X-Git-Url: https://git.donarmstrong.com/?p=mothur.git;a=blobdiff_plain;f=filterseqscommand.cpp;h=8b68988884acb408d0e72c5c3fa407a7b9f241b6;hp=0e33ab2047359b432886384cc41d60a806fbdc7c;hb=050a3ff02473a3d4c0980964e1a9ebe52e55d6b8;hpb=5d176c0f8049654ec3ae5a869c9ee3cecb991dc6 diff --git a/filterseqscommand.cpp b/filterseqscommand.cpp index 0e33ab2..8b68988 100644 --- a/filterseqscommand.cpp +++ b/filterseqscommand.cpp @@ -10,25 +10,99 @@ #include "filterseqscommand.h" #include "sequence.hpp" -/**************************************************************************************/ +//********************************************************************************************************************** +vector FilterSeqsCommand::setParameters(){ + try { + CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","fasta-filter",false,true, true); parameters.push_back(pfasta); + CommandParameter phard("hard", "InputTypes", "", "", "none", "none", "none","",false,false); parameters.push_back(phard); + CommandParameter ptrump("trump", "String", "", "*", "", "", "","",false,false, true); parameters.push_back(ptrump); + CommandParameter psoft("soft", "Number", "", "0", "", "", "","",false,false); parameters.push_back(psoft); + CommandParameter pvertical("vertical", "Boolean", "", "T", "", "", "","",false,false, true); parameters.push_back(pvertical); + CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false, true); parameters.push_back(pprocessors); + CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir); + CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir); + + vector myArray; + for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); } + return myArray; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "setParameters"); + exit(1); + } +} +//********************************************************************************************************************** +string FilterSeqsCommand::getHelpString(){ + try { + string helpString = ""; + helpString += "The filter.seqs command reads a file containing sequences and creates a .filter and .filter.fasta file.\n"; + helpString += "The filter.seqs command parameters are fasta, trump, soft, hard, processors and vertical. \n"; + helpString += "The fasta parameter is required, unless you have a valid current fasta file. You may enter several fasta files to build the filter from and filter, by separating their names with -'s.\n"; + helpString += "For example: fasta=abrecovery.fasta-amazon.fasta \n"; + helpString += "The trump option will remove a column if the trump character is found at that position in any sequence of the alignment. Default=*, meaning no trump. \n"; + helpString += "A soft mask removes any column where the dominant base (i.e. A, T, G, C, or U) does not occur in at least a designated percentage of sequences. Default=0.\n"; + helpString += "The hard parameter allows you to enter a file containing the filter you want to use.\n"; + helpString += "The vertical parameter removes columns where all sequences contain a gap character. The default is T.\n"; + helpString += "The processors parameter allows you to specify the number of processors to use. The default is 1.\n"; + helpString += "The filter.seqs command should be in the following format: \n"; + helpString += "filter.seqs(fasta=yourFastaFile, trump=yourTrump) \n"; + helpString += "Example filter.seqs(fasta=abrecovery.fasta, trump=.).\n"; + helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n"; + return helpString; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "getHelpString"); + exit(1); + } +} +//********************************************************************************************************************** +string FilterSeqsCommand::getOutputPattern(string type) { + try { + string pattern = ""; + + if (type == "fasta") { pattern = "[filename],filter.fasta"; } + else if (type == "filter") { pattern = "[filename],filter"; } + else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; } + + return pattern; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "getOutputPattern"); + exit(1); + } +} +//********************************************************************************************************************** +FilterSeqsCommand::FilterSeqsCommand(){ + try { + abort = true; calledHelp = true; + setParameters(); + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["filter"] = tempOutNames; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "FilterSeqsCommand"); + exit(1); + } +} +/**************************************************************************************/ FilterSeqsCommand::FilterSeqsCommand(string option) { try { - abort = false; + abort = false; calledHelp = false; filterFileName = ""; //allow user to run help - if(option == "help") { help(); abort = true; } + if(option == "help") { help(); abort = true; calledHelp = true; } + else if(option == "citation") { citation(); abort = true; calledHelp = true;} else { - //valid paramters for this command - string Array[] = {"fasta", "trump", "soft", "hard", "vertical", "outputdir","inputdir", "processors"}; - vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + vector myArray = setParameters(); OptionParser parser(option); map parameters = parser.getParameters(); - ValidParameters validParameter; + ValidParameters validParameter("filter.seqs"); map::iterator it; //check to make sure all parameters are valid for command @@ -36,6 +110,11 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; } } + //initialize outputTypes + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["filter"] = tempOutNames; + //if