5 * Created by westcott on 2/9/11.
6 * Copyright 2011 Schloss Lab. All rights reserved.
10 #include "mantelcommand.h"
11 #include "readphylipvector.h"
14 //**********************************************************************************************************************
15 vector<string> MantelCommand::setParameters(){
17 CommandParameter pphylip1("phylip1", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pphylip1);
18 CommandParameter pphylip2("phylip2", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pphylip2);
19 CommandParameter piters("iters", "Number", "", "1000", "", "", "",false,false); parameters.push_back(piters);
20 CommandParameter pmethod("method", "Multiple", "pearson-spearman-kendall", "pearson", "", "", "",false,false); parameters.push_back(pmethod);
21 CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
22 CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
24 vector<string> myArray;
25 for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
29 m->errorOut(e, "MantelCommand", "setParameters");
33 //**********************************************************************************************************************
34 string MantelCommand::getHelpString(){
36 string helpString = "";
37 helpString += "Sokal, R. R., & Rohlf, F. J. (1995). Biometry, 3rd edn. New York: Freeman.\n";
38 helpString += "The mantel command reads two distance matrices and calculates the mantel correlation coefficient.\n";
39 helpString += "The mantel command parameters are phylip1, phylip2, iters and method. The phylip1 and phylip2 parameters are required. Matrices must be the same size and contain the same names.\n";
40 helpString += "The method parameter allows you to select what method you would like to use. Options are pearson, spearman and kendall. Default=pearson.\n";
41 helpString += "The iters parameter allows you to set number of randomization for the P value. The default is 1000. \n";
42 helpString += "The mantel command should be in the following format: mantel(phylip1=veg.dist, phylip2=env.dist).\n";
43 helpString += "The mantel command outputs a .mantel file.\n";
44 helpString += "Note: No spaces between parameter labels (i.e. phylip1), '=' and parameters (i.e. veg.dist).\n";
48 m->errorOut(e, "MantelCommand", "getHelpString");
52 //**********************************************************************************************************************
53 MantelCommand::MantelCommand(){
55 abort = true; calledHelp = true;
57 vector<string> tempOutNames;
58 outputTypes["mantel"] = tempOutNames;
61 m->errorOut(e, "MantelCommand", "MantelCommand");
65 //**********************************************************************************************************************
66 MantelCommand::MantelCommand(string option) {
68 abort = false; calledHelp = false;
70 //allow user to run help
71 if(option == "help") { help(); abort = true; calledHelp = true; }
72 else if(option == "citation") { citation(); abort = true; calledHelp = true;}
75 vector<string> myArray = setParameters();
77 OptionParser parser(option);
78 map<string, string> parameters = parser.getParameters();
80 ValidParameters validParameter;
81 map<string, string>::iterator it;
83 //check to make sure all parameters are valid for command
84 for (it = parameters.begin(); it != parameters.end(); it++) {
85 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
88 vector<string> tempOutNames;
89 outputTypes["mantel"] = tempOutNames;
91 //if the user changes the input directory command factory will send this info to us in the output parameter
92 string inputDir = validParameter.validFile(parameters, "inputdir", false);
93 if (inputDir == "not found"){ inputDir = ""; }
96 it = parameters.find("phylip1");
97 //user has given a template file
98 if(it != parameters.end()){
99 path = m->hasPath(it->second);
100 //if the user has not given a path then, add inputdir. else leave path alone.
101 if (path == "") { parameters["phylip1"] = inputDir + it->second; }
104 it = parameters.find("phylip2");
105 //user has given a template file
106 if(it != parameters.end()){
107 path = m->hasPath(it->second);
108 //if the user has not given a path then, add inputdir. else leave path alone.
