#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Read SOFT entries from one or more files. # http://www.ncbi.nlm.nih.gov/geo/info/soft2.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Data::Dumper; use Maasha::Biopieces; use Maasha::Filesys; use Maasha::NCBI; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, $data_in, $num, $entry, $records, $soft_index, $fh, @platforms, $plat_table, @samples, $sample, $old_end, $skip, $file ); $options = Maasha::Biopieces::parse_options( [ { long => 'data_in', short => 'i', type => 'files!', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'samples', short => 's', type => 'list', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'num', short => 'n', type => 'uint', mandatory => 'no', default => undef, allowed => undef, disallowed => '0' }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { Maasha::Biopieces::put_record( $record, $out ); } $num = 1; foreach $file ( @{ $options->{ "data_in" } } ) { print STDERR "Creating index for file: $file\n" if $options->{ "verbose" }; $soft_index = Maasha::NCBI::soft_index_file( $file ); # print STDERR Dumper( $soft_index ) if $options->{ "verbose" }; $fh = Maasha::Filesys::file_read_open( $file ); @platforms = grep { $_->{ "SECTION" } =~ /PLATFORM/ } @{ $soft_index }; print STDERR "Getting platform tables for file: $file\n" if $options->{ "verbose" }; $plat_table = Maasha::NCBI::soft_get_platform( $fh, $platforms[ 0 ]->{ "LINE_BEG" }, $platforms[ -1 ]->{ "LINE_END" } ); @samples = grep { $_->{ "SECTION" } =~ /SAMPLE/ } @{ $soft_index }; $old_end = $platforms[ -1 ]->{ "LINE_END" }; foreach $sample ( @samples ) { $skip = 0; $skip = 1 if ( $options->{ "samples" } and grep { $sample->{ "SECTION" } !~ /$_/ } @{ $options->{ "samples" } } ); print STDERR "Getting samples for dataset: $sample->{ 'SECTION' }\n" if $options->{ "verbose" } and not $skip; $records = Maasha::NCBI::soft_get_sample( $fh, $plat_table, $sample->{ "LINE_BEG" } - $old_end - 1, $sample->{ "LINE_END" } - $old_end - 1, $skip ); foreach $record ( @{ $records } ) { Maasha::Biopieces::put_record( $record, $out ); goto NUM if $options->{ "num" } and $num == $options->{ "num" }; $num++; } $old_end = $sample->{ "LINE_END" }; } close $fh; } NUM: close $data_in if $data_in; close $fh if $fh; Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__