacrificing accuracy for speed, but anyother way would take way too
// long. With this setting, it seems comparable in speed to the suffix tree approach.
- string blastCommand = path + "blast/bin/blastall -p blastn -d " + dbFileName + " -b 1 -m 8 -W 28 -v " + toString(n);
+ string blastCommand = path + "blast/bin/blastall -p blastn -d " + dbFileName + " -m 8 -W 28 -v " + toString(n) + " -b " + toString(n);;
blastCommand += (" -i " + queryFileName + " -o " + blastFileName);
system(blastCommand.c_str());
}
m8FileHandle.close();
+ string root = dbFileName;
+ string temp = dbFileName + ".nsq";
+ remove(temp.c_str());
+ temp = dbFileName + ".nsi";
+ remove(temp.c_str());
+
+ temp = dbFileName + ".nsd";
+ remove(temp.c_str());
+
+ temp = dbFileName + ".nin";
+ remove(temp.c_str());
+
+ temp = dbFileName + ".nhr";
+ remove(temp.c_str());
+
+
+
return topMatches;
}
catch(exception& e) {
- errorOut(e, "BlastDB", "findClosestSequences");
+ m->errorOut(e, "BlastDB", "findClosestSequences");
exit(1);
}
}
/**************************************************************************************************/
//assumes you have added all the template sequences using the addSequence function and run generateDB.
-map<int, float> BlastDB::findClosest(Sequence* seq, int n) {
+vector<int> BlastDB::findClosestMegaBlast(Sequence* seq, int n) {
try{
- map<int, float> topMatches;
+ vector<int> topMatches;
ofstream queryFile;
openOutputFile(queryFileName, queryFile);
// wordsize used in megablast. I'm sure we're sacrificing accuracy for speed, but anyother way would take way too
// long. With this setting, it seems comparable in speed to the suffix tree approach.
- string blastCommand = path + "blast/bin/blastall -p blastn -d " + dbFileName + " -m 8 -W 28 -b " + toString(n) + " -v " + toString(n);
+ string blastCommand = path + "blast/bin/megablast -e 1e-10 -d " + dbFileName + " -m 8 -b " + toString(n) + " -v " + toString(n); //-W 28 -p blastn
blastCommand += (" -i " + queryFileName + " -o " + blastFileName);
system(blastCommand.c_str());
ifstream m8FileHandle;
- openInputFile(blastFileName, m8FileHandle);
+ openInputFile(blastFileName, m8FileHandle, "no error");
string dummy;
int templateAccession;
gobble(m8FileHandle);
-//string name = seq->getName();
-//ofstream out;
-//openOutputFileAppend(name, out);
+
while(!m8FileHandle.eof()){
m8FileHandle >> dummy >> templateAccession >> searchScore;
-//out << dummy << '\t' << templateAccession << '\t' << searchScore << endl;
+
//get rest of junk in line
- while (!m8FileHandle.eof()) { char c = m8FileHandle.get();
- //out << c;
- if (c == 10 || c == 13){ break; } }
+ while (!m8FileHandle.eof()) { char c = m8FileHandle.get(); if (c == 10 || c == 13){ break; } }
gobble(m8FileHandle);
- topMatches[templateAccession] = searchScore;
+ topMatches.push_back(templateAccession);
+//cout << templateAccession << endl;
}
m8FileHandle.close();
-//out.close();
+//cout << "\n\n" ;
return topMatches;
}
catch(exception& e) {
- errorOut(e, "BlastDB", "findClosest");
+ m->errorOut(e, "BlastDB", "findClosest");
exit(1);
}
}
count++;
}
catch(exception& e) {
- errorOut(e, "BlastDB", "addSequence");
+ m->errorOut(e, "BlastDB", "addSequence");
exit(1);
}
}
void BlastDB::generateDB() {
try {
- //mothurOut("Generating the temporary BLAST database...\t"); cout.flush();
+ //m->mothurOut("Generating the temporary BLAST database...\t"); cout.flush();
path = globaldata->argv;
path = path.substr(0, (path.find_last_of('m')));
string formatdbCommand = path + "blast/bin/formatdb -p F -o T -i " + dbFileName; // format the database, -o option gives us the ability
system(formatdbCommand.c_str()); // to get the right sequence names, i think. -p F
// option tells formatdb that seqs are DNA, not prot
- //mothurOut("DONE."); mothurOutEndLine(); mothurOutEndLine(); cout.flush();
+ //m->mothurOut("DONE."); m->mothurOutEndLine(); m->mothurOutEndLine(); cout.flush();
}
catch(exception& e) {
- errorOut(e, "BlastDB", "generateDB");
+ m->errorOut(e, "BlastDB", "generateDB");
exit(1);
}
}