#!/usr/bin/env ruby # Copyright (C) 2007-2013 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Split joined pair-end sequences in the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/seq' require 'pp' options = Biopieces.options_parse(ARGV) Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] and record[:SEQ] and record[:SEQ_LEN_LEFT] and record[:SEQ_LEN_RIGHT] entry = Seq.new_bp(record) len_left = record[:SEQ_LEN_LEFT].to_i len_right = record[:SEQ_LEN_RIGHT].to_i unless len_left + len_right == entry.length raise "SEQ_LEN_LEFT + SEQ_LEN_RIGHT != SEQ_LEN #{len_left} + #{len_right} != #{entry.length}" end entry1 = entry.subseq(0, len_left) entry2 = entry.subseq(len_left) if entry.seq_name =~ /^[^ ]+ \d:/ entry2.seq_name.sub!(/ \d:/, " 2:") elsif entry.seq_name =~ /^.+\/\d$/ entry2.seq_name[-1] = "2" else raise "Could not match sequence name: #{entry.seq_name}" end output.puts entry1.to_bp output.puts entry2.to_bp else output.puts record end end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__