X-Git-Url: https://git.donarmstrong.com/?p=rsem.git;a=blobdiff_plain;f=README.md;fp=README.md;h=4184f5d44caf4d94ebb8c5445f25d531517931d8;hp=902e806821e6de5ef39eba708edf9d43c82c69ee;hb=b64d62d49f9b0446f10f87a2aadcde2f36854ab6;hpb=7cd7abcad92a44bbd5d8d1b2bb3a871cd479c0bf diff --git a/README.md b/README.md index 902e806..4184f5d 100644 --- a/README.md +++ b/README.md @@ -56,6 +56,11 @@ To compile EBSeq, which is included in the RSEM package, run To install, simply put the rsem directory in your environment's PATH variable. +If you prefer to put all RSEM executables to a bin directory, please +also remember to put 'rsem_perl_utils.pm' and 'WHAT_IS_NEW' to the +same bin directory. 'rsem_perl_utils.pm' is required for most RSEM's +perl scripts and 'WHAT_IS_NEW' contains the RSEM version information. + ### Prerequisites C++, Perl and R are required to be installed. @@ -209,7 +214,7 @@ Here are some guidance for visualizing transcript coordinate files using IGV: 1) Import the transcript sequences as a genome -Select File -> Import Genome, then fill in ID, Name and Fasta file. Fasta file should be 'reference_name.transcripts.fa'. After that, click Save button. Suppose ID is filled as 'reference_name', a file called 'reference_name.genome' will be generated. Next time, we can use: File -> Load Genome, then select 'reference_name.genome'. +Select File -> Import Genome, then fill in ID, Name and Fasta file. Fasta file should be 'reference_name.idx.fa'. After that, click Save button. Suppose ID is filled as 'reference_name', a file called 'reference_name.genome' will be generated. Next time, we can use: File -> Load Genome, then select 'reference_name.genome'. 2) Load visualization files