X-Git-Url: https://git.donarmstrong.com/?p=mothur.git;a=blobdiff_plain;f=getseqscommand.cpp;h=7f0f3ddb51da8496c292367b5dfc728b3915d53f;hp=315417a30586d290b2a692e04b2e0afacb1e6fb3;hb=499f4ac6e321f9f03d4c3aa25c3b6880892c8b83;hpb=648ec37228eb16075ace911dd5a5773cdfe683da diff --git a/getseqscommand.cpp b/getseqscommand.cpp index 315417a..7f0f3dd 100644 --- a/getseqscommand.cpp +++ b/getseqscommand.cpp @@ -9,290 +9,939 @@ #include "getseqscommand.h" #include "sequence.hpp" +#include "listvector.hpp" +#include "counttable.h" //********************************************************************************************************************** +vector GetSeqsCommand::setParameters(){ + try { + CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "FNGLT", "none","fasta",false,false,true); parameters.push_back(pfasta); + CommandParameter pname("name", "InputTypes", "", "", "NameCount", "FNGLT", "none","name",false,false,true); parameters.push_back(pname); + CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup", "FNGLT", "none","count",false,false,true); parameters.push_back(pcount); + CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup", "FNGLT", "none","group",false,false,true); parameters.push_back(pgroup); + CommandParameter plist("list", "InputTypes", "", "", "none", "FNGLT", "none","list",false,false,true); parameters.push_back(plist); + CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "FNGLT", "none","taxonomy",false,false,true); parameters.push_back(ptaxonomy); + CommandParameter palignreport("alignreport", "InputTypes", "", "", "none", "FNGLT", "none","alignreport",false,false); parameters.push_back(palignreport); + CommandParameter pqfile("qfile", "InputTypes", "", "", "none", "FNGLT", "none","qfile",false,false); parameters.push_back(pqfile); + CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(paccnos); + CommandParameter pdups("dups", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pdups); + CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir); + CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir); + CommandParameter paccnos2("accnos2", "InputTypes", "", "", "none", "none", "none","",false,false); parameters.push_back(paccnos2); -GetSeqsCommand::GetSeqsCommand(string option){ + vector myArray; + for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); } + return myArray; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "setParameters"); + exit(1); + } +} +//********************************************************************************************************************** +string GetSeqsCommand::getHelpString(){ try { - abort = false; - + string helpString = ""; + helpString += "The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, count, list, taxonomy, quality or alignreport file.\n"; + helpString += "It outputs a file containing only the sequences in the .accnos file.\n"; + helpString += "The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, qfile, alignreport and dups. You must provide accnos unless you have a valid current accnos file, and at least one of the other parameters.\n"; + helpString += "The dups parameter allows you to add the entire line from a name file if you add any name from the line. default=true. \n"; + helpString += "The get.seqs command should be in the following format: get.seqs(accnos=yourAccnos, fasta=yourFasta).\n"; + helpString += "Example get.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n"; + helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n"; + return helpString; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "getHelpString"); + exit(1); + } +} + +//********************************************************************************************************************** +GetSeqsCommand::GetSeqsCommand(){ + try { + abort = true; calledHelp = true; + setParameters(); + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["taxonomy"] = tempOutNames; + outputTypes["name"] = tempOutNames; + outputTypes["group"] = tempOutNames; + outputTypes["alignreport"] = tempOutNames; + outputTypes["list"] = tempOutNames; + outputTypes["qfile"] = tempOutNames; + outputTypes["count"] = tempOutNames; + outputTypes["accnosreport"] = tempOutNames; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "GetSeqsCommand"); + exit(1); + } +} +//********************************************************************************************************************** +string GetSeqsCommand::getOutputPattern(string type) { + try { + string pattern = ""; + + if (type == "fasta") { pattern = "[filename],pick,[extension]"; } + else if (type == "taxonomy") { pattern = "[filename],pick,[extension]"; } + else if (type == "name") { pattern = "[filename],pick,[extension]"; } + else if (type == "group") { pattern = "[filename],pick,[extension]"; } + else if (type == "count") { pattern = "[filename],pick,[extension]"; } + else if (type == "list") { pattern = "[filename],[distance],pick,[extension]"; } + else if (type == "qfile") { pattern = "[filename],pick,[extension]"; } + else if (type == "accnosreport") { pattern = "[filename],pick.accnos.report"; } + else if (type == "alignreport") { pattern = "[filename],pick.align.report"; } + else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; } + + return pattern; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "getOutputPattern"); + exit(1); + } +} +//********************************************************************************************************************** +GetSeqsCommand::GetSeqsCommand(string option) { + try { + abort = false; calledHelp = false; + //allow user to run help - if(option == "help") { help(); abort = true; } + if(option == "help") { help(); abort = true; calledHelp = true; } + else if(option == "citation") { citation(); abort = true; calledHelp = true;} else { - //valid paramters for this command - string Array[] = {"fasta","name", "group", "alignreport", "accnos" }; - vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + vector myArray = setParameters(); OptionParser parser(option); map parameters = parser.getParameters(); ValidParameters validParameter; + map::iterator it; //check to make sure all parameters are valid for command - for (map::iterator it = parameters.begin(); it != parameters.end(); it++) { + for (it = parameters.begin(); it != parameters.end(); it++) { if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; } } + //initialize outputTypes + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["taxonomy"] = tempOutNames; + outputTypes["name"] = tempOutNames; + outputTypes["group"] = tempOutNames; + outputTypes["alignreport"] = tempOutNames; + outputTypes["list"] = tempOutNames; + outputTypes["qfile"] = tempOutNames; + outputTypes["accnosreport"] = tempOutNames; + outputTypes["count"] = tempOutNames; + + //if the user changes the output directory command factory will send this info to us in the output parameter + outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; } + + //if the user changes the input directory command factory will send this info to us in the output parameter + string inputDir = validParameter.validFile(parameters, "inputdir", false); + if (inputDir == "not found"){ inputDir = ""; } + else { + string path; + it = parameters.find("alignreport"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["alignreport"] = inputDir + it->second; } + } + + it = parameters.find("fasta"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["fasta"] = inputDir + it->second; } + } + + it = parameters.find("accnos"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["accnos"] = inputDir + it->second; } + } + + it = parameters.find("accnos2"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["accnos2"] = inputDir + it->second; } + } + + it = parameters.find("list"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["list"] = inputDir + it->second; } + } + + it = parameters.find("name"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["name"] = inputDir + it->second; } + } + + it = parameters.find("group"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["group"] = inputDir + it->second; } + } + + it = parameters.find("taxonomy"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["taxonomy"] = inputDir + it->second; } + } + + it = parameters.find("qfile"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["qfile"] = inputDir + it->second; } + } + + it = parameters.find("count"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["count"] = inputDir + it->second; } + } + } + + //check for required parameters accnosfile = validParameter.validFile(parameters, "accnos", true); if (accnosfile == "not open") { abort = true; } - else if (accnosfile == "not found") { accnosfile = ""; mothurOut("You must provide an accnos file."); mothurOutEndLine(); abort = true; } + else if (accnosfile == "not found") { + accnosfile = m->getAccnosFile(); + if (accnosfile != "") { m->mothurOut("Using " + accnosfile + " as input file for the accnos parameter."); m->mothurOutEndLine(); } + else { + m->mothurOut("You have no valid accnos file and accnos is required."); m->mothurOutEndLine(); + abort = true; + } + }else { m->setAccnosFile(accnosfile); } + + if (accnosfile2 == "not found") { accnosfile2 = ""; } fastafile = validParameter.validFile(parameters, "fasta", true); - if (fastafile == "not open") { abort = true; } - else if (fastafile == "not found") { fastafile = ""; } + if (fastafile == "not open") { fastafile = ""; abort = true; } + else if (fastafile == "not found") { fastafile = ""; } + else { m->setFastaFile(fastafile); } namefile = validParameter.validFile(parameters, "name", true); - if (namefile == "not open") { abort = true; } + if (namefile == "not open") { namefile = ""; abort = true; } else if (namefile == "not found") { namefile = ""; } + else { m->setNameFile(namefile); } groupfile = validParameter.validFile(parameters, "group", true); if (groupfile == "not open") { abort = true; } else if (groupfile == "not found") { groupfile = ""; } + else { m->setGroupFile(groupfile); } alignfile = validParameter.validFile(parameters, "alignreport", true); if (alignfile == "not open") { abort = true; } else if (alignfile == "not found") { alignfile = ""; } - if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "")) { mothurOut("You must provide one of the following: fasta, name, group, alignreport."); mothurOutEndLine(); abort = true; } + listfile = validParameter.validFile(parameters, "list", true); + if (listfile == "not open") { abort = true; } + else if (listfile == "not found") { listfile = ""; } + else { m->setListFile(listfile); } - if (parameters.size() > 2) { mothurOut("You may only enter one of the following: fasta, name, group, alignreport."); mothurOutEndLine(); abort = true; } - } + taxfile = validParameter.validFile(parameters, "taxonomy", true); + if (taxfile == "not open") { taxfile = ""; abort = true; } + else if (taxfile == "not found") { taxfile = ""; } + else { m->setTaxonomyFile(taxfile); } + + qualfile = validParameter.validFile(parameters, "qfile", true); + if (qualfile == "not open") { abort = true; } + else if (qualfile == "not found") { qualfile = ""; } + else { m->setQualFile(qualfile); } + + accnosfile2 = validParameter.validFile(parameters, "accnos2", true); + if (accnosfile2 == "not open") { abort = true; } + else if (accnosfile2 == "not found") { accnosfile2 = ""; } + + countfile = validParameter.validFile(parameters, "count", true); + if (countfile == "not open") { countfile = ""; abort = true; } + else if (countfile == "not found") { countfile = ""; } + else { m->setCountTableFile(countfile); } + + if ((namefile != "") && (countfile != "")) { + m->mothurOut("[ERROR]: you may only use one of the following: name or count."); m->mothurOutEndLine(); abort = true; + } + + if ((groupfile != "") && (countfile != "")) { + m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true; + } - } - catch(exception& e) { - errorOut(e, "GetSeqsCommand", "GetSeqsCommand"); - exit(1); - } -} -//********************************************************************************************************************** + + string usedDups = "true"; + string temp = validParameter.validFile(parameters, "dups", false); if (temp == "not found") { temp = "true"; usedDups = ""; } + dups = m->isTrue(temp); + + if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "") && (accnosfile2 == "") && (countfile == "")) { m->mothurOut("You must provide one of the following: fasta, name, group, count, alignreport, taxonomy, quality or listfile."); m->mothurOutEndLine(); abort = true; } + + if (countfile == "") { + if ((namefile == "") && ((fastafile != "") || (taxfile != ""))){ + vector files; files.push_back(fastafile); files.push_back(taxfile); + parser.getNameFile(files); + } + } + } -void GetSeqsCommand::help(){ - try { - mothurOut("The get.seqs command reads an .accnos file and one of the following file types: fasta, name, group or alignreport file.\n"); - mothurOut("It outputs a file containing only the sequences in the .accnos file.\n"); - mothurOut("The get.seqs command parameters are accnos, fasta, name, group and alignreport. You must provide accnos and one of the other parameters.\n"); - mothurOut("The get.seqs command should be in the following format: get.seqs(accnos=yourAccnos, fasta=yourFasta).\n"); - mothurOut("Example get.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n"); - mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n"); } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "help"); + m->errorOut(e, "GetSeqsCommand", "GetSeqsCommand"); exit(1); } } - //********************************************************************************************************************** int GetSeqsCommand::execute(){ try { - if (abort == true) { return 0; } + if (abort == true) { if (calledHelp) { return 0; } return 2; } //get names you want to keep - readAccnos(); + names = m->readAccnos(accnosfile); + + if (m->control_pressed) { return 0; } + + if (countfile != "") { + if ((fastafile != "") || (listfile != "") || (taxfile != "")) { + m->mothurOut("\n[NOTE]: The count file should contain only unique names, so mothur assumes your fasta, list and taxonomy files also contain only uniques.