109 if (path == "") { parameters["phylip2"] = inputDir + it->second; }
114 //check for required parameters
115 phylipfile1 = validParameter.validFile(parameters, "phylip1", true);
116 if (phylipfile1 == "not open") { phylipfile1 = ""; abort = true; }
117 else if (phylipfile1 == "not found") { phylipfile1 = ""; m->mothurOut("phylip1 is a required parameter for the mantel command."); m->mothurOutEndLine(); abort = true; }
119 phylipfile2 = validParameter.validFile(parameters, "phylip2", true);
120 if (phylipfile2 == "not open") { phylipfile2 = ""; abort = true; }
121 else if (phylipfile2 == "not found") { phylipfile2 = ""; m->mothurOut("phylip2 is a required parameter for the mantel command."); m->mothurOutEndLine(); abort = true; }
123 outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = m->hasPath(phylipfile1); }
125 method = validParameter.validFile(parameters, "method", false); if (method == "not found"){ method = "pearson"; }
127 string temp = validParameter.validFile(parameters, "iters", false); if (temp == "not found") { temp = "1000"; }
128 convert(temp, iters);
130 if ((method != "pearson") && (method != "spearman") && (method != "kendall")) { m->mothurOut(method + " is not a valid method. Valid methods are pearson, spearman, and kendall."); m->mothurOutEndLine(); abort = true; }
133 catch(exception& e) {
134 m->errorOut(e, "MantelCommand", "MantelCommand");
138 //**********************************************************************************************************************
140 int MantelCommand::execute(){
143 if (abort == true) { if (calledHelp) { return 0; } return 2; }
145 /***************************************************/
146 // reading distance files //
147 /***************************************************/
150 ReadPhylipVector readMatrix(phylipfile1);
151 vector< vector<double> > matrix1;
152 vector<string> names1 = readMatrix.read(matrix1);
154 if (m->control_pressed) { return 0; }
157 ReadPhylipVector readMatrix2(phylipfile2);
158 vector< vector<double> > matrix2;
159 vector<string> names2 = readMatrix2.read(matrix2);
161 if (m->control_pressed) { return 0; }
163 //make sure matrix2 and matrix1 are in the same order
164 if (names1 == names2) { //then everything is in same order and same size
165 }else if (names1.size() != names2.size()) { //wrong size no need to order, abort
166 m->mothurOut("[ERROR]: distance matrices are not the same size, aborting."); m->mothurOutEndLine();
167 m->control_pressed = true;
168 }else { //sizes are the same, but either the names are different or they are in different order
169 m->mothurOut("[WARNING]: Names do not match between distance files. Comparing based on order in files."); m->mothurOutEndLine();
172 if (m->control_pressed) { return 0; }
174 /***************************************************/
175 // calculating mantel and signifigance //
176 /***************************************************/
178 //calc mantel coefficient
179 LinearAlgebra linear;
181 if (method == "pearson") { mantel = linear.calcPearson(matrix1, matrix2); }
182 else if (method == "spearman") { mantel = linear.calcSpearman(matrix1, matrix2); }
183 else if (method == "kendall") { mantel = linear.calcKendall(matrix1, matrix2); }
188 for (int i = 0; i < iters; i++) {
190 if (m->control_pressed) { return 0; }
193 vector< vector<double> > matrix2Copy = matrix2;
194 random_shuffle(matrix2Copy.begin(), matrix2Copy.end());
197 double randomMantel = 0.0;
198 if (method == "pearson") { randomMantel = linear.calcPearson(matrix1, matrix2Copy); }
199 else if (method == "spearman") { randomMantel = linear.calcSpearman(matrix1, matrix2Copy); }
200 else if (method == "kendall") { randomMantel = linear.calcKendall(matrix1, matrix2Copy); }
202 if (randomMantel >= mantel) { count++; }
205 double pValue = count / (float) iters;
207 if (m->control_pressed) { return 0; }
209 string outputFile = outputDir + m->getRootName(m->getSimpleName(phylipfile1)) + "mantel";
210 outputNames.push_back(outputFile); outputTypes["mantel"].push_back(outputFile);
213 m->openOutputFile(outputFile, out);
215 out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
216 cout.setf(ios::fixed, ios::floatfield); cout.setf(ios::showpoint);
218 out << "Mantel\tpValue" << endl;
219 out << mantel << '\t' << pValue << endl;
223 cout << "\nmantel = " << mantel << "\tpValue = " << pValue << endl;
224 m->mothurOutJustToLog("\nmantel = " + toString(mantel) + "\tpValue = " + toString(pValue) + "\n");
226 m->mothurOutEndLine();
227 m->mothurOut("Output File Names: "); m->mothurOutEndLine();
228 for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
229 m->mothurOutEndLine();
233 catch(exception& e) {
234 m->errorOut(e, "MantelCommand", "execute");
239 //**********************************************************************************************************************