#!/usr/bin/env ruby # Copyright (C) 2007-2013 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Read FASTQ entries from one or more files. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/fastq' MAX_TEST = 1_000 allowed_enc = 'auto,base_33,base_64' casts = [] casts << {:long=>'data_in', :short=>'i', :type=>'files!', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'data_in2', :short=>'j', :type=>'files!', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'num', :short=>'n', :type=>'uint', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>'0'} casts << {:long=>'encoding', :short=>'e', :type=>'string', :mandatory=>false, :default=>'auto', :allowed=>allowed_enc, :disallowed=>nil} options = Biopieces.options_parse(ARGV, casts) encoding = options[:encoding].to_sym num = 0 last = false Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| unless options[:data_in] and options[:data_in].first == '-' input.each_record do |record| output.puts record end end if options[:data_in] and options[:data_in2] raise "data_in files differ" if options[:data_in].size != options[:data_in2].size (0 ... options[:data_in].size).each do |i| file1 = options[:data_in][i] file2 = options[:data_in2][i] io1 = Fastq.open(file1, 'r') io2 = Fastq.open(file2, 'r') while entry1 = io1.get_entry and entry2 = io2.get_entry if encoding == :auto if entry1.qual_base33? or entry2.qual_base33? encoding = :base_33 elsif entry1.qual_base64? or entry2.qual_base64? encoding = :base_64 else raise SeqError, "Could not auto-detect quality score encoding" end end entry1.qual_convert!(encoding, :base_33) entry2.qual_convert!(encoding, :base_33) entry1.qual_coerce!(:base_33) entry2.qual_coerce!(:base_33) if num < MAX_TEST raise SeqError, "Quality score outside valid range" unless entry1.qual_valid?(:base_33) raise SeqError, "Quality score outside valid range" unless entry2.qual_valid?(:base_33) end output.puts entry1.to_bp output.puts entry2.to_bp num += 2 if options[:num] and num >= options[:num] last = true break end end io1.close io2.close break if last end elsif options[:data_in] options[:data_in].each do |file| Fastq.open(file, 'r') do |fastq| fastq.each do |entry| if encoding == :auto if entry.qual_base33? encoding = :base_33 elsif entry.qual_base64? encoding = :base_64 else raise SeqError, "Could not auto-detect quality score encoding" end end entry.qual_convert!(encoding, :base_33) entry.qual_coerce!(:base_33) if num < MAX_TEST raise SeqError, "Quality score outside valid range" unless entry.qual_valid?(:base_33) end output.puts entry.to_bp num += 1 if options[:num] == num last = true break end end end break if last end end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__