\n\n"); + } + } //read through the correct file and output lines you want to keep - if (fastafile != "") { readFasta(); } - else if (namefile != "") { readName(); } - else if (groupfile != "") { readGroup(); } - else if (alignfile != "") { readAlign(); } + if (namefile != "") { readName(); } + if (fastafile != "") { readFasta(); } + if (groupfile != "") { readGroup(); } + if (countfile != "") { readCount(); } + if (alignfile != "") { readAlign(); } + if (listfile != "") { readList(); } + if (taxfile != "") { readTax(); } + if (qualfile != "") { readQual(); } + if (accnosfile2 != "") { compareAccnos(); } + + if (m->debug) { runSanityCheck(); } + + if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; } + + + if (outputNames.size() != 0) { + m->mothurOutEndLine(); + m->mothurOut("Output File Names: "); m->mothurOutEndLine(); + for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); } + m->mothurOutEndLine(); + + //set fasta file as new current fastafile + string current = ""; + itTypes = outputTypes.find("fasta"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); } + } + + itTypes = outputTypes.find("name"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setNameFile(current); } + } + + itTypes = outputTypes.find("group"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setGroupFile(current); } + } + + itTypes = outputTypes.find("list"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setListFile(current); } + } + + itTypes = outputTypes.find("taxonomy"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTaxonomyFile(current); } + } + + itTypes = outputTypes.find("qfile"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); } + } + + itTypes = outputTypes.find("count"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setCountTableFile(current); } + } + } return 0; } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "execute"); + m->errorOut(e, "GetSeqsCommand", "execute"); exit(1); } } //********************************************************************************************************************** -void GetSeqsCommand::readFasta(){ +int GetSeqsCommand::readFasta(){ try { - string outputFileName = getRootName(fastafile) + "pick" + getExtension(fastafile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)); + variables["[extension]"] = m->getExtension(fastafile); + string outputFileName = getOutputFileName("fasta", variables); ofstream out; - openOutputFile(outputFileName, out); + m->openOutputFile(outputFileName, out); + ifstream in; - openInputFile(fastafile, in); + m->openInputFile(fastafile, in); string name; bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["fasta"] = temp; } while(!in.eof()){ + + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } + Sequence currSeq(in); name = currSeq.getName(); + + if (!dups) {//adjust name if needed + map::iterator it = uniqueMap.find(name); + if (it != uniqueMap.end()) { currSeq.setName(it->second); } + } + name = currSeq.getName(); + if (name != "") { //if this name is in the accnos file - if (names.count(name) == 1) { + if (names.count(name) != 0) { wroteSomething = true; currSeq.printSequence(out); - - names.erase(name); + selectedCount++; + + if (m->debug) { sanity["fasta"].insert(name); } } } - gobble(in); + m->gobble(in); } in.close(); out.close(); - if (wroteSomething == false) { - mothurOut("Your file does not contain any sequence from the .accnos file."); mothurOutEndLine(); - remove(outputFileName.c_str()); + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["fasta"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your fasta file."); m->mothurOutEndLine(); + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "readFasta"); + exit(1); + } +} +//********************************************************************************************************************** +int GetSeqsCommand::readQual(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(qualfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(qualfile)); + variables["[extension]"] = m->getExtension(qualfile); + string outputFileName = getOutputFileName("qfile", variables); + ofstream out; + m->openOutputFile(outputFileName, out); + + + ifstream in; + m->openInputFile(qualfile, in); + string name; + + bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["qual"] = temp; } + + while(!in.eof()){ + string saveName = ""; + string name = ""; + string scores = ""; + + in >> name; + + if (!dups) {//adjust name if needed + map::iterator it = uniqueMap.find(name); + if (it != uniqueMap.end()) { name = it->second; } + } + + if (name.length() != 0) { + saveName = name.substr(1); + while (!in.eof()) { + char c = in.get(); + if (c == 10 || c == 13 || c == -1){ break; } + else { name += c; } + } + m->gobble(in); + } + + while(in){ + char letter= in.get(); + if(letter == '>'){ in.putback(letter); break; } + else{ scores += letter; } + } + + m->gobble(in); + + if (names.count(saveName) != 0) { + wroteSomething = true; + + out << name << endl << scores; + selectedCount++; + if (m->debug) { sanity["qual"].insert(name); } + } + + m->gobble(in); } + in.close(); + out.close(); + + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["qfile"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your quality file."); m->mothurOutEndLine(); + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "readQual"); + exit(1); + } +} +//********************************************************************************************************************** +int