#!/usr/bin/env perl # Copyright (C) 2007-2010 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Write sequences and scores from stream to FASTA and QUAL files. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Biopieces; use Maasha::Fastq; use Maasha::Fasta; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, $data_out, $qual_out, $entry ); $options = Maasha::Biopieces::parse_options( [ { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'data_out', short => 'o', type => 'file', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'qual_out', short => 'q', type => 'file', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'compress', short => 'Z', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); $data_out = Maasha::Biopieces::write_stream( $options->{ "data_out" }, $options->{ "compress" } ); $qual_out = Maasha::Biopieces::write_stream( $options->{ "qual_out" }, $options->{ "compress" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) ) { Maasha::Fasta::put_entry( $entry, $data_out ); qual_put_entry( $entry->[ 0 ], $record->{ 'SCORES' }, $qual_out ); } Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" }; } close $data_out; close $qual_out; Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< sub qual_put_entry { # Martin A. Hansen, May 2010. # Output a score string in 454 quality format. my ( $name, # entry name $score_str, # scores in Solexa format. $fh, # output filehandle ) = @_; # Returns nothing. $score_str = Maasha::Fastq::solexa_str2dec_str( $score_str ); $score_str =~ tr/;/ /; print $fh ">$name\n"; print $fh "$score_str\n"; } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__