+BlastDB::BlastDB(string tag, float gO, float gE, float mm, float mM) : Database(),
+gapOpen(gO), gapExtend(gE), match(mm), misMatch(mM) {
+ try {
+ count = 0;
+
+ int randNumber = rand();
+ //int randNumber = 12345;
+ string pid = "";
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+ pid += toString(getpid());
+#endif
+
+ dbFileName = tag + pid + toString(randNumber) + ".template.unaligned.fasta";
+ queryFileName = tag + pid + toString(randNumber) + ".candidate.unaligned.fasta";
+ blastFileName = tag + pid + toString(randNumber) + ".blast";
+
+ //make sure blast exists in the write place
+ path = m->argv;
+ string tempPath = path;
+ for (int i = 0; i < path.length(); i++) { tempPath[i] = tolower(path[i]); }
+ path = path.substr(0, (tempPath.find_last_of('m')));
+
+ string formatdbCommand;
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+ formatdbCommand = path + "blast/bin/formatdb"; // format the database, -o option gives us the ability
+#else
+ formatdbCommand = path + "blast\\bin\\formatdb.exe";
+ //wrap entire string in ""
+ //formatdbCommand = "\"" + formatdbCommand + "\"";
+#endif
+
+ //test to make sure formatdb exists
+ ifstream in;
+ formatdbCommand = m->getFullPathName(formatdbCommand);
+ int ableToOpen = m->openInputFile(formatdbCommand, in, "no error"); in.close();
+ if(ableToOpen == 1) { m->mothurOut("[ERROR]: " + formatdbCommand + " file does not exist. mothur requires formatdb.exe to be in the ./blast/bin folder relative to the mothur.exe location."); m->mothurOutEndLine(); m->control_pressed = true; }
+
+ string blastCommand;
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+ blastCommand = path + "blast/bin/blastall"; // format the database, -o option gives us the ability
+#else
+ blastCommand = path + "blast\\bin\\blastall.exe";
+ //wrap entire string in ""
+ //blastCommand = "\"" + blastCommand + "\"";
+#endif
+
+ //test to make sure formatdb exists
+ ifstream in2;
+ blastCommand = m->getFullPathName(blastCommand);
+ ableToOpen = m->openInputFile(blastCommand, in2, "no error"); in2.close();
+ if(ableToOpen == 1) { m->mothurOut("[ERROR]: " + blastCommand + " file does not exist. mothur requires blastall.exe to be in the ./blast/bin folder relative to the mothur.exe location."); m->mothurOutEndLine(); m->control_pressed = true; }
+
+
+ string megablastCommand;
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
+ megablastCommand = path + "blast/bin/megablast"; // format the database, -o option gives us the ability
+#else
+ megablastCommand = path + "blast\\bin\\megablast.exe";
+ //wrap entire string in ""
+ //megablastCommand = "\"" + megablastCommand + "\"";
+#endif
+
+ //test to make sure formatdb exists
+ ifstream in3;
+ megablastCommand = m->getFullPathName(megablastCommand);
+ ableToOpen = m->openInputFile(megablastCommand, in3, "no error"); in3.close();
+ if(ableToOpen == 1) { m->mothurOut("[ERROR]: " + megablastCommand + " file does not exist. mothur requires megablast.exe to be in the ./blast/bin folder relative to the mothur.exe location."); m->mothurOutEndLine(); m->control_pressed = true; }
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "BlastDB", "BlastDB");
+ exit(1);
+ }
+}
+/**************************************************************************************************/