#!/usr/bin/env ruby # Copyright (C) 2007-2011 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Write sequence type records to FASTQ files according to a specified key. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/fastq' require 'pp' allowed_enc = 'base_33,base_64' casts = [] casts << {long: 'key', short: 'k', type: 'string', mandatory: true, default: nil, allowed: nil, disallowed: nil} casts << {long: 'dir', short: 'd', type: 'dir!', mandatory: true, default: nil, allowed: nil, disallowed: nil} casts << {long: 'prefix', short: 'p', type: 'string', mandatory: false, default: nil, allowed: nil, disallowed: nil} casts << {long: 'no_stream', short: 'x', type: 'flag', mandatory: false, default: nil, allowed: nil, disallowed: nil} casts << {long: 'compress', short: 'Z', type: 'string', mandatory: false, default: nil, allowed: "gzip,bzip,bzip2", disallowed: nil} casts << {long: 'encoding', short: 'e', type: 'string', mandatory: false, default: 'base_33', allowed: allowed_enc, disallowed: nil} options = Biopieces.options_parse(ARGV, casts) encoding = options[:encoding].to_sym compress = options[:compress] ? options[:compress].to_sym : nil key = options[:key].to_sym fh_hash = {} Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] and record[:SEQ] and record[:SCORES] and record[key] entry = Seq.new_bp(record) entry.qual_convert!(:base_33, encoding) if fh_hash[record[key].to_sym] fastq_io = fh_hash[record[key].to_sym] else if options[:prefix] fastq_file = File.join(options[:dir], [options[:prefix], record[key]].join("_") + ".fastq") else fastq_file = File.join(options[:dir], record[key] + ".fastq") end fastq_file << ".gz" if compress == :gzip fastq_file << ".bz2" if compress == :bzip or compress == :bzip2 fastq_io = Fastq.open(fastq_file, "w", compress: compress) fh_hash[record[key].to_sym] = fastq_io end fastq_io.puts entry.to_fastq end output.puts record unless options[:no_stream] end end fh_hash.each_value { |val| val.close } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__