the user changes the input directory command factory will send this info to us in the output parameter string inputDir = validParameter.validFile(parameters, "inputdir", false); if (inputDir == "not found"){ inputDir = ""; } @@ -44,7 +123,7 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { it = parameters.find("fasta"); //user has given a template file if(it != parameters.end()){ - path = hasPath(it->second); + path = m->hasPath(it->second); //if the user has not given a path then, add inputdir. else leave path alone. if (path == "") { parameters["fasta"] = inputDir + it->second; } } @@ -52,7 +131,7 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { it = parameters.find("hard"); //user has given a template file if(it != parameters.end()){ - path = hasPath(it->second); + path = m->hasPath(it->second); //if the user has not given a path then, add inputdir. else leave path alone. if (path == "") { parameters["hard"] = inputDir + it->second; } } @@ -60,31 +139,82 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { //check for required parameters fasta = validParameter.validFile(parameters, "fasta", false); - if (fasta == "not found") { m->mothurOut("fasta is a required parameter for the filter.seqs command."); m->mothurOutEndLine(); abort = true; } + if (fasta == "not found") { + fasta = m->getFastaFile(); + if (fasta != "") { + fastafileNames.push_back(fasta); + m->mothurOut("Using " + fasta + " as input file for the fasta parameter."); m->mothurOutEndLine(); + string simpleName = m->getSimpleName(fasta); + filterFileName += simpleName.substr(0, simpleName.find_first_of('.')); + } + else { m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; } + } else { - splitAtDash(fasta, fastafileNames); + m->splitAtDash(fasta, fastafileNames); //go through files and make sure they are good, if not, then disregard them for (int i = 0; i < fastafileNames.size(); i++) { - if (inputDir != "") { - string path = hasPath(fastafileNames[i]); - //if the user has not given a path then, add inputdir. else leave path alone. - if (path == "") { fastafileNames[i] = inputDir + fastafileNames[i]; } + + bool ignore = false; + if (fastafileNames[i] == "current") { + fastafileNames[i] = m->getFastaFile(); + if (fastafileNames[i] != "") { m->mothurOut("Using " + fastafileNames[i] + " as input file for the fasta parameter where you had given current."); m->mothurOutEndLine(); } + else { + m->mothurOut("You have no current fastafile, ignoring current."); m->mothurOutEndLine(); ignore=true; + //erase from file list + fastafileNames.erase(fastafileNames.begin()+i); + i--; + } } + + if (!ignore) { + if (inputDir != "") { + string path = m->hasPath(fastafileNames[i]); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { fastafileNames[i] = inputDir + fastafileNames[i]; } + } - int ableToOpen; - ifstream in; - ableToOpen = openInputFile(fastafileNames[i], in); - if (ableToOpen == 1) { - m->mothurOut(fastafileNames[i] + " will be disregarded."); m->mothurOutEndLine(); - //erase from file list - fastafileNames.erase(fastafileNames.begin()+i); - i--; - }else{ - string simpleName = getSimpleName(fastafileNames[i]); - filterFileName += simpleName.substr(0, simpleName.find_first_of('.')); + ifstream in; + int ableToOpen = m->openInputFile(fastafileNames[i], in, "noerror"); + + //if you can't open it, try default location + if (ableToOpen == 1) { + if (m->getDefaultPath() != "") { //default path is set + string tryPath = m->getDefaultPath() + m->getSimpleName(fastafileNames[i]); + m->mothurOut("Unable to open " + fastafileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine(); + ifstream in2; + ableToOpen = m->openInputFile(tryPath, in2, "noerror"); + in2.close(); + fastafileNames[i] = tryPath; + } + } + + //if you can't open it, try default location + if (ableToOpen == 1) { + if (m->getOutputDir() != "") { //default path is set + string tryPath = m->getOutputDir() + m->getSimpleName(fastafileNames[i]); + m->mothurOut("Unable to open " + fastafileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine(); + ifstream in2; + ableToOpen = m->openInputFile(tryPath, in2, "noerror"); + in2.close(); + fastafileNames[i] = tryPath; + } + } + + in.close(); + + if (ableToOpen == 1) { + m->mothurOut("Unable to open " + fastafileNames[i] + ". It will be disregarded."); m->mothurOutEndLine(); + //erase from file list + fastafileNames.erase(fastafileNames.begin()+i); + i--; + }else{ + string simpleName = m->getSimpleName(fastafileNames[i]); + filterFileName += simpleName.substr(0, simpleName.find_first_of('.')); + m->setFastaFile(fastafileNames[i]); + } + in.close(); } - in.close(); } //make sure there is at least one valid file left @@ -95,29 +225,33 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { //if the user changes the output directory command factory will send this info to us in the output parameter outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; - outputDir += hasPath(fastafileNames[0]); //if user entered a file with a path then preserve it + outputDir += m->hasPath(fastafileNames[0]); //if user entered a file with a path then preserve it } } //check for optional parameter and set defaults // ...at some point should added some additional type checking... string temp; + hard = validParameter.validFile(parameters, "hard", true); if (hard == "not found") { hard = ""; } + else if (hard == "not open") { hard = ""; abort = true; } + temp = validParameter.validFile(parameters, "trump", false); if (temp == "not found") { temp = "*"; } trump = temp[0]; temp = validParameter.validFile(parameters, "soft", false); if (temp == "not found") { soft = 0; } else { soft = (float)atoi(temp.c_str()) / 100.0; } - temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = "1"; } - convert(temp, processors); - - hard = validParameter.validFile(parameters, "hard", true); if (hard == "not found") { hard = ""; } - else if (hard == "not open") { abort = true; } + temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = m->getProcessors(); } + m->setProcessors(temp); + m->mothurConvert(temp, processors); - vertical = validParameter.validFile(parameters, "vertical", false); if (vertical == "not found") { vertical = "T"; } + vertical = validParameter.validFile(parameters, "vertical", false); + if (vertical == "not found") { + if ((hard == "") && (trump == '*') && (soft == 0)) { vertical = "T"; } //you have not given a hard file or set the trump char. + else { vertical = "F"; } + } numSeqs = 0; - } } @@ -126,75 +260,347 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { exit(1); } } - -//********************************************************************************************************************** - -void FilterSeqsCommand::help(){ - try { - m->mothurOut("The filter.seqs command reads a file containing sequences and creates a .filter and .filter.fasta file.\n"); - m->mothurOut("The filter.seqs command parameters are fasta, trump, soft, hard and vertical. \n"); - m->mothurOut("The fasta parameter is required. You may enter several fasta files to build the filter from and filter, by separating their names with -'s.\n"); - m->mothurOut("For example: fasta=abrecovery.fasta-amazon.fasta \n"); - m->mothurOut("The trump parameter .... The default is ...\n"); - m->mothurOut("The soft parameter .... The default is ....\n"); - m->mothurOut("The hard parameter .... The default is ....\n"); - m->mothurOut("The vertical parameter .... The default is T.\n"); - m->mothurOut("The filter.seqs command should be in the following format: \n"); - m->mothurOut("filter.seqs(fasta=yourFastaFile, trump=yourTrump, soft=yourSoft, hard=yourHard, vertical=yourVertical) \n"); - m->mothurOut("Example filter.seqs(fasta=abrecovery.fasta, trump=..., soft=..., hard=..., vertical=T).\n"); - m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n"); - - } - catch(exception& e) { - m->errorOut(e, "FilterSeqsCommand", "help"); - exit(1); - } -} - /**************************************************************************************/ int FilterSeqsCommand::execute() { try { - if (abort == true) { return 0; } - vector outputNames; + if (abort == true) { if (calledHelp) { return 0; } return 2; } ifstream inFASTA; - openInputFile(fastafileNames[0], inFASTA); + m->openInputFile(fastafileNames[0], inFASTA); Sequence testSeq(inFASTA); alignmentLength = testSeq.getAlignLength(); inFASTA.close(); ////////////create filter///////////////// + m->mothurOut("Creating Filter... "); m->mothurOutEndLine(); filter = createFilter(); + m->mothurOutEndLine(); m->mothurOutEndLine(); + + if (m->control_pressed) { outputTypes.clear(); return 0; } + + #ifdef USE_MPI + int pid; + MPI_Comm_rank(MPI_COMM_WORLD, &pid); + + if (pid == 0) { //only one process should output the filter + #endif + ofstream outFilter; - string filterFile = outputDir + filterFileName + ".filter"; - openOutputFile(filterFile, outFilter); + //prevent giantic file name + map variables; + variables["[filename]"] = outputDir + filterFileName + "."; + if (fastafileNames.size() > 3) { variables["[filename]"] = outputDir + "merge."; } + string filterFile = getOutputFileName("filter", variables); + + m->openOutputFile(filterFile, outFilter); outFilter << filter << endl; outFilter.close(); - outputNames.push_back(filterFile); + outputNames.push_back(filterFile); outputTypes["filter"].push_back(filterFile); + #ifdef USE_MPI + } + #endif ////////////run filter///////////////// + m->mothurOut("Running Filter... "); m->mothurOutEndLine(); + + filterSequences(); + + m->mothurOutEndLine(); m->mothurOutEndLine(); + + int filteredLength = 0; + for(int i=0;icontrol_pressed) { outputTypes.clear(); for(int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; } + + + m->mothurOutEndLine(); + m->mothurOut("Length of filtered alignment: " + toString(filteredLength)); m->mothurOutEndLine(); + m->mothurOut("Number of columns removed: " + toString((alignmentLength-filteredLength))); m->mothurOutEndLine(); + m->mothurOut("Length of the original alignment: " + toString(alignmentLength)); m->mothurOutEndLine(); + m->mothurOut("Number of sequences used to construct filter: " + toString(numSeqs)); m->mothurOutEndLine(); + + //set fasta file as new current fastafile + string current = ""; + itTypes = outputTypes.find("fasta"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); } + } + + m->mothurOutEndLine(); + m->mothurOut("Output File Names: "); m->mothurOutEndLine(); + for(int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); } + m->mothurOutEndLine(); + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "execute"); + exit(1); + } +} +/**************************************************************************************/ +int FilterSeqsCommand::filterSequences() { + try { + numSeqs = 0; - for (int i = 0; i < fastafileNames.size(); i++) { - ifstream in; - openInputFile(fastafileNames[i], in); - string filteredFasta = outputDir + getRootName(getSimpleName(fastafileNames[i])) + "filter.fasta"; - ofstream outFASTA; - openOutputFile(filteredFasta, outFASTA); - outputNames.push_back(filteredFasta); + + for (int s = 0; s < fastafileNames.size(); s++) { + for (int i = 0; i < lines.size(); i++) { delete lines[i]; } lines.clear(); + + map variables; + variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastafileNames[s])); + string filteredFasta = getOutputFileName("fasta", variables); +#ifdef USE_MPI + int pid, numSeqsPerProcessor, num; + int tag = 2001; + vectorMPIPos; + + MPI_Status status; + MPI_Comm_size(MPI_COMM_WORLD, &processors); //set processors to the number of mpi processes running + MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are + + MPI_File outMPI; + MPI_File inMPI; + int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY; + int inMode=MPI_MODE_RDONLY; + + char outFilename[1024]; + strcpy(outFilename, filteredFasta.c_str()); - while(!in.eof()){ - if (m->control_pressed) { in.close(); outFASTA.close(); for(int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } + char inFileName[1024]; + strcpy(inFileName, fastafileNames[s].c_str()); - Sequence seq(in); + MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer + MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI); + + if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; } + + if (pid == 0) { //you are the root process + + MPIPos = m->setFilePosFasta(fastafileNames[s], num); //fills MPIPos, returns numSeqs + numSeqs += num; + + //send file positions to all processes + for(int i = 1; i < processors; i++) { + MPI_Send(&num, 1, MPI_INT, i, tag, MPI_COMM_WORLD); + MPI_Send(&MPIPos[0], (num+1), MPI_LONG, i, tag, MPI_COMM_WORLD); + } + + //figure out how many sequences you have to do + numSeqsPerProcessor = num / processors; + int startIndex = pid * numSeqsPerProcessor; + if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; } + + + //do your part + driverMPIRun(startIndex, numSeqsPerProcessor, inMPI, outMPI, MPIPos); + + if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; } + + //wait on chidren + for(int i = 1; i < processors; i++) { + char buf[5]; + MPI_Recv(buf, 5, MPI_CHAR, i, tag, MPI_COMM_WORLD, &status); + } + + }else { //you are a child process + MPI_Recv(&num, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status); + MPIPos.resize(num+1); + numSeqs += num; + MPI_Recv(&MPIPos[0], (num+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status); + + //figure out how many sequences you have to align + numSeqsPerProcessor = num / processors; + int startIndex = pid * numSeqsPerProcessor; + if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; } + + + //align your part + driverMPIRun(startIndex, numSeqsPerProcessor, inMPI, outMPI, MPIPos); + + if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; } + + char buf[5]; + strcpy(buf, "done"); + + //tell parent you are done. + MPI_Send(buf, 5, MPI_CHAR, 0, tag, MPI_COMM_WORLD); + } + + MPI_File_close(&outMPI); + MPI_File_close(&inMPI); + MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case + +#else + + vector positions; + if (savedPositions.size() != 0) { positions = savedPositions[s]; } + else { +#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) + positions = m->divideFile(fastafileNames[s], processors); +#else + if(processors != 1){ + int numFastaSeqs = 0; + positions = m->setFilePosFasta(fastafileNames[s], numFastaSeqs); + if (positions.size() < processors) { processors = positions.size(); } + } +#endif + } + #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) + //vector positions = m->divideFile(fastafileNames[s], processors); + + for (int i = 0; i < (positions.size()-1); i++) { + lines.push_back(new linePair(positions[i], positions[(i+1)])); + } + + if(processors == 1){ + int numFastaSeqs = driverRunFilter(filter, filteredFasta, fastafileNames[s], lines[0]); + numSeqs += numFastaSeqs; + }else{ + int numFastaSeqs = createProcessesRunFilter(filter, fastafileNames[s], filteredFasta); + numSeqs += numFastaSeqs; + } + + if (m->control_pressed) { return 1; } + #else + if(processors == 1){ + lines.push_back(new linePair(0, 1000)); + int numFastaSeqs = driverRunFilter(filter, filteredFasta, fastafileNames[s], lines[0]); + numSeqs += numFastaSeqs; + }else { + int numFastaSeqs = positions.size()-1; + //positions = m->setFilePosFasta(fastafileNames[s], numFastaSeqs); + + //figure out how many sequences you have to process + int numSeqsPerProcessor = numFastaSeqs / processors; + for (int i = 0; i < processors; i++) { + int startIndex = i * numSeqsPerProcessor; + if(i == (processors - 1)){ numSeqsPerProcessor = numFastaSeqs - i * numSeqsPerProcessor; } + lines.push_back(new linePair(positions[startIndex], numSeqsPerProcessor)); + } + + numFastaSeqs = createProcessesRunFilter(filter, fastafileNames[s], filteredFasta); + numSeqs += numFastaSeqs; + } + + if (m->control_pressed) { return 1; } + #endif +#endif + outputNames.push_back(filteredFasta); outputTypes["fasta"].push_back(filteredFasta); + } + + return 0; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "filterSequences"); + exit(1); + } +} +#ifdef USE_MPI +/**************************************************************************************/ +int FilterSeqsCommand::driverMPIRun(int start, int num, MPI_File& inMPI, MPI_File& outMPI, vector& MPIPos) { + try { + string outputString = ""; + int count = 0; + MPI_Status status; + + for(int i=0;icontrol_pressed) { return 0; } + + //read next sequence + int length = MPIPos[start+i+1] - MPIPos[start+i]; + char* buf4 = new char[length]; + MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status); + + string tempBuf = buf4; + if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); } + istringstream iss (tempBuf,istringstream::in); + delete buf4; + + Sequence seq(iss); m->gobble(iss); + + if (seq.getName() != "") { + string align = seq.getAligned(); + string filterSeq = ""; + + for(int j=0;jerrorOut(e, "FilterSeqsCommand", "driverRunFilter"); + exit(1); + } +} +#endif +/**************************************************************************************/ +int FilterSeqsCommand::driverRunFilter(string F, string outputFilename, string inputFilename, linePair* filePos) { + try { + ofstream out; + m->openOutputFile(outputFilename, out); + + ifstream in; + m->openInputFile(inputFilename, in); + + in.seekg(filePos->start); + + bool done = false; + int count = 0; + + while (!done) { + + if (m->control_pressed) { in.close(); out.close(); return 0; } + + Sequence seq(in); m->gobble(in); if (seq.getName() != "") { string align = seq.getAligned(); string filterSeq = ""; @@ -205,48 +611,149 @@ int FilterSeqsCommand::execute() { } } - outFASTA << '>' << seq.getName() << endl << filterSeq << endl; - numSeqs++; - } - gobble(in); + out << '>' << seq.getName() << endl << filterSeq << endl; + count++; } - outFASTA.close(); - in.close(); + + #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) + unsigned long long pos = in.tellg(); + if ((pos == -1) || (pos >= filePos->end)) { break; } + #else + if (in.eof()) { break; } + #endif + + //report progress + if((count) % 100 == 0){ m->mothurOutJustToScreen(toString(count)+"\n"); } } + //report progress + if((count) % 100 != 0){ m->mothurOutJustToScreen(toString(count)+"\n"); } - int filteredLength = 0; - for(int i=0;icontrol_pressed) { for(int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } + out.close(); + in.close(); + + return count; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "driverRunFilter"); + exit(1); + } +} +/**************************************************************************************************/ +int FilterSeqsCommand::createProcessesRunFilter(string F, string filename, string filteredFastaName) { + try { + + int process = 1; + int num = 0; + processIDS.clear(); + +#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) - m->mothurOutEndLine(); - m->mothurOut("Length of filtered alignment: " + toString(filteredLength)); m->mothurOutEndLine(); - m->mothurOut("Number of columns removed: " + toString((alignmentLength-filteredLength))); m->mothurOutEndLine(); - m->mothurOut("Length of the original alignment: " + toString(alignmentLength)); m->mothurOutEndLine(); - m->mothurOut("Number of sequences used to construct filter: " + toString(numSeqs)); m->mothurOutEndLine(); + //loop through and create all the processes you want + while (process != processors) { + int pid = fork(); + + if (pid > 0) { + processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later + process++; + }else if (pid == 0){ + string filteredFasta = filename + toString(getpid()) + ".temp"; + num = driverRunFilter(F, filteredFasta, filename, lines[process]); + + //pass numSeqs to parent + ofstream out; + string tempFile = filename + toString(getpid()) + ".num.temp"; + m->openOutputFile(tempFile, out); + out << num << endl; + out.close(); + + exit(0); + }else { + m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); + for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); } + exit(0); + } + } - m->mothurOutEndLine(); - m->mothurOut("Output File Names: "); m->mothurOutEndLine(); - for(int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); } - m->mothurOutEndLine(); - - return 0; + num = driverRunFilter(F, filteredFastaName, filename, lines[0]); + + //force parent to wait until all the processes are done + for (int i=0;iopenInputFile(tempFile, in); + if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; } + in.close(); m->mothurRemove(tempFile); + + m->appendFiles((filename + toString(processIDS[i]) + ".temp"), filteredFastaName); + m->mothurRemove((filename + toString(processIDS[i]) + ".temp")); + } + +#else + + ////////////////////////////////////////////////////////////////////////////////////////////////////// + //Windows version shared memory, so be careful when passing variables through the filterData struct. + //Above fork() will clone, so memory is separate, but that's not the case with windows, + //Taking advantage of shared memory to allow both threads to add info to F. + ////////////////////////////////////////////////////////////////////////////////////////////////////// + + vector pDataArray; + DWORD dwThreadIdArray[processors-1]; + HANDLE hThreadArray[processors-1]; + //Create processor worker threads. + for( int i=0; istart, lines[i]->end, alignmentLength, i); + pDataArray.push_back(tempFilter); + processIDS.push_back(i); + + hThreadArray[i] = CreateThread(NULL, 0, MyRunFilterThreadFunction, pDataArray[i], 0, &dwThreadIdArray[i]); + } + + num = driverRunFilter(F, (filteredFastaName + toString(processors-1) + ".temp"), filename, lines[processors-1]); + + //Wait until all threads have terminated. + WaitForMultipleObjects(processors-1, hThreadArray, TRUE, INFINITE); + + //Close all thread handles and free memory allocations. + for(int i=0; i < pDataArray.size(); i++){ + num += pDataArray[i]->count; + if (pDataArray[i]->count != pDataArray[i]->end) { + m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end) + " sequences assigned to it, quitting. \n"); m->control_pressed = true; + } + CloseHandle(hThreadArray[i]); + delete pDataArray[i]; + } + + for (int i = 1; i < processors; i++) { + m->appendFiles((filteredFastaName + toString(i) + ".temp"), filteredFastaName); + m->mothurRemove((filteredFastaName + toString(i) + ".temp")); + } +#endif + + return num; + } catch(exception& e) { - m->errorOut(e, "FilterSeqsCommand", "execute"); + m->errorOut(e, "FilterSeqsCommand", "createProcessesRunFilter"); exit(1); } } /**************************************************************************************/ string FilterSeqsCommand::createFilter() { try { - string filterString = ""; - + string filterString = ""; Filters F; if (soft != 0) { F.setSoft(soft); } @@ -254,7 +761,7 @@ string FilterSeqsCommand::createFilter() { F.setLength(alignmentLength); - if(soft != 0 || isTrue(vertical)){ + if(trump != '*' || m->isTrue(vertical) || soft != 0){ F.initialize(); } @@ -262,79 +769,202 @@ string FilterSeqsCommand::createFilter() { else { F.setFilter(string(alignmentLength, '1')); } numSeqs = 0; - - if(trump != '*' || isTrue(vertical) || soft != 0){ + if(trump != '*' || m->isTrue(vertical) || soft != 0){ for (int s = 0; s < fastafileNames.size(); s++) { for (int i = 0; i < lines.size(); i++) { delete lines[i]; } lines.clear(); + +#ifdef USE_MPI + int pid, numSeqsPerProcessor, num; + int tag = 2001; + vector MPIPos; - #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) - if(processors == 1){ - ifstream inFASTA; - openInputFile(fastafileNames[s], inFASTA); - int numFastaSeqs=count(istreambuf_iterator(inFASTA),istreambuf_iterator(), '>'); - inFASTA.close(); - - numSeqs += numFastaSeqs; - - lines.push_back(new linePair(0, numFastaSeqs)); + MPI_Status status; + MPI_File inMPI; + MPI_Comm_size(MPI_COMM_WORLD, &processors); + MPI_Comm_rank(MPI_COMM_WORLD, &pid); + + //char* tempFileName = new char(fastafileNames[s].length()); + //tempFileName = &(fastafileNames[s][0]); - driverCreateFilter(F, fastafileNames[s], lines[0]); - }else{ - vector positions; + char tempFileName[1024]; + strcpy(tempFileName, fastafileNames[s].c_str()); + + MPI_File_open(MPI_COMM_WORLD, tempFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer - ifstream inFASTA; - openInputFile(fastafileNames[s], inFASTA); + if (m->control_pressed) { MPI_File_close(&inMPI); return 0; } - string input; - while(!inFASTA.eof()){ - input = getline(inFASTA); - if (input.length() != 0) { - if(input[0] == '>'){ long int pos = inFASTA.tellg(); positions.push_back(pos - input.length() - 1); } - } + if (pid == 0) { //you are the root process + MPIPos = m->setFilePosFasta(fastafileNames[s], num); //fills MPIPos, returns numSeqs + numSeqs += num; + + //send file positions to all processes + for(int i = 1; i < processors; i++) { + MPI_Send(&num, 1, MPI_INT, i, tag, MPI_COMM_WORLD); + MPI_Send(&MPIPos[0], (num+1), MPI_LONG, i, tag, MPI_COMM_WORLD); } - inFASTA.close(); - - int numFastaSeqs = positions.size(); + + //figure out how many sequences you have to do + numSeqsPerProcessor = num / processors; + int startIndex = pid * numSeqsPerProcessor; + if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; } - numSeqs += numFastaSeqs; - int numSeqsPerProcessor = numFastaSeqs / processors; + //do your part + MPICreateFilter(startIndex, numSeqsPerProcessor, F, inMPI, MPIPos); + + if (m->control_pressed) { MPI_File_close(&inMPI); return 0; } + + }else { //i am the child process + MPI_Recv(&num, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status); + MPIPos.resize(num+1); + numSeqs += num; + MPI_Recv(&MPIPos[0], (num+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status); + + //figure out how many sequences you have to align + numSeqsPerProcessor = num / processors; + int startIndex = pid * numSeqsPerProcessor; + if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; } + + + //do your part + MPICreateFilter(startIndex, numSeqsPerProcessor, F, inMPI, MPIPos); + + if (m->control_pressed) { MPI_File_close(&inMPI); return 0; } + } - for (int i = 0; i < processors; i++) { - long int startPos = positions[ i * numSeqsPerProcessor ]; - if(i == processors - 1){ - numSeqsPerProcessor = numFastaSeqs - i * numSeqsPerProcessor; - } - lines.push_back(new linePair(startPos, numSeqsPerProcessor)); - } + MPI_File_close(&inMPI); + MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case - createProcessesCreateFilter(F, fastafileNames[s]); - } - #else - ifstream inFASTA; - openInputFile(fastafileNames[s], inFASTA); - int numFastaSeqs=count(istreambuf_iterator(inFASTA),istreambuf_iterator(), '>'); - inFASTA.close(); - - numSeqs += numFastaSeqs; +#else - lines.push_back(new linePair(0, numFastaSeqs)); + vector positions; + #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) + positions = m->divideFile(fastafileNames[s], processors); + for (int i = 0; i < (positions.size()-1); i++) { + lines.push_back(new linePair(positions[i], positions[(i+1)])); + } - driverCreateFilter(F, lines[0], fastafileNames[s]); - #endif + if(processors == 1){ + int numFastaSeqs = driverCreateFilter(F, fastafileNames[s], lines[0]); + numSeqs += numFastaSeqs; + }else{ + int numFastaSeqs = createProcessesCreateFilter(F, fastafileNames[s]); + numSeqs += numFastaSeqs; + } + #else + if(processors == 1){ + lines.push_back(new linePair(0, 1000)); + int numFastaSeqs = driverCreateFilter(F, fastafileNames[s], lines[0]); + numSeqs += numFastaSeqs; + }else { + int numFastaSeqs = 0; + positions = m->setFilePosFasta(fastafileNames[s], numFastaSeqs); + if (positions.size() < processors) { processors = positions.size(); } + + //figure out how many sequences you have to process + int numSeqsPerProcessor = numFastaSeqs / processors; + for (int i = 0; i < processors; i++) { + int startIndex = i * numSeqsPerProcessor; + if(i == (processors - 1)){ numSeqsPerProcessor = numFastaSeqs - i * numSeqsPerProcessor; } + lines.push_back(new linePair(positions[startIndex], numSeqsPerProcessor)); + } + + numFastaSeqs = createProcessesCreateFilter(F, fastafileNames[s]); + numSeqs += numFastaSeqs; + } + #endif + //save the file positions so we can reuse them in the runFilter function + savedPositions[s] = positions; + + if (m->control_pressed) { return filterString; } +#endif + + } + } + + +#ifdef USE_MPI + int pid; + int Atag = 1; int Ttag = 2; int Ctag = 3; int Gtag = 4; int Gaptag = 5; + MPI_Status status; + + MPI_Comm_rank(MPI_COMM_WORLD, &pid); + + if(trump != '*' || m->isTrue(vertical) || soft != 0){ + if (pid == 0) { //only one process should output the filter + vector temp; temp.resize(alignmentLength+1); + + //get the frequencies from the child processes + for(int i = 1; i < processors; i++) { + + for (int j = 0; j < 5; j++) { + + MPI_Recv(&temp[0], (alignmentLength+1), MPI_INT, i, 2001, MPI_COMM_WORLD, &status); + int receiveTag = temp[temp.size()-1]; //child process added a int to the end to indicate what letter count this is for + + if (receiveTag == Atag) { //you are recieveing the A frequencies + for (int k = 0; k < alignmentLength; k++) { F.a[k] += temp[k]; } + }else if (receiveTag == Ttag) { //you are recieveing the T frequencies + for (int k = 0; k < alignmentLength; k++) { F.t[k] += temp[k]; } + }else if (receiveTag == Ctag) { //you are recieveing the C frequencies + for (int k = 0; k < alignmentLength; k++) { F.c[k] += temp[k]; } + }else if (receiveTag == Gtag) { //you are recieveing the G frequencies + for (int k = 0; k < alignmentLength; k++) { F.g[k] += temp[k]; } + }else if (receiveTag == Gaptag) { //you are recieveing the gap frequencies + for (int k = 0; k < alignmentLength; k++) { F.gap[k] += temp[k]; } + } + } + } + }else{ + + //send my fequency counts + F.a.push_back(Atag); + int ierr = MPI_Send(&(F.a[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + F.t.push_back(Ttag); + ierr = MPI_Send (&(F.t[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + F.c.push_back(Ctag); + ierr = MPI_Send(&(F.c[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + F.g.push_back(Gtag); + ierr = MPI_Send(&(F.g[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + F.gap.push_back(Gaptag); + ierr = MPI_Send(&(F.gap[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); } + } + MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case + + if (pid == 0) { //only one process should output the filter +#endif + F.setNumSeqs(numSeqs); - - if(isTrue(vertical) == 1) { F.doVertical(); } + if(m->isTrue(vertical) == 1) { F.doVertical(); } if(soft != 0) { F.doSoft(); } - filterString = F.getFilter(); - + +#ifdef USE_MPI + //send filter string to kids + //for(int i = 1; i < processors; i++) { + // MPI_Send(&filterString[0], alignmentLength, MPI_CHAR, i, 2001, MPI_COMM_WORLD); + //} + MPI_Bcast(&filterString[0], alignmentLength, MPI_CHAR, 0, MPI_COMM_WORLD); + }else{ + //recieve filterString + char* tempBuf = new char[alignmentLength]; + //MPI_Recv(&tempBuf[0], alignmentLength, MPI_CHAR, 0, 2001, MPI_COMM_WORLD, &status); + MPI_Bcast(tempBuf, alignmentLength, MPI_CHAR, 0, MPI_COMM_WORLD); + + filterString = tempBuf; + if (filterString.length() > alignmentLength) { filterString = filterString.substr(0, alignmentLength); } + delete tempBuf; + } + + MPI_Barrier(MPI_COMM_WORLD); +#endif + return filterString; } catch(exception& e) { @@ -343,65 +973,230 @@ string FilterSeqsCommand::createFilter() { } } /**************************************************************************************/ -int FilterSeqsCommand::driverCreateFilter(Filters& F, string filename, linePair* line) { +int FilterSeqsCommand::driverCreateFilter(Filters& F, string filename, linePair* filePos) { try { ifstream in; - openInputFile(filename, in); + m->openInputFile(filename, in); - in.seekg(line->start); - - for(int i=0;inumSeqs;i++){ + in.seekg(filePos->start); + + bool done = false; + int count = 0; + + while (!done) { if (m->control_pressed) { in.close(); return 1; } - Sequence seq(in); + Sequence seq(in); m->gobble(in); if (seq.getName() != "") { - if(trump != '*'){ F.doTrump(seq); } - if(isTrue(vertical) || soft != 0){ F.getFreqs(seq); } + if (seq.getAligned().length() != alignmentLength) { m->mothurOut("Sequences are not all the same length, please correct."); m->mothurOutEndLine(); m->control_pressed = true; } + + if(trump != '*') { F.doTrump(seq); } + if(m->isTrue(vertical) || soft != 0) { F.getFreqs(seq); } cout.flush(); + count++; } + + #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) + unsigned long long pos = in.tellg(); + if ((pos == -1) || (pos >= filePos->end)) { break; } + #else + if (in.eof()) { break; } + #endif + + //report progress + if((count) % 100 == 0){ m->mothurOutJustToScreen(toString(count)+"\n"); } } - + //report progress + if((count) % 100 != 0){ m->mothurOutJustToScreen(toString(count)+"\n"); } in.close(); - return 0; + return count; } catch(exception& e) { m->errorOut(e, "FilterSeqsCommand", "driverCreateFilter"); exit(1); } } +#ifdef USE_MPI +/**************************************************************************************/ +int FilterSeqsCommand::MPICreateFilter(int start, int num, Filters& F, MPI_File& inMPI, vector& MPIPos) { + try { + + MPI_Status status; + int pid; + MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are + + for(int i=0;icontrol_pressed) { return 0; } + + //read next sequence + int length = MPIPos[start+i+1] - MPIPos[start+i]; + + char* buf4 = new char[length]; + MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status); + + string