GetSeqsCommand::readCount(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(countfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile)); + variables["[extension]"] = m->getExtension(countfile); + string outputFileName = getOutputFileName("count", variables); + + ofstream out; + m->openOutputFile(outputFileName, out); + + ifstream in; + m->openInputFile(countfile, in); + + bool wroteSomething = false; + int selectedCount = 0; + + string headers = m->getline(in); m->gobble(in); + out << headers << endl; + + string name, rest; int thisTotal; + while (!in.eof()) { + + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } + + in >> name; m->gobble(in); + in >> thisTotal; m->gobble(in); + rest = m->getline(in); m->gobble(in); + if (m->debug) { m->mothurOut("[DEBUG]: " + name + '\t' + rest + "\n"); } + + if (names.count(name) != 0) { + out << name << '\t' << thisTotal << '\t' << rest << endl; + wroteSomething = true; + selectedCount+= thisTotal; + } + } + in.close(); + out.close(); + + //check for groups that have been eliminated + CountTable ct; + if (ct.testGroups(outputFileName)) { + ct.readTable(outputFileName, true, false); + ct.printTable(outputFileName); + } + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your count file."); m->mothurOutEndLine(); + + return 0; } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "readFasta"); + m->errorOut(e, "GetSeqsCommand", "readCount"); exit(1); } } //********************************************************************************************************************** -void GetSeqsCommand::readName(){ +int GetSeqsCommand::readList(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(listfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(listfile)); + variables["[extension]"] = m->getExtension(listfile); + + ifstream in; + m->openInputFile(listfile, in); + + bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["list"] = temp; } + + while(!in.eof()){ + + selectedCount = 0; + + //read in list vector + ListVector list(in); + + //make a new list vector + ListVector newList; + newList.setLabel(list.getLabel()); + + variables["[distance]"] = list.getLabel(); + string outputFileName = getOutputFileName("list", variables); + + ofstream out; + m->openOutputFile(outputFileName, out); + outputTypes["list"].push_back(outputFileName); outputNames.push_back(outputFileName); + + vector binLabels = list.getLabels(); + vector newBinLabels; + + if (m->control_pressed) { in.close(); out.close(); return 0; } + + //for each bin + for (int i = 0; i < list.getNumBins(); i++) { + + //parse out names that are in accnos file + string binnames = list.get(i); + vector bnames; + m->splitAtComma(binnames, bnames); + + string newNames = ""; + for (int i = 0; i < bnames.size(); i++) { + string name = bnames[i]; + //if that name is in the .accnos file, add it + if (names.count(name) != 0) { newNames += name + ","; selectedCount++; if (m->debug) { sanity["list"].insert(name); } } + } + + //if there are names in this bin add to new list + if (newNames != "") { + newNames = newNames.substr(0, newNames.length()-1); //rip off extra comma + newList.push_back(newNames); + newBinLabels.push_back(binLabels[i]); + } + } + + //print new listvector + if (newList.getNumBins() != 0) { + wroteSomething = true; + newList.setLabels(newBinLabels); + newList.printHeaders(out); + newList.print(out); + } + + m->gobble(in); + out.close(); + } + in.close(); + + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your list file."); m->mothurOutEndLine(); + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "readList"); + exit(1); + } +} +//********************************************************************************************************************** +int GetSeqsCommand::readName(){ try { - - string outputFileName = getRootName(namefile) + "pick" + getExtension(namefile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(namefile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(namefile)); + variables["[extension]"] = m->getExtension(namefile); + string outputFileName = getOutputFileName("name", variables); ofstream out; - openOutputFile(outputFileName, out); + m->openOutputFile(outputFileName, out); + ifstream in; - openInputFile(namefile, in); + m->openInputFile(namefile, in); string name, firstCol, secondCol; bool wroteSomething = false; - + int selectedCount = 0; + + if (m->debug) { set temp; sanity["name"] = temp; } + if (m->debug) { set temp; sanity["dupname"] = temp; } while(!in.eof()){ + + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } - in >> firstCol; - in >> secondCol; + in >> firstCol; m->gobble(in); + in >> secondCol; - vector parsedNames; - //parse second column saving each name - while (secondCol.find_first_of(',') != -1) { - name = secondCol.substr(0,secondCol.find_first_of(',')); - secondCol = secondCol.substr(secondCol.find_first_of(',')+1, secondCol.length()); - parsedNames.push_back(name); - } + string