X-Git-Url: https://git.donarmstrong.com/?p=rsem.git;a=blobdiff_plain;f=SingleRead.h;h=8ea4eec510e506ea9e1728e21766d96969e2b308;hp=2531176b6cb470941537dce24fb58e26eb0967d8;hb=b64d62d49f9b0446f10f87a2aadcde2f36854ab6;hpb=a95154919f950f86de9104b2b9dcf1f0c7e83387

diff --git a/SingleRead.h b/SingleRead.h
index 2531176..8ea4eec 100644
--- a/SingleRead.h
+++ b/SingleRead.h
@@ -12,75 +12,81 @@
 #include "Read.h"
 
 class SingleRead : public Read {
- public:
-  SingleRead() { readseq = ""; len = 0; }
-  SingleRead(const std::string& name, const std::string& readseq) {
-	  this->name = name;
-	  this->readseq = readseq;
-	  this->len = readseq.length();
-	  calc_lq();
-  }
-
-  bool read(int argc, std::istream* argv[], int flags = 7);
-  void write(int argc, std::ostream* argv[]);
-
-  const int getReadLength() const { return len; /*readseq.length();*/ } // If need memory and .length() are guaranteed O(1), use statement in /* */
-  const std::string& getReadSeq() const { return readseq; }
-
- private:
-  int len; // read length
-  std::string readseq; // read sequence
-
-  void calc_lq();
+public:
+	SingleRead() { readseq = ""; len = 0; }
+	SingleRead(const std::string& name, const std::string& readseq) {
+		this->name = name;
+		this->readseq = readseq;
+		this->len = readseq.length();
+	}
+
+	bool read(int argc, std::istream* argv[], int flags = 7);
+	void write(int argc, std::ostream* argv[]);
+
+	const int getReadLength() const { return len; /*readseq.length();*/ } // If need memory and .length() are guaranteed O(1), use statement in /* */
+	const std::string& getReadSeq() const { return readseq; }
+
+	void calc_lq(bool, int); // calculate if this read is low quality. Without calling this function, isLowQuality() will always be false
+
+private:
+	int len; // read length
+	std::string readseq; // read sequence
 };
 
 //If return false, you should not trust the value of any member
 bool SingleRead::read(int argc, std::istream* argv[], int flags) {
-  std::string line;
-
-  assert(argc == 1);
-  if (!getline((*argv[0]), line)) return false;
-  if (line[0] != '>') { fprintf(stderr, "Read file does not look like a FASTA file!"); exit(-1); }
-  name = "";
-  if (flags & 4) { name = line.substr(1); }
-  if (!getline((*argv[0]), readseq)) return false;
-  len = readseq.length(); // set read length
-  if (!(flags & 1)) { readseq = ""; }
+	std::string line;
 
-  if (flags & 1) calc_lq();
+	assert(argc == 1);
+	if (!getline((*argv[0]), line)) return false;
+	if (line[0] != '>') { fprintf(stderr, "Read file does not look like a FASTA file!"); exit(-1); }
+	name = "";
+	if (flags & 4) { name = line.substr(1); }
+	if (!getline((*argv[0]), readseq)) return false;
+	len = readseq.length(); // set read length
+	if (!(flags & 1)) { readseq = ""; }
 
-  return true;
+	return true;
 }
 
 void SingleRead::write(int argc, std::ostream* argv[]) {
-  assert(argc == 1);
-  (*argv[0])<<">"<<name<<std::endl<<readseq<<std::endl;
+	assert(argc == 1);
+	(*argv[0])<<">"<<name<<std::endl<<readseq<<std::endl;
 }
 
-void SingleRead::calc_lq() {
-  int numA = 0, numT = 0, numAO = 0, numTO = 0; // numAO : number of A in overlap seed region
-  int threshold_1, threshold_2;
-
-  threshold_1 = int(0.9 * len - 1.5 * sqrt(len * 1.0) + 0.5);
-  threshold_2 = (OLEN - 1) / 2 + 1;
-  for (int i = 0; i < len; i++) {
-    if (readseq[i] == 'A') { 
-      ++numA;
-      if (i < OLEN) ++numAO;
-    }
-    if (readseq[i] == 'T') {
-      ++numT;
-      if (i >= len - OLEN) ++numTO; 
-    }
-  }
+//calculate if this read is low quality
+void SingleRead::calc_lq(bool hasPolyA, int seedLen) {
+	low_quality = false;
+	if (len < seedLen) { low_quality = true; return; }
+
+	// if no polyA, no need to do the following calculation
+	if (!hasPolyA) return;
+
+	assert(readseq != "");
+
+	int numA = 0, numT = 0, numAO = 0, numTO = 0; // numAO : number of A in overlap seed region
+	int threshold_1, threshold_2;
+
+	threshold_1 = int(0.9 * len - 1.5 * sqrt(len * 1.0) + 0.5);
+	threshold_2 = (OLEN - 1) / 2 + 1;
+	for (int i = 0; i < len; i++) {
+		if (readseq[i] == 'A') {
+			++numA;
+			if (i < OLEN) ++numAO;
+		}
+		if (readseq[i] == 'T') {
+			++numT;
+			if (i >= len - OLEN) ++numTO;
+		}
+	}
   
-  if (numA >= threshold_1) {
-    low_quality = (numAO >= threshold_2);
-  }
-  else if (numT >= threshold_1) {
-    low_quality = (numTO >= threshold_2);
-  }
-  else low_quality = false;
+	if (numA >= threshold_1) {
+		low_quality = (numAO >= threshold_2);
+	}
+	else if (numT >= threshold_1) {
+		low_quality = (numTO >= threshold_2);
+	}
+	else low_quality = false;
 }
 
 #endif