* samtools-0.1.9-1 (r786)

[samtools.git] / bam2bcf.c

diff --git a/bam2bcf.c b/bam2bcf.c
index e55212ca98cbb28041fb6a6dfc46619ff595ee09..509761d3395df7d01e56f7a368f2f2daa29be4b3 100644 (file)
--- a/bam2bcf.c
+++ b/bam2bcf.c
@@ -154,8 +154,9 @@ int bcf_call_combine(int n, const bcf_callret1_t *calls, int ref_base /*4-bit*/,
        return 0;
 }
 
-int bcf_call2bcf(int tid, int pos, bcf_call_t *bc, bcf1_t *b)
+int bcf_call2bcf(int tid, int pos, bcf_call_t *bc, bcf1_t *b, bcf_callret1_t *bcr, int is_SP)
 {
+       extern double kt_fisher_exact(int n11, int n12, int n21, int n22, double *_left, double *_right, double *two);
        kstring_t s;
        int i;
        b->n_smpl = bc->n;
@@ -178,9 +179,36 @@ int bcf_call2bcf(int tid, int pos, bcf_call_t *bc, bcf1_t *b)
        }
        kputc('\0', &s);
        // FMT
-       kputs("PL", &s); kputc('\0', &s);
+       kputs("PL", &s);
+       if (bcr) {
+               kputs(":DP", &s);
+               if (is_SP) kputs(":SP", &s);
+       }
+       kputc('\0', &s);
        b->m_str = s.m; b->str = s.s; b->l_str = s.l;
        bcf_sync(b);
        memcpy(b->gi[0].data, bc->PL, b->gi[0].len * bc->n);
+       if (bcr) {
+               uint16_t *dp = (uint16_t*)b->gi[1].data;
+               uint8_t *sp = is_SP? b->gi[2].data : 0;
+               for (i = 0; i < bc->n; ++i) {
+                       bcf_callret1_t *p = bcr + i;
+                       dp[i] = p->depth < 0xffff? p->depth : 0xffff;
+                       if (is_SP) {
+                               if (p->anno[0] + p->anno[1] < 2 || p->anno[2] + p->anno[3] < 2
+                                       || p->anno[0] + p->anno[2] < 2 || p->anno[1] + p->anno[3] < 2)
+                               {
+                                       sp[i] = 0;
+                               } else {
+                                       double left, right, two;
+                                       int x;
+                                       kt_fisher_exact(p->anno[0], p->anno[1], p->anno[2], p->anno[3], &left, &right, &two);
+                                       x = (int)(-4.343 * log(two) + .499);
+                                       if (x > 255) x = 255;
+                                       sp[i] = x;
+                               }
+                       }
+               }
+       }
        return 0;
 }