hold = ""; + if (dups) { hold = secondCol; } - //get name after last , - parsedNames.push_back(secondCol); + vector parsedNames; + m->splitAtComma(secondCol, parsedNames); vector validSecond; for (int i = 0; i < parsedNames.size(); i++) { - if (names.count(parsedNames[i]) == 1) { + if (names.count(parsedNames[i]) != 0) { validSecond.push_back(parsedNames[i]); + if (m->debug) { sanity["dupname"].insert(parsedNames[i]); } } } - - //if the name in the first column is in the set then print it and any other names in second column also in set - if (names.count(firstCol) == 1) { - + if ((dups) && (validSecond.size() != 0)) { //dups = true and we want to add someone, then add everyone + for (int i = 0; i < parsedNames.size(); i++) { names.insert(parsedNames[i]); if (m->debug) { sanity["dupname"].insert(parsedNames[i]); } } + out << firstCol << '\t' << hold << endl; wroteSomething = true; - - out << firstCol << '\t'; - - //you know you have at least one valid second since first column is valid - for (int i = 0; i < validSecond.size()-1; i++) { out << validSecond[i] << ','; } - out << validSecond[validSecond.size()-1] << endl; - - - //make first name in set you come to first column and then add the remaining names to second column + selectedCount += parsedNames.size(); + if (m->debug) { sanity["name"].insert(firstCol); } }else { - //you want part of this row - if (validSecond.size() != 0) { + + selectedCount += validSecond.size(); + + //if the name in the first column is in the set then print it and any other names in second column also in set + if (names.count(firstCol) != 0) { wroteSomething = true; - out << validSecond[0] << '\t'; - + out << firstCol << '\t'; + //you know you have at least one valid second since first column is valid for (int i = 0; i < validSecond.size()-1; i++) { out << validSecond[i] << ','; } out << validSecond[validSecond.size()-1] << endl; + + if (m->debug) { sanity["name"].insert(firstCol); } + + + //make first name in set you come to first column and then add the remaining names to second column + }else { + + //you want part of this row + if (validSecond.size() != 0) { + + wroteSomething = true; + + out << validSecond[0] << '\t'; + //we are changing the unique name in the fasta file + uniqueMap[firstCol] = validSecond[0]; + + //you know you have at least one valid second since first column is valid + for (int i = 0; i < validSecond.size()-1; i++) { out << validSecond[i] << ','; } + out << validSecond[validSecond.size()-1] << endl; + + if (m->debug) { sanity["name"].insert(validSecond[0]); } + } } } - - gobble(in); + m->gobble(in); } in.close(); out.close(); - if (wroteSomething == false) { - mothurOut("Your file does not contain any sequence from the .accnos file."); mothurOutEndLine(); - remove(outputFileName.c_str()); - } + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["name"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your name file."); m->mothurOutEndLine(); + + return 0; } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "readName"); + m->errorOut(e, "GetSeqsCommand", "readName"); exit(1); } } //********************************************************************************************************************** -void GetSeqsCommand::readGroup(){ +int GetSeqsCommand::readGroup(){ try { - - string outputFileName = getRootName(groupfile) + "pick" + getExtension(groupfile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(groupfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)); + variables["[extension]"] = m->getExtension(groupfile); + string outputFileName = getOutputFileName("group", variables); ofstream out; - openOutputFile(outputFileName, out); + m->openOutputFile(outputFileName, out); + ifstream in; - openInputFile(groupfile, in); + m->openInputFile(groupfile, in); string name, group; bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["group"] = temp; } while(!in.eof()){ + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } + + in >> name; //read from first column in >> group; //read from second column + //if this name is in the accnos file - if (names.count(name) == 1) { + if (names.count(name) != 0) { wroteSomething = true; out << name << '\t' << group << endl; - - names.erase(name); + selectedCount++; + + if (m->debug) { sanity["group"].insert(name); } } - gobble(in); + m->gobble(in); } in.close(); out.close(); - if (wroteSomething == false) { - mothurOut("Your file does not contain any sequence from the .accnos file."); mothurOutEndLine(); - remove(outputFileName.c_str()); - } + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["group"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your group file."); m->mothurOutEndLine(); + + + return 0; } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "readGroup"); + m->errorOut(e, "GetSeqsCommand", "readGroup"); exit(1); } } +//********************************************************************************************************************** +int