]> git.donarmstrong.com Git - samtools.git/blobdiff - bam_tview.c
cont
[samtools.git] / bam_tview.c
index 5858e3b40c5999aed6ac09d91e681de4cb55a8c1..ede6b0c87f57d17ad25b5933da85cedd881f719b 100644 (file)
@@ -1,43 +1,85 @@
-#ifndef _NO_CURSES
-#include <curses.h>
-#include <ctype.h>
 #include <assert.h>
-#include <string.h>
-#include "bam.h"
-#include "faidx.h"
-#include "bam_maqcns.h"
+#include "bam_tview.h"
 
-#define TV_MIN_ALNROW 2
-#define TV_MAX_GOTO  40
-#define TV_LOW_MAPQ  10
+int base_tv_init(tview_t* tv,const char *fn, const char *fn_fa, const char *samples)
+       {
+       assert(tv!=NULL);
+       assert(fn!=NULL);
+       tv->mrow = 24; tv->mcol = 80;
+       tv->color_for = TV_COLOR_MAPQ;
+       tv->is_dot = 1;
+       
+       tv->fp = bam_open(fn, "r");
+       if(tv->fp==0)
+               {
+               fprintf(stderr,"bam_open %s. %s\n", fn,fn_fa);
+               exit(EXIT_FAILURE);
+               }
+       bgzf_set_cache_size(tv->fp, 8 * 1024 *1024);
+       assert(tv->fp);
+       
+       tv->header = bam_header_read(tv->fp);
+       if(tv->header==0)
+               {
+               fprintf(stderr,"Cannot read '%s'.\n", fn);
+               exit(EXIT_FAILURE);
+               }
+       tv->idx = bam_index_load(fn);
+       if (tv->idx == 0)
+               {
+               fprintf(stderr,"Cannot read index for '%s'.\n", fn);
+               exit(EXIT_FAILURE);
+               }
+       tv->lplbuf = bam_lplbuf_init(tv_pl_func, tv);
+       if (fn_fa) tv->fai = fai_load(fn_fa);
+       tv->bca = bcf_call_init(0.83, 13);
+       tv->ins = 1;
 
-#define TV_COLOR_MAPQ   0
-#define TV_COLOR_BASEQ  1
-#define TV_COLOR_NUCL   2
-#define TV_COLOR_COL    3
-#define TV_COLOR_COLQ   4
+    if ( samples ) 
+    {
+        if ( !tv->header->dict ) tv->header->dict = sam_header_parse2(tv->header->text);
+        void *iter = tv->header->dict;
+        const char *key, *val;
+        int n = 0;
+        tv->rg_hash = kh_init(kh_rg);
+        while ( (iter = sam_header2key_val(iter, "RG","ID","SM", &key, &val)) )
+        {
+            if ( !strcmp(samples,key) || (val && !strcmp(samples,val)) )
+            {
+                khiter_t k = kh_get(kh_rg, tv->rg_hash, key);
+                if ( k != kh_end(tv->rg_hash) ) continue;
+                int ret;
+                k = kh_put(kh_rg, tv->rg_hash, key, &ret);
+                kh_value(tv->rg_hash, k) = val;
+                n++;
+            }
+        }
+        if ( !n )
+        {
+            fprintf(stderr,"The sample or read group \"%s\" not present.\n", samples);
+            exit(EXIT_FAILURE);
+        }
+    }
 
-#define TV_BASE_NUCL 0
-#define TV_BASE_COLOR_SPACE 1
+       return 0;
+       }
 
-typedef struct {
-       int mrow, mcol;
-       WINDOW *wgoto, *whelp;
 
