#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Write tabular BLAST output from the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Fasta; use Maasha::Biopieces; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, $first, $entry, $data_out ); $options = Maasha::Biopieces::parse_options( [ { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'data_out', short => 'o', type => 'file', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'comment', short => 'c', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'compress', short => 'Z', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); $data_out = Maasha::Biopieces::write_stream( $options->{ "data_out" }, $options->{ "compress" } ) ; $first = 1; while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $entry = biopiece2blast_tab( $record ) ) { if ( $options->{ "comment" } and $first ) { print $data_out "# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score\n"; $first = 0; } print $data_out join( "\t", @{ $entry } ), "\n"; } Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" }; } close $data_out; Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> SUBROUTINES <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< sub biopiece2blast_tab { # Martin A. Hansen, May 2009. # Convert a Biopiece record to a BLAST tabular entry. # Note that we correct positions because BLAST is 1-based. my ( $record, # hashref ) = @_; # Returns a list. my ( @fields, $s_beg, $s_end ); return undef if not defined $record->{ 'REC_TYPE' } or not $record->{ 'REC_TYPE' } eq "BLAST"; if ( $record->{ "STRAND" } eq '+' ) { $s_beg = $record->{ "S_BEG" }; $s_end = $record->{ "S_END" }; } else { $s_beg = $record->{ "S_END" }; $s_end = $record->{ "S_BEG" }; } $fields[ 0 ] = $record->{ "Q_ID" }; $fields[ 1 ] = $record->{ "S_ID" }; $fields[ 2 ] = $record->{ "IDENT" }; $fields[ 3 ] = $record->{ "ALIGN_LEN" }; $fields[ 4 ] = $record->{ "MISMATCHES" }; $fields[ 5 ] = $record->{ "GAPS" }; $fields[ 6 ] = $record->{ "Q_BEG" } + 1; $fields[ 7 ] = $record->{ "Q_END" } + 1; $fields[ 8 ] = $s_beg + 1; $fields[ 9 ] = $s_end + 1; $fields[ 10 ] = $record->{ "E_VAL" }; $fields[ 11 ] = $record->{ "BIT_SCORE" }; return wantarray ? @fields : \@fields; } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__