GetSeqsCommand::readTax(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(taxfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)); + variables["[extension]"] = m->getExtension(taxfile); + string outputFileName = getOutputFileName("taxonomy", variables); + ofstream out; + m->openOutputFile(outputFileName, out); + + ifstream in; + m->openInputFile(taxfile, in); + string name, tax; + + bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["tax"] = temp; } + + while(!in.eof()){ + + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } + in >> name; //read from first column + in >> tax; //read from second column + + if (!dups) {//adjust name if needed + map::iterator it = uniqueMap.find(name); + if (it != uniqueMap.end()) { name = it->second; } + } + + //if this name is in the accnos file + if (names.count(name) != 0) { + wroteSomething = true; + + out << name << '\t' << tax << endl; + selectedCount++; + + if (m->debug) { sanity["tax"].insert(name); } + } + + m->gobble(in); + } + in.close(); + out.close(); + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["taxonomy"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your taxonomy file."); m->mothurOutEndLine(); + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "readTax"); + exit(1); + } +} //********************************************************************************************************************** //alignreport file has a column header line then all other lines contain 16 columns. we just want the first column since that contains the name -void GetSeqsCommand::readAlign(){ +int GetSeqsCommand::readAlign(){ try { - string outputFileName = getRootName(getRootName(alignfile)) + "pick.align.report"; + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(alignfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(alignfile)); + string outputFileName = getOutputFileName("alignreport", variables); ofstream out; - openOutputFile(outputFileName, out); + m->openOutputFile(outputFileName, out); + ifstream in; - openInputFile(alignfile, in); + m->openInputFile(alignfile, in); string name, junk; bool wroteSomething = false; + int selectedCount = 0; //read column headers for (int i = 0; i < 16; i++) { @@ -302,12 +951,21 @@ void GetSeqsCommand::readAlign(){ out << endl; while(!in.eof()){ + + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } + in >> name; //read from first column + + if (!dups) {//adjust name if needed + map::iterator it = uniqueMap.find(name); + if (it != uniqueMap.end()) { name = it->second; } + } //if this name is in the accnos file - if (names.count(name) == 1) { + if (names.count(name) != 0) { wroteSomething = true; + selectedCount++; out << name << '\t'; @@ -318,8 +976,6 @@ void GetSeqsCommand::readAlign(){ } out << endl; - names.erase(name); - }else {//still read just don't do anything with it //read rest for (int i = 0; i < 15; i++) { @@ -328,46 +984,225 @@ void GetSeqsCommand::readAlign(){ } } - gobble(in); + m->gobble(in); } in.close(); out.close(); - if (wroteSomething == false) { - mothurOut("Your file does not contain any sequence from the .accnos file."); mothurOutEndLine(); - remove(outputFileName.c_str()); - } + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["alignreport"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your alignreport file."); m->mothurOutEndLine(); + + return 0; } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "readAlign"); + m->errorOut(e, "GetSeqsCommand", "readAlign"); exit(1); } } //********************************************************************************************************************** +//just looking at common mistakes. +int GetSeqsCommand::runSanityCheck(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); } + string filename = outputDir + "get.seqs.debug.report"; + + ofstream out; + m->openOutputFile(filename, out); + + + //compare fasta, name, qual and taxonomy if given to make sure they contain the same seqs + if (fastafile != "") { + if (namefile != "") { //compare with fasta + if (sanity["fasta"] != sanity["name"]) { //create mismatch file + createMisMatchFile(out, fastafile, namefile, sanity["fasta"], sanity["name"]); + } + } + if (qualfile != "") { + if (sanity["fasta"] != sanity["qual"]) { //create mismatch file + createMisMatchFile(out, fastafile, qualfile, sanity["fasta"], sanity["qual"]); + } + } + if (taxfile != "") { + if (sanity["fasta"] != sanity["tax"]) { //create mismatch file + createMisMatchFile(out, fastafile, taxfile, sanity["fasta"], sanity["tax"]); + } + } + } + + //compare dupnames, groups and list if given to make sure they match + if (namefile != "") { + if (groupfile != "") { + if (sanity["dupname"] != sanity["group"]) { //create mismatch file + createMisMatchFile(out, namefile, groupfile, sanity["dupname"], sanity["group"]); + } + } + if (listfile != "") { + if (sanity["dupname"] != sanity["list"]) { //create mismatch file + createMisMatchFile(out, namefile, listfile, sanity["dupname"], sanity["list"]); + } + } + }else{ -void GetSeqsCommand::readAccnos(){ + if ((groupfile != "") && (fastafile != "")) { + if (sanity["fasta"] != sanity["group"]) { //create mismatch file + createMisMatchFile(out, fastafile, groupfile, sanity["fasta"], sanity["group"]); + } + } + } + + out.close(); + + if (m->isBlank(filename)) { m->mothurRemove(filename); } + else { m->mothurOut("\n[DEBUG]: " + filename + " contains the file mismatches.