-       bam_index_t *idx;
-       bam_lplbuf_t *lplbuf;
-       bam_header_t *header;
-       bamFile fp;
-       int curr_tid, left_pos;
-       faidx_t *fai;
-       bam_maqcns_t *bmc;
+void base_tv_destroy(tview_t* tv)
+       {
+       bam_lplbuf_destroy(tv->lplbuf);
+       bcf_call_destroy(tv->bca);
+       bam_index_destroy(tv->idx);
+       if (tv->fai) fai_destroy(tv->fai);
+       free(tv->ref);
+       bam_header_destroy(tv->header);
+       bam_close(tv->fp);
+       }
 
-       int ccol, last_pos, row_shift, base_for, color_for, is_dot, l_ref, ins;
-       char *ref;
-} tview_t;
 
 int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void *data)
 {
+       extern unsigned char bam_nt16_table[256];
        tview_t *tv = (tview_t*)data;
        int i, j, c, rb, attr, max_ins = 0;
        uint32_t call = 0;
@@ -45,22 +87,37 @@ int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void
        // print referece
        rb = (tv->ref && pos - tv->left_pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N';
        for (i = tv->last_pos + 1; i < pos; ++i) {
-               if (i%10 == 0) mvprintw(0, tv->ccol, "%-d", i+1);
+               if (i%10 == 0 && tv->mcol - tv->ccol >= 10) tv->my_mvprintw(tv,0, tv->ccol, "%-d", i+1);
                c = tv->ref? tv->ref[i - tv->left_pos] : 'N';
-               mvaddch(1, tv->ccol++, c);
+               tv->my_mvaddch(tv,1, tv->ccol++, c);
+       }
+       if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) tv->my_mvprintw(tv,0, tv->ccol, "%-d", pos+1);
+       { // call consensus
+               bcf_callret1_t bcr;
+               int qsum[4], a1, a2, tmp;
+               double p[3], prior = 30;
+               bcf_call_glfgen(n, pl, bam_nt16_table[rb], tv->bca, &bcr);
+               for (i = 0; i < 4; ++i) qsum[i] = bcr.qsum[i]<<2 | i;
+               for (i = 1; i < 4; ++i) // insertion sort
+                       for (j = i; j > 0 && qsum[j] > qsum[j-1]; --j)
+                               tmp = qsum[j], qsum[j] = qsum[j-1], qsum[j-1] = tmp;
+               a1 = qsum[0]&3; a2 = qsum[1]&3;
+               p[0] = bcr.p[a1*5+a1]; p[1] = bcr.p[a1*5+a2] + prior; p[2] = bcr.p[a2*5+a2];
+               if ("ACGT"[a1] != toupper(rb)) p[0] += prior + 3;
+               if ("ACGT"[a2] != toupper(rb)) p[2] += prior + 3;
+               if (p[0] < p[1] && p[0] < p[2]) call = (1<<a1)<<16 | (int)((p[1]<p[2]?p[1]:p[2]) - p[0] + .499);
+               else if (p[2] < p[1] && p[2] < p[0]) call = (1<<a2)<<16 | (int)((p[0]<p[1]?p[0]:p[1]) - p[2] + .499);
+               else call = (1<<a1|1<<a2)<<16 | (int)((p[0]<p[2]?p[0]:p[2]) - p[1] + .499);
        }
-       if (pos%10 == 0) mvprintw(0, tv->ccol, "%-d", pos+1);
-       // print consensus
-       call = bam_maqcns_call(n, pl, tv->bmc);
-       attr = A_UNDERLINE;
-       c = ",ACMGRSVTWYHKDBN"[call>>28&0xf];
-       i = (call>>8&0xff)/10+1;
+       attr = tv->my_underline(tv);
+       c = ",ACMGRSVTWYHKDBN"[call>>16&0xf];
+       i = (call&0xffff)/10+1;
        if (i > 4) i = 4;
-       attr |= COLOR_PAIR(i);
+       attr |= tv->my_colorpair(tv,i);
        if (c == toupper(rb)) c = '.';
-       attron(attr);
-       mvaddch(2, tv->ccol, c);
-       attroff(attr);
+       tv->my_attron(tv,attr);
+       tv->my_mvaddch(tv,2, tv->ccol, c);
+       tv->my_attroff(tv,attr);
        if(tv->ins) {
                // calculate maximum insert
                for (i = 0; i < n; ++i) {
@@ -77,23 +134,30 @@ int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void
                                if (!p->is_del) {
                                        if (tv->base_for == TV_BASE_COLOR_SPACE && 
                                                        (c = bam_aux_getCSi(p->b, p->qpos))) {
-                                               c = bam_aux_getCSi(p->b, p->qpos);
                                                // assume that if we found one color, we will be able to get the color error
                                                if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos)) c = bam1_strand(p->b)? ',' : '.';
+                                       } else {
+                                               if (tv->show_name) {
+                                                       char *name = bam1_qname(p->b);
+                                                       c = (p->qpos + 1 >= p->b->core.l_qname)? ' ' : name[p->qpos];
+                                               } else {
+                                                       c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos)];
+                                                       if (tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.';
+                                               }
                                        }
-                                       else {
-                                               c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos)];
-                                               if (tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.';
-                                       }
-                               } else c = '*';
+                               } else c = p->is_refskip? (bam1_strand(p->b)? '<' : '>') : '*';