tempBuf = buf4; + if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); } + istringstream iss (tempBuf,istringstream::in); + delete buf4; + + Sequence seq(iss); + + if (seq.getAligned().length() != alignmentLength) { cout << "Alignment length is " << alignmentLength << " and sequence " << seq.getName() << " has length " << seq.getAligned().length() << ", please correct." << endl; exit(1); } + + if(trump != '*'){ F.doTrump(seq); } + if(m->isTrue(vertical) || soft != 0){ F.getFreqs(seq); } + cout.flush(); + + //report progress + if((i+1) % 100 == 0){ cout << (i+1) << endl; } + } + + //report progress + if((num) % 100 != 0){ cout << num << endl; } + + return 0; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "MPICreateFilter"); + exit(1); + } +} +#endif /**************************************************************************************************/ int FilterSeqsCommand::createProcessesCreateFilter(Filters& F, string filename) { try { -#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) - int process = 0; - int exitCommand = 1; - vector processIDS; - + int process = 1; + int num = 0; + processIDS.clear(); + +#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix) + //loop through and create all the processes you want while (process != processors) { - int pid = vfork(); + int pid = fork(); if (pid > 0) { processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later process++; }else if (pid == 0){ - driverCreateFilter(F, filename, lines[process]); + //reset child's filter counts to 0; + F.a.clear(); F.a.resize(alignmentLength, 0); + F.t.clear(); F.t.resize(alignmentLength, 0); + F.g.clear(); F.g.resize(alignmentLength, 0); + F.c.clear(); F.c.resize(alignmentLength, 0); + F.gap.clear(); F.gap.resize(alignmentLength, 0); + + num = driverCreateFilter(F, filename, lines[process]); + + //write out filter counts to file + filename += toString(getpid()) + "filterValues.temp"; + ofstream out; + m->openOutputFile(filename, out); + + out << num << endl; + out << F.getFilter() << endl; + for (int k = 0; k < alignmentLength; k++) { out << F.a[k] << '\t'; } out << endl; + for (int k = 0; k < alignmentLength; k++) { out << F.t[k] << '\t'; } out << endl; + for (int k = 0; k < alignmentLength; k++) { out << F.g[k] << '\t'; } out << endl; + for (int k = 0; k < alignmentLength; k++) { out << F.c[k] << '\t'; } out << endl; + for (int k = 0; k < alignmentLength; k++) { out << F.gap[k] << '\t'; } out << endl; + + //cout << F.getFilter() << endl; + out.close(); + exit(0); - }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); } + }else { + m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); + for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); } + exit(0); + } } + //parent do your part + num = driverCreateFilter(F, filename, lines[0]); + //force parent to wait until all the processes are done - for (int i=0;iopenInputFile(tempFilename, in); + + int temp, tempNum; + string tempFilterString; + + in >> tempNum; m->gobble(in); num += tempNum; + + in >> tempFilterString; + F.mergeFilter(tempFilterString); + + for (int k = 0; k < alignmentLength; k++) { in >> temp; F.a[k] += temp; } m->gobble(in); + for (int k = 0; k < alignmentLength; k++) { in >> temp; F.t[k] += temp; } m->gobble(in); + for (int k = 0; k < alignmentLength; k++) { in >> temp; F.g[k] += temp; } m->gobble(in); + for (int k = 0; k < alignmentLength; k++) { in >> temp; F.c[k] += temp; } m->gobble(in); + for (int k = 0; k < alignmentLength; k++) { in >> temp; F.gap[k] += temp; } m->gobble(in); + + in.close(); + m->mothurRemove(tempFilename); + } + + +#else + + ////////////////////////////////////////////////////////////////////////////////////////////////////// + //Windows version shared memory, so be careful when passing variables through the filterData struct. + //Above fork() will clone, so memory is separate, but that's not the case with windows, + //Taking advantage of shared memory to allow both threads to add info to F. + ////////////////////////////////////////////////////////////////////////////////////////////////////// + + vector pDataArray; + DWORD dwThreadIdArray[processors]; + HANDLE hThreadArray[processors]; + + //Create processor worker threads. + for( int i=0; istart, lines[i]->end, alignmentLength, trump, vertical, soft, hard, i); + pDataArray.push_back(tempFilter); + processIDS.push_back(i); + + hThreadArray[i] = CreateThread(NULL, 0, MyCreateFilterThreadFunction, pDataArray[i], 0, &dwThreadIdArray[i]); + } + + //Wait until all threads have terminated. + WaitForMultipleObjects(processors, hThreadArray, TRUE, INFINITE); + + //Close all thread handles and free memory allocations. + for(int i=0; i < pDataArray.size(); i++){ + num += pDataArray[i]->count; + if (pDataArray[i]->count != pDataArray[i]->end) { + m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end) + " sequences assigned to it, quitting. \n"); m->control_pressed = true; + } + F.mergeFilter(pDataArray[i]->F.getFilter()); + + for (int k = 0; k < alignmentLength; k++) { F.a[k] += pDataArray[i]->F.a[k]; } + for (int k = 0; k < alignmentLength; k++) { F.t[k] += pDataArray[i]->F.t[k]; } + for (int k = 0; k < alignmentLength; k++) { F.g[k] += pDataArray[i]->F.g[k]; } + for (int k = 0; k < alignmentLength; k++) { F.c[k] += pDataArray[i]->F.c[k]; } + for (int k = 0; k < alignmentLength; k++) { F.gap[k] += pDataArray[i]->F.gap[k]; } + + CloseHandle(hThreadArray[i]); + delete pDataArray[i]; + } + +#endif + return num; + } catch(exception& e) { m->errorOut(e, "FilterSeqsCommand", "createProcessesCreateFilter");