#!/usr/bin/env ruby # Copyright (C) 2007-2011 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Usearch sequences in the stream against a specified database. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/fasta' require 'maasha/usearch' casts = [] casts << {long: 'program', short: 'p', type: 'string', mandatory: false, default: 'global', allowed: "local,global", disallowed: nil} casts << {long: 'database', short: 'd', type: 'file!', mandatory: true, default: nil, allowed: nil, disallowed: nil} casts << {long: 'identity', short: 'i', type: 'float', mandatory: false, default: nil, allowed: nil, disallowed: nil} casts << {long: 'e_val', short: 'e', type: 'float', mandatory: false, default: nil, allowed: nil, disallowed: nil} casts << {long: 'cpus', short: 'c', type: 'uint', mandatory: false, default: 1, allowed: nil, disallowed: "0"} casts << {long: 'maxaccepts', short: 'm', type: 'uint', mandatory: false, default: 0, allowed: nil, disallowed: nil} options = Biopieces.options_parse(ARGV, casts) if options[:program] == 'global' raise ArgumentError, "--identity be specified for global search" unless options[:identity] raise ArgumentError, "--e_val is invalid for global search" if options[:e_val] else raise ArgumentError, "--identity or --e_val must be specified" unless options[:identity] or options[:e_val] end tmpdir = Biopieces.mktmpdir infile = File.join(tmpdir, "in.fna") outfile = File.join(tmpdir, "out.uc") Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| Fasta.open(infile, "w") do |fasta_io| input.each_record do |record| output.puts record if record[:SEQ_NAME] and record[:SEQ] fasta_io.puts Seq.new_bp(record).to_fasta end end end us = Usearch.new(infile, outfile, options) case options[:program] when "local" then us.usearch_local when "global" then us.usearch_global else raise "no such program #{options[:program]}" end us.each_hit do |record| output.puts record end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__