                        } else { // padding
                                if (j > p->indel) c = '*';
                                else { // insertion
                                        if (tv->base_for ==  TV_BASE_NUCL) {
-                                               c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos + j)];
-                                               if (j == 0 && tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.';
-                                       }
-                                       else {
+                                               if (tv->show_name) {
+                                                       char *name = bam1_qname(p->b);
+                                                       c = (p->qpos + j + 1 >= p->b->core.l_qname)? ' ' : name[p->qpos + j];
+                                               } else {
+                                                       c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos + j)];
+                                                       if (j == 0 && tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.';
+                                               }
+                                       } else {
                                                c = bam_aux_getCSi(p->b, p->qpos + j);
                                                if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos + j)) c = bam1_strand(p->b)? ',' : '.';
                                        }
@@ -103,18 +167,18 @@ int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void
                                int x;
                                attr = 0;
                                if (((p->b->core.flag&BAM_FPAIRED) && !(p->b->core.flag&BAM_FPROPER_PAIR))
-                                               || (p->b->core.flag & BAM_FSECONDARY)) attr |= A_UNDERLINE;
+                                               || (p->b->core.flag & BAM_FSECONDARY)) attr |= tv->my_underline(tv);
                                if (tv->color_for == TV_COLOR_BASEQ) {
                                        x = bam1_qual(p->b)[p->qpos]/10 + 1;
                                        if (x > 4) x = 4;
-                                       attr |= COLOR_PAIR(x);
+                                       attr |= tv->my_colorpair(tv,x);
                                } else if (tv->color_for == TV_COLOR_MAPQ) {
                                        x = p->b->core.qual/10 + 1;
                                        if (x > 4) x = 4;
-                                       attr |= COLOR_PAIR(x);
+                                       attr |= tv->my_colorpair(tv,x);
                                } else if (tv->color_for == TV_COLOR_NUCL) {
                                        x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)] + 5;
-                                       attr |= COLOR_PAIR(x);
+                                       attr |= tv->my_colorpair(tv,x);
                                } else if(tv->color_for == TV_COLOR_COL) {
                                        x = 0;
                                        switch(bam_aux_getCSi(p->b, p->qpos)) {
@@ -126,97 +190,61 @@ int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void
                                                default: x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)]; break;
                                        }
                                        x+=5;
-                                       attr |= COLOR_PAIR(x);
+                                       attr |= tv->my_colorpair(tv,x);
                                } else if(tv->color_for == TV_COLOR_COLQ) {
                                        x = bam_aux_getCQi(p->b, p->qpos);
                                        if(0 == x) x = bam1_qual(p->b)[p->qpos];
                                        x = x/10 + 1;
                                        if (x > 4) x = 4;
-                                       attr |= COLOR_PAIR(x);
+                                       attr |= tv->my_colorpair(tv,x);
                                }
-                               attron(attr);
-                               mvaddch(row, tv->ccol, bam1_strand(p->b)? tolower(c) : toupper(c));
-                               attroff(attr);
+                               tv->my_attron(tv,attr);
+                               tv->my_mvaddch(tv,row, tv->ccol, bam1_strand(p->b)? tolower(c) : toupper(c));
+                               tv->my_attroff(tv,attr);
                        }
                }
                c = j? '*' : rb;
                if (c == '*') {
-                       attr = COLOR_PAIR(8);
-                       attron(attr);
-                       mvaddch(1, tv->ccol++, c);
-                       attroff(attr);
-               } else mvaddch(1, tv->ccol++, c);
+                       attr = tv->my_colorpair(tv,8);
+                       tv->my_attron(tv,attr);
+                       tv->my_mvaddch(tv,1, tv->ccol++, c);
+                       tv->my_attroff(tv,attr);
+               } else tv->my_mvaddch(tv,1, tv->ccol++, c);
        }
        tv->last_pos = pos;
        return 0;
 }
 
