#include<string>
#include<algorithm>
#include<sstream>
+#include<iostream>
+#include<vector>
#include "utils.h"
+#include "my_assert.h"
#include "Orientation.h"
#include "LenDist.h"
#include "RSPD.h"
int readLen = read.getReadLength();
int fpos = (dir == 0 ? pos : totLen - pos - readLen); // the aligned position reported in SAM file, should be a coordinate in forward strand
- assert(fpos >= 0 && fpos + readLen <= totLen && readLen <= totLen);
+ general_assert(fpos >= 0, "The alignment of read " + read.getName() + " to transcript " + itos(sid) + " starts at " + itos(fpos) + \
+ " from the forward direction, which should be a non-negative number! " + \
+ "It is possible that the aligner you use gave different read lengths for a same read in SAM file.");
+ general_assert(fpos + readLen <= totLen,"Read " + read.getName() + " is hung over the end of transcript " + itos(sid) + "! " \
+ + "It is possible that the aligner you use gave different read lengths for a same read in SAM file.");
+ general_assert(readLen <= totLen, "Read " + read.getName() + " has length " + itos(readLen) + ", but it is aligned to transcript " \
+ + itos(sid) + ", whose length (" + itos(totLen) + ") is shorter than the read's length!");
+
int seedPos = (dir == 0 ? pos : totLen - pos - seedLen); // the aligned position of the seed in forward strand coordinates
if (seedPos >= fullLen || ref.getMask(seedPos)) return 0.0;
const LenDist& getGLD() { return *gld; }
- void startSimulation(simul*, double*);
- bool simulate(int, SingleReadQ&, int&);
+ void startSimulation(simul*, const std::vector<double>&);
+ bool simulate(READ_INT_TYPE, SingleReadQ&, int&);
void finishSimulation();
//Use it after function 'read' or 'estimateFromReads'
- double* getMW() {
+ const double* getMW() {
assert(mw != NULL);
return mw;
}
static const int read_type = 1;
int M;
- int N[3];
+ READ_INT_TYPE N[3];
Refs *refs;
double mean, sd;
int seedLen;
genReadFileNames(readFN, i, read_type, s, readFs);
ReadReader<SingleReadQ> reader(s, readFs, refs->hasPolyA(), seedLen); // allow calculation of calc_lq() function
- int cnt = 0;
+ READ_INT_TYPE cnt = 0;
while (reader.next(read)) {
if (!read.isLowQuality()) {
mld != NULL ? mld->update(read.getReadLength(), 1.0) : gld->update(read.getReadLength(), 1.0);
if (i == 0) { nqpro->updateC(read.getReadSeq(), read.getQScore()); }
}
else if (verbose && read.getReadLength() < seedLen) {
- printf("Warning: Read %s is ignored due to read length %d < seed length %d!\n", read.getName().c_str(), read.getReadLength(), seedLen);
+ std::cout<< "Warning: Read "<< read.getName()<< " is ignored due to read length "<< read.getReadLength()<< " < seed length "<< seedLen<< "!"<< std::endl;
}
++cnt;
- if (verbose && cnt % 1000000 == 0) { printf("%d READS PROCESSED\n", cnt); }
+ if (verbose && cnt % 1000000 == 0) { std::cout<< cnt<< " READS PROCESSED"<< std::endl; }
}
- if (verbose) { printf("estimateFromReads, N%d finished.\n", i); }
+ if (verbose) { std::cout<< "estimateFromReads, N"<< i<< " finished."<< std::endl; }
}
mld != NULL ? mld->finish() : gld->finish();
fclose(fo);
}
-void SingleQModel::startSimulation(simul* sampler, double* theta) {
+void SingleQModel::startSimulation(simul* sampler, const std::vector<double>& theta) {
this->sampler = sampler;
theta_cdf = new double[M + 1];
nqpro->startSimulation();
}
-bool SingleQModel::simulate(int rid, SingleReadQ& read, int& sid) {
+bool SingleQModel::simulate(READ_INT_TYPE rid, SingleReadQ& read, int& sid) {
int dir, pos, readLen, fragLen;
std::string name;
std::string qual, readseq;