#!/usr/bin/env ruby # Copyright (C) 2007-2011 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Write sequence type records to FASTQ files according to a specified key. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/fastq' require 'pp' allowed_enc = 'base_33,base_64' casts = [] casts << {:long=>'key', :short=>'k', :type=>'string', :mandatory=>true, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'dir', :short=>'d', :type=>'dir!', :mandatory=>true, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'no_stream', :short=>'x', :type=>'flag', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'encoding', :short=>'e', :type=>'string', :mandatory=>false, :default=>'base_33', :allowed=>allowed_enc, :disallowed=>nil} options = Biopieces.options_parse(ARGV, casts) encoding = options[:encoding].to_sym key = options[:key].to_sym fh_hash = {} Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] and record[:SEQ] and record[:SCORES] and record[key] entry = Seq.new_bp(record) entry.qual_convert!(:base_33, encoding) if fh_hash[record[key].to_sym] fastq_io = fh_hash[record[key].to_sym] else fastq_file = File.join(options[:dir], record[key] + ".fastq") fastq_io = Fastq.open(fastq_file, "w") fh_hash[record[key].to_sym] = fastq_io end fastq_io.puts entry.to_fastq end output.puts record unless options[:no_stream] end end fh_hash.each_value { |val| val.close } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__