-tview_t *tv_init(const char *fn, const char *fn_fa)
-{
-       tview_t *tv = (tview_t*)calloc(1, sizeof(tview_t));
-       tv->is_dot = 1;
-       tv->idx = bam_index_load(fn);
-       tv->fp = bam_open(fn, "r");
-       assert(tv->fp);
-       tv->header = bam_header_read(tv->fp);
-       tv->lplbuf = bam_lplbuf_init(tv_pl_func, tv);
-       if (fn_fa) tv->fai = fai_load(fn_fa);
-       tv->bmc = bam_maqcns_init();
-       tv->ins = 1;
-       bam_maqcns_prepare(tv->bmc);
 
-       initscr();
-       keypad(stdscr, TRUE);
-       clear();
-       noecho();
-       cbreak();
-#ifdef NCURSES_VERSION
-       getmaxyx(stdscr, tv->mrow, tv->mcol);
-#else
-       tv->mrow = 80; tv->mcol = 40;
-#endif
-       tv->wgoto = newwin(3, TV_MAX_GOTO + 10, 10, 5);
-       tv->whelp = newwin(27, 40, 5, 5);
-       tv->color_for = TV_COLOR_MAPQ;
-       start_color();
-       init_pair(1, COLOR_BLUE, COLOR_BLACK);
-       init_pair(2, COLOR_GREEN, COLOR_BLACK);
-       init_pair(3, COLOR_YELLOW, COLOR_BLACK);
-       init_pair(4, COLOR_WHITE, COLOR_BLACK);
-       init_pair(5, COLOR_GREEN, COLOR_BLACK);
-       init_pair(6, COLOR_CYAN, COLOR_BLACK);
-       init_pair(7, COLOR_YELLOW, COLOR_BLACK);
-       init_pair(8, COLOR_RED, COLOR_BLACK);
-       init_pair(9, COLOR_BLUE, COLOR_BLACK);
-       return tv;
-}
 
-void tv_destroy(tview_t *tv)
-{
-       delwin(tv->wgoto); delwin(tv->whelp);
-       endwin();
-
-       bam_lplbuf_destroy(tv->lplbuf);
-       bam_maqcns_destroy(tv->bmc);
-       bam_index_destroy(tv->idx);
-       if (tv->fai) fai_destroy(tv->fai);
-       free(tv->ref);
-       bam_header_destroy(tv->header);
-       bam_close(tv->fp);
-       free(tv);
-}
 
 int tv_fetch_func(const bam1_t *b, void *data)
 {
        tview_t *tv = (tview_t*)data;
+    if ( tv->rg_hash )
+    {
+        const uint8_t *rg = bam_aux_get(b, "RG");
+        if ( !rg ) return 0; 
+        khiter_t k = kh_get(kh_rg, tv->rg_hash, (const char*)(rg + 1));
+        if ( k == kh_end(tv->rg_hash) ) return 0;
+    }
+       if (tv->no_skip) {
+               uint32_t *cigar = bam1_cigar(b); // this is cheating...
+               int i;
+               for (i = 0; i <b->core.n_cigar; ++i) {
+                       if ((cigar[i]&0xf) == BAM_CREF_SKIP)
+                               cigar[i] = cigar[i]>>4<<4 | BAM_CDEL;
+               }
+       }
        bam_lplbuf_push(b, tv->lplbuf);
        return 0;
 }
 
