0 * 0: paml format (mc.paml); 1:paup format (mc.nex) 13147 * random number seed (odd number) 5 10000 5 * <# seqs> <# sites> <# replicates> -1 * (((Human:0.06135, Chimpanzee:0.07636):0.03287, Gorilla:0.08197):0.11219, Orangutan:0.28339, Gibbon:0.42389); .5 8 * <#categories for discrete gamma> 2 mtmam.dat * [aa substitution rate file, need only if model=2 or 3] 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 A R N D C Q E G H I L K M F P S T W Y V // end of file ============================================================================= Notes for using the option in evolver to simulate amino acid sequences. Change values of parameters, but do not delete them. It is o.k. to add empty lines, but do not break down the same line into two or more lines. model = 0 (poisson), 1 (proportional), 2 (empirical), 3 (empirical_F) Use 0 for alpha to have the same rate for all sites. Use 0 for <#categories for discrete gamma> to use the continuous gamma can be dayhoff.dat, jones.dat, and so on. have to be in the right order, as indicated. =================!! Check screen output carefully!! =====================