1 #ifndef METASTATSCOMMAND_H
2 #define METASTATSCOMMAND_H
8 * Created by westcott on 9/16/10.
9 * Copyright 2010 Schloss Lab. All rights reserved.
13 #include "command.hpp"
14 #include "inputdata.h"
15 #include "sharedrabundvector.h"
16 #include "mothurmetastats.h"
18 class MetaStatsCommand : public Command {
21 MetaStatsCommand(string);
23 ~MetaStatsCommand() {}
25 vector<string> setParameters();
26 string getCommandName() { return "metastats"; }
27 string getCommandCategory() { return "OTU-Based Approaches"; }
28 string getHelpString();
29 string getCitation() { return "White JR, Nagarajan N, Pop M (2009). Statistical methods for detecting differentially abundant features in clinical metagenomic samples. PLoS Comput Biol 5: e1000352. \nhttp://www.mothur.org/wiki/Metastats"; }
30 string getDescription() { return "detects differentially abundant features in clinical metagenomic samples"; }
33 void help() { m->mothurOut(getHelpString()); }
39 linePair(int i, int j) : start(i), num(j) {}
41 vector<linePair> lines;
45 vector<SharedRAbundVector*> lookup;
47 bool abort, allLines, pickedGroups;
48 set<string> labels; //holds labels to be used
49 string groups, label, outputDir, inputDir, designfile, sets, sharedfile;
50 vector<string> Groups, outputNames, Sets;
51 vector< vector<string> > namesOfGroupCombos;
52 int iters, processors;
55 int process(vector<SharedRAbundVector*>&);
56 int driver(int, int, vector<SharedRAbundVector*>&);
59 /**************************************************************************************************/
60 //custom data structure for threads to use.
61 // This is passed by void pointer so it can be any data type
62 // that can be passed using a single void pointer (LPVOID).
63 struct metastatsData {
64 vector<SharedRAbundVector*> thisLookUp;
65 vector< vector<string> > namesOfGroupCombos;
66 vector<string> designMapGroups;
67 vector<string> outputNames;
76 metastatsData(string sf, string oDir, MothurOut* mout, int st, int en, vector< vector<string> > ns, vector<SharedRAbundVector*> lu, vector<string> dg, int i, float thr) {
82 namesOfGroupCombos = ns;
89 /**************************************************************************************************/
90 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
92 static DWORD WINAPI MyMetastatsThreadFunction(LPVOID lpParam){
93 metastatsData* pDataArray;
94 pDataArray = (metastatsData*)lpParam;
99 for (int c = pDataArray->start; c < (pDataArray->start+pDataArray->num); c++) {
102 string setA = pDataArray->namesOfGroupCombos[c][0];
103 string setB = pDataArray->namesOfGroupCombos[c][1];
106 string outputFileName = pDataArray->outputDir + pDataArray->m->getRootName(pDataArray->m->getSimpleName(pDataArray->sharedfile)) + pDataArray->thisLookUp[0]->getLabel() + "." + setA + "-" + setB + ".metastats";
107 pDataArray->outputNames.push_back(outputFileName);
109 vector< vector<double> > data2; data2.resize(pDataArray->thisLookUp[0]->getNumBins());
111 vector<SharedRAbundVector*> subset;
114 for (int i = 0; i < pDataArray->thisLookUp.size(); i++) {
115 //is this group for a set we want to compare??
116 //sorting the sets by putting setB at the back and setA in the front
117 if (pDataArray->designMapGroups[i] == setB) {
118 subset.push_back(pDataArray->thisLookUp[i]);
120 }else if (pDataArray->designMapGroups[i] == setA) {
121 subset.insert(subset.begin()+setACount, pDataArray->thisLookUp[i]);
126 if ((setACount == 0) || (setBCount == 0)) {
127 pDataArray->m->mothurOut("Missing shared info for " + setA + " or " + setB + ". Skipping comparison."); pDataArray->m->mothurOutEndLine();
128 pDataArray->outputNames.pop_back();
131 for (int j = 0; j < pDataArray->thisLookUp[0]->getNumBins(); j++) {
132 data2[j].resize(subset.size(), 0.0);
133 for (int i = 0; i < subset.size(); i++) {
134 data2[j][i] = (subset[i]->getAbundance(j));
138 pDataArray->m->mothurOut("Comparing " + setA + " and " + setB + "..."); pDataArray->m->mothurOutEndLine();
140 pDataArray->m->mothurOutEndLine();
141 MothurMetastats mothurMeta(pDataArray->threshold, pDataArray->iters);
142 mothurMeta.runMetastats(outputFileName, data2, setACount);
143 pDataArray->m->mothurOutEndLine();
144 pDataArray->m->mothurOutEndLine();
151 catch(exception& e) {
152 pDataArray->m->errorOut(e, "MetaStatsCommand", "MyMetastatsThreadFunction");