-int tv_draw_aln(tview_t *tv, int tid, int pos)
-{
+int base_draw_aln(tview_t *tv, int tid, int pos)
+       {
+       assert(tv!=NULL);
        // reset
-       clear();
+       tv->my_clear(tv);
        tv->curr_tid = tid; tv->left_pos = pos;
        tv->last_pos = tv->left_pos - 1;
        tv->ccol = 0;
@@ -224,7 +252,10 @@ int tv_draw_aln(tview_t *tv, int tid, int pos)
        if (tv->fai) {
                char *str;
                if (tv->ref) free(tv->ref);
+               assert(tv->curr_tid>=0);
+               
                str = (char*)calloc(strlen(tv->header->target_name[tv->curr_tid]) + 30, 1);
+               assert(str!=NULL);
                sprintf(str, "%s:%d-%d", tv->header->target_name[tv->curr_tid], tv->left_pos + 1, tv->left_pos + tv->mcol);
                tv->ref = fai_fetch(tv->fai, str, &tv->l_ref);
                free(str);
@@ -233,132 +264,105 @@ int tv_draw_aln(tview_t *tv, int tid, int pos)
        bam_lplbuf_reset(tv->lplbuf);
        bam_fetch(tv->fp, tv->idx, tv->curr_tid, tv->left_pos, tv->left_pos + tv->mcol, tv, tv_fetch_func);
        bam_lplbuf_push(0, tv->lplbuf);
-       return 0;
-}
 
-static void tv_win_goto(tview_t *tv, int *tid, int *pos)
-{
-       char str[256];
-       int i, l = 0;
-       wborder(tv->wgoto, '|', '|', '-', '-', '+', '+', '+', '+');
-       mvwprintw(tv->wgoto, 1, 2, "Goto: ");
-       for (;;) {
-               int c = wgetch(tv->wgoto);
-               wrefresh(tv->wgoto);
-               if (c == KEY_BACKSPACE || c == '\010' || c == '\177') {
-                       --l;
-               } else if (c == KEY_ENTER || c == '\012' || c == '\015') {
-                       int _tid = -1, _beg, _end;
-                       bam_parse_region(tv->header, str, &_tid, &_beg, &_end);
-                       if (_tid >= 0) {
-                               *tid = _tid; *pos = _beg;
-                               return;
-                       }
-               } else if (isgraph(c)) {
-                       if (l < TV_MAX_GOTO) str[l++] = c;
-               } else if (c == '\027') l = 0;
-               else if (c == '\033') return;
-               str[l] = '\0';
-               for (i = 0; i < TV_MAX_GOTO; ++i) mvwaddch(tv->wgoto, 1, 8 + i, ' ');
-               mvwprintw(tv->wgoto, 1, 8, "%s", str);
+       while (tv->ccol < tv->mcol) {
+               int pos = tv->last_pos + 1;
+               if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) tv->my_mvprintw(tv,0, tv->ccol, "%-d", pos+1);
+               tv->my_mvaddch(tv,1, tv->ccol++, (tv->ref && pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N');
+               ++tv->last_pos;
        }
+       return 0;
 }
 
