# by inspecting the first 100 residues.
def type_guess!
self.type = self.type_guess
+ self
end
# Returns the length of a sequence.
key
end
- # Method to reverse complement sequence.
- def reverse_complement
- self.reverse
- self.complement
- self
+ # Method to reverse the sequence.
+ def reverse
+ Seq.new(self.seq_name, self.seq.reverse, self.type, self.qual ? self.qual.reverse : self.qual)
end
- alias :revcomp :reverse_complement
-
# Method to reverse the sequence.
- def reverse
+ def reverse!
self.seq.reverse!
self.qual.reverse! if self.qual
self
def complement
raise SeqError, "Cannot complement 0 length sequence" if self.length == 0
+ entry = Seq.new
+ entry.seq_name = self.seq_name
+ entry.type = self.type
+ entry.qual = self.qual
+
+ if self.is_dna?
+ entry.seq = self.seq.tr('AGCUTRYWSMKHDVBNagcutrywsmkhdvbn', 'TCGAAYRWSKMDHBVNtcgaayrwskmdhbvn')
+ elsif self.is_rna?
+ entry.seq = self.seq.tr('AGCUTRYWSMKHDVBNagcutrywsmkhdvbn', 'UCGAAYRWSKMDHBVNucgaayrwskmdhbvn')
+ else
+ raise SeqError, "Cannot complement sequence type: #{self.type}"
+ end
+
+ entry
+ end
+
+ # Method that complements sequence including ambiguity codes.
+ def complement!
+ raise SeqError, "Cannot complement 0 length sequence" if self.length == 0
+
if self.is_dna?
self.seq.tr!('AGCUTRYWSMKHDVBNagcutrywsmkhdvbn', 'TCGAAYRWSKMDHBVNtcgaayrwskmdhbvn')
elsif self.is_rna?
else
raise SeqError, "Cannot complement sequence type: #{self.type}"
end
+
+ self
end
# Method to determine the Hamming Distance between
((self.seq.scan(/[a-z]/).size.to_f / (self.len - self.indels).to_f) * 100).round(2)
end
- # Hard masks sequence residues where the corresponding quality score
- # is below a given cutoff.
- def mask_seq_hard_old(cutoff)
- seq = self.seq.upcase
- scores = self.qual
- i = 0
-
- scores.each_char do |score|
- seq[i] = 'N' if score.ord - SCORE_BASE < cutoff
- i += 1
- end
-
- self.seq = seq
- end
-
# Hard masks sequence residues where the corresponding quality score
# is below a given cutoff.
def mask_seq_hard!(cutoff)