\n");outputNames.push_back(filename); outputTypes["debug"].push_back(filename); } + + return 0; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "runSanityCheck"); + exit(1); + } +} +//********************************************************************************************************************** +//just looking at common mistakes. +int GetSeqsCommand::createMisMatchFile(ofstream& out, string filename1, string filename2, set set1, set set2){ + try { + out << "****************************************" << endl << endl; + out << "Names unique to " << filename1 << ":\n"; + + //remove names in set1 that are also in set2 + for (set::iterator it = set1.begin(); it != set1.end();) { + string name = *it; + + if (set2.count(name) == 0) { out << name << endl; } //name unique to set1 + else { set2.erase(name); } //you are in both so erase + set1.erase(it++); + } + + out << "\nNames unique to " << filename2 << ":\n"; + //output results + for (set::iterator it = set2.begin(); it != set2.end(); it++) { out << *it << endl; } + + out << "****************************************" << endl << endl; + + return 0; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "runSanityCheck"); + exit(1); + } +} +//********************************************************************************************************************** + +int GetSeqsCommand::compareAccnos(){ try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(accnosfile); } + map variables; + variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(accnosfile)); + string outputFileName = getOutputFileName("accnosreport", variables); + + ofstream out; + m->openOutputFile(outputFileName, out); + ifstream in; - openInputFile(accnosfile, in); + m->openInputFile(accnosfile2, in); string name; + set namesAccnos2; + set namesDups; + set namesAccnos = names; + + map nameCount; + + if (namefile != "") { + ifstream inName; + m->openInputFile(namefile, inName); + + + while(!inName.eof()){ + + if (m->control_pressed) { inName.close(); return 0; } + + string thisname, repnames; + + inName >> thisname; m->gobble(inName); //read from first column + inName >> repnames; //read from second column + + int num = m->getNumNames(repnames); + nameCount[thisname] = num; + + m->gobble(inName); + } + inName.close(); + } + while(!in.eof()){ in >> name; - - names.insert(name); - gobble(in); + if (namesAccnos.count(name) == 0){ //name unique to accnos2 + int pos = name.find_last_of('_'); + string tempName = name; + if (pos != string::npos) { tempName = tempName.substr(pos+1); cout << tempName << endl; } + if (namesAccnos.count(tempName) == 0){ + namesAccnos2.insert(name); + }else { //you are in both so erase + namesAccnos.erase(name); + namesDups.insert(name); + } + }else { //you are in both so erase + namesAccnos.erase(name); + namesDups.insert(name); + } + + m->gobble(in); + } + in.close(); + + out << "Names in both files : " + toString(namesDups.size()) << endl; + m->mothurOut("Names in both files : " + toString(namesDups.size())); m->mothurOutEndLine(); + + for (set::iterator it = namesDups.begin(); it != namesDups.end(); it++) { + out << (*it); + if (namefile != "") { out << '\t' << nameCount[(*it)]; } + out << endl; + } + + out << "Names unique to " + accnosfile + " : " + toString(namesAccnos.size()) << endl; + m->mothurOut("Names unique to " + accnosfile + " : " + toString(namesAccnos.size())); m->mothurOutEndLine(); + + for (set::iterator it = namesAccnos.begin(); it != namesAccnos.end(); it++) { + out << (*it); + if (namefile != "") { out << '\t' << nameCount[(*it)]; } + out << endl; + } + + out << "Names unique to " + accnosfile2 + " : " + toString(namesAccnos2.size()) << endl; + m->mothurOut("Names unique to " + accnosfile2 + " : " + toString(namesAccnos2.size())); m->mothurOutEndLine(); + + for (set::iterator it = namesAccnos2.begin(); it != namesAccnos2.end(); it++) { + out << (*it); + if (namefile != "") { out << '\t' << nameCount[(*it)]; } + out << endl; } - in.close(); + out.close(); + + outputNames.push_back(outputFileName); outputTypes["accnosreport"].push_back(outputFileName); + + return 0; + } catch(exception& e) { - errorOut(e, "GetSeqsCommand", "readAccnos"); + m->errorOut(e, "GetSeqsCommand", "compareAccnos"); exit(1); } } + //**********************************************************************************************************************