-static void tv_win_help(tview_t *tv) {
-       int r = 1;
-       WINDOW *win = tv->whelp;
-       wborder(win, '|', '|', '-', '-', '+', '+', '+', '+');
-       mvwprintw(win, r++, 2, "        -=-    Help    -=- ");
-       r++;
-       mvwprintw(win, r++, 2, "?          This window");
-       mvwprintw(win, r++, 2, "Arrows     Small scroll movement");
-       mvwprintw(win, r++, 2, "h,j,k,l    Small scroll movement");
-       mvwprintw(win, r++, 2, "H,J,K,L    Large scroll movement");
-       mvwprintw(win, r++, 2, "ctrl-H     Scroll 1k left");
-       mvwprintw(win, r++, 2, "ctrl-L     Scroll 1k right");
-       mvwprintw(win, r++, 2, "space      Scroll one screen");
-       mvwprintw(win, r++, 2, "backspace  Scroll back one screen");
-       mvwprintw(win, r++, 2, "g          Go to specific location");
-       mvwprintw(win, r++, 2, "m          Color for mapping qual");
-       mvwprintw(win, r++, 2, "n          Color for nucleotide");
-       mvwprintw(win, r++, 2, "b          Color for base quality");
-       mvwprintw(win, r++, 2, "c          Color for cs color");
-       mvwprintw(win, r++, 2, "z          Color for cs qual");
-       mvwprintw(win, r++, 2, ".          Toggle on/off dot view");
-       mvwprintw(win, r++, 2, "N          Turn on nt view");
-       mvwprintw(win, r++, 2, "C          Turn on cs view");
-       mvwprintw(win, r++, 2, "i          Toggle on/off ins");
-       mvwprintw(win, r++, 2, "q          Exit");
-       r++;
-       mvwprintw(win, r++, 2, "Underline:      Secondary or orphan");
-       mvwprintw(win, r++, 2, "Blue:    0-9    Green: 10-19");
-       mvwprintw(win, r++, 2, "Yellow: 20-29   White: >=30");
-       wrefresh(win);
-       wgetch(win);
-}
 
-void tv_loop(tview_t *tv)
+
+
+static void error(const char *format, ...)
 {
-       int tid, pos;
-       tid = tv->curr_tid; pos = tv->left_pos;
-       while (1) {
-               int c = getch();
-               if(256 < c) {c = 1 + (c%256);} // Terminal was displaying ctrl-H as 263 via ssh from Mac OS X 10.5 computer 
-               switch (c) {
-                       case '?': tv_win_help(tv); break;
-                       case '\033':
-                       case 'q': goto end_loop;
-                       case 'g': tv_win_goto(tv, &tid, &pos); break;
-                       case 'm': tv->color_for = TV_COLOR_MAPQ; break;
-                       case 'b': tv->color_for = TV_COLOR_BASEQ; break;
-                       case 'n': tv->color_for = TV_COLOR_NUCL; break;
-                       case 'c': tv->color_for = TV_COLOR_COL; break;
-                       case 'z': tv->color_for = TV_COLOR_COLQ; break;
-                       case KEY_LEFT:
-                       case 'h': --pos; break;
-                       case KEY_RIGHT:
-                       case 'l': ++pos; break;
-                       case KEY_SLEFT:
-                       case 'H': pos -= 20; break;
-                       case KEY_SRIGHT:
-                       case 'L': pos += 20; break;
-                       case '.': tv->is_dot = !tv->is_dot; break;
-                       case 'N': tv->base_for = TV_BASE_NUCL; break;
-                       case 'C': tv->base_for = TV_BASE_COLOR_SPACE; break;
-                       case 'i': tv->ins = !tv->ins; break;
-                       case '\010': pos -= 1000; break;
-                       case '\014': pos += 1000; break;
-                       case ' ': pos += tv->mcol; break;
-                       case KEY_UP:
-                       case 'j': --tv->row_shift; break;
-                       case KEY_DOWN:
-                       case 'k': ++tv->row_shift; break;
-                       case KEY_BACKSPACE:
-                       case '\177': pos -= tv->mcol; break;
-#ifdef KEY_RESIZE
-                       case KEY_RESIZE: getmaxyx(stdscr, tv->mrow, tv->mcol); break;
-#endif
-                       default: continue;
-               }
-               if (pos < 0) pos = 0;
-               if (tv->row_shift < 0) tv->row_shift = 0;
-               tv_draw_aln(tv, tid, pos);
-       }
-end_loop:
-       return;
+    if ( !format )
+    {
+        fprintf(stderr, "\n");
+        fprintf(stderr, "Usage: bamtk tview [options] <aln.bam> [ref.fasta]\n");
+        fprintf(stderr, "Options:\n");
+        fprintf(stderr, "   -p chr:pos      go directly to this position\n");
+        fprintf(stderr, "   -s STR          display only reads from this sample or group\n");
+        fprintf(stderr, "   -d display      (H)tml or (C)urses or (T)ext \n");
+        fprintf(stderr, "\n\n");
+    }
+    else
+    {
+        va_list ap;
+        va_start(ap, format);
+        vfprintf(stderr, format, ap);
+        va_end(ap);
+    }
+    exit(-1);
 }
 
