#!/usr/bin/env ruby # Copyright (C) 2007-2011 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Remove columns with indels only from aligned sequences in the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'narray' require 'pp' options = Biopieces.options_parse(ARGV) tmpdir = Biopieces.mktmpdir file_records = File.join(tmpdir, "data.stream") na_mask = false count = 0 Biopieces.open(options[:stream_in], file_records) do |input, output| input.each do |record| if record[:SEQ] na_mask = NArray.int(record[:SEQ].length) unless na_mask na_seq = NArray.to_na(record[:SEQ], "byte") na_mask += na_seq.eq('-'.ord) na_mask += na_seq.eq('.'.ord) na_mask += na_seq.eq('_'.ord) na_mask += na_seq.eq('~'.ord) count += 1 end output.puts record end end na_mask = na_mask.ne count sum = na_mask.sum Biopieces.open(file_records, options[:stream_out]) do |input, output| input.each do |record| if sum > 0 and record[:SEQ] na_seq = NArray.to_na(record[:SEQ], "byte") record[:SEQ] = na_seq[na_mask].to_s record[:SEQ_LEN] = record[:SEQ].length end output.puts record end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__