+enum dipsay_mode {display_ncurses,display_html,display_text};
+extern tview_t* curses_tv_init(const char *fn, const char *fn_fa, const char *samples);
+extern tview_t* html_tv_init(const char *fn, const char *fn_fa, const char *samples);
+extern tview_t* text_tv_init(const char *fn, const char *fn_fa, const char *samples);
+
 int bam_tview_main(int argc, char *argv[])
-{
-       tview_t *tv;
-       if (argc == 1) {
-               fprintf(stderr, "Usage: bamtk tview <aln.bam> [ref.fasta]\n");
-               return 1;
+       {
+       int view_mode=display_ncurses;
+       tview_t* tv=NULL;
+    char *samples=NULL, *position=NULL;
+    int c;
+    while ((c = getopt(argc, argv, "s:p:d:")) >= 0) {
+        switch (c) {
+            case 's': samples=optarg; break;
+            case 'p': position=optarg; break;
+            case 'd':
+               {
+               switch(optarg[0])
+                       {
+                       case 'H': case 'h': view_mode=display_html;break;
+                       case 'T': case 't': view_mode=display_text;break;
+                       case 'C': case 'c': view_mode=display_ncurses;break;
+                       default: view_mode=display_ncurses;break;
+                       }
+               break;
+               }
+            default: error(NULL);
+        }
+    }
+       if (argc==optind) error(NULL);
+       
+       switch(view_mode)
+               {
+               case display_ncurses:
+                       {
+                       tv = curses_tv_init(argv[optind], (optind+1>=argc)? 0 : argv[optind+1], samples);
+                       break;
+                       }
+               case display_text:
+                       {
+                       tv = text_tv_init(argv[optind], (optind+1>=argc)? 0 : argv[optind+1], samples);
+                       break;
+                       }
+               case display_html:
+                       {
+                       tv = html_tv_init(argv[optind], (optind+1>=argc)? 0 : argv[optind+1], samples);
+                       break;
+                       }
+               }
+       if(tv==NULL)
+               {
+               error("cannot create view");
+               return EXIT_FAILURE;
+               }
+       
+       if ( position )
+                {
+               int _tid = -1, _beg, _end;
+               bam_parse_region(tv->header, position, &_tid, &_beg, &_end);
+               if (_tid >= 0) { tv->curr_tid = _tid; tv->left_pos = _beg; }
+               }
+       tv->my_drawaln(tv, tv->curr_tid, tv->left_pos);
+       tv->my_loop(tv);
+       tv->my_destroy(tv);
+       
+       return EXIT_SUCCESS;
        }
-       tv = tv_init(argv[1], (argc == 2)? 0 : argv[2]);
-       tv_draw_aln(tv, 0, 0);
-       tv_loop(tv);
-       tv_destroy(tv);
-       return 0;
-}
-#endif